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Purified anti-TMEM97 Antibody

Pricing & Availability
Clone
W22180E (See other available formats)
Regulatory Status
RUO
Other Names
MAC30, Meningioma-associated protein 30; σ2R; Sigma intracellular receptor 2, Sigma-2 receptor, Transmembrane protein 97, S2R
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A. W22180E_PURE_-TMEM97_WB_61925
Whole cell extracts (15 µg total protein per lane) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with Purified anti-TMEM97 (clone W22180E) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:5000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A. W22180E_PURE_-TMEM97_WB_61925
    Whole cell extracts (15 µg total protein per lane) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with Purified anti-TMEM97 (clone W22180E) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:5000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • B. W22180E_PURE_-TMEM97_IHC-P-1_61925
    IHC staining with Purified anti-TMEM97 (clone W22180E) was performed on FFPE human pancreas tissue following antigen retrieval using 1X Citrate buffer (pH 6.0) (Cat. No. 420902). The tissue sections were incubated with Purified anti-TMEM97 (clone W22180E) (panel B and merged in panel C) followed by Alexa Fluor® 594 Goat anti-rat (Cat. No. 405422) (red) or secondary antibody only (negative control, panel A). Nuclei were counterstained with DAPI (Cat. No. 422801) (blue) (panels A and C). The image was captured wit a 40X objective. Scale Bar: 50 µm
  • C. W22180E_PURE_-TMEM97_IHC-P-2_61925
    IHC staining with Purified anti-TMEM97 (clone W22180E) was performed on FFPE human testis (panel A) and brain tissue (panel B) following antigen retrieval using 1X Citrate buffer (pH 6.0) (Cat. No. 420902). The tissue sections were incubated with 5 µg/mL of Purified anti-TMEM97 (clone W22180E), followed by Alexa Fluor® 594 Goat anti-rat (Cat. No. 405422) (red). Nuclei were counterstained with DAPI (Cat. No. 422801) (blue). The image was captured wit a 40X objective. Scale Bar: 50 µm
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656951 25 µg 144€
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656952 100 µg 376€
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Description

Sigma intracellular receptor 2 (σ2R), also known as SIGMAR2 or TMEM97, is a transmembrane protein that is associated with the endoplasmic reticulum (ER), membranes and lysosomes. It is widely distributed in normal tissues such as the brain, lungs, heart, skeletal muscles, testis, and ovary. TMEM97 has been demonstrated to regulate the cholesterol transporter NPC1 in maintaining cholesterol homeostasis. Elevated expression of TMEM97 is a potential prognostic biomarker for breast, lung, colon, ovarian, bladder and oral squamous cancers. TMEM97 is also implicated in several neurological disorders and age-related diseases such as Alzheimer's disease.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human TMEM97 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

For use in immunohistochemistry on FFPE tissues (IHC-P), antigen retrieval with Citrate Buffer (Cat. No. 420901) is recommended.

This antibody (clone W22180E) does not cross-react with mouse TMEM97 in Western Blot.

We do not recommend this antibody for use in immunocytochemistry (ICC).

Antigen Details

Structure
TMEM97 is a 176 amino acid protein with a predicted molecular weight of 21 kD.
Distribution

Widely expressed in normal tissues with increased expression in proliferating cancer cells.

Function
Controlling cellular cholesterol levels, cancer biomarker, modulates the cytosolic Ca2+ concentration, dopaminergic transmission, and cocaine-induced addiction behavior.
Interaction
Interacts with NPC1, PGRMC1, LDLR, histatin 1/HTN1 and TSPO.
Cell Type
Microglia, Neurons
Biology Area
Cell Biology, Neurodegeneration, Neuroscience
Molecular Family
GPCR
Antigen References
  1. Bartz, F. et al. 2009. Cell Metab. 10:63.
  2. Narayanan S, et al. 2011. Future Med Chem. 3:79.
  3. Oyer HM, et al. 2019. Front Pharmacol. 10:1141.
  4. Yi B, et al. 2017. J Neurochem. 140:561.
  5. Vazquez-Rosa E, et al. 2019. ACS Chem Neurosci.10:1595.
  6. Riad, A. et al. 2020. Mol Neurobiol. 57:3803.
Gene ID
27346 View all products for this Gene ID
UniProt
View information about TMEM97 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06.19.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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