APC anti-mouse TCR β chain Antibody APC anti-mouse TCR β chain Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

Affinity purified TCR from mouse DO-11.10 cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Red Laser (633 nm)

Application Notes

H57-597 is a hamster mAb directed to an epitope of the C region of TCR ß chain¹². The H57-597 antibody does not cross-react with ?/d TCR-bearing T cells. Immobilized or soluble H57-597 antibody can activate a/ß TCR-bearing T cells. Additional reported applications (for the relevant formats) for this antibody include: immunoprecipitation², in vitro stimulation^2,3, in vivo depletion^4-6, and immunohistochemical staining of acetone-fixed frozen sections^7,8,9. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 109253-109258).

Application References

1. Gascoigne NJ. 1990. J. Biol. Chem. 265:9296.
2. Kruisbeek A, et al. 1991. In Current Protocols in Immunology. pp. 3.12.1. (Costim IP)
3. Davenport C, et al. 1995. J. Immunol. 155:3742. (Costim)
4. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
5. Kummer U, et al. 2001. Immunol. Lett. 75:153. (Deplete)
6. van der Heyde HC, et al. 1995. J. Immunol. 154:3985. (Deplete)
7. Tomita K, et al. 1999. Genes Dev. 13:1203. (IHC)
8. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
9. Ponomarev ED, et al. 2007. J. Immunol. 178:39. (IHC)
10. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
11. Tsukumo S, et al. 2006. J.Immunol. 177:8365. (FC) PubMed
12. Grégoire C, et al. 1991. Proc. Natl. Acad. Sci USA 88:8077.

