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Brilliant Violet 421™ Streptavidin Brilliant Violet 421™ Streptavidin

Pricing & Availability
Regulatory Status
RUO
Other Names
SAv-Brilliant Violet 421™
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Product Citations
publications
SAV_BV421_071911
Human peripheral blood lymphocytes were stained with biotinylated CD3 (filled histogram) or mouse IgG1 isotype control (open histogram), followed with SAV-Brilliant Violet 421™.
  • SAV_BV421_071911
    Human peripheral blood lymphocytes were stained with biotinylated CD3 (filled histogram) or mouse IgG1 isotype control (open histogram), followed with SAV-Brilliant Violet 421™.
See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
405226 100 µL DKK1147
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405225 100 µg DKK2745
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Description

Streptavidin binds to biotin with high affinity. Streptavidin-Brilliant Violet 421™ is useful for detecting biotinylated antibodies. The excitation of Brilliant Violet 421™ by 405 nm laser light induces a fluorescence maximum emission of 421 nm.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Cat. No. 405225: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Cat. No. 405226: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
Streptavidin was conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
405225 is bottled at 0.5 mg/ml.
405226 is bottled at 0.1 mg/ml.
(concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC, ICFC - Quality tested
ICC, IHC-F, IHC-P
Recommended Usage

Each lot of this Streptavidin-Brilliant Violet 421™ is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Brilliant Violet 421™ conjugation. For immunofluorescent staining, we recommend using Cat. No. 405226 at dilutions ≤ 0.015 µg in 100 µL staining volume per million cells. For applications requiring high concentration of streptavidin reagent such as tetramer labeling, we recommend using Cat. No. 405225. It is recommended that the reagent be titrated for optimal performance for each application.

* Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Streptavidin-Brilliant Violet 421™ is useful as a second step reagent for indirect immunofluorescent staining when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-Brilliant Violet 421™ is 340 kD and Streptavidin alone is 52 kD.

Additional Product Notes

View more applications data using this product in immunofluorescence microscopy and labeling tetramers in flow cytometry in our Scientific Poster Library.

