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Purified anti-MRP-14 (S100A9) Antibody

Pricing & Availability
Clone
A22135C (See other available formats)
Regulatory Status
RUO
Other Names
S100A9, Migration inhibitory factor-related proteins-14, Calgranulin-B, Leukocyte L1 complex heavy chain, Calprotectin L1H subunit
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A. A22135C_PURE_MRP-14_IHC-P_072325
IHC staining with Purified anti-MRP-14 (S100A9) (clone A22135C) on formalin-fixed paraffin-embedded human esophagus tissue. Following antigen retrieval using 1X Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704), the tissue was incubated without (panel A) or with (panel B) Purified anti-MRP-14 (S100A9) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured using with a 40X objective and merged. Scale bar: 50 µm
  • A. A22135C_PURE_MRP-14_IHC-P_072325
    IHC staining with Purified anti-MRP-14 (S100A9) (clone A22135C) on formalin-fixed paraffin-embedded human esophagus tissue. Following antigen retrieval using 1X Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704), the tissue was incubated without (panel A) or with (panel B) Purified anti-MRP-14 (S100A9) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured using with a 40X objective and merged. Scale bar: 50 µm
  • B. A22135C_PURE_MRP-14_ICC_072325
    Lysed human whole blood was fixed with Fixation Buffer (Cat. No. 420801) for 10 minutes, permeabilized with 100% ice-cold methanol for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with Purified anti-MRP-14 (S100A9) (clone A22135C), followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective and merged. Scale bar: 50 µm
  • C. A22135C_PURE_MRP-14_ICFC_072325
    Lysed human whole blood gated on granulocyte population (positive expression, filled histogram) and lymphocyte population (negative expression, open histogram) were fixed and permeabilized using Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) and intracellularly stained with Purified anti-MRP-14 (S100A9) (clone A22135C), or Purified Mouse IgG2b, κ Isotype Control (Cat. No. 400301) (dashed histogram, representative of both the positive and negative cell populations) followed by incubation with PE Goat anti-mouse IgG (Cat. No. 405307).
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648820 25 µg 128€
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648821 100 µg 328€
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Description

The S100A8/A9 complex has been shown to play a role in calcium-dependent activation and exhibits antibacterial, antifungal, immunomodulatory, and anti-proliferative effects. It also functions as a chemoattractant for neutrophils and serves as an endogenous activator of Toll-like receptor 4 (TLR4). S100A9 has been identified as a marker of CD14⁺ HLA-DR⁻/low myeloid-derived suppressor cells (MDSCs).

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant protein of human MRP-14 (S100A9)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
ICC, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 10.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704).

For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol.

For use in intracellular flow cytometry (ICFC), it is recommended to fix/permeabilize with Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803). Fixation/permeabilization with either True-Phos™ Perm Buffer (Cat. No. 425401) or True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) is not recommended.

Antigen Details

Structure
MRP-14 (S100A9) s is a 114 amino acid protein with a predicted molecular weight of 13.2 kD.
Distribution

Secreted and localized to the cytoplasm and cell membrane in cells such as MDSCs, neutrophils, monocyte, and ileal tissue eosinophil.

Function
Calcium-dependent activation, immunomodulating and antiproliferative, endogenous activator of Toll-like receptor 4
Cell Type
Eosinophils, Monocytes, Neutrophils
Biology Area
Cancer Biomarkers, Cell Biology, Immunology, Innate Immunity
Antigen References
  1. Loser K, et al. 2010. Nat. Med. 16:713.
  2. Cheng P, et al. 2008. J. Exp. Med. 205:2235.
  3. Leukert N, et al. 2006. J. Mol. Biol. 359:961.
  4. Lagasse E, et al. 1988. Mol. Cell. Biol. 8:2402.
  5. Zhao F, et al. 2012. Immunology. 136:176.
Gene ID
6280 View all products for this Gene ID
UniProt
View information about MRP-14 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07.23.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

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