Loading...

FITC Annexin V FITC Annexin V

Pricing & Availability
Regulatory Status
RUO
Other Names
Annexin A5, Dead, Apoptosis
Ave. Rating
Submit a Review
Product Citations
publications
AnnexinV_FITC_092908.jpg
Human T leukemia cell line, Jurkat, non-treated (top) or treated (bottom) with BioLegend’s anti-human CD95 (EOS9.1) mAb (cat. 305704) for 6 hours, then stained with Annexin V-FITC and Propidium Iodide (PI) (cat. 421301)
  • AnnexinV_FITC_092908.jpg
    Human T leukemia cell line, Jurkat, non-treated (top) or treated (bottom) with BioLegend’s anti-human CD95 (EOS9.1) mAb (cat. 305704) for 6 hours, then stained with Annexin V-FITC and Propidium Iodide (PI) (cat. 421301)
  • B_AnnexinV_FITC_092908.jpg
See FITC spectral data
Cat # Size Price Quantity Check Availability Save
640905 25 tests DKK494
Check Availability


Need larger quantities of this item?
Request Bulk Quote
640906 100 tests DKK1153
Check Availability


Need larger quantities of this item?
Request Bulk Quote
640945 300 tests DKK2406
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry is lost and PS translocates to the external leaflet. Fluorochrome-labeled Annexin V can then be used to specifically target and identify apoptotic cells. Annexin V Binding Buffer (cat. no. 422201) is recommended for use with Annexin V staining.Annexin V binding alone cannot differentiate between apoptotic cells and necrotic. Therefore, we recommend using our Helix NP™ Blue (Cat. No. 425305), Helix NP™ Green (Cat.  No. 425303) or Helix NP™ NIR (Cat. No. 425301). Early apoptotic cells will exclude 7-AAD and PI, while late stage apoptotic cells and necrotic cells will stain positively, due to the passage of these dyes into the nucleus where they bind to DNA.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Reported Reactivity
Other Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The purified protein was conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The Annexin V solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this product is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per 100,000 - million cells in a 100 µl volume of Annexin V Binding Buffer (Cat No. 422201). It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Annexin V Staining

  1. Wash cells twice with cold BioLegend Cell Staining Buffer (Cat. No. 420201) and then resuspend cells in Annexin V Binding Buffer (Cat. No. 422201) at a concentration of 1x106 cells/mL.
  2. Transfer 100 µL of cell suspension in 5 mL test tube.
  3. Add 5 µL of fluorochrome conjugated Annexin V.
  4. Stain with a viability dye, such as PI (Cat. No. 421301), 7-AAD (Cat. Nos. 420403 & 420404), or Helix NP dyes (Cat. Nos. 425301, 425303, & 425305), if desired.
  5. Gently vortex the cells and incubate for 15 min at RT (25°C) in the dark.
  6. Add 400* µL of Annexin V Binding Buffer (Cat. No. 422201) to each tube. *For more concentrated samples, add a minimum of 200 µl of Annexin V Binding Buffer in this step.
  7. Analyze by flow cytometry.

For a better experience detecting apoptosis, we now recommend Apotracker™. Cell staining with Apotracker™ is Calcium independent. Thus, no special buffers are required, and the protocol can be shortened for single-step co-staining with other reagents.

