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CFSE Cell Division Tracker Kit CFSE Cell Division Tracker Kit

Pricing & Availability
Regulatory Status
RUO
Other Names
5-(and 6)-Carboxyfluorescein diacetate succinimidyl ester Kit, CFDA SE Kit
Ave. Rating
Submit a Review
Product Citations
publications
CFSE_Cell_Tracking_Kit_022014
Human peripheral blood mononuclear cells were stained with CFSE Cell Division Tracking Kit, and then stimulated with (filled histogram) or without (open histogram) PHA for 5 days. On day 5, cells were harvested and the CFSE fluorescent staining was analyzed by flow cytometry.
  • CFSE_Cell_Tracking_Kit_022014
    Human peripheral blood mononuclear cells were stained with CFSE Cell Division Tracking Kit, and then stimulated with (filled histogram) or without (open histogram) PHA for 5 days. On day 5, cells were harvested and the CFSE fluorescent staining was analyzed by flow cytometry.
Cat # Size Price Quantity Check Availability Save
423801 1 kit DKK600
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CFSE Cell Division Tracker Kit is composed of 5 vials, 100 µg per vial of CFSE (formally known as 5-(and 6)-Carboxyfluorescein diacetate succinimidyl ester of CFDA SE), and 500 µl of anhydrous DMSO. CFSE is able to passively diffuse into cells. Inside the cell, its acetate groups are cleaved by intracellular esterases, and the molecules are converted to fluorescent esters. CFSE is retained within the cell and covalently couples to intracellular molecules via its succinimidyl group. Due to this covalent coupling reaction, fluorescent CFSE can be retained within the cell for an extremely long period. Also, due to this stable linkage, once the dye has been incorporated within the cell, it is not transferred to adjacent cells. CFSE is widely used for cell proliferation assays and in vivo cell tracking.

Product Details
Technical Data Sheet (pdf)

Product Details

Preparation
The CFSE Cell Division Tracker Kit is composed of lyophilized CFSE and anhydrous DMSO. For reconstitution, bring the kit to room temperature; add 36 µl of DMSO to one vial of CFSE dye until fully dissolved.
Storage & Handling
Store CFSE Cell Division Tracker Kit at -20°C upon receipt. Do not open vials until needed. Once the DMSO is added to the CFSE, use immediately, or store at -20°C in a dry place and protected from light, preferably in a dessicator or in a container with desiccant for no more than one month.
Application

ICFC - Quality tested
In vivo cell tracking1 - Reported in the literature, not verified in house
Recommended Usage

This lot has been tested by flow cytometric analysis of in vitro cell proliferation assay. It can be used at concentrations ranging from 0.5 - 10 µM for cell labeling. It is recommended that the reagent be titrated for optimal performance for each cell type, culturing condition, or application.

Application Notes

The molecular weight of CFSE is 557.47. The excitation and emission wavelengths of CFSE-labeled cells are 492 nm and 517 nm, respectively. Each 100 µg vial of CFSE may be reconstituted with 36 µl of anhydrous DMSO to yield a stock concentration of 5 mM.

Materials Provided:
5 vials x 100 µg CFSE
500 µl anhydrous DMSO

CFSE Labeling Procedure: 
1. Prior to reconstitution, spin down the vial of lyophilized reagent in a microcentrofuge to ensure the reagent is at the bottom of the vial.
2. Prepare stock solution by reconstituting 1 vial of lyophilized CFSE dye in 36 µL of DMSO to make a 5 mM solution.
3. Prepare a 5 µM working solution by diluting 1 µL of 5 mM CFSE stock solution in 1 mL PBS for every 1 mL of cell suspension (or at an optimal working concentration as determined by titration).
4. Spin down and resuspend cells at 10-100 x 106 cells/mL in the CFSE working solution.
5. Incubate cells for 20 minutes at room temperature or 37°C and keep protected from light.
6. Quench the staining by adding 5 times the original staining volume of cell culture medium containing 10% FBS.
7. Pellet cells and resuspend in pre-warmed cell culture medium. 
8. Incubate cells for 10 minutes.
9. After incubation, CFSE labeled cells are ready for downstream applications or analysis.

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.


Watch a Scientific Poster video of this product.

