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Intracellular Staining Permeabilization Wash Buffer (10X)

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RUO
Other Names
Perm/Wash, Permeabilization Buffer
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421002 100 mL 76€
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Description

Intracellular Staining Permeabilization Wash Buffer is useful for intracellular staining procedures, e.g., in preparation of cells for staining intracellular cytokines or other proteins. Intracellular Staining Permeabilization Wash Buffer is used to permeabilize cells following fixation with Intracellular Staining Fixation Buffer (Cat. No. 420801). It is supplied as a 10X solution and should be diluted in deionized water prior to use. Intracellular Staining Permeabilization Wash Buffer has been formulated to have minimal effects on cells, reduce non-specific staining and enhance the signal to noise ratio. It can be used for antibody dilutions and cell washing during intracellular staining.

Product Details
Technical data sheet

Product Details

Storage & Handling
The Intracellular Staining Perm wash buffer solution should be stored between 2°C and 8°C. Do not freeze.
Application

ICC, ICFC - Quality tested
IHC - Reported in the literature, not verified in house
Recommended Usage

For use in permeabilization, dilute Intracellular Staining Permeabilization Wash Buffer (10X) to 1X in DI water. Resuspend fixed cells in diluted Intracellular Staining Permeabilization Wash Buffer and centrifuge at 350 xg for 5-10 minutes, and repeat the process twice. It is recommended that the reagent be titrated for optimal performance for each application. Please see "Intracellular Cytokine Staining Protocol" on BioLegend's website for more details.

Application Notes

The Intracellular Staining Permeabilization Wash Buffer (10X) may have crystallization or precipitation observed when it is stored at 2-8°C; however, this is normal and does not affect the buffer's performance. If there is heavy precipitation observed after dilution to 1X working solution, the buffer can be filtered to clarify the solution.