Application References
  1. Koopman G, et al. 1994. Blood 84:1415.
  2. Vermes I, et al. 1995. J. Immunol. Methods 184:39.
  3. Dachary-Prigent J, et al. 1993. Blood 81:2554.
  4. Sekine C, et al. 2009. Int Immunol. PubMed
  5. Grujic M, et al. 2010. J. Immunol. 185:1730. PubMed
  6. Mulik RS, et al. 2010. Int J Pharm. 398:190. PubMed
  7. Gupta A, et al. 2011. Leuk Res. 35:1498. PubMed
  8. Speth C,et al. 2013. J Infect Dis. 207:823. PubMed
  9. Wang Q, et al. 2013. Genes Dev. 27:615. PubMed
  10. Gill K, et al. 2013. Biocheim Biophys Acta. 830:2763. PubMed
  11. Juel HB, et al. 2013. PLoS One. 8:64619. PubMed
  12. Hellmann J, et al. 2013. J. Immunol. 191:1383. PubMed
  13. Sequier S, et al. 2013. PLoS One. 8:70937. PubMed
  14. Galski H, et al. 2013. Biochem Pharmacol. 86:584. PubMed
  15. Chen YL, et al. 2013. J Exp Med. PubMed
  16. Lin WH, et al. 2014. PLoS One. 9:83160. PubMed
  17. Salge-Bartles U, 2014. Thromb res. 133:244. PubMed
  18. Zhang X, et al. 2014. Acta Biochim Biophys Sin. 46:261. PubMed
  19. Intermann J, et al. 2014. Cell Signal. 26:1567. PubMed
  20. de Vires M, 2014. Am J Physiol Lung Cell Mol Physiol. 307:240. PubMed
  21. M Parrula MC, et al. 2014. Virus Res. 189:206. PubMed
  22. Passaro C, et al. 2014. Mol Oncol. 9:78. PubMed
  23. Tsou WI, et al. 2014. J Biol Chem. 289:25750. PubMed
  24. Awojoodu AO, et al. 2014. Blood. 124:1941. PubMed
  25. Xiong R, et al. 2014. Toxicol Appl Pharmacol. 280:285. PubMed
  26. Liu J, et al. 2015. J Mol Cell Cardiol. 80:10. PubMed
Product Citations
  1. Uriostegui–Arcos M, et al. 2020. Open Biol. 10:200050. PubMed
  2. Zhang T, et al. 2022. EBioMedicine. 77:103872. PubMed
  3. Dhoonmoon A, et al. 2022. Nat Commun. 13:5063. PubMed
  4. Yao Z, et al. 2018. J Virol. 92:00:00. PubMed
  5. Glynn RA, et al. 2022. J Immunol. 208:371. PubMed
  6. Cao H, et al. 2022. Br J Haematol. 198:155. PubMed
  7. Ugamraj HS, et al. 2022. MAbs. 14:2095949. PubMed
  8. Cao L, et al. 2022. Cell Rep. 40:111156. PubMed
  9. Choi UY, et al. 2022. Cell Rep. 40:111234. PubMed
  10. Kimmerling RJ, et al. 2022. Commun Biol. 5:1295. PubMed
  11. Chen X, et al. 2022. Front Pharmacol. 13:1028692. PubMed
  12. Shahid AM, et al. 2022. PLoS One. 17:e0278209. PubMed
  13. Önel T, et al. 2023. Naunyn Schmiedebergs Arch Pharmacol. :. PubMed
  14. Papakonstantinou A, et al. 2022. Int J Mol Sci. 24: . PubMed
  15. Li J, et al. 2023. J Biol Chem. 299:102875. PubMed
  16. Martinelli M, et al. 2023. iScience. 26:105860. PubMed
  17. Tay HG, et al. 2023. Mol Ther. 31:825. PubMed
  18. Dean BD, et al. 2023. J Immunother Cancer. 11: . PubMed
  19. Lieschke E, et al. 2022. Cell Death Differ. 29:1004. PubMed
  20. Gallego-Murillo JS, et al. 2022. Biotechnol Bioeng. 119:3096. PubMed
  21. Du X, et al. 2023. Nucleic Acids Res. 51:1050. PubMed
  22. Vanoli F, et al. 2023. Oncogenesis. 12:8. PubMed
  23. Lei K, et al. 2023. iScience. 26:106001. PubMed
  24. Ohuchi K, et al. 2023. Biomed Rep. 18:28. PubMed
  25. Do-Thi VA, et al. 2023. Cancer Res Commun. 3:80. PubMed
  26. Perez GI, et al. 2023. Vaccines (Basel). 11:. PubMed
  27. Segaliny AI, et al. 2023. Commun Biol. 6:380. PubMed
  28. Potez M, et al. 2023. Front Oncol. 13:1124272. PubMed
  29. Woodley K, et al. 2023. Nat Commun. 14:2132. PubMed
  30. Quintela M, et al. 2023. Clin Epigenetics. 15:63. PubMed
  31. Quintela M, et al. 2023. Br J Cancer. . PubMed
  32. Zelenka T, et al. 2022. Nat Commun. 13:6954. PubMed
  33. Derudder E, et al. 2016. Proc Natl Acad Sci U S A. 113: 5065-5070. PubMed
  34. Dowling CM, et al. 2021. Sci Adv. 7:. PubMed
  35. Lin YN, et al. 2022. Oncoimmunology. 11:2027136. PubMed
  36. Kim EH, et al. 2020. Elife. 9:00. PubMed
  37. Bouhaddou M, et al. 2018. PLoS Comput Biol. 14:e1005985. PubMed
  38. Espinosa-Cueto P, et al. 2017. PLoS One.. 10.1371/journal.pone.0182126. PubMed
  39. Kitayama J, et al. 2016. PLoS One. 11: 0154542. PubMed
  40. Gedye C, et al. 2016. Sci Rep. 6: 25220. PubMed
  41. Carlsson J, et al. 2015. PLoS One. 10: 0143741. PubMed
  42. Wang Q, et al. 2022. Nat Commun. 13:3022. PubMed
  43. Alcon C, et al. 2020. Cell Death Dis. 0.898611111. PubMed
  44. Figueiredo T, et al. 2020. Mol Psychiatry. . PubMed
  45. Lopes-Coelho F, et al. 2021. Front Oncol. 11:656229. PubMed
  46. Yamauchi T et al. 2018. Cancer cell. 33(3):386-400 . PubMed
  47. Feng W, et al. 2017. Nat Commun. 0.697916667. PubMed
  48. Jones PD, et al. 2018. Arterioscler Thromb Vasc Biol. 38:1711. PubMed
  49. Olelewe C, et al. 2022. iScience. 25:104340. PubMed
  50. Gupta A, et al. 2011. Leuk Res. 35:1498. PubMed
  51. Fujii T, et al. 2015. Proc Natl Acad Sci U S A. 112: 12800 - 12805. PubMed
  52. Cardoso B, et al. 2015. PLoS One. 10: 0143897. PubMed
  53. Bowen ME, et al. 2019. Dev Cell. 50:212. PubMed
  54. Sales–Dias J, et al. 2019. PLoS One. 14:e0217002. PubMed
  55. Santos LC, et al. 2019. Nat Commun. 1.328472222. PubMed
  56. Kim Y, et al. 2020. J Neurochem. 155:177. PubMed
  57. Hassan MIA, et al. 2021. Comput Struct Biotechnol J. 19:880. PubMed
  58. Ramalingam P, et al. 2020. J Exp Med. 217:00:00. PubMed
  59. Lim JA, et al. 2018. BMC Cancer. 0.860416667. PubMed
  60. Venter C, et al. 2017. Cell Physiol Biochem. 43:1288. PubMed
  61. Fortin J, et al. 2020. Cancer Cell. 37:308. PubMed
  62. Vereertbrugghen A, et al. 2021. Front Oncol. 11:598319. PubMed
  63. Sun V, et al. 2021. Front Immunol. 12:716661. PubMed
  64. Macpherson L, et al. 2020. Nature. 577:266. PubMed
  65. Karavasili C, et al. 2017. International Journal of Pharmaceutics. 10.1016/j.ijpharm.2017.05.061. PubMed
  66. Thompson PJ et al. 2019. Cell metabolism. 29(5):1045-1060 . PubMed
  67. Noguchi F, et al. 2017. Mol Cancer Ther. 1.316666667. PubMed
  68. Kumar S, et al. 2018. Cancers (Basel). 0.724305556. PubMed
  69. Coray M, et al. 2022. Int J Mol Sci. 23:. PubMed
  70. Soprano M, et al. 2016. PLoS One. 11: 0155970. PubMed
  71. Chen MS, et al. 2019. Mol Cancer Res. 17:697. PubMed
  72. Kim OV, et al. 2022. iScience. 25:104654. PubMed
  73. Shahzadi SK, et al. 2022. Clin Sci (Lond). 136:181. PubMed
  74. Kolbrink B, et al. 2022. Biochem J. 479:259. PubMed
  75. He C, et al. 2021. Oncoimmunology. 1897295:10. PubMed
  76. Ni F, et al. 2019. Cell Stem Cell. 24:608. PubMed
  77. Ning X, et al. 2019. Mol Cell. 74:19. PubMed
  78. Greenblatt SM, et al. 2018. Cancer Cell. 33:1111. PubMed
  79. Naqvi N,et al. 2017. Sci Rep.. 10.1038/s41598-017-12753-1. PubMed
  80. Wang M, et al. 2017. Biochemical and Biophysical Research Communications. 10.1016/j.bbrc.2017.05.097. PubMed
  81. Beeravolu N, McKee C, Chaudhry G 2017. J App Toxicol. 10.1002/jat.3469. PubMed
  82. Pfalzgraff A, et al. 2016. Sci Rep. 6:31577. PubMed
  83. O'Neill K, et al. 2016. Genes Dev. 30: 973-988. PubMed
  84. Liu J, et al. 2015. J Mol Cell Cardiol. 80:10. PubMed
  85. Vries M, et al. 2014. Am J Physiol Lung Cell Mol Physiol. 307:240. PubMed
  86. Esteghamat F, et al. 2019. Nat Genet. 51:1233. PubMed
  87. Blümel E, et al. 2020. Oncoimmunology. 9:1751561. PubMed
  88. Sekine C, et al. 2009. Int Immunol. 1.079861111. PubMed
  89. Kinsella S, et al. 2021. Cell Rep. 37:109789. PubMed
  90. O'Connor MJ, et al. 2021. Oncogene. 40:4872. PubMed
  91. Parrula M, et al. 2014. Virus Res. 189:206. PubMed
  92. Brigger D, et al. 2015. Cell Death Dis. 6: e1861. PubMed
  93. Zhang J, et al. 2016. Cell Death Dis. 7:e2266. PubMed
  94. Wang D, et al. 2016. J Cell Sci. 129: 2261 - 2272. PubMed
  95. Uchtenhagen H, et al. 2016. Nat Commun. 7:12614. PubMed
  96. Geiger R, et al. 2016. Cell. 167:829-842. PubMed
  97. Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
  98. Martín Monreal MT, et al. 2021. Front Immunol. 12:716250. PubMed
  99. Schmitz RL, et al. 2021. Cells. 10:. PubMed
  100. Babaei A, et al. 2021. Adv Pharm Bull. 11:361. PubMed
  101. Madala HR, et al. 2020. Cancer Res. 3492:80. PubMed
  102. Clements KE, et al. 2020. Nat Commun. 4.706944444. PubMed
  103. Rauzan M, et al. 2017. PLoS One. 12:e0174107. PubMed
  104. Liu Y, et al. 2017. Acta Biomater. 10.1016/j.actbio.2017.08.021. PubMed
  105. Chiani M, et al. 2017. Artif Cells Nanomed Biotechnol. 10.1080/21691401.2017.1337029. PubMed
  106. Blümel E, et al. 2019. Oncoimmunology. 8:e1641387. PubMed
  107. Krauss PL, et al. 2021. Front Immunol. 12:730672. PubMed
  108. Herold N, et al. 2017. Nat Med. 23:256-263. PubMed
  109. Xie S, et al. 2021. Sci Rep. 0.660416667. PubMed
  110. Müller I, et al. 2020. Mol Cell. 970:77. PubMed
  111. Morimoto A, et al. 2021. Nat Commun. 12:2136. PubMed
  112. Norkin M, et al. 2021. Cell Rep. 35:109026. PubMed
  113. Kamiyama N, et al. 2017. Antiviral Res. 10.1016/j.antiviral.2017.08.007. PubMed
  114. Montero–Herradón S, et al. 2017. Organogenesis. 0.675. PubMed
  115. Nunes SC, et al. 2018. Sci Rep. 6.939583333. PubMed
  116. Sharif T, et al. 2018. Autophagy. 1.101388889. PubMed
  117. Eichelmann AK, et al. 2018. Sci Rep. 8:17553. PubMed
  118. Zhu Y, et al. 2022. Clin Transl Med. 12:e887. PubMed
  119. Khatib JB, et al. 2022. Oncotarget. 13:1078. PubMed
  120. Salge-Bartels U, et al. 2014. Thromb Res. 133:244. PubMed
  121. Passaro C, et al. 2014. Mol Oncol. 9:78. PubMed
  122. Ligtenberg M, et al. 2016. J Immunol. 196: 759 - 766. PubMed
  123. Hrdinka M, et al. 2016. PLoS One. 11: 0162863. PubMed
  124. Liu H, et al. 2020. Cell Death Dis. 0.767361111. PubMed
  125. Vanoli F, et al. 2021. Oncogene. 40:5095. PubMed
  126. Liu H, et al. 2021. J Immunother Cancer. 9:. PubMed
  127. Smith CM, et al. 2021. Biochem J. 478:3331. PubMed
  128. Li Y, et al. 2021. Sci Transl Med. 13:. PubMed
  129. Meyer N, et al. 2021. J Clin Med. 10:. PubMed
  130. Zervou S, et al. 2017. PLoS One.. 10.1371/journal.pone.0182994. PubMed
  131. Ishidome T et al. 2017. EBioMedicine. 22:89-99 . PubMed
  132. Matsuda K, et al. 2021. Cell Rep Methods. 1:100122. PubMed
  133. Yoon Y, et al. 2016. Stem Cell Reports. 7:840-853. PubMed
  134. Chheda Z, et al. 2016. J Immunol. 197: 2016 - 2026. PubMed
  135. Slone E, et al. 2015. J Leukoc Biol. 98: 791 - 804. PubMed
  136. Séguier S, et al. 2013. PLoS One. 8:70937. PubMed
  137. Grujic M, et al. 2010. J Immunol. 185:1730. PubMed
  138. Wang Q, et al. 2013. Genes Dev. 27:615. PubMed
  139. Meyer N, et al. 2021. Int J Mol Sci. 22:. PubMed
  140. Kolbrink B, et al. 2022. Cell Mol Life Sci. 79:387. PubMed
  141. Pajarillo E, et al. 2020. J Biol Chem. 295:3040. PubMed
  142. Gholamrezaei M, et al. 2021. Iran J Parasitol. 15:475. PubMed
  143. Litzenburger UM, et al. 2017. Genome Biol. 18:15. PubMed
  144. Rajavel T, et al. 2017. Sci Rep. 2.665277778. PubMed
  145. Douguet L, et al. 2016. PLoS One. 11:e0166164. PubMed
  146. Toutounchian J, et al. 2016. Mol Pharmacol. 91(1):1-13. PubMed
  147. Matsumoto A, et al. 2019. J Extracell Vesicles. 9:1696517. PubMed
  148. Ishida M, et al. 2021. Int J Mol Sci. 22:. PubMed
  149. Lee DH, et al. 2020. Circ Res. 127:1182. PubMed
  150. Alsina-Sanchis E, et al. 2020. Mol Cancer Res. . PubMed
  151. Pirali T, et al. 2017. ChemMedChem. 1.570833333. PubMed
  152. L Chang, Y Li, D Kaplan 2016. Sci Rep. 6:36862. PubMed
  153. Becker A, et al. 2016. Sci Rep. 6: 33847. PubMed
  154. Kumai T, et al. 2015. Sci Rep. 5:16280. PubMed
  155. Polak R, et al. 2015. Blood. 126: 2404 - 2414. PubMed
  156. Putheti P, et al. 2010. PLoS One. 5:e8706. PubMed
  157. Molinar-Inglis O, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  158. Dhoonmoon A, et al. 2020. Nucleic Acids Res. 48:7252. PubMed
  159. Nastasi C, et al. 2021. Sci Rep. 11:1458. PubMed
  160. Hafez A, et al. 2017. Oncogenesis. 10.1038/oncsis.2017.46. PubMed
  161. Erdogan M, et al. 2022. Chem Biodivers. 19:e202200659. PubMed
  162. Galski H, et al. 2013. Biochem Pharmacol. 86:584. PubMed
  163. Tsou W, et al. 2014. J Biol Chem. 289:25750. PubMed
  164. Speth C, et al. 2013. J Infect Dis. 207:823. PubMed
  165. Gladow N, et al. 2020. PLoS One. 15:e0227734. PubMed
  166. Shadboorestan A, et al. 2022. Iran J Basic Med Sci. 25:506. PubMed
  167. Huang Y, et al. 2022. Front Cell Dev Biol. 9:837777. PubMed
  168. Babaei A, et al. 2020. Daru. 28:555. PubMed
  169. King R, et al. 2021. Sci Adv. 7:eabj5293. PubMed
  170. Cai Z, et al. 2020. Cell Rep. 31:107816. PubMed
  171. Lee HW, et al. 2018. Am J Physiol Renal Physiol. 314:F280. PubMed
  172. Ki H, et al. 2017. Biomed Pharmacother. 10.1016/j.biopha.2017.09.118. PubMed
  173. Liu B, et al. 2016. J Biol Chem. 291: 23869 - 23881. PubMed
  174. Lhuillier C, et al. 2015. J Biol Chem. 290: 16797 - 16811. PubMed
  175. Passaro C, et al. 2013. Endocr Relat Cancer. 20:633. PubMed
  176. Mulik R, et al. 2010. Int J Pharm. 398:190. PubMed
  177. Di Somma S, et al. 2019. Cancers (Basel). 1.70625. PubMed
  178. Jackson LM, et al. 2021. Nucleic Acids Res. 49:12855. PubMed
  179. Huang YL, et al. 2020. Elife. 9:00. PubMed
  180. Abu El Maaty MA, et al. 2021. Sci Adv. 7: . PubMed
  181. Bahl S, et al. 2021. Cell Death Dis. 12:469. PubMed
  182. Schuler F, et al. 2017. Nat Commun. . 10.1038/s41467-017-01850-4. PubMed
  183. Lin W, et al. 2014. PLoS One. 9:83160. PubMed
  184. Zhang X, et al. 2014. Acta Biochim Biophys Sin. 46:261. PubMed
  185. CO L, et al. 2015. Nat Commun. 6: 7739. PubMed
  186. Labi V, et al. 2019. Genes Dev. 33:1673. PubMed
  187. Fang P, et al. 2019. Commun Biol. 0.315972222. PubMed
  188. Kumar S, et al. 2019. Int J Cancer. 146:1652. PubMed
  189. Tatangelo V, et al. 2022. Front Oncol. 12:877495. PubMed
  190. Alcon C, et al. 2021. Cells. 10:. PubMed
  191. Cao H, et al. 2021. Nat Commun. 12:1792. PubMed
  192. Faria M, et al. 2017. PLoS One. 12(2):e0172689. PubMed
  193. Zhao CC, et al. 2018. Oncol Rep. 40:3392. PubMed
  194. Schleicher EM, et al. 2018. Nucleic Acids Res. 46:8908. PubMed
  195. Thomas A, et al. 2017. PLoS One.. 10.1371/journal.pone.0183542. PubMed
  196. Jones P, et al. 2016. J Biol Chem. 291: 16318 - 16327. PubMed
  197. Awojoodu A, et al. 2014. Blood. 124:1941. PubMed
  198. Intemann J, et al. 2014. Cell Signal. 26:1567. PubMed
  199. Monzen S, et al. 2022. Mol Clin Oncol. 16:19. PubMed
  200. Konda P, et al. 2022. Am J Cancer Res. 12:210. PubMed
  201. Wu J, et al. 2021. Adv Sci (Weinh). 8:e2101029. PubMed
  202. Bostanabad SY, et al. 2021. Sci Rep. 11:1539. PubMed
  203. Clements KE, et al. 2018. Nucleic Acids Res. 46:8898. PubMed
  204. Mendès C, et al. 2019. Sci Rep. 9:14107. PubMed
  205. Tu Z, et al. 2022. Cell Rep. 40:111072. PubMed
  206. Bensberg M, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  207. Paparella AS, et al. 2021. Nat Commun. 12:6285. PubMed
  208. Huang YL, et al. 2021. iScience. 24:103168. PubMed
  209. Zhang Y, et al. 2021. Cell Death Dis. 0.563194444. PubMed
  210. Kang JH, et al. 2020. Cell Death Dis. 0.803472222. PubMed
  211. Dewdney B, et al. 2020. Sci Rep. 10:16769. PubMed
  212. Simoni L, et al. 2020. Cell Rep. 33:108330. PubMed
  213. Lopes–Coelho F, et al. 2017. Oncotarget. 8:82803. PubMed
  214. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  215. Thanuthanakhun N, et al. 2017. PLoS One. . 10.1371/journal.pone.0187610. PubMed
  216. Kuntz E, et al. 2017. Nat Med. 10.1038/nm.4399. PubMed
  217. Coutaz M, et al. 2016. Sci Rep. 6:39117. PubMed
  218. Akane K, et al. 2016. Proc Natl Acad Sci U S A. 113: 2460 - 2465. PubMed
  219. Xiong R, et al. 2014. Toxicol Appl Pharmacol. 280:285. PubMed
  220. Chen Y, et al. 2013. J Exp Med. 210:2515. PubMed
  221. Lyu X, et al. 2019. BMC Genomics. 1.235416667. PubMed
  222. Jing Y, et al. 2019. J Allergy Clin Immunol. 144:1377. PubMed
  223. Quan M, et al. 2019. Oncol Rep. 41:3051. PubMed
  224. Kasikara C, et al. 2021. J Clin Invest. 131:. PubMed
  225. Ning N, et al. 2022. Theranostics. 12:5537. PubMed
  226. Bottardi S, et al. 2020. Exp Hematol. 68:88. PubMed
  227. Siddhartha V, et al. 2017. Artif Cells Nanomed Biotechnol . 10.1080/21691401.2017.1313267. PubMed
  228. He J, et al. 2017. FASEB J. 31:2893. PubMed
  229. Juel H, et al. 2013. PLoS One. 8:64619. PubMed
  230. Gill K, et al. 2013. Biochim Biophys Acta. 1830:2763. PubMed
  231. Young MM, et al. 2019. Cell Death Dis. 1.004861111. PubMed
  232. Santos I, et al. 2019. Nutrients. 2.210416667. PubMed
  233. Vancsik T, et al. 2019. Cancer Med. 3.313888889. PubMed