Application References
  1. Parish CR, et al. 2009. Curr. Protoc. Immunol. Unit4.9.
  2. Miller MJ, et al. 2002. Science 296:1869.
  3. Tai LH, 2013. Clin Cancer Res. 19:5104. PubMed
  4. Svajger U, et al. 2014. J Lukoc Biol. 95:33. PubMed
Product Citations
  1. Nalbandian M, et al. 2022. Life Sci Alliance. 5: . PubMed
  2. Zhang Y, et al. 2022. Nat Med. 28:1421. PubMed
  3. Hsieh T, et al. 2022. Front Immunol. 13:901030. PubMed
  4. Liang Y, et al. 2022. Theranostics. 12:7729. PubMed
  5. Tang M, et al. 2022. Biomolecules. 12: . PubMed
  6. Zhang Z, et al. 2023. J Transl Med. 21:23. PubMed
  7. Vondra S, et al. 2023. Cell Rep. 42:111977. PubMed
  8. Garcia-Becerra N, et al. 2023. Cancers (Basel). 15: . PubMed
  9. Lin CP, et al. 2023. EMBO J. 42:e111614. PubMed
  10. Wang R, et al. 2023. iScience. 26:105954. PubMed
  11. Ma Y, et al. 2022. Am J Physiol Heart Circ Physiol. 322:H622. PubMed
  12. Sheng YR, et al. 2022. Cell Mol Life Sci. 79:173. PubMed
  13. Li Y, et al. 2022. Transl Oncol. 21:101424. PubMed
  14. Mews EA, et al. 2022. ACS Nano. 16:12185. PubMed
  15. Shen M, et al. 2022. Adv Sci (Weinh). 9:e2203523. PubMed
  16. Li L, et al. 2022. Cancer Immunol Res. 10:1475. PubMed
  17. Nasiri F, et al. 2023. Front Immunol. 14:1063838. PubMed
  18. Do-Thi VA, et al. 2023. Cancer Res Commun. 3:80. PubMed
  19. Bayat H, et al. 2023. Mol Ther Nucleic Acids. 32:432. PubMed
  20. Wu C, et al. 2023. Cancer Metab. 11:7. PubMed
  21. Romo ML, et al. 2023. PLoS Negl Trop Dis. 17:e0011386. PubMed
  22. Wang Z, et al. 2023. Mediators Inflamm. 2023:9810733. PubMed
  23. Labadie KP, et al. 2023. Cancer Gene Ther. . PubMed
  24. Ennis S, et al. 2023. iScience. 26:106943. PubMed
  25. Tocheva AS, et al. 2020. Curr Protoc Immunol. 130:e103. PubMed
  26. Boland LK, et al. 2019. Front Immunol. 10:1080. PubMed
  27. Zhang R, et al. 2021. Cell Mol Immunol. 18:1222. PubMed
  28. Page N, et al. 2018. Immunity. 48:937. PubMed
  29. Zupancic E, et al. 2017. Journal of Controlled Release. 10.1016/j.jconrel.2017.05.014. PubMed
  30. Boyt DT, et al. 2020. J R Soc Interface. 17:20190815. PubMed
  31. Wen Y, et al. 2020. J Invest Dermatol. . PubMed
  32. Hickey A, et al. 2021. Front Microbiol. 12:653587. PubMed
  33. Yuan X, et al. 2022. J Extracell Vesicles. 11:e12235. PubMed
  34. Pires D, et al. 2021. Front Immunol. 12:742822. PubMed
  35. Sawada J, et al. 2021. Am J Pathol. 191:396. PubMed
  36. Hui CW, et al. 2022. Front Immunol. 13:919854. PubMed
  37. Nguyen DT, et al. 2022. Cells. 11:. PubMed
  38. Ma C, et al. 2021. Signal Transduct Target Ther. 6:353. PubMed
  39. Saito S, et al. 2020. Nutrients. 12:. PubMed
  40. Deng H, et al. 2021. J Asthma Allergy. 14:839. PubMed
