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Purified anti-Keratin 14 Antibody

Pricing & Availability
Clone
W24068C (See other available formats)
Regulatory Status
RUO
Other Names
Keratin, type I cytoskeletal 14 (KRT14), CK-14, cytokeratin 14, keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner)
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
A. W24068C_PURE_Keratin14_IHC-P_070125
IHC staining with Purified anti-Keratin14 (clone W24068C) on formalin-fixed paraffin-embedded human skin tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with primary antibody overnight at 4°C. The next day, tissues were incubated with Biotin Goat anti-rat IgG Antibody (Cat. No. 405428), followed by BioLegend’s Ultra Streptavidin HRP Kit (Multi-Species, Cat. No. 929501) and AEC Chromogen Concentrate (Cat. No. 925804) was used for detection followed by hematoxylin counterstaining. Images were captured on a Keyence microscope with a 40X objective. Scale bar: 50 µm
  • A. W24068C_PURE_Keratin14_IHC-P_070125
    IHC staining with Purified anti-Keratin14 (clone W24068C) on formalin-fixed paraffin-embedded human skin tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with primary antibody overnight at 4°C. The next day, tissues were incubated with Biotin Goat anti-rat IgG Antibody (Cat. No. 405428), followed by BioLegend’s Ultra Streptavidin HRP Kit (Multi-Species, Cat. No. 929501) and AEC Chromogen Concentrate (Cat. No. 925804) was used for detection followed by hematoxylin counterstaining. Images were captured on a Keyence microscope with a 40X objective. Scale bar: 50 µm
  • B. W24068C_PURE_Keratin14_ICC_070125
    U-87 MG cells (panel A, negative cell line) and HaCaT cells (panel B, positive cell line) were fixed with 4% PFA Fixation Buffer (Cat. No. 420801) for 10 minutes and permeabilized with 100% ice-cold methanol for 10 minutes and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with Purified anti-Keratin 14 (clone W24068C), followed by incubation with Alexa Fluor® 594 Goat anti-rat IgG (Cat. No. 405422) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective and merged. Scale bar: 50 µm
  • C. W24068C_PURE_Keratin14_WB_070125
    Whole cell extracts (15 µg total protein per lane) from the indicated cell lines resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-Keratin 14 (clone W24068C) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker
  • D. 24068C_PURE_Keratin14_ICFC_070125
    HaCaT cells (positive cell line, filled histogram) and U-87 MG cells (negative cell line, open histogram) were fixed and permeabilized using True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) and intracellularly stained with Pure anti-Keratin 14 (clone W24068C), or Purified Rat IgG2b, κ Isotype Control (open histogram, dashed line, representative of both the positive and negative cell lines) (clone RTK4530) (Cat. No. 400601) followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416).
Cat # Size Price Quantity Check Availability Save
648814 25 µg 124€
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648815 100 µg 336€
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Description

Keratin 14 is a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant peptide corresponding to the C terminus of human keratin 14
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
ICC, WB, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg/mL is recommended. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704).

For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with any of the following:
-Fixation buffer (Cat. No. 420801) with 0.5 % Triton X-100
-Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol
-100% ice-cold methanol only

For use in intracellular flow cytometry (ICFC), it is recommended to fix/permeabilize with any of the following:
-Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803)
-True-Phos™ Perm Buffer (Cat. No. 425401)
-True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) 
-True-Nuclear™ Transcription Factor Buffer Set gives the most robust signal.

This product does not cross-react with mouse keratin 14 by Western Blot.

Antigen Details

Structure
Keratin 14 is a 472 amino acid protein with a molecular mass of 52 kD.
Distribution

Tissue distribution: skin
Cellular distribution: cytoplasm, nucleus

Function
Nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles, enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.
Interaction
Keratin 5, desmosomal proteins, plectin
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Antigen References
  1. Wend P, et al. 2013. EMBO J. 32:1977-89.
  2. Welm AL, et al. 2005. Proc Natl Acad Sci USA. 102:4324-9
  3. Hu Y, et al. 2001. Nature. 410:710-714.
  4. Yuspa SH, et al. 1989. J Cell Biol. 109:1207-1217.
  5. Roop DR, et al. 1984. J Biol Chem. 259:8037-8040.
Gene ID
3861 View all products for this Gene ID
UniProt
View information about Keratin 14 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07.01.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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