  234. Zheng M, et al. 2019. Proc Natl Acad Sci U S A. 116:12422. PubMed
  235. Shankman LS, et al. 2021. Curr Biol. 31:2469. PubMed
  236. Du Z, et al. 2020. J Allergy Clin Immunol. . PubMed
  237. Pahari S, et al. 2020. Autophagy. 1.375694444. PubMed
  238. Schleicher EM, et al. 2020. PLoS Genet. 16:e1009176. PubMed
  239. Zheng Q, et al. 2017. Mol Oncol. 10.1002/1878-0261.12056. PubMed
  240. Wang Y, et al. 2022. Front Cardiovasc Med. 9:876087. PubMed
  241. Langsch S, et al. 2016. Cancer Res . 76: 4160 - 4169. PubMed
  242. Xiong R, et al. 2015. Mol Pharmacol. 88: 1045 - 1054. PubMed
  243. Hellmann J, et al. 2013. J Immunol. 191:1383. PubMed
  244. Uriostegui–Arcos M, et al. 2020. Open Biol. 10:200050. PubMed
  245. Zhang T, et al. 2022. EBioMedicine. 77:103872. PubMed
  246. Dhoonmoon A, et al. 2022. Nat Commun. 13:5063. PubMed
  247. Yao Z, et al. 2018. J Virol. 92:00:00. PubMed
  248. Glynn RA, et al. 2022. J Immunol. 208:371. PubMed
  249. Cao H, et al. 2022. Br J Haematol. 198:155. PubMed
  250. Ugamraj HS, et al. 2022. MAbs. 14:2095949. PubMed
  251. Cao L, et al. 2022. Cell Rep. 40:111156. PubMed
  252. Choi UY, et al. 2022. Cell Rep. 40:111234. PubMed
  253. Kimmerling RJ, et al. 2022. Commun Biol. 5:1295. PubMed
  254. Chen X, et al. 2022. Front Pharmacol. 13:1028692. PubMed
  255. Shahid AM, et al. 2022. PLoS One. 17:e0278209. PubMed
  256. Önel T, et al. 2023. Naunyn Schmiedebergs Arch Pharmacol. :. PubMed
  257. Papakonstantinou A, et al. 2022. Int J Mol Sci. 24: . PubMed
  258. Li J, et al. 2023. J Biol Chem. 299:102875. PubMed
  259. Martinelli M, et al. 2023. iScience. 26:105860. PubMed
  260. Tay HG, et al. 2023. Mol Ther. 31:825. PubMed
  261. Dean BD, et al. 2023. J Immunother Cancer. 11: . PubMed
  262. Lieschke E, et al. 2022. Cell Death Differ. 29:1004. PubMed
  263. Gallego-Murillo JS, et al. 2022. Biotechnol Bioeng. 119:3096. PubMed
  264. Du X, et al. 2023. Nucleic Acids Res. 51:1050. PubMed
  265. Vanoli F, et al. 2023. Oncogenesis. 12:8. PubMed
  266. Lei K, et al. 2023. iScience. 26:106001. PubMed
  267. Ohuchi K, et al. 2023. Biomed Rep. 18:28. PubMed
  268. Do-Thi VA, et al. 2023. Cancer Res Commun. 3:80. PubMed
  269. Perez GI, et al. 2023. Vaccines (Basel). 11:. PubMed
  270. Segaliny AI, et al. 2023. Commun Biol. 6:380. PubMed
  271. Potez M, et al. 2023. Front Oncol. 13:1124272. PubMed
  272. Woodley K, et al. 2023. Nat Commun. 14:2132. PubMed
  273. Quintela M, et al. 2023. Clin Epigenetics. 15:63. PubMed
  274. Quintela M, et al. 2023. Br J Cancer. . PubMed
  275. Zelenka T, et al. 2022. Nat Commun. 13:6954. PubMed
  276. Derudder E, et al. 2016. Proc Natl Acad Sci U S A. 113: 5065-5070. PubMed
  277. Dowling CM, et al. 2021. Sci Adv. 7:. PubMed
  278. Lin YN, et al. 2022. Oncoimmunology. 11:2027136. PubMed
  279. Kim EH, et al. 2020. Elife. 9:00. PubMed
  280. Bouhaddou M, et al. 2018. PLoS Comput Biol. 14:e1005985. PubMed
  281. Espinosa-Cueto P, et al. 2017. PLoS One.. 10.1371/journal.pone.0182126. PubMed
  282. Kitayama J, et al. 2016. PLoS One. 11: 0154542. PubMed
  283. Gedye C, et al. 2016. Sci Rep. 6: 25220. PubMed
  284. Carlsson J, et al. 2015. PLoS One. 10: 0143741. PubMed
  285. Wang Q, et al. 2022. Nat Commun. 13:3022. PubMed
  286. Alcon C, et al. 2020. Cell Death Dis. 0.898611111. PubMed
  287. Figueiredo T, et al. 2020. Mol Psychiatry. . PubMed
  288. Lopes-Coelho F, et al. 2021. Front Oncol. 11:656229. PubMed
  289. Yamauchi T et al. 2018. Cancer cell. 33(3):386-400 . PubMed
  290. Feng W, et al. 2017. Nat Commun. 0.697916667. PubMed
  291. Jones PD, et al. 2018. Arterioscler Thromb Vasc Biol. 38:1711. PubMed
  292. Olelewe C, et al. 2022. iScience. 25:104340. PubMed
  293. Gupta A, et al. 2011. Leuk Res. 35:1498. PubMed
  294. Fujii T, et al. 2015. Proc Natl Acad Sci U S A. 112: 12800 - 12805. PubMed
  295. Cardoso B, et al. 2015. PLoS One. 10: 0143897. PubMed
  296. Bowen ME, et al. 2019. Dev Cell. 50:212. PubMed
  297. Sales–Dias J, et al. 2019. PLoS One. 14:e0217002. PubMed
  298. Santos LC, et al. 2019. Nat Commun. 1.328472222. PubMed
  299. Kim Y, et al. 2020. J Neurochem. 155:177. PubMed
  300. Hassan MIA, et al. 2021. Comput Struct Biotechnol J. 19:880. PubMed
  301. Ramalingam P, et al. 2020. J Exp Med. 217:00:00. PubMed
  302. Lim JA, et al. 2018. BMC Cancer. 0.860416667. PubMed
  303. Venter C, et al. 2017. Cell Physiol Biochem. 43:1288. PubMed
  304. Fortin J, et al. 2020. Cancer Cell. 37:308. PubMed
  305. Vereertbrugghen A, et al. 2021. Front Oncol. 11:598319. PubMed
  306. Sun V, et al. 2021. Front Immunol. 12:716661. PubMed
  307. Macpherson L, et al. 2020. Nature. 577:266. PubMed
  308. Karavasili C, et al. 2017. International Journal of Pharmaceutics. 10.1016/j.ijpharm.2017.05.061. PubMed
  309. Thompson PJ et al. 2019. Cell metabolism. 29(5):1045-1060 . PubMed
  310. Noguchi F, et al. 2017. Mol Cancer Ther. 1.316666667. PubMed
  311. Kumar S, et al. 2018. Cancers (Basel). 0.724305556. PubMed
  312. Coray M, et al. 2022. Int J Mol Sci. 23:. PubMed
  313. Soprano M, et al. 2016. PLoS One. 11: 0155970. PubMed
  314. Chen MS, et al. 2019. Mol Cancer Res. 17:697. PubMed
  315. Kim OV, et al. 2022. iScience. 25:104654. PubMed
  316. Shahzadi SK, et al. 2022. Clin Sci (Lond). 136:181. PubMed
  317. Kolbrink B, et al. 2022. Biochem J. 479:259. PubMed
  318. He C, et al. 2021. Oncoimmunology. 1897295:10. PubMed
  319. Ni F, et al. 2019. Cell Stem Cell. 24:608. PubMed
  320. Ning X, et al. 2019. Mol Cell. 74:19. PubMed
  321. Greenblatt SM, et al. 2018. Cancer Cell. 33:1111. PubMed
  322. Naqvi N,et al. 2017. Sci Rep.. 10.1038/s41598-017-12753-1. PubMed
  323. Wang M, et al. 2017. Biochemical and Biophysical Research Communications. 10.1016/j.bbrc.2017.05.097. PubMed
  324. Beeravolu N, McKee C, Chaudhry G 2017. J App Toxicol. 10.1002/jat.3469. PubMed
  325. Pfalzgraff A, et al. 2016. Sci Rep. 6:31577. PubMed
  326. O'Neill K, et al. 2016. Genes Dev. 30: 973-988. PubMed
  327. Liu J, et al. 2015. J Mol Cell Cardiol. 80:10. PubMed
  328. Vries M, et al. 2014. Am J Physiol Lung Cell Mol Physiol. 307:240. PubMed
  329. Esteghamat F, et al. 2019. Nat Genet. 51:1233. PubMed
  330. Blümel E, et al. 2020. Oncoimmunology. 9:1751561. PubMed
  331. Sekine C, et al. 2009. Int Immunol. 1.079861111. PubMed
  332. Kinsella S, et al. 2021. Cell Rep. 37:109789. PubMed
  333. O'Connor MJ, et al. 2021. Oncogene. 40:4872. PubMed
  334. Parrula M, et al. 2014. Virus Res. 189:206. PubMed
  335. Brigger D, et al. 2015. Cell Death Dis. 6: e1861. PubMed
  336. Zhang J, et al. 2016. Cell Death Dis. 7:e2266. PubMed
  337. Wang D, et al. 2016. J Cell Sci. 129: 2261 - 2272. PubMed
  338. Uchtenhagen H, et al. 2016. Nat Commun. 7:12614. PubMed
  339. Geiger R, et al. 2016. Cell. 167:829-842. PubMed
  340. Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
  341. Martín Monreal MT, et al. 2021. Front Immunol. 12:716250. PubMed
  342. Schmitz RL, et al. 2021. Cells. 10:. PubMed
  343. Babaei A, et al. 2021. Adv Pharm Bull. 11:361. PubMed
  344. Madala HR, et al. 2020. Cancer Res. 3492:80. PubMed
  345. Clements KE, et al. 2020. Nat Commun. 4.706944444. PubMed
  346. Rauzan M, et al. 2017. PLoS One. 12:e0174107. PubMed
  347. Liu Y, et al. 2017. Acta Biomater. 10.1016/j.actbio.2017.08.021. PubMed
  348. Chiani M, et al. 2017. Artif Cells Nanomed Biotechnol. 10.1080/21691401.2017.1337029. PubMed
  349. Blümel E, et al. 2019. Oncoimmunology. 8:e1641387. PubMed
  350. Krauss PL, et al. 2021. Front Immunol. 12:730672. PubMed
  351. Herold N, et al. 2017. Nat Med. 23:256-263. PubMed
  352. Xie S, et al. 2021. Sci Rep. 0.660416667. PubMed
  353. Müller I, et al. 2020. Mol Cell. 970:77. PubMed
  354. Morimoto A, et al. 2021. Nat Commun. 12:2136. PubMed
  355. Norkin M, et al. 2021. Cell Rep. 35:109026. PubMed
  356. Kamiyama N, et al. 2017. Antiviral Res. 10.1016/j.antiviral.2017.08.007. PubMed
  357. Montero–Herradón S, et al. 2017. Organogenesis. 0.675. PubMed
  358. Nunes SC, et al. 2018. Sci Rep. 6.939583333. PubMed
  359. Sharif T, et al. 2018. Autophagy. 1.101388889. PubMed
  360. Eichelmann AK, et al. 2018. Sci Rep. 8:17553. PubMed
  361. Zhu Y, et al. 2022. Clin Transl Med. 12:e887. PubMed
  362. Khatib JB, et al. 2022. Oncotarget. 13:1078. PubMed
  363. Salge-Bartels U, et al. 2014. Thromb Res. 133:244. PubMed
  364. Passaro C, et al. 2014. Mol Oncol. 9:78. PubMed
  365. Ligtenberg M, et al. 2016. J Immunol. 196: 759 - 766. PubMed
  366. Hrdinka M, et al. 2016. PLoS One. 11: 0162863. PubMed
  367. Liu H, et al. 2020. Cell Death Dis. 0.767361111. PubMed
  368. Vanoli F, et al. 2021. Oncogene. 40:5095. PubMed
  369. Liu H, et al. 2021. J Immunother Cancer. 9:. PubMed
  370. Smith CM, et al. 2021. Biochem J. 478:3331. PubMed
  371. Li Y, et al. 2021. Sci Transl Med. 13:. PubMed
  372. Meyer N, et al. 2021. J Clin Med. 10:. PubMed
  373. Zervou S, et al. 2017. PLoS One.. 10.1371/journal.pone.0182994. PubMed
  374. Ishidome T et al. 2017. EBioMedicine. 22:89-99 . PubMed
  375. Matsuda K, et al. 2021. Cell Rep Methods. 1:100122. PubMed
  376. Yoon Y, et al. 2016. Stem Cell Reports. 7:840-853. PubMed
  377. Chheda Z, et al. 2016. J Immunol. 197: 2016 - 2026. PubMed
  378. Slone E, et al. 2015. J Leukoc Biol. 98: 791 - 804. PubMed
  379. Séguier S, et al. 2013. PLoS One. 8:70937. PubMed
  380. Grujic M, et al. 2010. J Immunol. 185:1730. PubMed
  381. Wang Q, et al. 2013. Genes Dev. 27:615. PubMed
  382. Meyer N, et al. 2021. Int J Mol Sci. 22:. PubMed
  383. Kolbrink B, et al. 2022. Cell Mol Life Sci. 79:387. PubMed
  384. Pajarillo E, et al. 2020. J Biol Chem. 295:3040. PubMed
  385. Gholamrezaei M, et al. 2021. Iran J Parasitol. 15:475. PubMed
  386. Litzenburger UM, et al. 2017. Genome Biol. 18:15. PubMed
  387. Rajavel T, et al. 2017. Sci Rep. 2.665277778. PubMed
  388. Douguet L, et al. 2016. PLoS One. 11:e0166164. PubMed
  389. Toutounchian J, et al. 2016. Mol Pharmacol. 91(1):1-13. PubMed
  390. Matsumoto A, et al. 2019. J Extracell Vesicles. 9:1696517. PubMed
  391. Ishida M, et al. 2021. Int J Mol Sci. 22:. PubMed
  392. Lee DH, et al. 2020. Circ Res. 127:1182. PubMed
  393. Alsina-Sanchis E, et al. 2020. Mol Cancer Res. . PubMed
  394. Pirali T, et al. 2017. ChemMedChem. 1.570833333. PubMed
  395. L Chang, Y Li, D Kaplan 2016. Sci Rep. 6:36862. PubMed
  396. Becker A, et al. 2016. Sci Rep. 6: 33847. PubMed
  397. Kumai T, et al. 2015. Sci Rep. 5:16280. PubMed
  398. Polak R, et al. 2015. Blood. 126: 2404 - 2414. PubMed
  399. Putheti P, et al. 2010. PLoS One. 5:e8706. PubMed
  400. Molinar-Inglis O, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  401. Dhoonmoon A, et al. 2020. Nucleic Acids Res. 48:7252. PubMed
  402. Nastasi C, et al. 2021. Sci Rep. 11:1458. PubMed
  403. Hafez A, et al. 2017. Oncogenesis. 10.1038/oncsis.2017.46. PubMed
  404. Erdogan M, et al. 2022. Chem Biodivers. 19:e202200659. PubMed
  405. Galski H, et al. 2013. Biochem Pharmacol. 86:584. PubMed
  406. Tsou W, et al. 2014. J Biol Chem. 289:25750. PubMed
  407. Speth C, et al. 2013. J Infect Dis. 207:823. PubMed
  408. Gladow N, et al. 2020. PLoS One. 15:e0227734. PubMed
  409. Shadboorestan A, et al. 2022. Iran J Basic Med Sci. 25:506. PubMed
  410. Huang Y, et al. 2022. Front Cell Dev Biol. 9:837777. PubMed
  411. Babaei A, et al. 2020. Daru. 28:555. PubMed
  412. King R, et al. 2021. Sci Adv. 7:eabj5293. PubMed
  413. Cai Z, et al. 2020. Cell Rep. 31:107816. PubMed
  414. Lee HW, et al. 2018. Am J Physiol Renal Physiol. 314:F280. PubMed
  415. Ki H, et al. 2017. Biomed Pharmacother. 10.1016/j.biopha.2017.09.118. PubMed
  416. Liu B, et al. 2016. J Biol Chem. 291: 23869 - 23881. PubMed
  417. Lhuillier C, et al. 2015. J Biol Chem. 290: 16797 - 16811. PubMed
  418. Passaro C, et al. 2013. Endocr Relat Cancer. 20:633. PubMed
  419. Mulik R, et al. 2010. Int J Pharm. 398:190. PubMed
  420. Di Somma S, et al. 2019. Cancers (Basel). 1.70625. PubMed
  421. Jackson LM, et al. 2021. Nucleic Acids Res. 49:12855. PubMed
  422. Huang YL, et al. 2020. Elife. 9:00. PubMed
  423. Abu El Maaty MA, et al. 2021. Sci Adv. 7: . PubMed
  424. Bahl S, et al. 2021. Cell Death Dis. 12:469. PubMed
  425. Schuler F, et al. 2017. Nat Commun. . 10.1038/s41467-017-01850-4. PubMed
  426. Lin W, et al. 2014. PLoS One. 9:83160. PubMed
  427. Zhang X, et al. 2014. Acta Biochim Biophys Sin. 46:261. PubMed
  428. CO L, et al. 2015. Nat Commun. 6: 7739. PubMed
  429. Labi V, et al. 2019. Genes Dev. 33:1673. PubMed
  430. Fang P, et al. 2019. Commun Biol. 0.315972222. PubMed
  431. Kumar S, et al. 2019. Int J Cancer. 146:1652. PubMed
  432. Tatangelo V, et al. 2022. Front Oncol. 12:877495. PubMed
  433. Alcon C, et al. 2021. Cells. 10:. PubMed
  434. Cao H, et al. 2021. Nat Commun. 12:1792. PubMed
  435. Faria M, et al. 2017. PLoS One. 12(2):e0172689. PubMed
  436. Zhao CC, et al. 2018. Oncol Rep. 40:3392. PubMed
  437. Schleicher EM, et al. 2018. Nucleic Acids Res. 46:8908. PubMed
  438. Thomas A, et al. 2017. PLoS One.. 10.1371/journal.pone.0183542. PubMed
  439. Jones P, et al. 2016. J Biol Chem. 291: 16318 - 16327. PubMed
  440. Awojoodu A, et al. 2014. Blood. 124:1941. PubMed
  441. Intemann J, et al. 2014. Cell Signal. 26:1567. PubMed
  442. Monzen S, et al. 2022. Mol Clin Oncol. 16:19. PubMed
  443. Konda P, et al. 2022. Am J Cancer Res. 12:210. PubMed
  444. Wu J, et al. 2021. Adv Sci (Weinh). 8:e2101029. PubMed
  445. Bostanabad SY, et al. 2021. Sci Rep. 11:1539. PubMed
  446. Clements KE, et al. 2018. Nucleic Acids Res. 46:8898. PubMed
  447. Mendès C, et al. 2019. Sci Rep. 9:14107. PubMed
  448. Tu Z, et al. 2022. Cell Rep. 40:111072. PubMed
  449. Bensberg M, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  450. Paparella AS, et al. 2021. Nat Commun. 12:6285. PubMed
  451. Huang YL, et al. 2021. iScience. 24:103168. PubMed
  452. Zhang Y, et al. 2021. Cell Death Dis. 0.563194444. PubMed
  453. Kang JH, et al. 2020. Cell Death Dis. 0.803472222. PubMed
  454. Dewdney B, et al. 2020. Sci Rep. 10:16769. PubMed
  455. Simoni L, et al. 2020. Cell Rep. 33:108330. PubMed
  456. Lopes–Coelho F, et al. 2017. Oncotarget. 8:82803. PubMed
  457. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  458. Thanuthanakhun N, et al. 2017. PLoS One. . 10.1371/journal.pone.0187610. PubMed
  459. Kuntz E, et al. 2017. Nat Med. 10.1038/nm.4399. PubMed
  460. Coutaz M, et al. 2016. Sci Rep. 6:39117. PubMed
  461. Akane K, et al. 2016. Proc Natl Acad Sci U S A. 113: 2460 - 2465. PubMed
  462. Xiong R, et al. 2014. Toxicol Appl Pharmacol. 280:285. PubMed
  463. Chen Y, et al. 2013. J Exp Med. 210:2515. PubMed
  464. Lyu X, et al. 2019. BMC Genomics. 1.235416667. PubMed
  465. Jing Y, et al. 2019. J Allergy Clin Immunol. 144:1377. PubMed
  466. Quan M, et al. 2019. Oncol Rep. 41:3051. PubMed
  467. Kasikara C, et al. 2021. J Clin Invest. 131:. PubMed
  468. Ning N, et al. 2022. Theranostics. 12:5537. PubMed
  469. Bottardi S, et al. 2020. Exp Hematol. 68:88. PubMed
  470. Siddhartha V, et al. 2017. Artif Cells Nanomed Biotechnol . 10.1080/21691401.2017.1313267. PubMed
  471. He J, et al. 2017. FASEB J. 31:2893. PubMed
  472. Juel H, et al. 2013. PLoS One. 8:64619. PubMed
  473. Gill K, et al. 2013. Biochim Biophys Acta. 1830:2763. PubMed
  474. Young MM, et al. 2019. Cell Death Dis. 1.004861111. PubMed
  475. Santos I, et al. 2019. Nutrients. 2.210416667. PubMed
  476. Vancsik T, et al. 2019. Cancer Med. 3.313888889. PubMed
  477. Zheng M, et al. 2019. Proc Natl Acad Sci U S A. 116:12422. PubMed
  478. Shankman LS, et al. 2021. Curr Biol. 31:2469. PubMed
  479. Du Z, et al. 2020. J Allergy Clin Immunol. . PubMed
  480. Pahari S, et al. 2020. Autophagy. 1.375694444. PubMed
  481. Schleicher EM, et al. 2020. PLoS Genet. 16:e1009176. PubMed
  482. Zheng Q, et al. 2017. Mol Oncol. 10.1002/1878-0261.12056. PubMed
  483. Wang Y, et al. 2022. Front Cardiovasc Med. 9:876087. PubMed
  484. Langsch S, et al. 2016. Cancer Res . 76: 4160 - 4169. PubMed
  485. Xiong R, et al. 2015. Mol Pharmacol. 88: 1045 - 1054. PubMed
  486. Hellmann J, et al. 2013. J Immunol. 191:1383. PubMed
RRID
AB_2561291 (BioLegend Cat. No. 640905)
AB_2561292 (BioLegend Cat. No. 640906)
AB_2629519 (BioLegend Cat. No. 640945)

