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FITC anti-human CD3 Antibody FITC anti-human CD3 Antibody

Pricing & Availability
Clone
UCHT1 (See other available formats)
Regulatory Status
RUO
Workshop
III 471
Other Names
T3, CD3ε
Isotype
Mouse IgG1, κ
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Product Citations
publications
UCHT1_FITC_021805
Human peripheral blood lymphocytes stained with UCHT1 FITC
  • UCHT1_FITC_021805
    Human peripheral blood lymphocytes stained with UCHT1 FITC
Compare all formats See FITC spectral data
Cat # Size Price Quantity Check Availability Save
300405 25 tests DKK131
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300406 100 tests DKK297
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300452 100 µg DKK321
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300440 500 tests DKK1212
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Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NKT cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
µg sizes: 0.5 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, Western blotting9, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Application References
  1. Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP)
  2. Graves J, et al. 1991. J. Immunol. 146:2102. (Activ)
  3. Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ)
  4. Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC)
  5. Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ)
  6. Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC)
  7. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  8. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  9. Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
  10. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  11. Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC)
  12. Luckashenak N, et al. 2013. J. Immunol. 190:27. PubMed
  13. Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed
  14. Li J, et al. 2015. Cancer Res. 75:508. PubMed
  15. Stoeckius M, et al. 2017. Nat. Methods. 14:865-868. (PG)
  16. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  17. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Johnson DC, et al. 2018. Nat Med. 24:1151. PubMed
  2. Sajadi MM, et al. 2018. Cell. 173:1783. PubMed
  3. Qin L, et al. 2022. Sci Adv. 8:eabn3774. PubMed
  4. Gan X, et al. 2022. Proc Natl Acad Sci U S A. 119:e2200879119. PubMed
  5. Sušac L, et al. 2022. Cell. 185:3201. PubMed
  6. Saber MM, et al. 2022. J Immunol Res. 2022:7219207. PubMed
  7. McEvoy CM, et al. 2022. Nat Commun. 13:7634. PubMed
  8. Graciliano NG, et al. 2023. Front Immunol. 13:1031248. PubMed
  9. Sun W, et al. 2023. Nature. 614:309. PubMed
  10. Namvar N, et al. 2022. Iran J Allergy Asthma Immunol. 21:600. PubMed
  11. Zhong J, et al. 2023. Front Immunol. 13:1081889. PubMed
  12. Yen M, et al. 2022. Cell. 185:1414. PubMed
  13. Shang P, et al. 2022. Acta Biochim Biophys Sin (Shanghai). 54:1497. PubMed
  14. Porbahaie M, et al. 2023. PLoS One. 18:e0279626. PubMed
  15. Li H, et al. 2023. Clin Rheumatol. 42:1327. PubMed
  16. Freund EC, et al. 2023. Methods Mol Biol. 2618:201. PubMed
  17. Gao F, et al. 2023. Immunity. 56:864. PubMed
  18. Lütge M, et al. 2023. Nat Immunol. . PubMed
  19. Imai H, et al. 2023. iScience. 26:106822. PubMed
  20. Patskovsky Y, et al. 2023. Nat Commun. 14:3763. PubMed
  21. Cao B, et al. 2022. Nat Commun. 13:6203. PubMed
  22. Soday L, et al. 2021. Frontiers in Immunology. 12:600056. PubMed
  23. Lin L, et al. 2020. NAR Genom Bioinform. 2:lqaa059. PubMed
  24. Carre C, et al. 2021. iScience. 24:102970. PubMed
  25. Jiang G, et al. 2020. Aging (Albany NY). 12:11466. PubMed
  26. Bhattacharya S, et al. 2020. Front Bioeng Biotechnol. 1.055555556. PubMed
  27. Barrow AD et al. 2018. Cell. 172(3):534-548 . PubMed
  28. Ye Y, et al. 2022. Nat Commun. 13:6458. PubMed
  29. Li C, et al. 2022. J Exp Med. 219:. PubMed
  30. Netsrithong R, et al. 2020. Stem Cell Research. 39:101487.. PubMed
  31. Rendeiro AF, et al. 2020. Nat Commun. 11:577. PubMed
  32. Ryzhov S, et al. 2019. J Am Heart Assoc. 8:e010874. PubMed
  33. Klemm F, et al. 2020. Cell. 181(7):1643-1660.e17. PubMed
  34. Chen L, et al. 2018. Immunology. 155:123. PubMed
