Alexa Fluor® 488 anti-JunB Antibody, W20103F

Pricing & Availability

Clone: W20103F (See other available formats)
Regulatory Status: RUO
Other Names: Activator Protein 1, AP-1
Isotype: Rat IgG2a, κ
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Product Citations: publications

W20103F_A488_JunB_FC_090825 — HDLM-2 cells (positive cell line, filled histogram) and NTERA-2 cells (low-expressing cell line, open histogram) were fixed and permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401) and intracellularly stained with Alexa Fluor® 488 anti-JunB (clone W20103F) or Alexa Fluor® 488 Mouse IgG2a, κ Isotype Control (dashed histogram, representative of both the positive and negative cell lines).

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600453

25 tests

112€

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600454

100 tests

280€

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Description

JunB is a member of the AP-1 family of transcription factors, which also includes c-Jun, c-Fos, Fra1, and JunD. JunB can form dimers with other Jun and Fos proteins, as well as members of the ATF transcription factor family, to form a functional AP-1 transcription factor complex. AP-1 complexes containing JunB can exert both activator and repressor function depending on the interacting partner and promoter sequence context. JunB displays both tumor suppressor and oncogenic activity, inducing genes involved in cell senescence and repressing genes involved in proliferation. JunB is required for T helper type 2 (Th2), T helper 17 (Th17) cell and regulatory T cell (Treg) differentiation. Multiple cancers display aberrant JunB expression and function. In some lymphomas, notably Hodgkin’s lymphoma, JunB expression is elevated and the protein functions as an oncogenic transcription factor. Conversely, loss of JunB is observed in chronic myeloid leukemia.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human JunB protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool).
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Blue Laser (488 nm)
Additional Product Notes: For use in intracellular flow cytometry (ICFC), it is recommended to fix/permeabilize with True-Phos™ Perm Buffer (Cat. No. 425401).

Antigen Details

Structure: JunB is a 347 amino acid protein with a predicted molecular weight of 36 kD.
Distribution: Nucleus
Function: Transcription Factor
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Cell Proliferation and Viability, Transcription Factors
Antigen References: Shaulian E and Karin M. 2002. Nat Cell Biol. 4:E131-6.

Yang MY, et al. 2003. Blood. 101:3205-11.

Carr T, et al. 2017. Nat Commun. 8:301.

Katagiri T, et al. 2019. Mucosal Immunol. 12:1104-1117.
Gene ID: 3726 View all products for this Gene ID
UniProt: View information about JunB on UniProt.org

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Other Formats

View All JunB Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-JunB	W20103F	ICC,ICFC
Alexa Fluor® 488 anti-JunB Antibody	W20103F	ICFC
Alexa Fluor® 647 anti-JunB	W20103F	ICC,ICFC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 08.06.2025

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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