Antigen Details

Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Neuroscience
Gene ID
308 View all products for this Gene ID
UniProt
View information about Annexin V on UniProt.org

Related FAQs

How is your Annexin made and what sequence does it cover?

It is made in E. coli, covering human aa Met1-Asp320.

How does pH and staining temperature affect Annexin V-Phosphatidylserine binding?

Annexin-Phosphatidylserine binding is lost below pH 5.2 and with prolonged incubation over a temperature of 42°C.

Why do I need to use Annexin V Binding Buffer?

Annexin V binding requires the presence of calcium in the solution.  So, we provide Annexin V Binding Buffer (cat # 422201), which is optimized for the best performance of Annexin V staining.

Can I use RPMI during Annexin V staining?

It is best to follow protocol as described on the product data sheet. Moreover, RPMI 1640 has a relatively high concentration of phosphate and low calcium ion concentration, which negatively impacts Annexin binding to its target phosphatidylserine (PS). Measurement of cell death by using Annexin V may also be significantly affected by time of incubation on ice, calcium concentration, and type of medium.

Can I freeze Annexin V conjugates?

It should not be frozen as it will lead to loss of biological activity due to dimerization.

Is Annexin V suitable for conjugation with the Maxpar® kit for CyTOF®?

Maxpar® Labeling kits require the protein to be partially reduced, so the metal chelate can be introduced through an SH group in the hinge region of the reduced antibody. Human Annexin V contains only one Cysteine which was reported to be chemically inactive. Thus, the Maxpar® labeling protocol would not work with Annexin V, unless a free –SH group can be introduced to Annexin V.  For more information regarding SH-mediated conjugation of Annexin V please consult published papers such as this one.