  41. Rennier K, et al. 2020. Clin Cancer Res. 26:5019. PubMed
  42. Chen X, et al. 2021. Cell Death Differ. 28:1237. PubMed
  43. Chen M, et al. 2021. Cancers (Basel). 13:. PubMed
  44. Just S, et al. 2016. Sci Rep. 6:39796. PubMed
  45. Jones N, et al. 2021. Nat Commun. 12:1209. PubMed
  46. Coray M, et al. 2022. Int J Mol Sci. 23:. PubMed
  47. Guo HZ, et al. 2021. Sci Adv. 7:eabg4167. PubMed
  48. Potluri HK, et al. 2022. J Immunother Cancer. 10:. PubMed
  49. Onodera T, et al. 2015. Sci Rep. 5:16801. PubMed
  50. Zhao J, et al. 2021. Front Immunol. 12:658420. PubMed
  51. Moser B, et al. 2021. Mol Cancer. 20:16. PubMed
  52. Hawila E, et al. 2017. Cell Rep.. 10.1016/j.celrep.2017.10.104. PubMed
  53. Wang Y, et al. 2021. Sci Rep. 1.429861111. PubMed
  54. Zhou L, et al. 2020. Clin Cancer Res. 26:290. PubMed
  55. Sertori R, et al. 2022. Front Immunol. 13:928252. PubMed
  56. Lv M, et al. 2020. Cell Res. 30:966. PubMed
  57. Codo AC, et al. 2020. Cell Metab. 32:437. PubMed
  58. Lerrer S, et al. 2021. iScience. 24:103020. PubMed
  59. Di Buduo CA, et al. 2021. eLife. 10:00. PubMed
  60. Lu Z, et al. 2022. Nat Commun. 13:6623. PubMed
  61. Yang Q, et al. 2020. Theranostics. 6.2375. PubMed
  62. Stinson WA, et al. 2022. JCI Insight. 7:. PubMed
  63. Holokai L, et al. 2020. Cancers (Basel). 12:00. PubMed
  64. Monteran L, et al. 2022. Nat Commun. 13:5797. PubMed
  65. Stirling ER, et al. 2022. J Immunother Cancer. 10:. PubMed
  66. Krueger CC, et al. 2019. Front Immunol. 10:1831. PubMed
  67. Shifrut E et al. 2018. Cell. 175(7):1958-1971 . PubMed
  68. Wei C, et al. 2016. Cell Death Dis. 7:e2489. PubMed
  69. Luo Q, et al. 2020. Cancer Sci. 111:4000. PubMed
  70. Tian M, et al. 2021. Elife. 10:. PubMed
  71. Kanemaru H, et al. 2021. iScience. 24:103067. PubMed
  72. Rodriguez RM, et al. 2019. Cell Rep. 29:860. PubMed
  73. Ho JY, et al. 2021. Mol Ther Methods Clin Dev. 21:237. PubMed
  74. Fassler M, et al. 2021. J Neuroinflammation. 18:19. PubMed
  75. Beghelli D, et al. 2017. Oxid Med Cell Longev. 10.1155/2017/7468064. PubMed
  76. Burand AJ, et al. 2020. Front Immunol. 11:143. PubMed
  77. Clement CC, et al. 2021. Immunity. 54:721. PubMed
  78. Feng Y, et al. 2022. Life (Basel). 12:. PubMed
  79. Kwiecinski JM, et al. 2021. Cell Rep. 36:109462. PubMed
  80. Nenasheva T, et al. 2017. PLoS One. 12(6):e0178983. PubMed
  81. Mercer F, et al. 2016. PLoS Negl Trop Dis. 10: 0004913. PubMed
  82. Xie X, et al. 2021. Front Immunol. 12:625808. PubMed
  83. Wettersten HI, et al. 2019. Cancer Res. 79:5048. PubMed
  84. Brockman QR, et al. 2022. JCI Insight. :. PubMed
  85. Lee SH, et al. 2022. Nat Commun. 13:5461. PubMed
  86. Kwee BJ, et al. 2019. Sci Adv. 5:eaav6313. PubMed
