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Purified anti-CD73 Antibody

Pricing & Availability
Clone
W23165D (See other available formats)
Regulatory Status
RUO
Other Names
clulster of differentiation 73 (CD73), 5'-Nucleotidase Ecto (NT5E), CALJA, ENT, NT5, EN, E5NT
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A: W23165D_PURE_CD73_ICC_062525
HeLa cells (low-expressing cell line, panel A) and U87-MG cells (positive cell line, panel B) were fixed with 4% PFA Fixation Buffer (Cat. No. 420801) for 10 minutes and permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with Purified anti-CD73 (clone W23165G), followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405405) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 40X objective and merged. Scale bar: 50 µm
  • A: W23165D_PURE_CD73_ICC_062525
    HeLa cells (low-expressing cell line, panel A) and U87-MG cells (positive cell line, panel B) were fixed with 4% PFA Fixation Buffer (Cat. No. 420801) for 10 minutes and permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with Purified anti-CD73 (clone W23165G), followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405405) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 40X objective and merged. Scale bar: 50 µm
  • B: W23165D_PURE_CD73_FC_062525
    Human peripheral blood lymphocytes were stained with PE anti-human CD19 (clone HIB19, Cat. No. 302207) and CD73 Purified (clone W23165D) (left) or Purified rat IgG2a, κ (clone RTK2758, Cat. No. 400501) Isotype Control (right), followed by Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416).
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640251 25 µg 104€
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640252 100 µg 264€
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Description

CD73 (cluster of differentiation 73) is a membrane-bound glycoprotein and ectoenzyme. To activate adenosine signaling CD73 catalyzes the hydrolysis of AMP generated by CD39 to adenosine. This role makes CD73 crucial to the regulation of purinergic signaling and immune homeostasis and response. CD73 also serves a variety of physiological functions including lymphocyte binding, epithelial ion transport, ischemic preconditioning, tissue-barrier function, T cell activation, and tissue homeostasis. Overexpressed in several cancers and implicated in tumor progression, metastasis, and the impairment of anti-tumor immune response via adenosine signaling, CD73 is a target of interest in cancer therapy. It is also a potential immunotherapy target for the treatment of pain, inflammation, and hypoxia.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant protein corresponding to the nucleotidase domain of human CD73
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.03 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with the following:

-Fixation buffer (Cat. No. 420801) with 0.5 % Triton X-100
-Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol
-100% ice-cold methanol only

This product is not recommended for use in immunohistochemistry in formalin fixed, paraffin-embedded tissues (IHC-P) or Western blot (WB). 

Antigen Details

Structure
CD73 is a 574 amino acid protein with a predicted molecular weight of 63 kD.
Distribution

Cell membrane

Function
Hydrolase, metabolic protein, CD marker, enzyme
Interaction
AP-2, SMAD, SP-1, CD39, GSDMD
Ligand/Receptor
Metal-binding, nucleotide-binding, zinc
Cell Type
Lymphocytes, T cells
Biology Area
Cell Biology
Molecular Family
CD Molecules, Enzymes and Regulators, Innate Immune Signaling
Antigen References
  1. Knapp K, et al. 2012. Structure. 20:2161.
  2. Minor M, et al. 2019. Am. J. Physiol. Cell Physiol. 317:C1079.
  3. Yu M, et al. 2020. Nat. Commun. 11:515.
Gene ID
4907 View all products for this Gene ID
UniProt
View information about CD73 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06.25.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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