Go To Top Version: 7    Revision Date: 10/22/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Pricing & Availability
Regulatory Status
RUO
Other Names
Annexin A5, Dead, Apoptosis
Ave. Rating
Submit a Review
Product Citations
publications
AnnexinV_FITC_092908.jpg
Human T leukemia cell line, Jurkat, non-treated (top) or treated (bottom) with BioLegend’s anti-human CD95 (EOS9.1) mAb (cat. 305704) for 6 hours, then stained with Annexin V-FITC and Propidium Iodide (PI) (cat. 421301)
  • AnnexinV_FITC_092908.jpg
    Human T leukemia cell line, Jurkat, non-treated (top) or treated (bottom) with BioLegend’s anti-human CD95 (EOS9.1) mAb (cat. 305704) for 6 hours, then stained with Annexin V-FITC and Propidium Iodide (PI) (cat. 421301)
  • B_AnnexinV_FITC_092908.jpg
See FITC spectral data
Cat # Size Price Quantity Check Availability Save
640905 25 tests DKK494
Check Availability


Need larger quantities of this item?
Request Bulk Quote
640906 100 tests DKK1153
Check Availability


Need larger quantities of this item?
Request Bulk Quote
640945 300 tests DKK2406
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry is lost and PS translocates to the external leaflet. Fluorochrome-labeled Annexin V can then be used to specifically target and identify apoptotic cells. Annexin V Binding Buffer (cat. no. 422201) is recommended for use with Annexin V staining.Annexin V binding alone cannot differentiate between apoptotic cells and necrotic. Therefore, we recommend using our Helix NP™ Blue (Cat. No. 425305), Helix NP™ Green (Cat.  No. 425303) or Helix NP™ NIR (Cat. No. 425301). Early apoptotic cells will exclude 7-AAD and PI, while late stage apoptotic cells and necrotic cells will stain positively, due to the passage of these dyes into the nucleus where they bind to DNA.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Reported Reactivity
Other Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The purified protein was conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The Annexin V solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this product is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per 100,000 - million cells in a 100 µl volume of Annexin V Binding Buffer (Cat No. 422201). It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Annexin V Staining

  1. Wash cells twice with cold BioLegend Cell Staining Buffer (Cat. No. 420201) and then resuspend cells in Annexin V Binding Buffer (Cat. No. 422201) at a concentration of 1x106 cells/mL.
  2. Transfer 100 µL of cell suspension in 5 mL test tube.
  3. Add 5 µL of fluorochrome conjugated Annexin V.
  4. Stain with a viability dye, such as PI (Cat. No. 421301), 7-AAD (Cat. Nos. 420403 & 420404), or Helix NP dyes (Cat. Nos. 425301, 425303, & 425305), if desired.
  5. Gently vortex the cells and incubate for 15 min at RT (25°C) in the dark.
  6. Add 400* µL of Annexin V Binding Buffer (Cat. No. 422201) to each tube. *For more concentrated samples, add a minimum of 200 µl of Annexin V Binding Buffer in this step.
  7. Analyze by flow cytometry.

For a better experience detecting apoptosis, we now recommend Apotracker™. Cell staining with Apotracker™ is Calcium independent. Thus, no special buffers are required, and the protocol can be shortened for single-step co-staining with other reagents.

