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StarBright UltraViolet 740 anti-human CD366 (Tim-3) Antibody

Pricing & Availability
Clone
A18087E (See other available formats)
Regulatory Status
RUO
Other Names
T cell immunoglobulin and mucin domain containing protein 3, hepatitis virus cellular receptor 2, CD366
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A. A18087E_SBUV740_CD366-_FC_1_071125
Human peripheral blood cells were stained with anti-human CD3 (clone UCHT1) FITC and anti-human CD366 (Tim-3) (clone A18087E) StarBright UltraViolet 740 (left) or stained with anti-human CD3 (clone UCHT1) FITC only (right). Data shown are gated on the lymphocyte population.
  • A. A18087E_SBUV740_CD366-_FC_1_071125
    Human peripheral blood cells were stained with anti-human CD3 (clone UCHT1) FITC and anti-human CD366 (Tim-3) (clone A18087E) StarBright UltraViolet 740 (left) or stained with anti-human CD3 (clone UCHT1) FITC only (right). Data shown are gated on the lymphocyte population.
  • B. A18087E_SBUV740_CD366_FC_2_060525
    PHA-stimulated (3 days) human peripheral blood cells were stained with anti-human CD366 (Tim-3) (clone A18087E) StarBright UltraViolet 740 (filled histogram). Open histogram represents unstained cells. Data shown are gated on the lymphocyte population.
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364822 100 tests 300€
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This StarBright conjugate is available for purchase as part of a multicolor flow cytometry panel. To order, add compatible products to your shopping cart, or use our Panel Selector tool to build your ideal panel. For ordering assistance or to request a sample, contact your local field representative.

This product is also available for direct purchase within a StarBright Basic Panel, which combines a minimal set of pre-selected compatible reagents.

Description

CD366 (Tim-3) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-3. Tim-3 is expressed at high levels on activated T cells (preferentially on Th1 cells, monocytes/macrophages, and dendritic cells). Tim-3 has also been shown to exist as a soluble protein. Cells expressing Tim-3 are present at high levels in the CNS of animals at the onset of experimental autoimmune encephalomyelitis (EAE), a disease mediated by lymphocytes secreting Th1-like cytokines. Tim-3 has been proposed to inhibit Th1-mediated immune responses and promote immunological tolerance.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human Tim-3
Formulation
Phosphate-buffered saline, containing 0.09% sodium azide, 0.05% Tween 20, 0.1% PEG 3350, 0.1% Pluronic F68, and 1% BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with StarBright UltraViolet 740 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) The concentration relates to the dye component of the conjugate.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* StarBright UltraViolet 740 has a maximum excitation of 343 nm and a maximum emission of 737 nm.


StarBright dyes are powered by Bio-Rad®. STARBRIGHT is a trademark of Bio-Rad Laboratories, Inc.
Excitation Laser
Ultraviolet Laser (355 nm)

Antigen Details

Structure
Transmembrane protein containing immunoglobulin domain and mucin-like domain; can exist as a soluble form lacking mucin and transmembrane domains
Distribution

Activated T cells, preferentially on Th1 cells, monocytes, dendritic cells

Function
CD366 plays a role in regulating macrophage activation, checkpoint inhibitor, T cell apoptosis and immune tolerance
Ligand/Receptor
Galectin-9 and PtdSer
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References
  1. Hafler DA and Kuchroo V. 2008. J Exp Med. 205:2699.
  2. Zhu C, et al. 2005. Nat Immunol. 6:1245.
  3. Wang F, et al. 2009. Immunobiology. 214:342.
Gene ID
84868 View all products for this Gene ID
UniProt
View information about CD366 on UniProt.org

Related FAQs

What is a “StarBright panel”?

A StarBright panel is a multiplex panel that includes at least one StarBright conjugate.

Will my StarBright panel be provided as a cocktail?

StarBright products purchased as part of a panel or StarBright Basic Panel will be provided in individual vials. To order premade cocktails, please contact our Custom Solutions Team.

Are StarBright GMP/ASR products available?

StarBright ASR and GMP products are coming soon.

Are StarBright custom products available?

Yes, please contact our Custom Solutions Team.

Will StarBright isotype controls be offered?

We are not planning to offer StarBright isotype controls at this time. Fluorescence Minus One (FMO) controls should be used to define gating boundaries.

What amount of a StarBright conjugate should I use?

Antibody conjugates should be titrated for optimal performance in each application. To directly compare performance with a traditional or polymer-conjugated antibody, we recommend using the same volume of each product for analysis. Due to differences in the quantification of product mass, StarBright Dyes are prepared in equivalent volumes.

Why shouldn’t I compare the mass (μg) of a StarBright conjugate to a traditional or polymer conjugate?

For StarBright conjugates, the reported mass refers to the mass of the dye component. For traditional conjugates, the reported mass refers to the mass of the antibody component. This is because traditional conjugates have multiple dye molecules attached to one antibody, while StarBright conjugates have multiple antibodies attached to a single StarBright Dye.

Go To Top Version: 1    Revision Date: 06.05.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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