Application References

(PubMed link indicates BioLegend citation)
  1. Smeltz RB. 2007. J. Immunol. 178:4786.
  2. Kang YJ, et al. 2007. Nature Immunol. 8:601.
  3. del Rio ML, et al. 2011. Transplantation. 92:1085. PubMed
  4. del Rio ML, et al. 2012. J. Immunol. 188:4885. PubMed
  5. Marongiu L, et al. 2013. PLoS One. 8:75684. PubMed
  6. Xiao Z, et al. 2013. Mol Immunol. 56:423. PubMed
  7. Kusner LL, et al. 2014. PLoS One. 9:102231. PubMed
  8. Ni PP, et al. 2014. J Immunol. 193:1778. PubMed
Product Citations
  1. Darzianiazizi M, et al. 2020. Int J Mol Sci. 21:00. PubMed
  2. Kim S, et al. 2020. Development. 147:00:00. PubMed
  3. Dumauthioz N, et al. 2020. Cell Mol Immunol. . PubMed
  4. Rappazzo CG, et al. 2020. bioRxiv. . PubMed
  5. Li B, Schmidt N 2016. PLoS One. 11: 0162427. PubMed
  6. Arbues A, et al. 2020. PLoS Pathog. 16:e1008312. PubMed
  7. Kim J, et al. 2021. Cytometry A. 99:807. PubMed
  8. Sulaj A, et al. 2022. J Clin Endocrinol Metab. 107:2167. PubMed
  9. Brog RA, et al. 2022. Cancer Immunol Res. 10:962. PubMed
  10. Nozaki K, et al. 2022. Nature. 606:960. PubMed
  11. Shemesh A, et al. 2022. J Exp Med. 219: . PubMed
  12. Yin XY, et al. 2023. Neural Regen Res. 18:213. PubMed
  13. Blanas A, et al. 2022. iScience. 25:104719. PubMed
  14. Bates TA, et al. 2022. Cell Rep. 41:111544. PubMed
  15. Gong YY, et al. 2022. Cancer Res Commun. 2:842. PubMed
  16. Liang Y, et al. 2022. Theranostics. 12:7729. PubMed
  17. Wang Y, et al. 2022. Front Endocrinol (Lausanne). 13:1036517. PubMed
  18. Koutník J, et al. 2022. Front Immunol. 13:1049033. PubMed
  19. Minott JA, et al. 2022. Front Immunol. 13:1038340. PubMed
  20. Tan TCJ, et al. 2022. Nat Commun. 13:7796. PubMed
  21. Phares TW, et al. 2022. Front Pharmacol. 13:1029636. PubMed
  22. Zhao X, et al. 2022. STAR Protoc. 3:101859. PubMed
  23. Chan L, et al. 2022. Cells. 12: . PubMed
  24. Ding D, et al. 2023. J Med Chem. 66:1467. PubMed
  25. Qin S, et al. 2023. Acta Pharm Sin B. :. PubMed
  26. Sun L, et al. 2023. Mol Med Rep. 27: . PubMed
  27. Di Benedetto C, et al. 2023. Cancers (Basel). 15: . PubMed
  28. Chan L, et al. 2023. Int J Mol Sci. 24: . PubMed
  29. Xiao JF, et al. 2022. Mol Cancer Res. 20:637. PubMed
  30. Shen JZ, et al. 2022. Mol Cell. 82:1123. PubMed
  31. Guo H, et al. 2022. Biomaterials. 283:121441. PubMed
  32. Zhang H, et al. 2022. J Exp Med. 219:. PubMed
  33. Bronge M, et al. 2022. Sci Adv. 8:eabn1823. PubMed
  34. Zhuo Y, et al. 2022. J Immunother Cancer. 10:. PubMed
  35. Chen R, et al. 2022. J Mol Cell Biol. 14:. PubMed
  36. Tang J, et al. 2022. Sci Immunol. 7:eadd4853. PubMed
  37. Shen M, et al. 2022. Adv Sci (Weinh). 9:e2203523. PubMed
  38. Hajnik RL, et al. 2022. Sci Transl Med. 14:eabq1945. PubMed
  39. Frost JN, et al. 2022. Sci Adv. 8:eabq5384. PubMed
  40. Li L, et al. 2022. Cancer Immunol Res. 10:1475. PubMed
  41. Deák P, et al. 2022. Cell Rep. 41:111563. PubMed
  42. Stokes JV, et al. 2022. Curr Protoc. 2:e584. PubMed
  43. Jia DJ, et al. 2022. Gut Microbes. 14:2145843. PubMed
  44. Gao L, et al. 2023. J Nanobiotechnology. 21:56. PubMed
  45. Kodama N, et al. 2023. Front Nutr. 10:1081263. PubMed
  46. Mohamed GA, et al. 2023. Breast Cancer Res. 25:23. PubMed
  47. Varun K, et al. 2023. EBioMedicine. 90:104516. PubMed
  48. Wang YH, et al. 2023. Int J Mol Sci. 24:. PubMed
  49. Deng G, et al. 2023. Cancers (Basel). 15:. PubMed
  50. Wang Y, et al. 2023. iScience. 26:106630. PubMed