Application References
  1. Koopman G, et al. 1994. Blood 84:1415.
  2. Vermes I, et al. 1995. J. Immunol. Methods 184:39.
  3. Dachary-Prigent J, et al. 1993. Blood 81:2554.
  4. Sekine C, et al. 2009. Int Immunol. PubMed
  5. Grujic M, et al. 2010. J. Immunol. 185:1730. PubMed
  6. Mulik RS, et al. 2010. Int J Pharm. 398:190. PubMed
  7. Gupta A, et al. 2011. Leuk Res. 35:1498. PubMed
  8. Speth C,et al. 2013. J Infect Dis. 207:823. PubMed
  9. Wang Q, et al. 2013. Genes Dev. 27:615. PubMed
  10. Gill K, et al. 2013. Biocheim Biophys Acta. 830:2763. PubMed
  11. Juel HB, et al. 2013. PLoS One. 8:64619. PubMed
  12. Hellmann J, et al. 2013. J. Immunol. 191:1383. PubMed
  13. Sequier S, et al. 2013. PLoS One. 8:70937. PubMed
  14. Galski H, et al. 2013. Biochem Pharmacol. 86:584. PubMed
  15. Chen YL, et al. 2013. J Exp Med. PubMed
  16. Lin WH, et al. 2014. PLoS One. 9:83160. PubMed
  17. Salge-Bartles U, 2014. Thromb res. 133:244. PubMed
  18. Zhang X, et al. 2014. Acta Biochim Biophys Sin. 46:261. PubMed
  19. Intermann J, et al. 2014. Cell Signal. 26:1567. PubMed
  20. de Vires M, 2014. Am J Physiol Lung Cell Mol Physiol. 307:240. PubMed
  21. M Parrula MC, et al. 2014. Virus Res. 189:206. PubMed
  22. Passaro C, et al. 2014. Mol Oncol. 9:78. PubMed
  23. Tsou WI, et al. 2014. J Biol Chem. 289:25750. PubMed
  24. Awojoodu AO, et al. 2014. Blood. 124:1941. PubMed
  25. Xiong R, et al. 2014. Toxicol Appl Pharmacol. 280:285. PubMed
  26. Liu J, et al. 2015. J Mol Cell Cardiol. 80:10. PubMed
Product Citations
  1. Uriostegui–Arcos M, et al. 2020. Open Biol. 10:200050. PubMed
  2. Zhang T, et al. 2022. EBioMedicine. 77:103872. PubMed
  3. Dhoonmoon A, et al. 2022. Nat Commun. 13:5063. PubMed
  4. Yao Z, et al. 2018. J Virol. 92:00:00. PubMed
  5. Glynn RA, et al. 2022. J Immunol. 208:371. PubMed
  6. Cao H, et al. 2022. Br J Haematol. 198:155. PubMed
  7. Ugamraj HS, et al. 2022. MAbs. 14:2095949. PubMed
  8. Cao L, et al. 2022. Cell Rep. 40:111156. PubMed
  9. Choi UY, et al. 2022. Cell Rep. 40:111234. PubMed
  10. Kimmerling RJ, et al. 2022. Commun Biol. 5:1295. PubMed
  11. Chen X, et al. 2022. Front Pharmacol. 13:1028692. PubMed
  12. Shahid AM, et al. 2022. PLoS One. 17:e0278209. PubMed
  13. Önel T, et al. 2023. Naunyn Schmiedebergs Arch Pharmacol. :. PubMed
  14. Papakonstantinou A, et al. 2022. Int J Mol Sci. 24: . PubMed
  15. Li J, et al. 2023. J Biol Chem. 299:102875. PubMed
  16. Martinelli M, et al. 2023. iScience. 26:105860. PubMed
  17. Tay HG, et al. 2023. Mol Ther. 31:825. PubMed
  18. Dean BD, et al. 2023. J Immunother Cancer. 11: . PubMed
  19. Lieschke E, et al. 2022. Cell Death Differ. 29:1004. PubMed
  20. Gallego-Murillo JS, et al. 2022. Biotechnol Bioeng. 119:3096. PubMed
  21. Du X, et al. 2023. Nucleic Acids Res. 51:1050. PubMed
  22. Vanoli F, et al. 2023. Oncogenesis. 12:8. PubMed
  23. Lei K, et al. 2023. iScience. 26:106001. PubMed
  24. Ohuchi K, et al. 2023. Biomed Rep. 18:28. PubMed
  25. Do-Thi VA, et al. 2023. Cancer Res Commun. 3:80. PubMed
  26. Perez GI, et al. 2023. Vaccines (Basel). 11:. PubMed
  27. Segaliny AI, et al. 2023. Commun Biol. 6:380. PubMed
  28. Potez M, et al. 2023. Front Oncol. 13:1124272. PubMed
  29. Woodley K, et al. 2023. Nat Commun. 14:2132. PubMed
  30. Quintela M, et al. 2023. Clin Epigenetics. 15:63. PubMed
  31. Quintela M, et al. 2023. Br J Cancer. . PubMed
  32. Zelenka T, et al. 2022. Nat Commun. 13:6954. PubMed
  33. Derudder E, et al. 2016. Proc Natl Acad Sci U S A. 113: 5065-5070. PubMed
  34. Dowling CM, et al. 2021. Sci Adv. 7:. PubMed
  35. Lin YN, et al. 2022. Oncoimmunology. 11:2027136. PubMed
  36. Kim EH, et al. 2020. Elife. 9:00. PubMed
  37. Bouhaddou M, et al. 2018. PLoS Comput Biol. 14:e1005985. PubMed
  38. Espinosa-Cueto P, et al. 2017. PLoS One.. 10.1371/journal.pone.0182126. PubMed
  39. Kitayama J, et al. 2016. PLoS One. 11: 0154542. PubMed
  40. Gedye C, et al. 2016. Sci Rep. 6: 25220. PubMed
  41. Carlsson J, et al. 2015. PLoS One. 10: 0143741. PubMed
  42. Wang Q, et al. 2022. Nat Commun. 13:3022. PubMed
  43. Alcon C, et al. 2020. Cell Death Dis. 0.898611111. PubMed
  44. Figueiredo T, et al. 2020. Mol Psychiatry. . PubMed
  45. Lopes-Coelho F, et al. 2021. Front Oncol. 11:656229. PubMed
  46. Yamauchi T et al. 2018. Cancer cell. 33(3):386-400 . PubMed
  47. Feng W, et al. 2017. Nat Commun. 0.697916667. PubMed
  48. Jones PD, et al. 2018. Arterioscler Thromb Vasc Biol. 38:1711. PubMed
  49. Olelewe C, et al. 2022. iScience. 25:104340. PubMed
  50. Gupta A, et al. 2011. Leuk Res. 35:1498. PubMed
  51. Fujii T, et al. 2015. Proc Natl Acad Sci U S A. 112: 12800 - 12805. PubMed
  52. Cardoso B, et al. 2015. PLoS One. 10: 0143897. PubMed
  53. Bowen ME, et al. 2019. Dev Cell. 50:212. PubMed
  54. Sales–Dias J, et al. 2019. PLoS One. 14:e0217002. PubMed
  55. Santos LC, et al. 2019. Nat Commun. 1.328472222. PubMed
  56. Kim Y, et al. 2020. J Neurochem. 155:177. PubMed
  57. Hassan MIA, et al. 2021. Comput Struct Biotechnol J. 19:880. PubMed
  58. Ramalingam P, et al. 2020. J Exp Med. 217:00:00. PubMed
  59. Lim JA, et al. 2018. BMC Cancer. 0.860416667. PubMed
  60. Venter C, et al. 2017. Cell Physiol Biochem. 43:1288. PubMed
  61. Fortin J, et al. 2020. Cancer Cell. 37:308. PubMed
  62. Vereertbrugghen A, et al. 2021. Front Oncol. 11:598319. PubMed
  63. Sun V, et al. 2021. Front Immunol. 12:716661. PubMed
  64. Macpherson L, et al. 2020. Nature. 577:266. PubMed
  65. Karavasili C, et al. 2017. International Journal of Pharmaceutics. 10.1016/j.ijpharm.2017.05.061. PubMed
  66. Thompson PJ et al. 2019. Cell metabolism. 29(5):1045-1060 . PubMed
  67. Noguchi F, et al. 2017. Mol Cancer Ther. 1.316666667. PubMed
  68. Kumar S, et al. 2018. Cancers (Basel). 0.724305556. PubMed
  69. Coray M, et al. 2022. Int J Mol Sci. 23:. PubMed
  70. Soprano M, et al. 2016. PLoS One. 11: 0155970. PubMed
  71. Chen MS, et al. 2019. Mol Cancer Res. 17:697. PubMed
  72. Kim OV, et al. 2022. iScience. 25:104654. PubMed
  73. Shahzadi SK, et al. 2022. Clin Sci (Lond). 136:181. PubMed
  74. Kolbrink B, et al. 2022. Biochem J. 479:259. PubMed
  75. He C, et al. 2021. Oncoimmunology. 1897295:10. PubMed
  76. Ni F, et al. 2019. Cell Stem Cell. 24:608. PubMed
  77. Ning X, et al. 2019. Mol Cell. 74:19. PubMed
  78. Greenblatt SM, et al. 2018. Cancer Cell. 33:1111. PubMed
  79. Naqvi N,et al. 2017. Sci Rep.. 10.1038/s41598-017-12753-1. PubMed
  80. Wang M, et al. 2017. Biochemical and Biophysical Research Communications. 10.1016/j.bbrc.2017.05.097. PubMed
  81. Beeravolu N, McKee C, Chaudhry G 2017. J App Toxicol. 10.1002/jat.3469. PubMed
  82. Pfalzgraff A, et al. 2016. Sci Rep. 6:31577. PubMed
  83. O'Neill K, et al. 2016. Genes Dev. 30: 973-988. PubMed
  84. Liu J, et al. 2015. J Mol Cell Cardiol. 80:10. PubMed
  85. Vries M, et al. 2014. Am J Physiol Lung Cell Mol Physiol. 307:240. PubMed
  86. Esteghamat F, et al. 2019. Nat Genet. 51:1233. PubMed
  87. Blümel E, et al. 2020. Oncoimmunology. 9:1751561. PubMed
  88. Sekine C, et al. 2009. Int Immunol. 1.079861111. PubMed
  89. Kinsella S, et al. 2021. Cell Rep. 37:109789. PubMed
  90. O'Connor MJ, et al. 2021. Oncogene. 40:4872. PubMed
  91. Parrula M, et al. 2014. Virus Res. 189:206. PubMed
  92. Brigger D, et al. 2015. Cell Death Dis. 6: e1861. PubMed
  93. Zhang J, et al. 2016. Cell Death Dis. 7:e2266. PubMed
  94. Wang D, et al. 2016. J Cell Sci. 129: 2261 - 2272. PubMed
  95. Uchtenhagen H, et al. 2016. Nat Commun. 7:12614. PubMed
  96. Geiger R, et al. 2016. Cell. 167:829-842. PubMed
  97. Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
  98. Martín Monreal MT, et al. 2021. Front Immunol. 12:716250. PubMed
  99. Schmitz RL, et al. 2021. Cells. 10:. PubMed
  100. Babaei A, et al. 2021. Adv Pharm Bull. 11:361. PubMed
  101. Madala HR, et al. 2020. Cancer Res. 3492:80. PubMed
  102. Clements KE, et al. 2020. Nat Commun. 4.706944444. PubMed
  103. Rauzan M, et al. 2017. PLoS One. 12:e0174107. PubMed
  104. Liu Y, et al. 2017. Acta Biomater. 10.1016/j.actbio.2017.08.021. PubMed
  105. Chiani M, et al. 2017. Artif Cells Nanomed Biotechnol. 10.1080/21691401.2017.1337029. PubMed
  106. Blümel E, et al. 2019. Oncoimmunology. 8:e1641387. PubMed
  107. Krauss PL, et al. 2021. Front Immunol. 12:730672. PubMed
  108. Herold N, et al. 2017. Nat Med. 23:256-263. PubMed
  109. Xie S, et al. 2021. Sci Rep. 0.660416667. PubMed
  110. Müller I, et al. 2020. Mol Cell. 970:77. PubMed
  111. Morimoto A, et al. 2021. Nat Commun. 12:2136. PubMed
  112. Norkin M, et al. 2021. Cell Rep. 35:109026. PubMed
  113. Kamiyama N, et al. 2017. Antiviral Res. 10.1016/j.antiviral.2017.08.007. PubMed
  114. Montero–Herradón S, et al. 2017. Organogenesis. 0.675. PubMed
  115. Nunes SC, et al. 2018. Sci Rep. 6.939583333. PubMed
  116. Sharif T, et al. 2018. Autophagy. 1.101388889. PubMed
  117. Eichelmann AK, et al. 2018. Sci Rep. 8:17553. PubMed
  118. Zhu Y, et al. 2022. Clin Transl Med. 12:e887. PubMed
  119. Khatib JB, et al. 2022. Oncotarget. 13:1078. PubMed
  120. Salge-Bartels U, et al. 2014. Thromb Res. 133:244. PubMed
  121. Passaro C, et al. 2014. Mol Oncol. 9:78. PubMed
  122. Ligtenberg M, et al. 2016. J Immunol. 196: 759 - 766. PubMed
  123. Hrdinka M, et al. 2016. PLoS One. 11: 0162863. PubMed
  124. Liu H, et al. 2020. Cell Death Dis. 0.767361111. PubMed
  125. Vanoli F, et al. 2021. Oncogene. 40:5095. PubMed
  126. Liu H, et al. 2021. J Immunother Cancer. 9:. PubMed
  127. Smith CM, et al. 2021. Biochem J. 478:3331. PubMed
  128. Li Y, et al. 2021. Sci Transl Med. 13:. PubMed
  129. Meyer N, et al. 2021. J Clin Med. 10:. PubMed
  130. Zervou S, et al. 2017. PLoS One.. 10.1371/journal.pone.0182994. PubMed
  131. Ishidome T et al. 2017. EBioMedicine. 22:89-99 . PubMed
  132. Matsuda K, et al. 2021. Cell Rep Methods. 1:100122. PubMed
  133. Yoon Y, et al. 2016. Stem Cell Reports. 7:840-853. PubMed
  134. Chheda Z, et al. 2016. J Immunol. 197: 2016 - 2026. PubMed
  135. Slone E, et al. 2015. J Leukoc Biol. 98: 791 - 804. PubMed
  136. Séguier S, et al. 2013. PLoS One. 8:70937. PubMed
  137. Grujic M, et al. 2010. J Immunol. 185:1730. PubMed
  138. Wang Q, et al. 2013. Genes Dev. 27:615. PubMed
  139. Meyer N, et al. 2021. Int J Mol Sci. 22:. PubMed
  140. Kolbrink B, et al. 2022. Cell Mol Life Sci. 79:387. PubMed
  141. Pajarillo E, et al. 2020. J Biol Chem. 295:3040. PubMed
  142. Gholamrezaei M, et al. 2021. Iran J Parasitol. 15:475. PubMed
  143. Litzenburger UM, et al. 2017. Genome Biol. 18:15. PubMed
  144. Rajavel T, et al. 2017. Sci Rep. 2.665277778. PubMed
  145. Douguet L, et al. 2016. PLoS One. 11:e0166164. PubMed
  146. Toutounchian J, et al. 2016. Mol Pharmacol. 91(1):1-13. PubMed
  147. Matsumoto A, et al. 2019. J Extracell Vesicles. 9:1696517. PubMed
  148. Ishida M, et al. 2021. Int J Mol Sci. 22:. PubMed
  149. Lee DH, et al. 2020. Circ Res. 127:1182. PubMed
  150. Alsina-Sanchis E, et al. 2020. Mol Cancer Res. . PubMed
  151. Pirali T, et al. 2017. ChemMedChem. 1.570833333. PubMed
  152. L Chang, Y Li, D Kaplan 2016. Sci Rep. 6:36862. PubMed
  153. Becker A, et al. 2016. Sci Rep. 6: 33847. PubMed
  154. Kumai T, et al. 2015. Sci Rep. 5:16280. PubMed
  155. Polak R, et al. 2015. Blood. 126: 2404 - 2414. PubMed
  156. Putheti P, et al. 2010. PLoS One. 5:e8706. PubMed
  157. Molinar-Inglis O, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  158. Dhoonmoon A, et al. 2020. Nucleic Acids Res. 48:7252. PubMed
  159. Nastasi C, et al. 2021. Sci Rep. 11:1458. PubMed
  160. Hafez A, et al. 2017. Oncogenesis. 10.1038/oncsis.2017.46. PubMed
  161. Erdogan M, et al. 2022. Chem Biodivers. 19:e202200659. PubMed
  162. Galski H, et al. 2013. Biochem Pharmacol. 86:584. PubMed
  163. Tsou W, et al. 2014. J Biol Chem. 289:25750. PubMed
  164. Speth C, et al. 2013. J Infect Dis. 207:823. PubMed
  165. Gladow N, et al. 2020. PLoS One. 15:e0227734. PubMed
  166. Shadboorestan A, et al. 2022. Iran J Basic Med Sci. 25:506. PubMed
  167. Huang Y, et al. 2022. Front Cell Dev Biol. 9:837777. PubMed
  168. Babaei A, et al. 2020. Daru. 28:555. PubMed
  169. King R, et al. 2021. Sci Adv. 7:eabj5293. PubMed
  170. Cai Z, et al. 2020. Cell Rep. 31:107816. PubMed
  171. Lee HW, et al. 2018. Am J Physiol Renal Physiol. 314:F280. PubMed
  172. Ki H, et al. 2017. Biomed Pharmacother. 10.1016/j.biopha.2017.09.118. PubMed
  173. Liu B, et al. 2016. J Biol Chem. 291: 23869 - 23881. PubMed
  174. Lhuillier C, et al. 2015. J Biol Chem. 290: 16797 - 16811. PubMed
  175. Passaro C, et al. 2013. Endocr Relat Cancer. 20:633. PubMed
  176. Mulik R, et al. 2010. Int J Pharm. 398:190. PubMed
  177. Di Somma S, et al. 2019. Cancers (Basel). 1.70625. PubMed
  178. Jackson LM, et al. 2021. Nucleic Acids Res. 49:12855. PubMed
  179. Huang YL, et al. 2020. Elife. 9:00. PubMed
  180. Abu El Maaty MA, et al. 2021. Sci Adv. 7: . PubMed
  181. Bahl S, et al. 2021. Cell Death Dis. 12:469. PubMed
  182. Schuler F, et al. 2017. Nat Commun. . 10.1038/s41467-017-01850-4. PubMed
  183. Lin W, et al. 2014. PLoS One. 9:83160. PubMed
  184. Zhang X, et al. 2014. Acta Biochim Biophys Sin. 46:261. PubMed
  185. CO L, et al. 2015. Nat Commun. 6: 7739. PubMed
  186. Labi V, et al. 2019. Genes Dev. 33:1673. PubMed
  187. Fang P, et al. 2019. Commun Biol. 0.315972222. PubMed
  188. Kumar S, et al. 2019. Int J Cancer. 146:1652. PubMed
  189. Tatangelo V, et al. 2022. Front Oncol. 12:877495. PubMed
  190. Alcon C, et al. 2021. Cells. 10:. PubMed
  191. Cao H, et al. 2021. Nat Commun. 12:1792. PubMed
  192. Faria M, et al. 2017. PLoS One. 12(2):e0172689. PubMed
  193. Zhao CC, et al. 2018. Oncol Rep. 40:3392. PubMed
  194. Schleicher EM, et al. 2018. Nucleic Acids Res. 46:8908. PubMed
  195. Thomas A, et al. 2017. PLoS One.. 10.1371/journal.pone.0183542. PubMed
  196. Jones P, et al. 2016. J Biol Chem. 291: 16318 - 16327. PubMed
  197. Awojoodu A, et al. 2014. Blood. 124:1941. PubMed
  198. Intemann J, et al. 2014. Cell Signal. 26:1567. PubMed
  199. Monzen S, et al. 2022. Mol Clin Oncol. 16:19. PubMed
  200. Konda P, et al. 2022. Am J Cancer Res. 12:210. PubMed
  201. Wu J, et al. 2021. Adv Sci (Weinh). 8:e2101029. PubMed
  202. Bostanabad SY, et al. 2021. Sci Rep. 11:1539. PubMed
  203. Clements KE, et al. 2018. Nucleic Acids Res. 46:8898. PubMed
  204. Mendès C, et al. 2019. Sci Rep. 9:14107. PubMed
  205. Tu Z, et al. 2022. Cell Rep. 40:111072. PubMed
  206. Bensberg M, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  207. Paparella AS, et al. 2021. Nat Commun. 12:6285. PubMed
  208. Huang YL, et al. 2021. iScience. 24:103168. PubMed
  209. Zhang Y, et al. 2021. Cell Death Dis. 0.563194444. PubMed
  210. Kang JH, et al. 2020. Cell Death Dis. 0.803472222. PubMed
  211. Dewdney B, et al. 2020. Sci Rep. 10:16769. PubMed
  212. Simoni L, et al. 2020. Cell Rep. 33:108330. PubMed
  213. Lopes–Coelho F, et al. 2017. Oncotarget. 8:82803. PubMed
  214. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  215. Thanuthanakhun N, et al. 2017. PLoS One. . 10.1371/journal.pone.0187610. PubMed
  216. Kuntz E, et al. 2017. Nat Med. 10.1038/nm.4399. PubMed
  217. Coutaz M, et al. 2016. Sci Rep. 6:39117. PubMed
  218. Akane K, et al. 2016. Proc Natl Acad Sci U S A. 113: 2460 - 2465. PubMed
  219. Xiong R, et al. 2014. Toxicol Appl Pharmacol. 280:285. PubMed
  220. Chen Y, et al. 2013. J Exp Med. 210:2515. PubMed
  221. Lyu X, et al. 2019. BMC Genomics. 1.235416667. PubMed
  222. Jing Y, et al. 2019. J Allergy Clin Immunol. 144:1377. PubMed
  223. Quan M, et al. 2019. Oncol Rep. 41:3051. PubMed
  224. Kasikara C, et al. 2021. J Clin Invest. 131:. PubMed
  225. Ning N, et al. 2022. Theranostics. 12:5537. PubMed
  226. Bottardi S, et al. 2020. Exp Hematol. 68:88. PubMed
  227. Siddhartha V, et al. 2017. Artif Cells Nanomed Biotechnol . 10.1080/21691401.2017.1313267. PubMed
  228. He J, et al. 2017. FASEB J. 31:2893. PubMed
  229. Juel H, et al. 2013. PLoS One. 8:64619. PubMed
  230. Gill K, et al. 2013. Biochim Biophys Acta. 1830:2763. PubMed
  231. Young MM, et al. 2019. Cell Death Dis. 1.004861111. PubMed
  232. Santos I, et al. 2019. Nutrients. 2.210416667. PubMed
  233. Vancsik T, et al. 2019. Cancer Med. 3.313888889. PubMed