  87. Pires D, et al. 2021. Front Immunol. 12:647728. PubMed
  88. Chang T, et al. 2020. Aging (Albany NY). 12:21147. PubMed
  89. Lim J, et al. 2020. Sci Adv. 6:eaba1334. PubMed
  90. Chen W, et al. 2019. Arterioscler Thromb Vasc Biol. 39:2028. PubMed
  91. Montel‐Hagen A et al. 2019. Cell stem cell. 24(3):376-389 . PubMed
  92. Zheng Z, et al. 2021. Biomed Res Int. 2021:5535578. PubMed
  93. Konda P, et al. 2022. Am J Cancer Res. 12:210. PubMed
  94. Soloff A, et al. 2017. J Appl Toxicol. 10.1002/jat.3465. PubMed
  95. Sen S, et al. 2018. J Immunol. 201:440. PubMed
  96. Sung J, et al. 2015. J Infect Dis. 212: 258 - 263. PubMed
  97. Georgouli M et al. 2019. Cell. 176(4):757-774 . PubMed
  98. Rossnagl S, et al. 2016. PLoS Biol. 14: 1002562. PubMed
  99. Lhuillier C, et al. 2021. J Clin Invest. 131:. PubMed
  100. Wang N, et al. 2020. Front Immunol. 1.765972222. PubMed
  101. Zhang Z, et al. 2021. Front Immunol. 12:699478. PubMed
  102. Kwantwi LB, et al. 2021. Bioengineered. 12:6996. PubMed
  103. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
  104. Barsheshet Y, et al. 2017. Proc Natl Acad Sci U S A. 114:6086. PubMed
  105. Ward D, et al. 2016. Haematologica. 101: 286 - 296. PubMed
  106. Cao M, et al. 2021. Int J Cancer. 149:460. PubMed
  107. Ershaid N, et al. 2019. Nat Commun. 10:4375. PubMed
  108. Bonaccorsi-Riani E, et al. 2015. PLoS One. 10: 0136106. PubMed
  109. Nalbandian M, et al. 2022. Life Sci Alliance. 5: . PubMed
  110. Zhang Y, et al. 2022. Nat Med. 28:1421. PubMed
  111. Hsieh T, et al. 2022. Front Immunol. 13:901030. PubMed
  112. Liang Y, et al. 2022. Theranostics. 12:7729. PubMed
  113. Tang M, et al. 2022. Biomolecules. 12: . PubMed
  114. Zhang Z, et al. 2023. J Transl Med. 21:23. PubMed
  115. Vondra S, et al. 2023. Cell Rep. 42:111977. PubMed
  116. Garcia-Becerra N, et al. 2023. Cancers (Basel). 15: . PubMed
  117. Lin CP, et al. 2023. EMBO J. 42:e111614. PubMed
  118. Wang R, et al. 2023. iScience. 26:105954. PubMed
  119. Ma Y, et al. 2022. Am J Physiol Heart Circ Physiol. 322:H622. PubMed
  120. Sheng YR, et al. 2022. Cell Mol Life Sci. 79:173. PubMed
  121. Li Y, et al. 2022. Transl Oncol. 21:101424. PubMed
  122. Mews EA, et al. 2022. ACS Nano. 16:12185. PubMed
  123. Shen M, et al. 2022. Adv Sci (Weinh). 9:e2203523. PubMed
  124. Li L, et al. 2022. Cancer Immunol Res. 10:1475. PubMed
  125. Nasiri F, et al. 2023. Front Immunol. 14:1063838. PubMed
  126. Do-Thi VA, et al. 2023. Cancer Res Commun. 3:80. PubMed
  127. Bayat H, et al. 2023. Mol Ther Nucleic Acids. 32:432. PubMed
  128. Wu C, et al. 2023. Cancer Metab. 11:7. PubMed
  129. Romo ML, et al. 2023. PLoS Negl Trop Dis. 17:e0011386. PubMed
