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Purified anti-FOLR1 (Folate Binding Protein) Antibody (Previously Covance catalog# SIG-3619)

Pricing & Availability
Clone
LK26 (See other available formats)
Regulatory Status
RUO
Other Names
Folate receptor alpha, FR-alpha, KB cells FBP, folate binding protein, folate receptor, adult, adult folate-binding protein, ovarian tumor-associated antigen MOv18
Previously
Signet Catalog# 619-01
Covance Catalog# SIG-3619
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
LK26_Purified_FORL1_Antibody_123118.png
IHC staining of purified anti-FOLR1 (Folate Binding Protein) antibody (clone LK26) on frozen human placenta tissue. The tissue was incubated with a 1:40 dilution of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • LK26_Purified_FORL1_Antibody_123118.png
    IHC staining of purified anti-FOLR1 (Folate Binding Protein) antibody (clone LK26) on frozen human placenta tissue. The tissue was incubated with a 1:40 dilution of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • LK26_Purified_FORL1_Antibody_2_063025
    U251-MG cells (negative cell line, panel A) and KB cells (positive cell line, panel B) were fixed with 4% PFA Fixation Buffer (Cat. No. 420801) for 10 minutes, permeabilized with 100% ice-cold methanol for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with Purified anti-FOLR1 (Folate Binding Protein) (clone LK26), followed by incubation with Alexa Fluor® 594 Goat anti-mouse IgG (Cat. No. 405326) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective and merged. Scale bar: 50 μm
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908301 1 mL 483€
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Description

Folate receptor (FOLR1), also known as folate receptor alpha, is a folate-binding protein (FBP) expressed in adults. It has high affinity for folic acid and its metabolites, including 5-methyltetrahydrofolate (5-MTF), the biologically active form of folic acid used for DNA synthesis and the cysteine cycle. FOLR1 mediates intracellular delivery of 5-MTF. FOLR1 is overexpressed in a variety of tumors, including breast, colorectal, and brain.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was generated against gestational choriocarcinoma cell line, Lu-75 and detects folate-binding protein (FBP).
Formulation
Phosphate-buffered solution with BSA + 0.09% NaN3.

Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Preparation
The antibody was purified by affinity chromatography.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Store between 2°C and 8°C.
Application

IHC-F - Quality tested

ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with any of the following:
-Fixation buffer (Cat. No. 420801) with 0.5 % Triton X-100
-Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol
-100% ice-cold methanol only
Fixation and permeabilization with Fixation buffer followed by Triton X-100 results in dimmer signal compared to the other methods.

Application References

(PubMed link indicates BioLegend citation)
  1. Hviid TV, et al. 1999. Prenat Diagn. 19: 271.
  2. Chesa-Garin P, et al. 1993. Am J Pathol. 142: 557.
  3. Rettig WJ, et al. 1985. Int J Cancer. 35:469.
RRID
AB_2565084 (BioLegend Cat. No. 908301)

Antigen Details

Structure
The LK26 antigen is a 29.8 kD cell surface glycoprotein
Gene ID
2348 View all products for this Gene ID
UniProt
View information about FOLR1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 7    Revision Date: 06.30.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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