  234. Zheng M, et al. 2019. Proc Natl Acad Sci U S A. 116:12422. PubMed
  235. Shankman LS, et al. 2021. Curr Biol. 31:2469. PubMed
  236. Du Z, et al. 2020. J Allergy Clin Immunol. . PubMed
  237. Pahari S, et al. 2020. Autophagy. 1.375694444. PubMed
  238. Schleicher EM, et al. 2020. PLoS Genet. 16:e1009176. PubMed
  239. Zheng Q, et al. 2017. Mol Oncol. 10.1002/1878-0261.12056. PubMed
  240. Wang Y, et al. 2022. Front Cardiovasc Med. 9:876087. PubMed
  241. Langsch S, et al. 2016. Cancer Res . 76: 4160 - 4169. PubMed
  242. Xiong R, et al. 2015. Mol Pharmacol. 88: 1045 - 1054. PubMed
  243. Hellmann J, et al. 2013. J Immunol. 191:1383. PubMed
  244. Uriostegui–Arcos M, et al. 2020. Open Biol. 10:200050. PubMed
  245. Zhang T, et al. 2022. EBioMedicine. 77:103872. PubMed
  246. Dhoonmoon A, et al. 2022. Nat Commun. 13:5063. PubMed
  247. Yao Z, et al. 2018. J Virol. 92:00:00. PubMed
  248. Glynn RA, et al. 2022. J Immunol. 208:371. PubMed
  249. Cao H, et al. 2022. Br J Haematol. 198:155. PubMed
  250. Ugamraj HS, et al. 2022. MAbs. 14:2095949. PubMed
  251. Cao L, et al. 2022. Cell Rep. 40:111156. PubMed
  252. Choi UY, et al. 2022. Cell Rep. 40:111234. PubMed
  253. Kimmerling RJ, et al. 2022. Commun Biol. 5:1295. PubMed
  254. Chen X, et al. 2022. Front Pharmacol. 13:1028692. PubMed
  255. Shahid AM, et al. 2022. PLoS One. 17:e0278209. PubMed
  256. Önel T, et al. 2023. Naunyn Schmiedebergs Arch Pharmacol. :. PubMed
  257. Papakonstantinou A, et al. 2022. Int J Mol Sci. 24: . PubMed
  258. Li J, et al. 2023. J Biol Chem. 299:102875. PubMed
  259. Martinelli M, et al. 2023. iScience. 26:105860. PubMed
  260. Tay HG, et al. 2023. Mol Ther. 31:825. PubMed
  261. Dean BD, et al. 2023. J Immunother Cancer. 11: . PubMed
  262. Lieschke E, et al. 2022. Cell Death Differ. 29:1004. PubMed
  263. Gallego-Murillo JS, et al. 2022. Biotechnol Bioeng. 119:3096. PubMed
  264. Du X, et al. 2023. Nucleic Acids Res. 51:1050. PubMed
  265. Vanoli F, et al. 2023. Oncogenesis. 12:8. PubMed
  266. Lei K, et al. 2023. iScience. 26:106001. PubMed
  267. Ohuchi K, et al. 2023. Biomed Rep. 18:28. PubMed
  268. Do-Thi VA, et al. 2023. Cancer Res Commun. 3:80. PubMed
  269. Perez GI, et al. 2023. Vaccines (Basel). 11:. PubMed
  270. Segaliny AI, et al. 2023. Commun Biol. 6:380. PubMed
  271. Potez M, et al. 2023. Front Oncol. 13:1124272. PubMed
  272. Woodley K, et al. 2023. Nat Commun. 14:2132. PubMed
  273. Quintela M, et al. 2023. Clin Epigenetics. 15:63. PubMed
  274. Quintela M, et al. 2023. Br J Cancer. . PubMed
  275. Zelenka T, et al. 2022. Nat Commun. 13:6954. PubMed
  276. Derudder E, et al. 2016. Proc Natl Acad Sci U S A. 113: 5065-5070. PubMed
  277. Dowling CM, et al. 2021. Sci Adv. 7:. PubMed
  278. Lin YN, et al. 2022. Oncoimmunology. 11:2027136. PubMed
  279. Kim EH, et al. 2020. Elife. 9:00. PubMed
  280. Bouhaddou M, et al. 2018. PLoS Comput Biol. 14:e1005985. PubMed
  281. Espinosa-Cueto P, et al. 2017. PLoS One.. 10.1371/journal.pone.0182126. PubMed
  282. Kitayama J, et al. 2016. PLoS One. 11: 0154542. PubMed
  283. Gedye C, et al. 2016. Sci Rep. 6: 25220. PubMed
  284. Carlsson J, et al. 2015. PLoS One. 10: 0143741. PubMed
  285. Wang Q, et al. 2022. Nat Commun. 13:3022. PubMed
  286. Alcon C, et al. 2020. Cell Death Dis. 0.898611111. PubMed
  287. Figueiredo T, et al. 2020. Mol Psychiatry. . PubMed
  288. Lopes-Coelho F, et al. 2021. Front Oncol. 11:656229. PubMed
  289. Yamauchi T et al. 2018. Cancer cell. 33(3):386-400 . PubMed
  290. Feng W, et al. 2017. Nat Commun. 0.697916667. PubMed
  291. Jones PD, et al. 2018. Arterioscler Thromb Vasc Biol. 38:1711. PubMed
  292. Olelewe C, et al. 2022. iScience. 25:104340. PubMed
  293. Gupta A, et al. 2011. Leuk Res. 35:1498. PubMed
  294. Fujii T, et al. 2015. Proc Natl Acad Sci U S A. 112: 12800 - 12805. PubMed
  295. Cardoso B, et al. 2015. PLoS One. 10: 0143897. PubMed
  296. Bowen ME, et al. 2019. Dev Cell. 50:212. PubMed
  297. Sales–Dias J, et al. 2019. PLoS One. 14:e0217002. PubMed
  298. Santos LC, et al. 2019. Nat Commun. 1.328472222. PubMed
  299. Kim Y, et al. 2020. J Neurochem. 155:177. PubMed
  300. Hassan MIA, et al. 2021. Comput Struct Biotechnol J. 19:880. PubMed
  301. Ramalingam P, et al. 2020. J Exp Med. 217:00:00. PubMed
  302. Lim JA, et al. 2018. BMC Cancer. 0.860416667. PubMed
  303. Venter C, et al. 2017. Cell Physiol Biochem. 43:1288. PubMed
  304. Fortin J, et al. 2020. Cancer Cell. 37:308. PubMed
  305. Vereertbrugghen A, et al. 2021. Front Oncol. 11:598319. PubMed
  306. Sun V, et al. 2021. Front Immunol. 12:716661. PubMed
  307. Macpherson L, et al. 2020. Nature. 577:266. PubMed
  308. Karavasili C, et al. 2017. International Journal of Pharmaceutics. 10.1016/j.ijpharm.2017.05.061. PubMed
  309. Thompson PJ et al. 2019. Cell metabolism. 29(5):1045-1060 . PubMed
  310. Noguchi F, et al. 2017. Mol Cancer Ther. 1.316666667. PubMed
  311. Kumar S, et al. 2018. Cancers (Basel). 0.724305556. PubMed
  312. Coray M, et al. 2022. Int J Mol Sci. 23:. PubMed
  313. Soprano M, et al. 2016. PLoS One. 11: 0155970. PubMed
  314. Chen MS, et al. 2019. Mol Cancer Res. 17:697. PubMed
  315. Kim OV, et al. 2022. iScience. 25:104654. PubMed
  316. Shahzadi SK, et al. 2022. Clin Sci (Lond). 136:181. PubMed
  317. Kolbrink B, et al. 2022. Biochem J. 479:259. PubMed
  318. He C, et al. 2021. Oncoimmunology. 1897295:10. PubMed
  319. Ni F, et al. 2019. Cell Stem Cell. 24:608. PubMed
  320. Ning X, et al. 2019. Mol Cell. 74:19. PubMed
  321. Greenblatt SM, et al. 2018. Cancer Cell. 33:1111. PubMed
  322. Naqvi N,et al. 2017. Sci Rep.. 10.1038/s41598-017-12753-1. PubMed
  323. Wang M, et al. 2017. Biochemical and Biophysical Research Communications. 10.1016/j.bbrc.2017.05.097. PubMed
  324. Beeravolu N, McKee C, Chaudhry G 2017. J App Toxicol. 10.1002/jat.3469. PubMed
  325. Pfalzgraff A, et al. 2016. Sci Rep. 6:31577. PubMed
  326. O'Neill K, et al. 2016. Genes Dev. 30: 973-988. PubMed
  327. Liu J, et al. 2015. J Mol Cell Cardiol. 80:10. PubMed
  328. Vries M, et al. 2014. Am J Physiol Lung Cell Mol Physiol. 307:240. PubMed
  329. Esteghamat F, et al. 2019. Nat Genet. 51:1233. PubMed
  330. Blümel E, et al. 2020. Oncoimmunology. 9:1751561. PubMed
  331. Sekine C, et al. 2009. Int Immunol. 1.079861111. PubMed
  332. Kinsella S, et al. 2021. Cell Rep. 37:109789. PubMed
  333. O'Connor MJ, et al. 2021. Oncogene. 40:4872. PubMed
  334. Parrula M, et al. 2014. Virus Res. 189:206. PubMed
  335. Brigger D, et al. 2015. Cell Death Dis. 6: e1861. PubMed
  336. Zhang J, et al. 2016. Cell Death Dis. 7:e2266. PubMed
  337. Wang D, et al. 2016. J Cell Sci. 129: 2261 - 2272. PubMed
  338. Uchtenhagen H, et al. 2016. Nat Commun. 7:12614. PubMed
  339. Geiger R, et al. 2016. Cell. 167:829-842. PubMed
  340. Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
  341. Martín Monreal MT, et al. 2021. Front Immunol. 12:716250. PubMed
  342. Schmitz RL, et al. 2021. Cells. 10:. PubMed
  343. Babaei A, et al. 2021. Adv Pharm Bull. 11:361. PubMed
  344. Madala HR, et al. 2020. Cancer Res. 3492:80. PubMed
  345. Clements KE, et al. 2020. Nat Commun. 4.706944444. PubMed
  346. Rauzan M, et al. 2017. PLoS One. 12:e0174107. PubMed
  347. Liu Y, et al. 2017. Acta Biomater. 10.1016/j.actbio.2017.08.021. PubMed
  348. Chiani M, et al. 2017. Artif Cells Nanomed Biotechnol. 10.1080/21691401.2017.1337029. PubMed
  349. Blümel E, et al. 2019. Oncoimmunology. 8:e1641387. PubMed
  350. Krauss PL, et al. 2021. Front Immunol. 12:730672. PubMed
  351. Herold N, et al. 2017. Nat Med. 23:256-263. PubMed
  352. Xie S, et al. 2021. Sci Rep. 0.660416667. PubMed
  353. Müller I, et al. 2020. Mol Cell. 970:77. PubMed
  354. Morimoto A, et al. 2021. Nat Commun. 12:2136. PubMed
  355. Norkin M, et al. 2021. Cell Rep. 35:109026. PubMed
  356. Kamiyama N, et al. 2017. Antiviral Res. 10.1016/j.antiviral.2017.08.007. PubMed
  357. Montero–Herradón S, et al. 2017. Organogenesis. 0.675. PubMed
  358. Nunes SC, et al. 2018. Sci Rep. 6.939583333. PubMed
  359. Sharif T, et al. 2018. Autophagy. 1.101388889. PubMed
  360. Eichelmann AK, et al. 2018. Sci Rep. 8:17553. PubMed
  361. Zhu Y, et al. 2022. Clin Transl Med. 12:e887. PubMed
  362. Khatib JB, et al. 2022. Oncotarget. 13:1078. PubMed
  363. Salge-Bartels U, et al. 2014. Thromb Res. 133:244. PubMed
  364. Passaro C, et al. 2014. Mol Oncol. 9:78. PubMed
  365. Ligtenberg M, et al. 2016. J Immunol. 196: 759 - 766. PubMed
  366. Hrdinka M, et al. 2016. PLoS One. 11: 0162863. PubMed
  367. Liu H, et al. 2020. Cell Death Dis. 0.767361111. PubMed
  368. Vanoli F, et al. 2021. Oncogene. 40:5095. PubMed
  369. Liu H, et al. 2021. J Immunother Cancer. 9:. PubMed
  370. Smith CM, et al. 2021. Biochem J. 478:3331. PubMed
  371. Li Y, et al. 2021. Sci Transl Med. 13:. PubMed
  372. Meyer N, et al. 2021. J Clin Med. 10:. PubMed
  373. Zervou S, et al. 2017. PLoS One.. 10.1371/journal.pone.0182994. PubMed
  374. Ishidome T et al. 2017. EBioMedicine. 22:89-99 . PubMed
  375. Matsuda K, et al. 2021. Cell Rep Methods. 1:100122. PubMed
  376. Yoon Y, et al. 2016. Stem Cell Reports. 7:840-853. PubMed
  377. Chheda Z, et al. 2016. J Immunol. 197: 2016 - 2026. PubMed
  378. Slone E, et al. 2015. J Leukoc Biol. 98: 791 - 804. PubMed
  379. Séguier S, et al. 2013. PLoS One. 8:70937. PubMed
  380. Grujic M, et al. 2010. J Immunol. 185:1730. PubMed
  381. Wang Q, et al. 2013. Genes Dev. 27:615. PubMed
  382. Meyer N, et al. 2021. Int J Mol Sci. 22:. PubMed
  383. Kolbrink B, et al. 2022. Cell Mol Life Sci. 79:387. PubMed
  384. Pajarillo E, et al. 2020. J Biol Chem. 295:3040. PubMed
  385. Gholamrezaei M, et al. 2021. Iran J Parasitol. 15:475. PubMed
  386. Litzenburger UM, et al. 2017. Genome Biol. 18:15. PubMed
  387. Rajavel T, et al. 2017. Sci Rep. 2.665277778. PubMed
  388. Douguet L, et al. 2016. PLoS One. 11:e0166164. PubMed
  389. Toutounchian J, et al. 2016. Mol Pharmacol. 91(1):1-13. PubMed
  390. Matsumoto A, et al. 2019. J Extracell Vesicles. 9:1696517. PubMed
  391. Ishida M, et al. 2021. Int J Mol Sci. 22:. PubMed
  392. Lee DH, et al. 2020. Circ Res. 127:1182. PubMed
  393. Alsina-Sanchis E, et al. 2020. Mol Cancer Res. . PubMed
  394. Pirali T, et al. 2017. ChemMedChem. 1.570833333. PubMed
  395. L Chang, Y Li, D Kaplan 2016. Sci Rep. 6:36862. PubMed
  396. Becker A, et al. 2016. Sci Rep. 6: 33847. PubMed
  397. Kumai T, et al. 2015. Sci Rep. 5:16280. PubMed
  398. Polak R, et al. 2015. Blood. 126: 2404 - 2414. PubMed
  399. Putheti P, et al. 2010. PLoS One. 5:e8706. PubMed
  400. Molinar-Inglis O, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  401. Dhoonmoon A, et al. 2020. Nucleic Acids Res. 48:7252. PubMed
  402. Nastasi C, et al. 2021. Sci Rep. 11:1458. PubMed
  403. Hafez A, et al. 2017. Oncogenesis. 10.1038/oncsis.2017.46. PubMed
  404. Erdogan M, et al. 2022. Chem Biodivers. 19:e202200659. PubMed
  405. Galski H, et al. 2013. Biochem Pharmacol. 86:584. PubMed
  406. Tsou W, et al. 2014. J Biol Chem. 289:25750. PubMed
  407. Speth C, et al. 2013. J Infect Dis. 207:823. PubMed
  408. Gladow N, et al. 2020. PLoS One. 15:e0227734. PubMed
  409. Shadboorestan A, et al. 2022. Iran J Basic Med Sci. 25:506. PubMed
  410. Huang Y, et al. 2022. Front Cell Dev Biol. 9:837777. PubMed
  411. Babaei A, et al. 2020. Daru. 28:555. PubMed
  412. King R, et al. 2021. Sci Adv. 7:eabj5293. PubMed
  413. Cai Z, et al. 2020. Cell Rep. 31:107816. PubMed
  414. Lee HW, et al. 2018. Am J Physiol Renal Physiol. 314:F280. PubMed
  415. Ki H, et al. 2017. Biomed Pharmacother. 10.1016/j.biopha.2017.09.118. PubMed
  416. Liu B, et al. 2016. J Biol Chem. 291: 23869 - 23881. PubMed
  417. Lhuillier C, et al. 2015. J Biol Chem. 290: 16797 - 16811. PubMed
  418. Passaro C, et al. 2013. Endocr Relat Cancer. 20:633. PubMed
  419. Mulik R, et al. 2010. Int J Pharm. 398:190. PubMed
  420. Di Somma S, et al. 2019. Cancers (Basel). 1.70625. PubMed
  421. Jackson LM, et al. 2021. Nucleic Acids Res. 49:12855. PubMed
  422. Huang YL, et al. 2020. Elife. 9:00. PubMed
  423. Abu El Maaty MA, et al. 2021. Sci Adv. 7: . PubMed
  424. Bahl S, et al. 2021. Cell Death Dis. 12:469. PubMed
  425. Schuler F, et al. 2017. Nat Commun. . 10.1038/s41467-017-01850-4. PubMed
  426. Lin W, et al. 2014. PLoS One. 9:83160. PubMed
  427. Zhang X, et al. 2014. Acta Biochim Biophys Sin. 46:261. PubMed
  428. CO L, et al. 2015. Nat Commun. 6: 7739. PubMed
  429. Labi V, et al. 2019. Genes Dev. 33:1673. PubMed
  430. Fang P, et al. 2019. Commun Biol. 0.315972222. PubMed
  431. Kumar S, et al. 2019. Int J Cancer. 146:1652. PubMed
  432. Tatangelo V, et al. 2022. Front Oncol. 12:877495. PubMed
  433. Alcon C, et al. 2021. Cells. 10:. PubMed
  434. Cao H, et al. 2021. Nat Commun. 12:1792. PubMed
  435. Faria M, et al. 2017. PLoS One. 12(2):e0172689. PubMed
  436. Zhao CC, et al. 2018. Oncol Rep. 40:3392. PubMed
  437. Schleicher EM, et al. 2018. Nucleic Acids Res. 46:8908. PubMed
  438. Thomas A, et al. 2017. PLoS One.. 10.1371/journal.pone.0183542. PubMed
  439. Jones P, et al. 2016. J Biol Chem. 291: 16318 - 16327. PubMed
  440. Awojoodu A, et al. 2014. Blood. 124:1941. PubMed
  441. Intemann J, et al. 2014. Cell Signal. 26:1567. PubMed
  442. Monzen S, et al. 2022. Mol Clin Oncol. 16:19. PubMed
  443. Konda P, et al. 2022. Am J Cancer Res. 12:210. PubMed
  444. Wu J, et al. 2021. Adv Sci (Weinh). 8:e2101029. PubMed
  445. Bostanabad SY, et al. 2021. Sci Rep. 11:1539. PubMed
  446. Clements KE, et al. 2018. Nucleic Acids Res. 46:8898. PubMed
  447. Mendès C, et al. 2019. Sci Rep. 9:14107. PubMed
  448. Tu Z, et al. 2022. Cell Rep. 40:111072. PubMed
  449. Bensberg M, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  450. Paparella AS, et al. 2021. Nat Commun. 12:6285. PubMed
  451. Huang YL, et al. 2021. iScience. 24:103168. PubMed
  452. Zhang Y, et al. 2021. Cell Death Dis. 0.563194444. PubMed
  453. Kang JH, et al. 2020. Cell Death Dis. 0.803472222. PubMed
  454. Dewdney B, et al. 2020. Sci Rep. 10:16769. PubMed
  455. Simoni L, et al. 2020. Cell Rep. 33:108330. PubMed
  456. Lopes–Coelho F, et al. 2017. Oncotarget. 8:82803. PubMed
  457. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  458. Thanuthanakhun N, et al. 2017. PLoS One. . 10.1371/journal.pone.0187610. PubMed
  459. Kuntz E, et al. 2017. Nat Med. 10.1038/nm.4399. PubMed
  460. Coutaz M, et al. 2016. Sci Rep. 6:39117. PubMed
  461. Akane K, et al. 2016. Proc Natl Acad Sci U S A. 113: 2460 - 2465. PubMed
  462. Xiong R, et al. 2014. Toxicol Appl Pharmacol. 280:285. PubMed
  463. Chen Y, et al. 2013. J Exp Med. 210:2515. PubMed
  464. Lyu X, et al. 2019. BMC Genomics. 1.235416667. PubMed
  465. Jing Y, et al. 2019. J Allergy Clin Immunol. 144:1377. PubMed
  466. Quan M, et al. 2019. Oncol Rep. 41:3051. PubMed
  467. Kasikara C, et al. 2021. J Clin Invest. 131:. PubMed
  468. Ning N, et al. 2022. Theranostics. 12:5537. PubMed
  469. Bottardi S, et al. 2020. Exp Hematol. 68:88. PubMed
  470. Siddhartha V, et al. 2017. Artif Cells Nanomed Biotechnol . 10.1080/21691401.2017.1313267. PubMed
  471. He J, et al. 2017. FASEB J. 31:2893. PubMed
  472. Juel H, et al. 2013. PLoS One. 8:64619. PubMed
  473. Gill K, et al. 2013. Biochim Biophys Acta. 1830:2763. PubMed
  474. Young MM, et al. 2019. Cell Death Dis. 1.004861111. PubMed
  475. Santos I, et al. 2019. Nutrients. 2.210416667. PubMed
  476. Vancsik T, et al. 2019. Cancer Med. 3.313888889. PubMed
  477. Zheng M, et al. 2019. Proc Natl Acad Sci U S A. 116:12422. PubMed
  478. Shankman LS, et al. 2021. Curr Biol. 31:2469. PubMed
  479. Du Z, et al. 2020. J Allergy Clin Immunol. . PubMed
  480. Pahari S, et al. 2020. Autophagy. 1.375694444. PubMed
  481. Schleicher EM, et al. 2020. PLoS Genet. 16:e1009176. PubMed
  482. Zheng Q, et al. 2017. Mol Oncol. 10.1002/1878-0261.12056. PubMed
  483. Wang Y, et al. 2022. Front Cardiovasc Med. 9:876087. PubMed
  484. Langsch S, et al. 2016. Cancer Res . 76: 4160 - 4169. PubMed
  485. Xiong R, et al. 2015. Mol Pharmacol. 88: 1045 - 1054. PubMed
  486. Hellmann J, et al. 2013. J Immunol. 191:1383. PubMed
RRID
AB_2561291 (BioLegend Cat. No. 640905)
AB_2561292 (BioLegend Cat. No. 640906)
AB_2629519 (BioLegend Cat. No. 640945)