  130. Wang Z, et al. 2023. Mediators Inflamm. 2023:9810733. PubMed
  131. Labadie KP, et al. 2023. Cancer Gene Ther. . PubMed
  132. Ennis S, et al. 2023. iScience. 26:106943. PubMed
  133. Tocheva AS, et al. 2020. Curr Protoc Immunol. 130:e103. PubMed
  134. Boland LK, et al. 2019. Front Immunol. 10:1080. PubMed
  135. Zhang R, et al. 2021. Cell Mol Immunol. 18:1222. PubMed
  136. Page N, et al. 2018. Immunity. 48:937. PubMed
  137. Zupancic E, et al. 2017. Journal of Controlled Release. 10.1016/j.jconrel.2017.05.014. PubMed
  138. Boyt DT, et al. 2020. J R Soc Interface. 17:20190815. PubMed
  139. Wen Y, et al. 2020. J Invest Dermatol. . PubMed
  140. Hickey A, et al. 2021. Front Microbiol. 12:653587. PubMed
  141. Yuan X, et al. 2022. J Extracell Vesicles. 11:e12235. PubMed
  142. Pires D, et al. 2021. Front Immunol. 12:742822. PubMed
  143. Sawada J, et al. 2021. Am J Pathol. 191:396. PubMed
  144. Hui CW, et al. 2022. Front Immunol. 13:919854. PubMed
  145. Nguyen DT, et al. 2022. Cells. 11:. PubMed
  146. Ma C, et al. 2021. Signal Transduct Target Ther. 6:353. PubMed
  147. Saito S, et al. 2020. Nutrients. 12:. PubMed
  148. Deng H, et al. 2021. J Asthma Allergy. 14:839. PubMed
  149. Rennier K, et al. 2020. Clin Cancer Res. 26:5019. PubMed
  150. Chen X, et al. 2021. Cell Death Differ. 28:1237. PubMed
  151. Chen M, et al. 2021. Cancers (Basel). 13:. PubMed
  152. Just S, et al. 2016. Sci Rep. 6:39796. PubMed
  153. Jones N, et al. 2021. Nat Commun. 12:1209. PubMed
  154. Coray M, et al. 2022. Int J Mol Sci. 23:. PubMed
  155. Guo HZ, et al. 2021. Sci Adv. 7:eabg4167. PubMed
  156. Potluri HK, et al. 2022. J Immunother Cancer. 10:. PubMed
  157. Onodera T, et al. 2015. Sci Rep. 5:16801. PubMed
  158. Zhao J, et al. 2021. Front Immunol. 12:658420. PubMed
  159. Moser B, et al. 2021. Mol Cancer. 20:16. PubMed
  160. Hawila E, et al. 2017. Cell Rep.. 10.1016/j.celrep.2017.10.104. PubMed
  161. Wang Y, et al. 2021. Sci Rep. 1.429861111. PubMed
  162. Zhou L, et al. 2020. Clin Cancer Res. 26:290. PubMed
  163. Sertori R, et al. 2022. Front Immunol. 13:928252. PubMed
  164. Lv M, et al. 2020. Cell Res. 30:966. PubMed
  165. Codo AC, et al. 2020. Cell Metab. 32:437. PubMed
  166. Lerrer S, et al. 2021. iScience. 24:103020. PubMed
  167. Di Buduo CA, et al. 2021. eLife. 10:00. PubMed
  168. Lu Z, et al. 2022. Nat Commun. 13:6623. PubMed
  169. Yang Q, et al. 2020. Theranostics. 6.2375. PubMed
  170. Stinson WA, et al. 2022. JCI Insight. 7:. PubMed
  171. Holokai L, et al. 2020. Cancers (Basel). 12:00. PubMed
  172. Monteran L, et al. 2022. Nat Commun. 13:5797. PubMed
  173. Stirling ER, et al. 2022. J Immunother Cancer. 10:. PubMed