Application References
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  165. Ogishi M, et al. 2023. J Exp Med. 220: . PubMed
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  171. Meyer S, et al. 2023. Cell Rep. 42:111995. PubMed
  172. Zhang S, et al. 2023. J Clin Med. 12: . PubMed
  173. Yeh CH, et al. 2022. Immunity. 55:272. PubMed
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  183. Lu T, et al. 2023. J Med Virol. 95:e28730. PubMed
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  189. Miura M, et al. 2019. Wellcome Open Res. 3:105. PubMed
  190. Hayashi M, et al. 2017. EBioMedicine. 15:127-136. PubMed
  191. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
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  194. Kato T, et al. 2019. Front Immunol. 10:2021. PubMed
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  196. Xing Y, et al. 2014. J Neurosci. 34:14128. PubMed
  197. Sei J, et al. 2016. PLoS One. 11: 0152192. PubMed
  198. Li H, et al. 2016. J Immunol. 196: 4064 - 4074. PubMed
  199. Sage P, et al. 2016. Nat Immunol. 17:1436-1446. PubMed
  200. Xiong W, et al. 2021. Int J Mol Sci. 22:. PubMed
  201. Hu W, et al. 2021. Nat Immunol. 22:1163. PubMed
  202. Krishack PA, et al. 2019. JCI Insight. 4:6. PubMed
  203. Mitra T, et al. 2017. Vaccine. 10.1016/j.vaccine.2017.06.035. PubMed
  204. Trivedi S, et al. 2015. Vaccine. 33: 7315-7327. PubMed
  205. Sugiyama D, et al. 2013. Proc Natl Acad Sci U S A. 110:17945. PubMed
  206. Giltiay N, et al. 2013. J Immunol. 191:2155. PubMed
  207. Poon MML, et al. 2021. Sci Immunol. 6:eabl9105. PubMed
  208. Petursdottir D, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01699. PubMed
  209. Zhao M, et al. 2019. Cell Rep. 26:652. PubMed
  210. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  211. Wu J, et al. 2020. Sci Adv. 6:eaba3458. PubMed
  212. Li T, et al. 2022. Int J Infect Dis. 122:874. PubMed
  213. Fachi JL, et al. 2020. J Exp Med. 217:00:00. PubMed
  214. Im S, et al. 2016. Nature. 537:417-421. PubMed
  215. Poh C, et al. 2014. Infect Immun . 82:4854. PubMed
  216. Nakagawa T, et al. 2021. Cell Rep. 37:109875. PubMed
  217. Gaudette BT, et al. 2021. J Clin Invest. 131:. PubMed
  218. Cai D, et al. 2021. Front Mol Biosci. 8:686803. PubMed
  219. Neumann B, et al. 2019. Cell Stem Cell. 25:473. PubMed
  220. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  221. Wilmore JR et al. 2018. Cell host & microbe. 23(3):302-311 . PubMed
  222. Koay H, et al. 2016. Nat Immunol. 10.1038/ni.3565. PubMed
  223. Lucas A, et al. 2015. PLoS One. 10:117160. PubMed
  224. Zhang R, et al. 2021. MAbs. 13:1904546. PubMed
  225. Taguchi A, et al. 2017. Cell Stem Cell. 21:730. PubMed
  226. Hansen UK, et al. 2020. Front Immunol. 11:373. PubMed
  227. Krishnaswamy J, et al. 2015. Proc Natl Acad Sci U S A. 112:3056. PubMed
  228. Cameron G, et al. 2015. J Immunol. 195: 4604 - 4614. PubMed
  229. Rowan A, et al. 2016. PLoS Pathog. 12:e1006030. PubMed
  230. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  231. Bähr A, et al. 2016. PLoS One. 11: 0155676. PubMed
  232. Xu Z, et al. 2016. Nat Commun. 7:10728. PubMed
  233. Liu B, et al. 2015. J Immunol. 194:3583. PubMed
  234. Lin C, et al. 2022. Nat Commun. 13:6869. PubMed
  235. Tarke A, et al. 2022. Cell. 185:847. PubMed
  236. Nosho S, et al. 2020. Int J Mol Sci. 21:00. PubMed
  237. Alameh MG, et al. 2021. Immunity. 54:2877. PubMed
  238. Cavazzoni CB, et al. 2022. Cell Rep. 38:110399. PubMed
  239. Delacher M, et al. 2020. Immunity. 52:295. PubMed
  240. Roberts E, et al. 2016. PLoS One. 11:e0168488. PubMed
  241. Elayeb R, et al. 2016. Haematologica. 101: 209 - 218. PubMed
  242. Cuenca M, et al. 2016. J Immunol. 196: 726 - 737. PubMed
