Alexa Fluor® 647 anti-Glutamine synthetase Antibody, Glutamine synthetase, O91F4

Pricing & Availability

Clone: O91F4 (See other available formats)
Regulatory Status: RUO
Other Names: GLUL, Glutamine Synthetase
Isotype: Mouse IgG2a, κ
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Product Citations: publications

O91F4_A647_Glutamine_IHC-P_072325 — IHC staining with Alexa Fluor® 647 anti-Glutamine synthetase (clone O91F4) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., 1X (Cat. No. 928502), the tissue was incubated without (panel A) or with (panel B) Alexa Fluor® 647 anti-Glutamine synthetase (clone O91F4). Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured on Keyence with a 40X objective and merged. Scale bar: 50 µm

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856203

25 µg

104€

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856204

100 µg

264€

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Description

Glutamine Synthase (GLUL) is primarily expressed in astrocytes in the brain. The main function of GLUL is to catalyze the condensation of glutamate and ammonia to form glutamine. GLUL plays an important role in the metabolic regulation of glutamate, detoxification of brain ammonia, as well as recycling of neurotransmitters. GLUL expression in endothelial cells may be involved in cell migration during pathological angiogenesis. Upregulation of astrocytic GLUL to uptake excess ammonia and glutamate may play a neuroprotective role during neuroinflammation.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human GLUL protein expressed in HEK293T cell
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: IHC-P - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Red Laser (633 nm)
Additional Product Notes: For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704).

Antigen Details

Structure: GLUL is a 373 amino acid protein with an apparent molecular mass of ~ 42 kD.
Distribution: Tissue Distribution: Predominantly expressed in brain, kidney, and liver.
Cellular Distribution: Extracellular, cytosol, nucleus, and mitochondrion.
Interaction: Forms homooctamer and homotetramer.
Cell Type: Astrocytes
Biology Area: Cell Biology, Mitochondrial Function, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
Molecular Family: Postsynaptic proteins, Presynaptic proteins
Antigen References: Eelen G, et al. 2018. Nature. (7721):63-69

Spodenkiewicz M, et al. 2016. Biology (Basel). 5(4)

Fan S, et al. 2018. J Cell Biochem. 119(7):6008
Gene ID: 2752 View all products for this Gene ID 14645 View all products for this Gene ID 24957 View all products for this Gene ID
UniProt: View information about Glutamine synthetase on UniProt.org

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Related Protocols

Immunohistochemistry Protocol for Paraffin-Embedded Sections

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Other Formats

View All Glutamine synthetase Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-Glutamine synthetase	O91F4	WB,IHC-P,ICC
Alexa Fluor® 647 anti-Glutamine synthetase	O91F4	IHC-P

Certificate of Analysis

Go To Top Version: 1 Revision Date: 07.23.2025

For Research Use Only. Not for diagnostic or therapeutic use.

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