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APC anti-mouse CD206 (MMR) Antibody APC anti-mouse CD206 (MMR) Antibody

Pricing & Availability
Clone
C068C2 (See other available formats)
Regulatory Status
RUO
Other Names
MMR (macrophage mannose receptor), MR (mannose receptor), MRC1
Isotype
Rat IgG2a, κ
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Product Citations
publications
C068C2_APC_1_082911
Thioglycollate-elicited BALB/c peritoneal macrophages were surface stained with CD107b (Mac-3) PE, and then intracellularly stained with CD206 (clone C068C2) APC (top) or rat IgG2a, κ APC isotype control (bottom).
  • C068C2_APC_1_082911
    Thioglycollate-elicited BALB/c peritoneal macrophages were surface stained with CD107b (Mac-3) PE, and then intracellularly stained with CD206 (clone C068C2) APC (top) or rat IgG2a, κ APC isotype control (bottom).
  • C068C2_APC_2_082911
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141707 25 µg DKK779
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141708 100 µg DKK1759
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Description

CD206, also known as mannose receptor (MR), is a 175 kD type I membrane protein. It is a pattern recognition receptor (PRR) belonging to the C-type lectin superfamily. MR is expressed on macrophages, dendritic cells, Langerhans cells, and hepatic or lymphatic endothelial cells. MR recognizes a range of microbial carbohydrates bearing mannose, fucose, or N-acetyl glucosamine through its C-type lectin-like carbohydrate recognition domains, sulfated carbohydrate antigens through its cysteine-rich domain, and collagens through its fibronectin type II domain. MR mediates endocytosis and phagocytosis as well as activation of macrophages and antigen presentation. It plays an important role in host defense and provides a link between innate and adaptive immunity. Recently, MR on lymphatic endothelial cells was found to be involved in leukocyte trafficking and a contributor to the metastatic behavior of cancer cells. It suggests that MR may be a potential target in controlling inflammation and cancer metastasis by targeting the lymphatic vasculature.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CD206 (MMR)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
FC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone C068C2 recognizes a region similar to clone MR5D3, based on the ability of the clones to block each other. Additional reported applications (for the relevant formats) include: spatial biology (IBEX)4,5.