  243. Weijer M, et al. 2014. Nat Commun. 5:3832. PubMed
  244. Xi‐Zhi J Guo et al. 2018. Immunity. 49(3):531-544 . PubMed
  245. Parra-Sánchez H, et al. 2018. Mol Immunol. 96:1. PubMed
  246. Sanjo H, et al. 2015. PLoS One. 10: 0128761. PubMed
  247. Ma X, et al. 2020. Immunity. 53:1315. PubMed
  248. Hasegawa T, et al. 2020. Sci Rep. 10:13480. PubMed
  249. Borroto A, et al. 2022. Front Immunol. 13:799919. PubMed
  250. Song R, et al. 2022. Front Immunol. 13:1028246. PubMed
  251. Blomberg R, et al. 2019. Matrix Biol. 83:60. PubMed
  252. Yang E, et al. 2016. J Immunol. 197: 934 - 941. PubMed
  253. Salzberger W, et al. 2015. PLoS One. 10: 0145324. PubMed
  254. Tsuzuki S, et al. 2015. Int Immunol. 10.1093/intimm/dxv058. PubMed
  255. Cabrera-Perez C, et al. 2015. J Immunol . 194:1609-20. PubMed
  256. Scally S, et al. 2013. J Exp Med. 210:2569. PubMed
  257. Cirelli KM et al. 2019. Cell. 177(5):1153-1171 . PubMed
  258. Wen J, et al. 2020. Cell Rep. 31:107566. PubMed
  259. Claudepierre M, et al. 2014. J Virol. 88:5242. PubMed
  260. Friedman R, et al. 2014. Proc Natl Acad Sci U S A. 111:9223. PubMed
  261. Lundell A, et al. 2017. Sci Rep. 7:39904. PubMed
  262. Fra-Bido S, et al. 2021. STAR Protocols. 2(3):100499. PubMed
  263. Wang Z, et al. 2022. J Exp Med. 219:. PubMed
  264. Guelen L, et al. 2022. J Immunother Cancer. 10:. PubMed
  265. Dan JM, et al. 2021. Science. :371. PubMed
  266. Mise-Omata S, et al. 2014. Int Immunol. 26:607. PubMed
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  268. Lederer K, et al. 2022. Cell. . PubMed
  269. Müller TR, et al. 2021. Cell Rep Med. 2:100374. PubMed
  270. An X, et al. 2017. PLoS One.. 10.1371/journal.pone.0181904. PubMed
  271. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  272. Luetke-Eversloh M, et al. 2014. PLoS Pathog. 10:1004441. PubMed
  273. Lang P 2015. Int Immunol. 27:253. PubMed
  274. Younis R, et al. 2016. J Immunol. 196: 1419 - 1429. PubMed
  275. Mchedlidze T, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.20. PubMed
  276. Zhu L, et al. 2016. J Cell Sci. 129: 4238 - 4251. PubMed
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  278. Clement RL, et al. 2019. Nat Immunol. 20:1360. PubMed
  279. Kim JM, et al. 2020. Nat Commun. 11:2289. PubMed
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  286. Mahmoudi S, et al. 2019. Nature. 574:553. PubMed
  287. Lyngaa R, et al. 2014. Clin Cancer Res. 20:1768. PubMed
  288. Choo J, et al. 2014. J Virol. 88:10613. PubMed
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  291. Binnewies M, et al. 2019. Cell. 177:556. PubMed
  292. Higdon L, et al. 2014. Proc Natl Acad Sci U S A. 111:5652. PubMed
  293. Kim G, et al. 2013. J Immunol. 190:1510. PubMed
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Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 8    Revision Date: 12/18/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Pricing & Availability
Regulatory Status
RUO
Other Names
SAv-Brilliant Violet 421™
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Product Citations
publications
SAV_BV421_071911
Human peripheral blood lymphocytes were stained with biotinylated CD3 (filled histogram) or mouse IgG1 isotype control (open histogram), followed with SAV-Brilliant Violet 421™.
  • SAV_BV421_071911
    Human peripheral blood lymphocytes were stained with biotinylated CD3 (filled histogram) or mouse IgG1 isotype control (open histogram), followed with SAV-Brilliant Violet 421™.
See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
405226 100 µL DKK1147
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405225 100 µg DKK2745
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Description