Product Citations

1. Kisielow J, et al. 2019. Nat Immunol. 1.286111111. PubMed
2. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
3. Collin R, et al. 2020. J Immunol. 205:133. PubMed
4. Omer-Javed A, et al. 2022. Cell. 185:2248. PubMed
5. Horkova V, et al. 2023. Nat Immunol. 24:174. PubMed
6. Jin SM, et al. 2023. Nat Nanotechnol. :. PubMed
7. Zindl CL, et al. 2022. Immunity. 55:494. PubMed
8. Tate T, et al. 2023. Cancers (Basel). 15:. PubMed
9. Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed
10. Ferrari de Andrade L, et al. 2020. Cancer Immunol Res. 0.867361111. PubMed
11. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
12. Shibata K, et al. 2022. Nat Commun. 13:6948. PubMed
13. Nesterenko PA, et al. 2021. Cell Rep. 37:110167. PubMed
14. Lee H, et al. 2020. Cell Metabolism. 31(4):822-836. PubMed
15. Baranek T, et al. 2020. Cell Reports. 32(10):108116. PubMed
16. Andrews D, et al. 2008. J Virol. 82:4931. PubMed
17. Ma KY, et al. 2021. Nat Immunol. 22:1590. PubMed
18. Isoda T et al. 2017. Cell. 171(1):103-119 . PubMed
19. Schwartz DM et al. 2019. Immunity. 50(1):106-120 . PubMed
20. Chen L, et al. 2018. Immunology. 155:123. PubMed
21. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
22. Porsche CE, et al. 2021. JCI Insight. 6:. PubMed
23. Runge EM, et al. 2020. J Neuroinflammation. 17:121. PubMed
24. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
25. Bunn P, et al. 2014. J Immunol. 192:3709. PubMed
26. Mahadevan NR, et al. 2021. Cancer Discov. 11:1952. PubMed
27. Yue X, et al. 2019. Nat Commun. 10:2011. PubMed
28. Hanasoge Somasundara AV, et al. 2021. Cell Rep. 37:110099. PubMed
29. Knizkova D, et al. 2022. Nat Immunol. 23:1644. PubMed
30. Horkova V, et al. 2020. Cell Reports. 30(5):1504-1514.e7.. PubMed
31. Mallis R, et al. 2015. Proc Natl Acad Sci U S A. 112: 8373 - 8378. PubMed
32. Kondo M, et al. 2016. J Immunol. 196: 563 - 572. PubMed
33. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
34. Georgoudaki A, et al. 2016. Cell Rep. 15: 2000-2011. PubMed
35. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
36. Muri J, et al. 2020. eLife. 9:e53627.. PubMed
37. Chuang YT, et al. 2019. J Allergy Clin Immunol. 143:565. PubMed
38. Metidji A et al. 2018. Immunity. 49(2):353-362 . PubMed
39. Amezcua Vesely MC, et al. 2020. Cell. 178(5):1176-1188.e15.. PubMed
40. Bajaña S, et al. 2022. iScience. 25:103732. PubMed
41. Müller TR, et al. 2021. Cell Rep Med. 2:100374. PubMed
42. Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed
43. Sparber F, et al. 2019. Cell Host Microbe. 25:389. PubMed
44. Tsyklauri O, et al. 2021. EMBO Rep. 22:e50785. PubMed
45. Zhu M, et al. 2012. J Biol Chem. 287:8135. PubMed
46. Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed
47. Kanuma T, et al. 2017. The Journal of Immunology. 10.4049/jimmunol.1600731. PubMed
48. Baptista M, et al. 2016. Nat Commun. 7:12175. PubMed
49. Preece R, et al. 2020. Mol Ther Methods Clin Dev. 0.895138889. PubMed
50. Andrews D, et al. 2010. J Exp Med. 207:1333. PubMed
51. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
52. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
53. Zhang B, et al. 2021. Nature. 599:471. PubMed
54. Meng Q, et al. 2021. J Immunother Cancer. 9:. PubMed
55. Hsu HP, et al. 2021. J Biol Chem. 296:100419. PubMed
56. Szeto C, et al. 2022. Nat Commun. 13:4951. PubMed
57. Ley S 2009. J Immunol. 183:865. PubMed
58. Omenetti S, et al. 2019. Immunity. 51:77. PubMed
59. Kisielow J, et al. 2019. Nat Immunol. 1.286111111. PubMed
60. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
61. Collin R, et al. 2020. J Immunol. 205:133. PubMed
62. Omer-Javed A, et al. 2022. Cell. 185:2248. PubMed
63. Horkova V, et al. 2023. Nat Immunol. 24:174. PubMed
64. Jin SM, et al. 2023. Nat Nanotechnol. :. PubMed
65. Zindl CL, et al. 2022. Immunity. 55:494. PubMed
66. Tate T, et al. 2023. Cancers (Basel). 15:. PubMed
67. Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed
68. Ferrari de Andrade L, et al. 2020. Cancer Immunol Res. 0.867361111. PubMed
69. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
70. Shibata K, et al. 2022. Nat Commun. 13:6948. PubMed
71. Nesterenko PA, et al. 2021. Cell Rep. 37:110167. PubMed
72. Lee H, et al. 2020. Cell Metabolism. 31(4):822-836. PubMed
73. Baranek T, et al. 2020. Cell Reports. 32(10):108116. PubMed
74. Andrews D, et al. 2008. J Virol. 82:4931. PubMed
75. Ma KY, et al. 2021. Nat Immunol. 22:1590. PubMed
76. Isoda T et al. 2017. Cell. 171(1):103-119 . PubMed
77. Schwartz DM et al. 2019. Immunity. 50(1):106-120 . PubMed
78. Chen L, et al. 2018. Immunology. 155:123. PubMed
79. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
80. Porsche CE, et al. 2021. JCI Insight. 6:. PubMed
81. Runge EM, et al. 2020. J Neuroinflammation. 17:121. PubMed
82. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
83. Bunn P, et al. 2014. J Immunol. 192:3709. PubMed
84. Mahadevan NR, et al. 2021. Cancer Discov. 11:1952. PubMed
85. Yue X, et al. 2019. Nat Commun. 10:2011. PubMed
86. Hanasoge Somasundara AV, et al. 2021. Cell Rep. 37:110099. PubMed
87. Knizkova D, et al. 2022. Nat Immunol. 23:1644. PubMed
88. Horkova V, et al. 2020. Cell Reports. 30(5):1504-1514.e7.. PubMed
89. Mallis R, et al. 2015. Proc Natl Acad Sci U S A. 112: 8373 - 8378. PubMed
90. Kondo M, et al. 2016. J Immunol. 196: 563 - 572. PubMed
91. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
92. Georgoudaki A, et al. 2016. Cell Rep. 15: 2000-2011. PubMed
93. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
94. Muri J, et al. 2020. eLife. 9:e53627.. PubMed
95. Chuang YT, et al. 2019. J Allergy Clin Immunol. 143:565. PubMed
96. Metidji A et al. 2018. Immunity. 49(2):353-362 . PubMed
97. Amezcua Vesely MC, et al. 2020. Cell. 178(5):1176-1188.e15.. PubMed
98. Bajaña S, et al. 2022. iScience. 25:103732. PubMed
99. Müller TR, et al. 2021. Cell Rep Med. 2:100374. PubMed
100. Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed
101. Sparber F, et al. 2019. Cell Host Microbe. 25:389. PubMed
102. Tsyklauri O, et al. 2021. EMBO Rep. 22:e50785. PubMed
103. Zhu M, et al. 2012. J Biol Chem. 287:8135. PubMed
104. Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed
105. Kanuma T, et al. 2017. The Journal of Immunology. 10.4049/jimmunol.1600731. PubMed
106. Baptista M, et al. 2016. Nat Commun. 7:12175. PubMed
107. Preece R, et al. 2020. Mol Ther Methods Clin Dev. 0.895138889. PubMed
108. Andrews D, et al. 2010. J Exp Med. 207:1333. PubMed
109. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
110. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
111. Zhang B, et al. 2021. Nature. 599:471. PubMed
112. Meng Q, et al. 2021. J Immunother Cancer. 9:. PubMed
113. Hsu HP, et al. 2021. J Biol Chem. 296:100419. PubMed
114. Szeto C, et al. 2022. Nat Commun. 13:4951. PubMed
115. Ley S 2009. J Immunol. 183:865. PubMed
116. Omenetti S, et al. 2019. Immunity. 51:77. PubMed

RRID

AB_313434 (BioLegend Cat. No. 109211)
AB_313435 (BioLegend Cat. No. 109212)

Antigen Details

Structure

Ig superfamily, CD3/TCR complex with CD3 and TCR α subunit

Distribution

Majority of T cells and thymocytes (correlated to differentiation)

Function

Antigen recognition, T cell activation

Ligand/Receptor

Peptide bound-MHC class I and II

Antigen References

1. Davis MM, et al. 1998. Ann. Rev. Immunol. 16:523.
2. Huppa JB, et al. 2003. Nat. Immunol. 4:749.
3. Kubo R, et al. 1989. J. Immunol. 142:2736.

Gene ID

21577 View all products for this Gene ID

UniProt

View information about TCR beta chain on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Material Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

Other Formats

Certificate of Analysis