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Purified anti-PIAS4 Antibody

Pricing & Availability
Clone
W22136A (See other available formats)
Regulatory Status
RUO
Other Names
E3 SUMO-protein ligase PIAS4, PIASG, PIASY, ZMIZ6, Protein Inhibitor Of Activated STAT Protein Gamma, Protein Inhibitor Of Activated STAT Protein 4, FLJ12419
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W22136A_PURE_-PIAS4_WB_031725
Whole cell extracts (15 µg total protein per lane) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-PIAS4 (clone W22136A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • W22136A_PURE_-PIAS4_WB_031725
    Whole cell extracts (15 µg total protein per lane) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-PIAS4 (clone W22136A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
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672251 25 µg 140€
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672252 100 µg 364€
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Description

PIAS4 (Protein Inhibitor of Activated STAT protein 4), also known as E3 SUMO-protein ligase PIAS4, is a multifunctional protein that regulates transcription and is essential in various cellular processes. PIAS4 facilitates the attachment of SUMO (Small Ubiquitin-like Modifier) to target proteins and modulates protein stability, localization, and interactions. PIAS4 regulates the activity of transcription factors such as STATs (Signal Transducers and Activators of Transcription) by promoting their SUMOylation. PIAS4 participates in the DNA damage response, where it facilitates the recruitment of repair factors like 53BP1 to sites of DNA damage. This SUMOylation-driven process is vital for preserving genomic stability, preventing mutations, and reducing the risk of tumorigenesis. PIAS4 also influences chromatin dynamics by interacting with chromatin-associated proteins, affecting chromatin structure and gene expression patterns. PIAS4 is also crucial in immune signaling, particularly in pathways like interferon signaling and NF-κB activation. By SUMOylating transcription factors and signaling molecules, PIAS4 fine-tunes the immune response, balancing pro-inflammatory and anti-inflammatory signals. Dysregulation of PIAS4 has been implicated in various diseases, particularly cancer and inflammatory disorders.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human PIAS4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is not recommended for immunocytochemistry (ICC) or intracellular flow cytometry (ICFC).

RRID
AB_3699088 (BioLegend Cat. No. 672251)
AB_3699088 (BioLegend Cat. No. 672252)

Antigen Details

Structure
PIAS4 is a 510 amino acid length protein with a predicted molecular weight of 56.5 kD and contains LXXLL motif.
Distribution

Localized in the nucleus

Function
Mediates sumoylation and co-regulates transcriptional activity with STAT1 and TP53
Interaction
Interacts with GATA2, LEF1, TP53 and STAT1
Cell Type
Leukocytes
Biology Area
Cell Cycle/DNA Replication, DNA Repair/Replication, Transcription Factors, Ubiquitin/Protein Degradation
Molecular Family
Nuclear Markers, Protein Modifiers
Antigen References
  1. Galanty Y, et al. 2009. Nature. 462:935-9.
  2. Wei J, et al. 2011. J Natl. Cancer Inst. 103:1552-6.
  3. Mohiuddin M, et al. 2018. Proc Natl Acad Sci USA. 115:12793-12798.
  4. Yan Y, et al. 2023. 21:e3002379.
Gene ID
51588 View all products for this Gene ID
UniProt
View information about PIAS4 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 03.18.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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