Streptavidin binds to biotin with high affinity. Streptavidin-Brilliant Violet 421™ is useful for detecting biotinylated antibodies. The excitation of Brilliant Violet 421™ by 405 nm laser light induces a fluorescence maximum emission of 421 nm.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Cat. No. 405225: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Cat. No. 405226: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
Streptavidin was conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
405225 is bottled at 0.5 mg/ml.
405226 is bottled at 0.1 mg/ml.
(concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC, ICFC - Quality tested
ICC, IHC-F, IHC-P
Recommended Usage

Each lot of this Streptavidin-Brilliant Violet 421™ is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Brilliant Violet 421™ conjugation. For immunofluorescent staining, we recommend using Cat. No. 405226 at dilutions ≤ 0.015 µg in 100 µL staining volume per million cells. For applications requiring high concentration of streptavidin reagent such as tetramer labeling, we recommend using Cat. No. 405225. It is recommended that the reagent be titrated for optimal performance for each application.

* Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Streptavidin-Brilliant Violet 421™ is useful as a second step reagent for indirect immunofluorescent staining when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-Brilliant Violet 421™ is 340 kD and Streptavidin alone is 52 kD.

Additional Product Notes

View more applications data using this product in immunofluorescence microscopy and labeling tetramers in flow cytometry in our Scientific Poster Library.