  51. Changsheng H, et al. 2023. Sci Adv. 9:eade4186. PubMed
  52. Ruan H, et al. 2023. Acta Pharm Sin B. 13:2202. PubMed
  53. Zhang TR, et al. 2023. Pharmaceuticals (Basel). 16:. PubMed
  54. Komoto S, et al. 2023. Cancers (Basel). 15:. PubMed
  55. Hanga MP, et al. 2021. Biotechnol Bioeng. 118:3175. PubMed
  56. Lentucci C, et al. 2017. J Biol Chem. 292:2754-2772. PubMed
  57. Glaser K, et al. 2015. Immunol Lett. 166: 19-27. PubMed
  58. Kusner L, et al. 2014. PLoS One. 9:102231. PubMed
  59. Khayrullina G, et al. 2020. Skelet Muscle. 10:16. PubMed
  60. Spitzer SO, et al. 2019. Neuron. 101:459. PubMed
  61. Li Q, et al. 2022. Cell Rep. 40:111308. PubMed
  62. van Vloten JP, et al. 2022. J Immunother Cancer. 10:. PubMed
  63. Lebedeva IV, et al. 2021. Cell Death Dis. 12:770. PubMed
  64. Zhang L, et al. 2021. J Immunol. 207:590. PubMed
  65. Li X, et al. 2022. Nat Commun. 13:2794. PubMed
  66. Li L,et al. 2017. Sci Rep.. 10.1038/s41598-017-14000-z. PubMed
  67. Zhang Y, et al. 2020. Oncol Lett. 1.053472222. PubMed
  68. Stewart CA, et al. 2020. bioRxiv. . PubMed
  69. Stewart CA, et al. 2021. J Thorac Oncol. Online ahead of prin. PubMed
  70. Zhang S, et al. 2022. Cell Death Dis. 13:844. PubMed
  71. Huang X, et al. 2019. Cancer Immunol Res. 1.388888889. PubMed
  72. Angiari S, et al. 2020. Cell Metab. 31:391. PubMed
  73. Jacob A, et al. 2020. Nature Protocols. 14(12):3303-3332.. PubMed
  74. Kiritsy MC, et al. 2021. Elife. 10:. PubMed
  75. Poli V, et al. 2021. iScience. 24:103256. PubMed
  76. Duan Q, et al. 2021. Front Cell Dev Biol. 9:761193. PubMed
  77. Stegelmeier AA, et al. 2022. Biomedicines. 10:. PubMed
  78. Gao F, et al. 2021. Front Cell Infect Microbiol. 10:593805. PubMed
  79. Haque M, et al. 2021. STAR Protoc. 2:100264. PubMed
  80. Martinez-Usatorre A, et al. 2020. Front Immunol. 11:340. PubMed
  81. Martinez–Usatorre A, et al. 2019. Cancer Immunol Res. 0.995138889. PubMed
  82. Tsai CY, et al. 2022. J Biomed Sci. 29:47. PubMed
  83. Gärtner F, et al. 2021. Front Immunol. 12:745132. PubMed
  84. Goyal S, et al. 2017. Sci Rep. . 10.1038/s41598-017-18046-x. PubMed
  85. Tang B, et al. 2020. Clin Cancer Res. 26:2216. PubMed
  86. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  87. Herda S, et al. 2021. Int J Cancer. 148:3097. PubMed
  88. Haryadi L, et al. 2019. Vet World. 12:1529. PubMed
  89. Ghose J, et al. 2018. Oncoimmunology. 7:e1486948. PubMed
  90. Lau H, et al. 2021. Int J Mol Sci. 22:. PubMed
  91. Zhou H, et al. 2022. J Clin Invest. 132:. PubMed
  92. Eslani M,et al. 2017. Invest Ophthalmol Vis Sci.. 58(12):5507-5517. PubMed
  93. Shinde MY, et al. 2017. J Biol Chem. 292:18240. PubMed
  94. Jiang P, et al. 2021. Nat Methods. 18:1181. PubMed
  95. Zhan Y, et al. 2021. JCI Insight. 6:. PubMed
  96. Hendricks A, et al. 2021. Front Oncol. 11:646885. PubMed
  97. Shamsi F, et al. 2021. Nat Metab. 3:485. PubMed
  98. Zhang X, et al. 2020. J Immunol. 205:1743. PubMed
  99. Ni P, et al. 2014. J Immunol . 193:1778. PubMed
  100. Subham S, et al. 2022. Breast Cancer Res Treat. Online ahead of print. PubMed
  101. Sohrabi Y, et al. 2020. Front Immunol. 11:353. PubMed
  102. Lau H, et al. 2021. Xenotransplantation. 28:e12667. PubMed
  103. Su W, et al. 2022. Front Immunol. 13:952338. PubMed
  104. Ma Q, et al. 2022. Nat Commun. 13:4142. PubMed
  105. Datta A, et al. 2022. J Fungi (Basel). 8:. PubMed
  106. Martinez–Usatorre A, et al. 2018. J Immunol. 201:792. PubMed