Application References
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  287. Fujimoto N, et al. 2020. PLoS Biol. 18:e3000704. PubMed
  288. Remmerie A, et al. 2020. Immunity. 53:641. PubMed
  289. Quenum Zangbede FO, et al. 2018. J Neurosci. 38:6737. PubMed
  290. Wei Z, et al. 2021. Nat Commun. 0.805555556. PubMed
  291. Ali S, et al. 2021. PLoS One. 16:e0246646. PubMed
  292. Trott DW, et al. 2021. J Physiol. 599:3973. PubMed
  293. Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed
  294. Guo D, et al. 2022. Bone Res. 10:45. PubMed
  295. Yuan M, et al. 2022. Oxid Med Cell Longev. 2022:5479491. PubMed
  296. Li ZL, et al. 2021. Mol Ther Nucleic Acids. 25:502. PubMed
  297. Dong R, et al. 2021. Frontiers in Immunology. 12:620238. PubMed
  298. Zhang Y, et al. 2020. Mol Med Rep. 22:534. PubMed
  299. Kiyohara H, et al. 2018. Cell Mol Gastroenterol Hepatol. 7:135. PubMed
  300. Settelmeier S, et al. 2020. PLoS One. 15:e0233261. PubMed
  301. He H, et al. 2012. Blood. 120:3152. PubMed
  302. Dai K, et al. 2015. J Leukoc Biol. 98: 1071 - 1080. PubMed
  303. Gao Z, et al. 2022. J Inflamm Res. 15:3337. PubMed
  304. Tomimatsu M, et al. 2022. Sci Rep. 12:16656. PubMed
  305. Rao L, et al. 2020. Nat Commun. 11:4909. PubMed
  306. Chen Y, et al. 2022. Nat Commun. 13:4468. PubMed
  307. Nam J, et al. 2021. Adv Ther (Weinh). 4:. PubMed
  308. Canedo T, et al. 2021. Neuropsychopharmacology. 46:2358. PubMed
  309. Zhang W, et al. 2021. Front Immunol. 12:768435. PubMed
  310. Roux C, et al. 2021. Antioxidants (Basel). 10:. PubMed
  311. Li K, et al. 2020. J Cell Mol Med. 24:13115. PubMed
  312. Huo M, et al. 2017. FASEB J. 10.1096/fj.201601030R. PubMed
  313. Moufarrij S, et al. 2020. Sci Rep. 10:3470. PubMed
  314. Lee SH, et al. 2018. J Exp Med. 215:357. PubMed
  315. Tsunekuni K, et al. 2020. J Clin Med. 9:00. PubMed
  316. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  317. Fang W, et al. 2021. J Exp Clin Cancer Res. 40:4. PubMed
  318. Tang PC, et al. 2022. Adv Sci (Weinh). 9:e2101235. PubMed
  319. Cui Y, et al. 2022. Front Oncol. 12:732860. PubMed
  320. Piirainen S, et al. 2021. Glia. 69:2459. PubMed
  321. Catrysse L, et al. 2021. Cell Rep. 36:109748. PubMed
  322. Zhu Y, et al. 2021. Int J Oral Sci. 13:25. PubMed
  323. Filtjens J, et al. 2021. Nat Commun. 12:2936. PubMed
  324. Siolas D, et al. 2021. Cell Reports. 36:109578. PubMed
  325. Canan C, et al. 2014. J Leukoc Biol. 96:473. PubMed
  326. Lee C, et al. 2020. Front Immunol. 11:77. PubMed
  327. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  328. Baardman J et al. 2018. Cell reports. 25(8):2044-2052 . PubMed
  329. Sadtler K, et al. 2017. Tissue Eng Part A. 23:1044. PubMed
  330. Durga Devi T, et al. 2017. Am J Pathol. 187:2659. PubMed
  331. Kurelac I, et al. 2019. J Clin Med. 1.85. PubMed
  332. Crowe J, et al. 2020. PLoS Pathog. 16:e1008391. PubMed
  333. Song HY, et al. 2021. Mol Ther. S1525-0016:00472-X. PubMed
  334. Luo ZW, et al. 2021. Int J Nanomedicine. 16:2949. PubMed
  335. Chithanathan K, et al. 2022. Biology (Basel). 11:. PubMed
  336. Ding T, et al. 2021. J Nanobiotechnology. 19:247. PubMed
  337. Andreata F, et al. 2021. STAR Protoc. 2:100831. PubMed
  338. Liu Y, et al. 2019. Am J Physiol Cell Physiol. 317:C762. PubMed
  339. Syed I et al. 2018. Cell metabolism. 27(2):419-427 . PubMed
  340. Nasti A, et al. 2017. Eur J Immunol. 47:2163. PubMed
RRID
AB_10896057 (BioLegend Cat. No. 141707)
AB_10900231 (BioLegend Cat. No. 141708)

Antigen Details

Structure
Type I transmembrane protein, 175 kD, C-type lectin superfamily
Distribution

Macrophages, dendritic cells, Langerhans cells, liver endothelial cells

Function
Pathogen recognition, endocytosis and phagocytosis, antigen presentation
Ligand/Receptor
Antigen containing mannose, fucose, or an N-acetyl glucosamine
Cell Type
Dendritic cells, Endothelial cells, Langerhans cells, Macrophages
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
CD Molecules
Antigen References

1. Wileman TE, et al. 1986. P. Natl. Acad. Sci. USA 83:2501.
2. Apostolopoulos V, et al. 2001. Curr. Mol. Med. 1:469.
3. Burgdorf S, et al. 2006. J. Immunol. 176:6770.
4. McKenzie EJ, et al. 2007. J. Immunol. 178:4975.

Gene ID
17533 View all products for this Gene ID
UniProt
View information about CD206 on UniProt.org

Related FAQs

Why is mouse CD206 stained intracellularly and not via surface staining?

Typically, mouse CD206 surface level is relatively low under normal conditions and so intracellular staining protocol is required to get better signal.

