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- Regulatory Status
- RUO
- Other Names
- Interleukin-1 beta, IL-1 beta, IL1B, IL1F2, Catabolin
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Recombinant human IL-1β precursor (2 µg/lane) was separated by SDS-PAGE under reduced and non-reduced conditions and stained with Coomassie brilliant blue dye (A). Lane M: Protein ladder (Cat No. 773302). -
Recombinant human IL-1β precursor was coated in serial dilution to perform indirect ELISA (B) using a biotinylated secondary antibody (Cat. No. 405428).
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 714254 | 25 µg | 278€ | ||||
Interleukin-1 (IL-1) is a proinflammatory cytokine and a central mediator of innate immunity and inflammation. IL-1β is produced as a precursor called pro-IL-1β mainly by inflammatory cells of myeloid lineage such as such as blood monocytes, tissue macrophages, skin dendritic cells and brain microglia. Unlike the IL-1α precursor, the IL-1β precursor or the proform is not active. Pro-IL-1β is rapidly induced upon exposure of inflammatory cells to pathogen-associated molecular patterns (PAMPs) or damage-associated molecular patterns (DAMPs) that bind to pattern recognition receptors (PRRs) to upregulate proinflammatory gene expression. Following activation, inflammasomes cause the activation of caspase-1 and the processing of pro-IL-1β to a mature secretory form. Extracellular cleavage of the inactive IL-1β precursor by neutrophil enzymes such a proteinase-3 and elastase generate active IL-1β because the cleavage site is close to that of caspase-1. IL-1β does not traffic through the ER or Golgi and the precise mechanisms of its secretion are poorly defined. Potential secretory mechanisms include its release through the regulated secretion of lysosomes, the shedding of microvesicles from the plasma membrane, or its direct release across a hyper-permeable plasma membrane. Although more typically associated with classical inflammation, IL-1β has also been implicated in angiogenic activity of established mouse tumor cell lines and very recently, in angiogenesis and invasion in human solid tumors. While IL-1β can directly affect the survival and proliferation of endothelial cells, it can also promote induction of other proangiogenic factors such as TNFα, angiopoietin-1, IL-6 and VEGF A.
Product DetailsProduct Details
- Source
- Human IL-1β precursor, amino acids Met1-Ser269 (Accession # P01584), with a N-terminal His tag, was expressed in E. Coli.
- Molecular Mass
- The 296 amino acid recombinant protein has a predicted molecular mass of approximately 33.7 kD. The DTT-reduced and non-reduced protein migrate at approximately 35-40 kD in SDS-PAGE.
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N-terminal
Sequence Analysis - The predicted N-terminal amino acid is Met.
- Purity
- > 85%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS, pH 7.2
- Endotoxin Level
- Less than 1.0 EU per μg of cytokine as determined by the LAL method
- Concentration
- 25 µg at 0.2 mg/mL (one size only)
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at - 20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 μg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated. freeze/thaw cycles
- Application
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Standard
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Monomer
- Distribution
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Blood monocytes, tissue macrophages, skin dendritic cells and brain microglia
- Function
- Upregulation of proinflammatory gene expression, survival and proliferation of endothelial cells, induction of proangiogenic factors such as TNFα, angiopoietin-1, IL-6 and VEGF A
- Interaction
- Integrins; cargo receptor TMED10, HSP90B1/AB1
- Ligand/Receptor
- Type I and II IL-1 receptors (IL-1RI and IL-1RII)
- Cell Type
- Hematopoietic stem and progenitors
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Stem Cells
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
- Garlanda C, et al. 2013. Immunity. 39:1003-1018.
- Takeuchi O and Akira S. 2010. Cell. 140:805-820.
- Dinarello CA. 2011. Blood. 117:3720-3732.
- Kayagaki N, et al. 2011. Nature. 479:117-121.
- Andrei C, et al. 2004. Proc. Natl. Acad. Sci. 101:9745-9750.
- MacKenzie A, et al. 2001. Immunity. 15:825-835.
- Shirasaki Y, et al. 2014. Sci Rep. 4:4736.
- Voronov E, et al. 2003. Proc. Natl. Acad. Sci. 100:2645-2650.
- Elaraj DM, et al. 2006. Clin. Cancer Res. 12:1088-1096.
- Carmi Y, et al. 2013. J. Immunol. 190:3500-3509.
- Fan F, et al. 2004. Cancer Res. 64:3186-3190.
- Alagappan VK, et al. 2005. Cell Biochem. Biophys. 43:119-129.
- Stocks J, et al. 2005. FEBS Lett. 579:2551-2556.
- Gene ID
- 3553 View all products for this Gene ID
- UniProt
- View information about IL-1beta on UniProt.org
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
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We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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