  174. Krueger CC, et al. 2019. Front Immunol. 10:1831. PubMed
  175. Shifrut E et al. 2018. Cell. 175(7):1958-1971 . PubMed
  176. Wei C, et al. 2016. Cell Death Dis. 7:e2489. PubMed
  177. Luo Q, et al. 2020. Cancer Sci. 111:4000. PubMed
  178. Tian M, et al. 2021. Elife. 10:. PubMed
  179. Kanemaru H, et al. 2021. iScience. 24:103067. PubMed
  180. Rodriguez RM, et al. 2019. Cell Rep. 29:860. PubMed
  181. Ho JY, et al. 2021. Mol Ther Methods Clin Dev. 21:237. PubMed
  182. Fassler M, et al. 2021. J Neuroinflammation. 18:19. PubMed
  183. Beghelli D, et al. 2017. Oxid Med Cell Longev. 10.1155/2017/7468064. PubMed
  184. Burand AJ, et al. 2020. Front Immunol. 11:143. PubMed
  185. Clement CC, et al. 2021. Immunity. 54:721. PubMed
  186. Feng Y, et al. 2022. Life (Basel). 12:. PubMed
  187. Kwiecinski JM, et al. 2021. Cell Rep. 36:109462. PubMed
  188. Nenasheva T, et al. 2017. PLoS One. 12(6):e0178983. PubMed
  189. Mercer F, et al. 2016. PLoS Negl Trop Dis. 10: 0004913. PubMed
  190. Xie X, et al. 2021. Front Immunol. 12:625808. PubMed
  191. Wettersten HI, et al. 2019. Cancer Res. 79:5048. PubMed
  192. Brockman QR, et al. 2022. JCI Insight. :. PubMed
  193. Lee SH, et al. 2022. Nat Commun. 13:5461. PubMed
  194. Kwee BJ, et al. 2019. Sci Adv. 5:eaav6313. PubMed
  195. Pires D, et al. 2021. Front Immunol. 12:647728. PubMed
  196. Chang T, et al. 2020. Aging (Albany NY). 12:21147. PubMed
  197. Lim J, et al. 2020. Sci Adv. 6:eaba1334. PubMed
  198. Chen W, et al. 2019. Arterioscler Thromb Vasc Biol. 39:2028. PubMed
  199. Montel‐Hagen A et al. 2019. Cell stem cell. 24(3):376-389 . PubMed
  200. Zheng Z, et al. 2021. Biomed Res Int. 2021:5535578. PubMed
  201. Konda P, et al. 2022. Am J Cancer Res. 12:210. PubMed
  202. Soloff A, et al. 2017. J Appl Toxicol. 10.1002/jat.3465. PubMed
  203. Sen S, et al. 2018. J Immunol. 201:440. PubMed
  204. Sung J, et al. 2015. J Infect Dis. 212: 258 - 263. PubMed
  205. Georgouli M et al. 2019. Cell. 176(4):757-774 . PubMed
  206. Rossnagl S, et al. 2016. PLoS Biol. 14: 1002562. PubMed
  207. Lhuillier C, et al. 2021. J Clin Invest. 131:. PubMed
  208. Wang N, et al. 2020. Front Immunol. 1.765972222. PubMed
  209. Zhang Z, et al. 2021. Front Immunol. 12:699478. PubMed
  210. Kwantwi LB, et al. 2021. Bioengineered. 12:6996. PubMed
  211. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
  212. Barsheshet Y, et al. 2017. Proc Natl Acad Sci U S A. 114:6086. PubMed
  213. Ward D, et al. 2016. Haematologica. 101: 286 - 296. PubMed
  214. Cao M, et al. 2021. Int J Cancer. 149:460. PubMed
  215. Ershaid N, et al. 2019. Nat Commun. 10:4375. PubMed
  216. Bonaccorsi-Riani E, et al. 2015. PLoS One. 10: 0136106. PubMed