Other Formats

View All CD206 Reagents Request Custom Conjugation
Description Clone Applications
Brilliant Violet 711™ anti-mouse CD206 (MMR) C068C2 ICFC,FC
Brilliant Violet 785™ anti-mouse CD206 (MMR) C068C2 ICFC,FC
PE/Dazzle™ 594 anti-mouse CD206 (MMR) C068C2 ICFC,FC
Alexa Fluor® 700 anti-mouse CD206 (MMR) C068C2 ICFC,FC
Spark YG™ 570 anti-mouse CD206 (MMR) C068C2 IHC-F
Biotin anti-mouse CD206 (MMR) C068C2 ICFC,FC
Purified anti-mouse CD206 (MMR) C068C2 ICFC,FC
FITC anti-mouse CD206 (MMR) C068C2 ICFC,FC
PE anti-mouse CD206 (MMR) C068C2 ICFC,FC
APC anti-mouse CD206 (MMR) C068C2 ICFC,FC
Alexa Fluor® 488 anti-mouse CD206 (MMR) C068C2 ICFC,FC,IHC-F
Alexa Fluor® 647 anti-mouse CD206 (MMR) C068C2 ICFC,FC,IHC-F
PerCP/Cyanine5.5 anti-mouse CD206 (MMR) C068C2 ICFC,FC
PE/Cyanine7 anti-mouse CD206 (MMR) C068C2 ICFC,FC
Brilliant Violet 421™ anti-mouse CD206 (MMR) C068C2 ICFC,FC,IHC-F,SB
Brilliant Violet 605™ anti-mouse CD206 (MMR) C068C2 ICFC,FC
Brilliant Violet 650™ anti-mouse CD206 (MMR) C068C2 ICFC,FC
Alexa Fluor® 594 anti-mouse CD206 (MMR) C068C2 IHC-F
PE/Cyanine5 anti-mouse CD206 (MMR) C068C2 ICFC,FC
PE/Fire™ 700 anti-mouse CD206 (MMR) C068C2 ICFC,FC
TotalSeq™-B0173 anti-mouse CD206 (MMR) C068C2 ICPG
Spark Red™ 718 anti-mouse CD206 (MMR) (Flexi-Fluor™) C068C2 FC
Spark Blue™ 574 anti-mouse CD206 (MMR) (Flexi-Fluor™) C068C2 FC
TotalSeq™-C0173 anti-mouse CD206 (MMR) Antibody C068C2 ICPG
Spark YG™ 581 anti-mouse CD206 (MMR) (Flexi-Fluor™) C068C2 FC
Spark YG™ 593 anti-mouse CD206 (MMR) (Flexi-Fluor™) Antibody C068C2 FC
Spark NIR™ 685 anti-mouse CD206 (MMR) (Flexi-Fluor™) Antibody C068C2 FC
Go To Top Version: 3    Revision Date: 04/04/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Pricing & Availability
Clone
C068C2 (See other available formats)
Regulatory Status
RUO
Other Names
MMR (macrophage mannose receptor), MR (mannose receptor), MRC1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
C068C2_APC_1_082911
Thioglycollate-elicited BALB/c peritoneal macrophages were surface stained with CD107b (Mac-3) PE, and then intracellularly stained with CD206 (clone C068C2) APC (top) or rat IgG2a, κ APC isotype control (bottom).
  • C068C2_APC_1_082911
    Thioglycollate-elicited BALB/c peritoneal macrophages were surface stained with CD107b (Mac-3) PE, and then intracellularly stained with CD206 (clone C068C2) APC (top) or rat IgG2a, κ APC isotype control (bottom).
  • C068C2_APC_2_082911
Compare all formats See APC spectral data
Cat # Size Price Quantity Check Availability Save
141707 25 µg DKK779
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141708 100 µg DKK1759
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Description

CD206, also known as mannose receptor (MR), is a 175 kD type I membrane protein. It is a pattern recognition receptor (PRR) belonging to the C-type lectin superfamily. MR is expressed on macrophages, dendritic cells, Langerhans cells, and hepatic or lymphatic endothelial cells. MR recognizes a range of microbial carbohydrates bearing mannose, fucose, or N-acetyl glucosamine through its C-type lectin-like carbohydrate recognition domains, sulfated carbohydrate antigens through its cysteine-rich domain, and collagens through its fibronectin type II domain. MR mediates endocytosis and phagocytosis as well as activation of macrophages and antigen presentation. It plays an important role in host defense and provides a link between innate and adaptive immunity. Recently, MR on lymphatic endothelial cells was found to be involved in leukocyte trafficking and a contributor to the metastatic behavior of cancer cells. It suggests that MR may be a potential target in controlling inflammation and cancer metastasis by targeting the lymphatic vasculature.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CD206 (MMR)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
FC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone C068C2 recognizes a region similar to clone MR5D3, based on the ability of the clones to block each other. Additional reported applications (for the relevant formats) include: spatial biology (IBEX)4,5.

