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PE anti-CD31 (PECAM-1) Antibody

Pricing & Availability
Clone
JC70 (See other available formats)
Regulatory Status
RUO
Other Names
Platelet and endothelial cell adhesion molecule (PECAM-1), EndoCAM
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
a. JC70_PE_CD31_Antibody_1_070225
Human peripheral blood granulocytes were stained with PE anti-human CD31 (PECAM-1) (clone JC70) (filled histogram) or PE Mouse IgG1, κ Isotype control (open histogram) (Cat. No. 400111). Cells were analyzed for surface flow cytometry.
  • a. JC70_PE_CD31_Antibody_1_070225
    Human peripheral blood granulocytes were stained with PE anti-human CD31 (PECAM-1) (clone JC70) (filled histogram) or PE Mouse IgG1, κ Isotype control (open histogram) (Cat. No. 400111). Cells were analyzed for surface flow cytometry.
  • b. JC70_PE_CD31_Antibody_2_070225
    Human peripheral blood was fixed and permeabilizated using Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) and then intracellular stained with PE anti-human CD31 (PECAM-1) (clone JC70) or PE mouse IgG1, κ Isotype control (open histogram, dashed line) (Cat. No. 400111). Filled histogram represents granulocytes, and open histogram solid line represent lymphocytes.
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623555 25 tests 144€
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623556 100 tests 360€
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Description

CD31, widely known as “platelet and endothelial cell adhesion molecule” (PECAM), is a crucial transmembrane glycoprotein primarily found on the surface of endothelial cells and platelets. The quintessential endothelial cell marker, CD31 is vital for the maintenance of vascular integrity. It plays a pivotal role in cell-cell adhesion, promoting interactions between neighboring endothelial cells, and facilitates leukocyte diapedesis during the inflammatory response. CD31 also mediates platelet-endothelial interactions, contributing to platelet aggregation and thrombus formation at sites of vascular injury. Additionally, this multifunctional molecule is involved in signal transduction pathways that govern cell migration, proliferation, and survival, making it a key player in immune response regulation and angiogenesis. 

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Cell membrane preparation from the spleen of a patient with hairy cell leukemia
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Additional Product Notes

When using this antibody in intracellular flow cytometry (ICFC) on human peripheral blood, we recommend Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803). We do not recommend True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401).

Antigen Details

Structure
CD31 is a 738 amino acid protein with a predicted molecular weight of 82.5 kD.
Distribution

Endothelial cells, Platelets, Granulocytes, Lymphocytes, Monocytes, Neutrophils, Cell surface

Function
Cell adhesion
Interaction
BDKRB2 and GNAQ
Ligand/Receptor
Forms homophillic interactions, also CD38, CD177, αV/β3 integrin
Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
  2. DeLisser HM, et al. 1994. Immunol. Today. 15:490-5.
  3. Newman PJ, et al. 1990. Science 247:1219-22.
  4. Lertkiatmongkol P, et al. 2016. Curr Opin Hematol. 23:253-9.
  5. Yeh JC, et al. 2008. Biochemistry. 47:9029-39.
Gene ID
5175 View all products for this Gene ID
UniProt
View information about CD31 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 07.02.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

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