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Cell-Vive™ GMP Recombinant Human IL-22 (carrier-free)

Pricing & Availability
Other Names
IL22, IL-D110, IL-TIF, ILTIF, MGC79382, MGC79384, TIFIL-23, TIFa, zcyto18
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Product Citations
publications
CV_GMP_RECOM_Human_IL-22_CF_101325
Cell-Vive™ GMP Recombinant Human IL-22 induces IL-10 production in human Colo205 cells in a dose-dependent manner
  • CV_GMP_RECOM_Human_IL-22_CF_101325
    Cell-Vive™ GMP Recombinant Human IL-22 induces IL-10 production in human Colo205 cells in a dose-dependent manner
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571314 25 µg 760€
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571316 100 µg 1816€
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Description

IL-22 is a cytokine structurally related to IL-10. It was originally identified in mouse as a gene induced by IL-9 in T cells and mast cells. IL-22 was initially designated as ILTIF (IL-10-related T cell-derived inducible factor). IL-22 belongs to a family of cytokines with limited homology to IL-10, namely IL-10, IL-19, IL-20, IL-24, IL-26, IL-28A, IL-28B and IL-29 (the later three also known as IFN-λ). Human IL-22 shares 79% amino acid identity with mouse IL-22 and 25% identity with human IL-10. IL-22 biological activity is initiated by the binding to a cell surface complex composed of IL-22R1 and IL-10R2 receptor chains and further regulated by interactions with a soluble binding protein, IL-22BP, which share sequence similarity with an extracellular region of IL-22R1 (sIL-22R1). Both chains of the IL-22R complex belong to the class II CRF. Two types of IL-22 binding receptors have been discovered, a membrane-bound receptor and a soluble receptor, both encoded by different genes. IL-22 is produced by immune cells and acts on nonimmune cells to regulate local tissue inflammation. As a product of T helper 17 lineage of CD4 (+) effector lymphocytes, IL-22 plays a critical role in mucosal immunity as well as in deregulated inflammation observed in autoimmune diseases.

Product Details
Quality Statement

BioLegend Cell-Vive™ GMP Recombinant proteins are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12 in a dedicated GMP facility compliant with ISO 13485:2016. Specifications and processes include:

  • Low endotoxin level (≤ 0.1 EU/µg)
  • Purity (≥ 95% or higher)
  • Bioburden testing
  • Mycoplasma testing
  • Batch-to-batch consistency
  • Vendor qualification
  • Raw material traceability and documentation
  • Documented procedures and employee training
  • Equipment maintenance and monitoring records
  • Lot-specific certificates of analysis
  • Quality audits per ISO 13485:2016
  • QA review of released products
Technical data sheet

Product Details

Source
Human IL-22, amino acids Ala34-Ile179 (Accession # NM_020525) was expressed in E. coli.
Molecular Mass
The 147 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 16 kDa. The DTT-reduced protein migrates at approximately 15 kDa and the non-reduced protein migrates at approximately 14 kDa by SDS-PAGE.
N-terminal
Sequence Analysis
Met-Ala-Pro-Ile-Ser-Ser-His-(Cys)-Arg-Leu
Purity
≥ 95%, as determined by Coomassie stained SDS-PAGE
Formulation
0.1 μm filtered protein solution is in 10 mM Sodium Phosphate buffer, 150 mM NaCl, pH 7.2.
Endotoxin Level
Less than or equal to 0.1 EU per μg of protein as determined by LAL method.
Residual Host Cell Protein Content
≤ 1 ng/μg by ELISA
Concentration
25 μg & 100 μg sizes are bottled at 0.2 mg/mL
Storage & Handling
Unopened vials can be stored at ≤ -70°C for 4 years, ≤ -20°C for 6 months, and between 2°C and 8°C for 2 weeks. Aliquots can be stored between -20°C to -70°C for 3 months and between 2°C and 8°C for 1 week. For maximum results, quick spin vial prior to opening. Stock solutions can also be prepared at 50 - 100 μg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Avoid repeated freeze/thaw cycles.
Activity
Cell-Vive™ GMP Recombinant Human IL-22 induces IL-10 production in human Colo205 cells in a dose-dependent manner. The ED50 for this effect is 0.06 – 0.42 ng/mL.
Application

Bioassay
Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech-BioLegend@revvity.com.

Application References

(PubMed link indicates BioLegend citation)
  1. Dhiman R, et al. 2009. J. Immunol. 183:6639. Pubmed
Disclaimer

BioLegend Cell-Vive™ GMP Recombinant proteins are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

Antigen Details

Structure
Cytokine
Distribution

Thymic lymphomas, T cells, activated T cells, macrophages, and mast cells

Function
Enhances the production of acute phase proteins in a hepatocyte cell line, HepG2; Promotes Th17 cells to transmigrate through the blood-brain barrier in multiple sclerosis
Interaction
Interacts with a cell surface complex composed of IL-22R1 and IL-10R2 receptor chains which are expressed in epithelial cells of inflammatory diseases such as psoriasis, Crohn's disease, rheumatoid arthritis, or allergic asthma
Ligand/Receptor
IL-22R1 and IL-10R2 receptor chains
Bioactivity
IL-22 bioactivity was measured by its ability to induce IL-10 in human Colo205 cells
Antigen References
  1. Nagalakshmi ML, et al. 2004. Int Immunopharmacol. 4:679-691.
  2. Kebir H, et al. 2007. Nat. Med. 13:1173-1175.
  3. Gu Y, et al. 2008. Eur. J. Immunol. 38:1807-1813.
  4. Pene J, et al. 2008. J. Immunol. 180:7423-7430.
  5. Dumotier L, et al. 2000. J immune. 164:1814-1819.
  6. Xie MH, et al. 2000. J. Biol. Chem. 765:31335-31339.
  7. Jones BC, et al. 2008. Structure. 16:1333-44.
  8. Kotenko SV, et al. 2001. J. Immunol. 166:7096-71033.
  9. Chang C, et al. 2006. Cell Res. 16:902-907.
Gene ID
50616 View all products for this Gene ID
UniProt
View information about IL-22 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11.04.2025

For Research Use Only. Suitable for ex vivo cell processing use. Not for injection or diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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