  107. Cheng K, et al. 2021. Nat Commun. 12:2041. PubMed
  108. Xiao Z, et al. 2013. Mol Immunol. 56:423. PubMed
  109. Hilpert C, et al. 2016. J Immunol. 197: 2780 - 2786. PubMed
  110. Zhang X, et al. 2022. Theranostics. 12:1621. PubMed
  111. Berical A, et al. 2022. Nat Commun. 13:4270. PubMed
  112. Clancy–Thompson E, et al. 2018. Cancer Immunol Res. 1.308333333. PubMed
  113. Chen L, et al. 2021. Cell Death Differ. 28:1880. PubMed
  114. Pearce V, et al. 2015. J Immunol. 195: 3206 - 3217. PubMed
  115. Zhang L, et al. 2021. Methods Mol Biol. 2388:175. PubMed
  116. Rodriguez S, et al. 2020. Xenotransplantation. 27:e12554. PubMed
  117. Chakraborty S, et al. 2022. Sci Transl Med. . PubMed
  118. Leonard NA, et al. 2021. Cancers (Basel). 13:. PubMed
  119. Baek J, et al. 2017. Am J Pathol. 10.1016/j.ajpath.2017.02.011. PubMed
  120. Mkhikian H, et al. 2022. Nat Aging. 2:231. PubMed
  121. Li J, et al. 2022. J Tissue Eng. 13:20417314221127908. PubMed
  122. Lau H, et al. 2020. Xenotransplantation. 27:e12555. PubMed
  123. Lownik JC, et al. 2019. J Immunol. 202:664. PubMed
  124. van Duijn A, et al. 2021. Cancer Immunol Immunother. Online ahead of print. PubMed
  125. Jakic B, et al. 2021. Cell Death Dis. 12:187. PubMed
  126. Ouyang W, et al. 2021. Invest Ophthalmol Vis Sci. 62:25:00. PubMed
  127. Nausch N, et al. 2017. PLoS Negl Trop Dis. 11(2):e0005415. PubMed
  128. Xu Z, et al. 2016. Nat Commun. 7:10728. PubMed
  129. Ren Y, et al. 2022. J Immunother Cancer. 10:. PubMed
  130. Wei W, et al. 2022. mSystems. 7:e0046922. PubMed
  131. Zhao X, et al. 2022. Nat Protoc. 17:2240. PubMed
  132. Yang J, et al. 2022. Microbiome. 10:149. PubMed
  133. Alvandi Z, et al. 2022. J Am Heart Assoc. 11:e023695. PubMed
  134. Breznik JA, et al. 2018. Physiol Rep. 6:e13937. PubMed
  135. Page N, et al. 2021. Nat Commun. 12:1009. PubMed
  136. Gunasena M, et al. 2022. Sci Rep. 12:12377. PubMed
  137. Li Q, et al. 2017. Front Immunol. 0.797916667. PubMed
  138. Henrich IC, et al. 2021. Cancer Res. 81:2171. PubMed
  139. Liu X, et al. 2016. Sci Rep. 6:36722. PubMed
  140. Hayes D, et al. 2022. JCI Insight. 7:. PubMed
  141. Rio M, et al. 2012. J Immunol. 188:4885. PubMed
  142. Kundu K, et al. 2019. Cancer Immunol Res. 1.069444444. PubMed
  143. Gong C, et al. 2021. Mol Cell. 81:4059. PubMed
  144. Chen DL, et al. 2021. Mol Cancer. 20:166. PubMed
  145. Kim S, et al. 2020. Development. . PubMed
  146. Bajpai G, et al. 2018. Nat Med. 24:1234. PubMed
  147. Janela B, et al. 2019. Immunity. 50:1069. PubMed
  148. Wilmes A, et al. 2017. Toxicol In Vitro. 10.1016/j.tiv.2017.07.023. PubMed
  149. Hiza H, et al. 2021. Sci Rep. 11:13190. PubMed
  150. Chen YF, et al. 2019. J Biomed Sci. 26:85. PubMed
  151. Rio M, et al. 2011. Transplantation. 92:1085. PubMed
  152. Wen T, et al. 2022. Cancer Immunol Res. 10:162. PubMed
  153. Ma K, et al. 2022. iScience. 25:104347. PubMed
  154. Wang Y, et al. 2020. Virol Sin. 36:122. PubMed
  155. Lau H, et al. 2020. Islets. 41:12. PubMed
  156. Lau H, et al. 2020. PLoS One. 15:e0243506. PubMed
  157. Dong M, et al. 2022. J Immunother Cancer. 10:. PubMed
  158. Wang G, et al. 2020. Nat Commun. 0.611111111. PubMed
  159. Shemesh A, et al. 2022. J Exp Med. 219:. PubMed
  160. Teh MR, et al. 2021. Front Immunol. 12:714613. PubMed
  161. Haswell JR, et al. 2021. PLoS One. 16:e0252848. PubMed
  162. Park SR, et al. 2020. Cell Rep. 32:108077. PubMed
  163. Chen RH, et al. 2021. Front Immunol. 11:594775. PubMed