Antigen Details

Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Neuroscience
Gene ID
308 View all products for this Gene ID
UniProt
View information about Annexin V on UniProt.org

Related FAQs

How is your Annexin made and what sequence does it cover?

It is made in E. coli, covering human aa Met1-Asp320.

How does pH and staining temperature affect Annexin V-Phosphatidylserine binding?

Annexin-Phosphatidylserine binding is lost below pH 5.2 and with prolonged incubation over a temperature of 42°C.

Why do I need to use Annexin V Binding Buffer?

Annexin V binding requires the presence of calcium in the solution.  So, we provide Annexin V Binding Buffer (cat # 422201), which is optimized for the best performance of Annexin V staining.

Can I use RPMI during Annexin V staining?

It is best to follow protocol as described on the product data sheet. Moreover, RPMI 1640 has a relatively high concentration of phosphate and low calcium ion concentration, which negatively impacts Annexin binding to its target phosphatidylserine (PS). Measurement of cell death by using Annexin V may also be significantly affected by time of incubation on ice, calcium concentration, and type of medium.

Can I freeze Annexin V conjugates?

It should not be frozen as it will lead to loss of biological activity due to dimerization.

Is Annexin V suitable for conjugation with the Maxpar® kit for CyTOF®?

Maxpar® Labeling kits require the protein to be partially reduced, so the metal chelate can be introduced through an SH group in the hinge region of the reduced antibody. Human Annexin V contains only one Cysteine which was reported to be chemically inactive. Thus, the Maxpar® labeling protocol would not work with Annexin V, unless a free –SH group can be introduced to Annexin V.  For more information regarding SH-mediated conjugation of Annexin V please consult published papers such as this one.

Go To Top Version: 7    Revision Date: 10/22/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account