Application References
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  4. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  5. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
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RRID
AB_10896057 (BioLegend Cat. No. 141707)
AB_10900231 (BioLegend Cat. No. 141708)

Antigen Details

Structure
Type I transmembrane protein, 175 kD, C-type lectin superfamily
Distribution

Macrophages, dendritic cells, Langerhans cells, liver endothelial cells

Function
Pathogen recognition, endocytosis and phagocytosis, antigen presentation
Ligand/Receptor
Antigen containing mannose, fucose, or an N-acetyl glucosamine
Cell Type
Dendritic cells, Endothelial cells, Langerhans cells, Macrophages
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
CD Molecules
Antigen References

1. Wileman TE, et al. 1986. P. Natl. Acad. Sci. USA 83:2501.
2. Apostolopoulos V, et al. 2001. Curr. Mol. Med. 1:469.
3. Burgdorf S, et al. 2006. J. Immunol. 176:6770.
4. McKenzie EJ, et al. 2007. J. Immunol. 178:4975.

Gene ID
17533 View all products for this Gene ID
UniProt
View information about CD206 on UniProt.org

Related FAQs

Why is mouse CD206 stained intracellularly and not via surface staining?

Typically, mouse CD206 surface level is relatively low under normal conditions and so intracellular staining protocol is required to get better signal.

Other Formats

View All CD206 Reagents Request Custom Conjugation
Description Clone Applications
Brilliant Violet 711™ anti-mouse CD206 (MMR) C068C2 ICFC,FC
Brilliant Violet 785™ anti-mouse CD206 (MMR) C068C2 ICFC,FC
PE/Dazzle™ 594 anti-mouse CD206 (MMR) C068C2 ICFC,FC
Alexa Fluor® 700 anti-mouse CD206 (MMR) C068C2 ICFC,FC
Spark YG™ 570 anti-mouse CD206 (MMR) C068C2 IHC-F
Biotin anti-mouse CD206 (MMR) C068C2 ICFC,FC
Purified anti-mouse CD206 (MMR) C068C2 ICFC,FC
FITC anti-mouse CD206 (MMR) C068C2 ICFC,FC
PE anti-mouse CD206 (MMR) C068C2 ICFC,FC
APC anti-mouse CD206 (MMR) C068C2 ICFC,FC
Alexa Fluor® 488 anti-mouse CD206 (MMR) C068C2 ICFC,FC,IHC-F
Alexa Fluor® 647 anti-mouse CD206 (MMR) C068C2 ICFC,FC,IHC-F
PerCP/Cyanine5.5 anti-mouse CD206 (MMR) C068C2 ICFC,FC
PE/Cyanine7 anti-mouse CD206 (MMR) C068C2 ICFC,FC
Brilliant Violet 421™ anti-mouse CD206 (MMR) C068C2 ICFC,FC,IHC-F,SB
Brilliant Violet 605™ anti-mouse CD206 (MMR) C068C2 ICFC,FC
Brilliant Violet 650™ anti-mouse CD206 (MMR) C068C2 ICFC,FC
Alexa Fluor® 594 anti-mouse CD206 (MMR) C068C2 IHC-F
PE/Cyanine5 anti-mouse CD206 (MMR) C068C2 ICFC,FC
PE/Fire™ 700 anti-mouse CD206 (MMR) C068C2 ICFC,FC
TotalSeq™-B0173 anti-mouse CD206 (MMR) C068C2 ICPG
Spark Red™ 718 anti-mouse CD206 (MMR) (Flexi-Fluor™) C068C2 FC
Spark Blue™ 574 anti-mouse CD206 (MMR) (Flexi-Fluor™) C068C2 FC
TotalSeq™-C0173 anti-mouse CD206 (MMR) Antibody C068C2 ICPG
Spark YG™ 581 anti-mouse CD206 (MMR) (Flexi-Fluor™) C068C2 FC
Spark YG™ 593 anti-mouse CD206 (MMR) (Flexi-Fluor™) Antibody C068C2 FC
Spark NIR™ 685 anti-mouse CD206 (MMR) (Flexi-Fluor™) Antibody C068C2 FC
Go To Top Version: 3    Revision Date: 04/04/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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