  164. Spiliopoulou P, et al. 2022. Mol Cancer Ther. . PubMed
  165. Tintos-Hernandez JA, et al. 2021. Brain Commun. 3:fcab215. PubMed
  166. Koutník J, et al. 2022. Cell Commun Signal. 20:54. PubMed
  167. Zhao X, et al. 2022. iScience. 25:104690. PubMed
  168. Cérbulo-Vázquez A, et al. 2022. PLoS One. 17:e0264566. PubMed
  169. Gao J, et al. 2022. Front Immunol. 13:955812. PubMed
  170. Ansari M, et al. 2017. Cell Signal. 10.1016/j.cellsig.2017.04.014. PubMed
  171. Ahmad S, et al. 2017. Prog Neuropsychopharmacol Biol Psychiatry. 10.1016/j.pnpbp.2017.06.034. PubMed
  172. Xia Y, et al. 2018. Cell. 175:1059. PubMed
  173. DiMuzio JM, et al. 2021. Vaccine X. 8:100098. PubMed
  174. Lee G, et al. 2021. Viruses. 13: . PubMed
  175. Lei L, et al. 2021. Front Cell Dev Biol. 659744:9. PubMed
  176. Frafjord A, et al. 2020. Scand J Immunol. e12889:92. PubMed
  177. Lownik JC, et al. 2020. Biochem Biophys Rep. 24:100803. PubMed
  178. Labi V, et al. 2019. Genes Dev. 33:1673. PubMed
  179. Rhoades NS, et al. 2022. Cell Rep. 39:110725. PubMed
  180. Reuschl AK, et al. 2022. Cell Rep. 39:110650. PubMed
  181. Tatangelo V, et al. 2022. Front Oncol. 12:877495. PubMed
  182. Huang T, et al. 2022. Nat Commun. 13:3489. PubMed
  183. Deng Y, et al. 2021. Nat Commun. 12:7041. PubMed
  184. Sun X, et al. 2017. Microbes Infect. 10.1016/j.micinf.2017.08.007. PubMed
  185. Hernandez AA, et al. 2020. J Immunol. 205:2806. PubMed
  186. Su Y, et al. 2020. Cell. 1479:183. PubMed
  187. Wawrzyniak M, et al. 2021. Pharmacol Res Perspect. 9:e00837. PubMed
  188. Mcgaw C, et al. 2022. Nat Commun. 13:2833. PubMed
  189. Wagner AK, et al. 2022. iScience. 25:105137. PubMed
  190. Liu X, et al. 2021. Adv Sci (Weinh). 8:e2100233. PubMed
  191. Heckler M, et al. 2021. Cancer Discov. Online ahead of prin. PubMed
  192. Zheng L, et al. 2020. Clin Cancer Res. 26:3694. PubMed
  193. Witt G, et al. 2020. Cell Biol Toxicol. . PubMed
  194. Marongiu L, et al. 2013. PLoS One. 8:75684. PubMed
  195. Sun Y, et al. 2022. iScience. 25:104846. PubMed
  196. van Loon K, et al. 2022. Cancers (Basel). 14:. PubMed
  197. Wang X, et al. 2022. Elife. 11:. PubMed
  198. Giadone RM, et al. 2018. Amyloid. 25:148. PubMed
  199. Gebremeskel S, et al. 2017. Cancer Immunol Res. 0.9625. PubMed
  200. Zhang Z, et al. 2021. Front Immunol. 12:699478. PubMed
  201. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  202. Chen Y, et al. 2021. Braz J Med Biol Res. 54:e9570. PubMed
  203. Xiao C, et al. 2021. STAR Protoc. 2:100789. PubMed
  204. Luvanda MK, et al. 2021. J Fungi (Basel). 7:. PubMed
  205. Chen S, et al. 2020. Cancer Immunol Res. 1064:8. PubMed
  206. Assadi G, et al. 2016. PLoS One. 11:e0168276. PubMed
  207. Ikebuchi R, et al. 2016. Sci Rep. 6:35002. PubMed
  208. Lei F, et al. 2012. J Vis Exp. 63: 3986. PubMed
  209. Guo Z, et al. 2022. Neurobiol Pain. 12:100096. PubMed

Antigen Details

Antigen References

1. Current Protocols in Immunology (John Wiley & Sons New York) Unit 6.24 Detection of Intracellular Cytokines by Flow Cytometry (Barbara Foster and Calman Prussin NIAID NIH Bethesda MD).
2. Sander B, et al. 1991. Immunol. Rev. 119:65.
3. Sander B, et al. 1993. J. Immunol. Meth. 166:201.
4. Prussin C, et al. 1995. J. Immunol. Meth. 188:117.

Gene ID
NA

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Go To Top Version: 3    Revision Date: 08.26.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

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