Application References
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  127. Mchedlidze T, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.20. PubMed
  128. Zhu L, et al. 2016. J Cell Sci. 129: 4238 - 4251. PubMed
  129. Fries CN, et al. 2021. Sci Rep. 11:14494. PubMed
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  135. Chryplewicz A, et al. 2022. Cancer Cell. 40:1111. PubMed
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  138. Mahmoudi S, et al. 2019. Nature. 574:553. PubMed
  139. Lyngaa R, et al. 2014. Clin Cancer Res. 20:1768. PubMed
  140. Choo J, et al. 2014. J Virol. 88:10613. PubMed
  141. Xiao C, et al. 2021. STAR Protoc. 2:100789. PubMed
  142. Rodrigues KA, et al. 2021. Sci Adv. 7:eabj6538. PubMed
  143. Binnewies M, et al. 2019. Cell. 177:556. PubMed
  144. Higdon L, et al. 2014. Proc Natl Acad Sci U S A. 111:5652. PubMed
  145. Kim G, et al. 2013. J Immunol. 190:1510. PubMed
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  147. Brunk F, et al. 2021. Eur J Immunol. 51:2651. PubMed
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  150. van Eeden C, et al. 2018. Cell Rep. 25:3123. PubMed
  151. Rad S M AH, et al. 2020. PLoS One. 15:e0232915. PubMed
  152. Takizawa F, et al. 2016. J Immunol. 196: 4522 - 4535. PubMed
  153. Steines L, et al. 2021. Front Immunol. 12:657894. PubMed
  154. García-Alegria E, et al. 2021. STAR Protoc. 2:100367. PubMed
  155. Reyes RA, et al. 2021. PLoS One. 16:e0261656. PubMed
  156. Combes AJ, et al. 2022. Cell. 185:184. PubMed
  157. He T, et al. 2022. Liver Int. 42:1287. PubMed
  158. Lee JH, et al. 2022. Cell Rep. 38:110485. PubMed
  159. Wolf EW, et al. 2022. Proc Natl Acad Sci U S A. 119:e2201794119. PubMed
  160. Tátrai P, et al. 2022. EMBO J. 41:e108739. PubMed
  161. Lei X, et al. 2022. J Exp Med. 219: . PubMed
  162. Gupta S, et al. 2022. Sci Adv. 8:eabn4886. PubMed
  163. Wang Z, et al. 2022. J Exp Med. 219: . PubMed
  164. Buckner CM, et al. 2022. Cell. 185:4333. PubMed
  165. Ogishi M, et al. 2023. J Exp Med. 220: . PubMed
  166. Scharf L, et al. 2023. JCI Insight. 8: . PubMed
  167. Touizer E, et al. 2023. iScience. 26:105862. PubMed
  168. Weskamm LM, et al. 2022. STAR Protoc. 3:101902. PubMed
  169. Bettini E, et al. 2022. STAR Protoc. 3:101840. PubMed
  170. Guo Q, et al. 2023. Front Public Health. 10:1067342. PubMed
  171. Meyer S, et al. 2023. Cell Rep. 42:111995. PubMed
  172. Zhang S, et al. 2023. J Clin Med. 12: . PubMed
  173. Yeh CH, et al. 2022. Immunity. 55:272. PubMed
  174. Tas JMJ, et al. 2022. Immunity. 55:1856. PubMed
  175. Sun P, et al. 2022. iScience. 25:105202. PubMed
  176. Tan GMY, et al. 2022. Cancers (Basel). 14:. PubMed
  177. Vyasamneni R, et al. 2023. Cell Rep Methods. 3:100388. PubMed
  178. Kawai H, et al. 2023. JBMR Plus. 7:e10722. PubMed
  179. Nihei Y, et al. 2023. Sci Adv. 9:eadd6734. PubMed
  180. Marcial-Juárez E, et al. 2023. iScience. 26:106310. PubMed
  181. Liu KY, et al. 2023. Environ Health Perspect. 131:37014. PubMed
  182. Ramalingam P, et al. 2023. Nat Commun. 14:2018. PubMed
  183. Lu T, et al. 2023. J Med Virol. 95:e28730. PubMed
  184. Fadzeyeva E, et al. 2023. iScience. 26:106748. PubMed
  185. Sigmund EC, et al. 2023. PLoS One. 18:e0285597. PubMed
  186. Zhang AQ, et al. 2023. Nat Biomed Eng. . PubMed
  187. Domingues AF, et al. 2020. Elife. 9:e51754. PubMed
  188. Parra–Sánchez H, et al. 2019. Viruses. 1.153472222. PubMed
  189. Miura M, et al. 2019. Wellcome Open Res. 3:105. PubMed
  190. Hayashi M, et al. 2017. EBioMedicine. 15:127-136. PubMed
  191. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
  192. Li H, et al. 2022. Front Immunol. 13:988004. PubMed
  193. Chaugule S, et al. 2021. Int J Mol Sci. 22:. PubMed
  194. Kato T, et al. 2019. Front Immunol. 10:2021. PubMed
  195. Viborg N, et al. 2019. Oncoimmunology. 8:e1663107. PubMed
  196. Xing Y, et al. 2014. J Neurosci. 34:14128. PubMed
  197. Sei J, et al. 2016. PLoS One. 11: 0152192. PubMed
  198. Li H, et al. 2016. J Immunol. 196: 4064 - 4074. PubMed
  199. Sage P, et al. 2016. Nat Immunol. 17:1436-1446. PubMed
  200. Xiong W, et al. 2021. Int J Mol Sci. 22:. PubMed
  201. Hu W, et al. 2021. Nat Immunol. 22:1163. PubMed
  202. Krishack PA, et al. 2019. JCI Insight. 4:6. PubMed
  203. Mitra T, et al. 2017. Vaccine. 10.1016/j.vaccine.2017.06.035. PubMed
  204. Trivedi S, et al. 2015. Vaccine. 33: 7315-7327. PubMed
  205. Sugiyama D, et al. 2013. Proc Natl Acad Sci U S A. 110:17945. PubMed
  206. Giltiay N, et al. 2013. J Immunol. 191:2155. PubMed
  207. Poon MML, et al. 2021. Sci Immunol. 6:eabl9105. PubMed
  208. Petursdottir D, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01699. PubMed
  209. Zhao M, et al. 2019. Cell Rep. 26:652. PubMed
  210. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  211. Wu J, et al. 2020. Sci Adv. 6:eaba3458. PubMed
  212. Li T, et al. 2022. Int J Infect Dis. 122:874. PubMed
  213. Fachi JL, et al. 2020. J Exp Med. 217:00:00. PubMed
  214. Im S, et al. 2016. Nature. 537:417-421. PubMed
  215. Poh C, et al. 2014. Infect Immun . 82:4854. PubMed
  216. Nakagawa T, et al. 2021. Cell Rep. 37:109875. PubMed
  217. Gaudette BT, et al. 2021. J Clin Invest. 131:. PubMed
  218. Cai D, et al. 2021. Front Mol Biosci. 8:686803. PubMed
  219. Neumann B, et al. 2019. Cell Stem Cell. 25:473. PubMed
  220. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
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Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 8    Revision Date: 12/18/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

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