Description
CD8a is a 32 kD glycoprotein also known as T8, Lyt2, Ly-2, and CD8α. CD8a is a member of the immunoglobulin superfamily expressed on most thymocytes, subset of mature T cells, most NK cells, macrophages, and some activated CD4+ T cells (not resting). CD8a forms heterodimers with the CD8β chain (CD8b) on the surface of most thymocytes, while mature peripheral T lymphocytes express almost exclusively the CD8 αβ heterodimer. Intestinal intraepithelial lymphocytes express CD8a without CD8b. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 participates in T cell activation through its association with the T cell receptor complex and protein tyrosine kinase lck (p56lck).
Product Details
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser
Red Laser (633 nm)
Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1, blocking1 of allogeneic responses, and immunohistochemical staining of acetone-fixed frozen sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 200612).
Application References
Torres-Nagel N, et al. 1992. Eur. J. Immunol. 22:2841.
Matta B, et al. 2008. Am J Pathol. 173:1440. PubMed
Product Citations
Zhang J, et al. 2016. Mutat Res Genet Toxicol Environ Mutagen. 795:36-40. PubMed
Most thymocytes, T cell subset; activated CD4+ T cells (not resting), most NK cells and intestinal intraepithelial cells express the CD8α chain without the CD8β chain
Function
Co-receptor for TCR
Ligand/Receptor
MHC class I molecule
Cell Type
Thymocytes, T cells, NK cells, Epithelial cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References
1. Brideau RJ, et al. 1980. Eur. J. Immunol. 10:609. 2. Dallman MJ, et al. 1982. Eur. J. Immunol. 12:511. 3. Cantrell DA, et al. 1982. Immunol. 45:97. 4. Lyscom N, et al. 1982. Immunol. 45:775. 5. Johnson P, et al. 1985. EMBO. J. 4:2539. 6. Barclay AN. 1981. Immunol. 42:593.
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
Description
CD8a is a 32 kD glycoprotein also known as T8, Lyt2, Ly-2, and CD8α. CD8a is a member of the immunoglobulin superfamily expressed on most thymocytes, subset of mature T cells, most NK cells, macrophages, and some activated CD4+ T cells (not resting). CD8a forms heterodimers with the CD8β chain (CD8b) on the surface of most thymocytes, while mature peripheral T lymphocytes express almost exclusively the CD8 αβ heterodimer. Intestinal intraepithelial lymphocytes express CD8a without CD8b. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 participates in T cell activation through its association with the T cell receptor complex and protein tyrosine kinase lck (p56lck).
Product Details
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser
Red Laser (633 nm)
Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1, blocking1 of allogeneic responses, and immunohistochemical staining of acetone-fixed frozen sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 200612).
Application References
Torres-Nagel N, et al. 1992. Eur. J. Immunol. 22:2841.
Matta B, et al. 2008. Am J Pathol. 173:1440. PubMed
Product Citations
Zhang J, et al. 2016. Mutat Res Genet Toxicol Environ Mutagen. 795:36-40. PubMed
Most thymocytes, T cell subset; activated CD4+ T cells (not resting), most NK cells and intestinal intraepithelial cells express the CD8α chain without the CD8β chain
Function
Co-receptor for TCR
Ligand/Receptor
MHC class I molecule
Cell Type
Thymocytes, T cells, NK cells, Epithelial cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References
1. Brideau RJ, et al. 1980. Eur. J. Immunol. 10:609. 2. Dallman MJ, et al. 1982. Eur. J. Immunol. 12:511. 3. Cantrell DA, et al. 1982. Immunol. 45:97. 4. Lyscom N, et al. 1982. Immunol. 45:775. 5. Johnson P, et al. 1985. EMBO. J. 4:2539. 6. Barclay AN. 1981. Immunol. 42:593.
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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