Antigen Details

Biology Area
Cell Biology, Cell Proliferation and Viability, Neuroscience
Antigen References

1. Parish CR, et al. 2009. Curr. Protoc. Immunol. Unit4.9.
2. Lyons AB, 2000. J. Immunol. Methods 243:147.

Gene ID
NA

Related FAQs

Can I use common compensation control for GFP, CFSE and FITC because they emit in the same channel?
It is not recommended even if they emit in the same channel because these are still different fluors with different brightness intensities. Individual compensation controls should be employed.
Go To Top Version: 5    Revision Date: 05/20/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

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Pricing & Availability
Regulatory Status
RUO
Other Names
5-(and 6)-Carboxyfluorescein diacetate succinimidyl ester Kit, CFDA SE Kit
Ave. Rating
Submit a Review
Product Citations
publications
CFSE_Cell_Tracking_Kit_022014
Human peripheral blood mononuclear cells were stained with CFSE Cell Division Tracking Kit, and then stimulated with (filled histogram) or without (open histogram) PHA for 5 days. On day 5, cells were harvested and the CFSE fluorescent staining was analyzed by flow cytometry.
  • CFSE_Cell_Tracking_Kit_022014
    Human peripheral blood mononuclear cells were stained with CFSE Cell Division Tracking Kit, and then stimulated with (filled histogram) or without (open histogram) PHA for 5 days. On day 5, cells were harvested and the CFSE fluorescent staining was analyzed by flow cytometry.
Cat # Size Price Quantity Check Availability Save
423801 1 kit DKK600
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CFSE Cell Division Tracker Kit is composed of 5 vials, 100 µg per vial of CFSE (formally known as 5-(and 6)-Carboxyfluorescein diacetate succinimidyl ester of CFDA SE), and 500 µl of anhydrous DMSO. CFSE is able to passively diffuse into cells. Inside the cell, its acetate groups are cleaved by intracellular esterases, and the molecules are converted to fluorescent esters. CFSE is retained within the cell and covalently couples to intracellular molecules via its succinimidyl group. Due to this covalent coupling reaction, fluorescent CFSE can be retained within the cell for an extremely long period. Also, due to this stable linkage, once the dye has been incorporated within the cell, it is not transferred to adjacent cells. CFSE is widely used for cell proliferation assays and in vivo cell tracking.

Product Details
Technical Data Sheet (pdf)

Product Details

Preparation
The CFSE Cell Division Tracker Kit is composed of lyophilized CFSE and anhydrous DMSO. For reconstitution, bring the kit to room temperature; add 36 µl of DMSO to one vial of CFSE dye until fully dissolved.
Storage & Handling
Store CFSE Cell Division Tracker Kit at -20°C upon receipt. Do not open vials until needed. Once the DMSO is added to the CFSE, use immediately, or store at -20°C in a dry place and protected from light, preferably in a dessicator or in a container with desiccant for no more than one month.
Application

ICFC - Quality tested
In vivo cell tracking1 - Reported in the literature, not verified in house
Recommended Usage

This lot has been tested by flow cytometric analysis of in vitro cell proliferation assay. It can be used at concentrations ranging from 0.5 - 10 µM for cell labeling. It is recommended that the reagent be titrated for optimal performance for each cell type, culturing condition, or application.

Application Notes

The molecular weight of CFSE is 557.47. The excitation and emission wavelengths of CFSE-labeled cells are 492 nm and 517 nm, respectively. Each 100 µg vial of CFSE may be reconstituted with 36 µl of anhydrous DMSO to yield a stock concentration of 5 mM.

Materials Provided:
5 vials x 100 µg CFSE
500 µl anhydrous DMSO

CFSE Labeling Procedure: 
1. Prior to reconstitution, spin down the vial of lyophilized reagent in a microcentrofuge to ensure the reagent is at the bottom of the vial.
2. Prepare stock solution by reconstituting 1 vial of lyophilized CFSE dye in 36 µL of DMSO to make a 5 mM solution.
3. Prepare a 5 µM working solution by diluting 1 µL of 5 mM CFSE stock solution in 1 mL PBS for every 1 mL of cell suspension (or at an optimal working concentration as determined by titration).
4. Spin down and resuspend cells at 10-100 x 106 cells/mL in the CFSE working solution.
5. Incubate cells for 20 minutes at room temperature or 37°C and keep protected from light.
6. Quench the staining by adding 5 times the original staining volume of cell culture medium containing 10% FBS.
7. Pellet cells and resuspend in pre-warmed cell culture medium. 
8. Incubate cells for 10 minutes.
9. After incubation, CFSE labeled cells are ready for downstream applications or analysis.

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.


Watch a Scientific Poster video of this product.

Application References
  1. Parish CR, et al. 2009. Curr. Protoc. Immunol. Unit4.9.
  2. Miller MJ, et al. 2002. Science 296:1869.
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Antigen Details

Biology Area
Cell Biology, Cell Proliferation and Viability, Neuroscience
Antigen References

1. Parish CR, et al. 2009. Curr. Protoc. Immunol. Unit4.9.
2. Lyons AB, 2000. J. Immunol. Methods 243:147.

Gene ID
NA

Related FAQs

Can I use common compensation control for GFP, CFSE and FITC because they emit in the same channel?
It is not recommended even if they emit in the same channel because these are still different fluors with different brightness intensities. Individual compensation controls should be employed.
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