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Purified anti-GLI3 Antibody

Pricing & Availability
Clone
W24017B (See other available formats)
Regulatory Status
RUO
Other Names
Transcriptional activator GLI3, Transcriptional repressor GLI3R, GLI3 form of 190 kDa (GLI3-190), GLI3 full-length protein (GLI3FL)
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
A. W24017B_PURE_GLI3_WB_040725A
Whole cell extracts (15 µg total protein) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-GLI3 (clone W24017B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A. W24017B_PURE_GLI3_WB_040725A
    Whole cell extracts (15 µg total protein) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-GLI3 (clone W24017B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • B. W24017B_PURE_GLI3_ICFC_040725
    NTERA-2 cells (positive cell line, filled histogram) and Hep G2 cells (negative cell line, open histogram) were fixed and permeabilized using Cyto-Fast™ Fix/Perm Buffer (Cat. No. 426803) and intracellularly stained with Purified anti-GLI3 (clone W24017B) or Purified Rat IgG2b, κ Isotype Control (open histogram, dashed line) (Cat. No. 400602) followed by Alexa Fluor® 647 Goat anti-rat IgG antibody (Cat. No. 405416).
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647751 25 µg 136€
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647752 100 µg 336€
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Description

GLI3 is a transcription factor in which plays a significant role in embryonic development and cellular differentiation. It belongs to the GLI family of zinc-finger transcription factors, which mediate the Hedgehog (Hh) signaling pathway. One of the key functions of GLI3 is in limb and brain development, where it regulates gene expression patterns crucial for proper morphogenesis. In the absence of Hedgehog signaling, GLI3 is processed into a truncated repressor form (GLI3R), which suppresses the transcription of Hedgehog target genes. In the presence of Hedgehog signaling, GLI3 exists in a full-length activator form (GLI3A), promoting gene expression. This dynamic regulation is essential for processes such as digit specification in limb formation, as GLI3 controls the balance between anterior and posterior limb identities.

Mutations in GLI3 are linked to several congenital disorders, including Greig cephalopolysyndactyly syndrome (GCPS), Pallister-Hall syndrome (PHS), and polydactyly. GLI3 also plays a role in neural tube patterning, contributing to the differentiation of neurons and glial cells which involves other signaling pathways, such as Wnt and BMP, highlighting its integrative role in developmental regulation. Beyond embryogenesis, GLI3 is implicated in cancer progression and tissue homeostasis, as aberrant Hedgehog signaling and GLI3 mutations have been associated with tumorigenesis in basal cell carcinoma and medulloblastoma. 

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human GLI3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 0.5 µg/mL. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

For use of this antibody in intracellular flow cytometry (ICFC), it is recommended to fix/permeabilize with Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) or True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401). Fixation/permeabilization with True-Phos™ Perm Buffer (Cat. No. 425401) is not recommended.

This antibody is not recommended for immunocytochemistry (ICC) or immunohistochemistry in FFPE tissue (IHC-P).

RRID
AB_3699074 (BioLegend Cat. No. 647751)
AB_3699074 (BioLegend Cat. No. 647752)

Antigen Details

Structure
GLI3 is a 1580 amino acid length protein with a predicted molecular weight of 169.9 kD and contains C2H2-type zinc-finger.
Distribution

Located in the nucleus and cytoplasm; Expresses widely in normal adult tissue, including lung, colon, and spleen

Function
As a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway, and plays a role in limb development
Interaction
Interacts with SUFU, KIF3A, KIF7, ZIC1, and BTRC
Cell Type
Astrocytes, Neurons, NK cells, T cells
Biology Area
Transcription Factors
Antigen References
  1. Sasaki H, et al. 1999. Development. 126:3915-24.
  2. Litingtung Y, et al. 2000. Nat Neurosci. 3:979-85.
  3. Miyahara H, et al. 2014. Neuropathology. 34:1-10.
  4. Shen M, et al. 2021. Biomed. Res. Int. 2021:8889986.
Gene ID
2737 View all products for this Gene ID
UniProt
View information about GLI3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04.07.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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