  35. Kimura I, et al. 2022. Cell Rep. 38:110218. PubMed
  36. Chen M, et al. 2021. Cancers (Basel). 13:. PubMed
  37. Laustsen A, et al. 2021. Elife. 10:. PubMed
  38. Pholcharee T, et al. 2021. Nat Commun. 12:1063. PubMed
  39. Rolandelli A, et al. 2017. Sci Rep. 7:40666. PubMed
  40. Karlsson H, et al. 2015. PLoS One. 10: 0144787. PubMed
  41. Eri Yamada et al. 2018. Cell host & microbe. 23(1):110-120 . PubMed
  42. Ruetsche D, et al. 2022. Cells. 11:. PubMed
  43. Esaulova E, et al. 2020. Cell Host Microbe. 29(2):165-178.e8. PubMed
  44. Liu C, et al. 2021. Cell Res. 31:1106. PubMed
  45. Devalraju KP, et al. 2021. PLoS One. 16:e0257185. PubMed
  46. Engelmann C, et al. 2017. J Transl Med. 10.1186/s12967-017-1288-3. PubMed
  47. Zhan Y, et al. 2021. JCI Insight. 6:. PubMed
  48. Perelman SS, et al. 2021. Nat Microbiol. 6:731. PubMed
  49. Zirbes A, et al. 2021. J Mammary Gland Biol Neoplasia. 26:247. PubMed
  50. Jiang Y, et al. 2020. Clin Immunol. 218:108516. PubMed
  51. Bergamaschi L, et al. 2021. Immunity. 54(6):1257-1275.e8. PubMed
  52. Maritz MF, et al. 2018. PLoS One. 13:e0196043. PubMed
  53. Appios A, et al. 2021. Bio Protoc. 11:e4044. PubMed
  54. Yarmarkovich M, et al. 2021. Nature. 599:477. PubMed
  55. Shevyrev D, et al. 2021. Exp Ther Med. 209:21. PubMed
  56. Caduff N, et al. 2021. Cell Reports. 35(5):109056. PubMed
  57. Ping Y, et al. 2020. Front Cell Dev Biol. 0.890972222. PubMed
  58. Yuan Z, et al. 2018. Emerg Microbes Infect. 7:59. PubMed
  59. Ramos RN, et al. 2022. Clin Transl Immunology. 11:e1392. PubMed
  60. Tu HA, et al. 2020. Cell Rep Med. 1:100155. PubMed
  61. Corleis B, et al. 2017. PLoS One. 12:e0173161. PubMed
  62. Ray RM, et al. 2019. Sci Rep. 9:4334. PubMed
  63. Rajamanickam V, et al. 2021. Cancer Immunol Res. 9:602. PubMed
  64. Laroni A, et al. 2016. J Autoimmun. 72:8-18. PubMed
  65. Sawasdee N, et al. 2022. Int J Mol Med. 49:. PubMed
  66. Mishra HK, et al. 2021. Front Immunol. 12:711621. PubMed
  67. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  68. Minervina AA, et al. 2022. Nat Immunol. 23:781. PubMed
  69. Cai D, et al. 2020. Cell Mol Immunol. 17:227. PubMed
  70. Krishnan S, et al. 2021. Clin Exp Immunol. 203:458. PubMed
  71. Rossignol ED, et al. 2021. Cell Reports. 35(8):109167. PubMed
  72. Huang N, et al. 2020. Genome Biol. 1.03125. PubMed
  73. Coufal S, et al. 2019. Cells. 8. PubMed
  74. Jin S, et al. 2020. Cell Res. 30:950. PubMed
  75. Liu Y, et al. 2020. Methods Mol Biol. 2111:285. PubMed
  76. Whitfield SJC, et al. 2017. J Immunol. 198:3989. PubMed
  77. Cizmeci D, et al. 2021. eLife. 10:00. PubMed
  78. Platten M, et al. 2021. Nature. 592:463. PubMed
  79. Kariminia A, et al. 2016. Blood. 127: 3082 - 3091. PubMed
  80. Lima J, et al. 2016. Reprod Sci. 10.1177/1933719116653680. PubMed
  81. Meermeier E, et al. 2016. Nat Commun. 7:12506. PubMed
  82. Wang J, et al. 2019. Oncol Lett. 4.948611111. PubMed
  83. Kato M, et al. 2021. PLoS One. 16:e0252116. PubMed
  84. Bak S, et al. 2019. Front Immunol. 9:2953. PubMed
  85. Setliff I, et al. 2018. Cell Host Microbe. 23:845. PubMed
  86. Collins PL et al. 2018. Cell. 176(1-2):348-360 . PubMed
  87. Cecotto L, et al. 2022. iScience. 25:105211. PubMed
  88. Shin CH, et al. 2020. J Immunol. 205:3179. PubMed
  89. Hakki S, et al. 2022. Sci Rep. 12:1427. PubMed
  90. Chapelin F, et al. 2017. Sci Rep.. 10.1038/s41598-017-17669-4. PubMed
  91. Reuschl AK, et al. 2022. Cell Rep. 39:110650. PubMed
  92. Abe Y, et al. 2022. Nat Cell Biol. 24:565. PubMed
  93. Cipriano M, et al. 2022. Commun Biol. 5:52. PubMed
  94. Kaito Y, et al. 2022. Oncol Lett. 23:51. PubMed
  95. Wang Y, et al. 2021. Sci Adv. 7: . PubMed
  96. Cantoni C, et al. 2021. Oncoimmunology. 1879530:10. PubMed
  97. Motozono C, et al. 2021. Cell Host Microbe. . PubMed
  98. Capone S, et al. 2020. NPJ Vaccines. 5:94. PubMed
  99. Domanska D, et al. 2022. J Exp Med. 219:. PubMed
  100. van Erp EA, et al. 2020. Clin Transl Immunology. 9:e1112. PubMed
  101. Maas RR, et al. 2021. Nat Protoc. 16:4692. PubMed
  102. Duhen R, et al. 2021. Nat Commun. 12:1047. PubMed
  103. Wang Z, et al. 2021. Nat Commun. 12:1724. PubMed
  104. Nakatsugawa M, et al. 2015. J Immunol. 194 3487 . PubMed
  105. Nakatsugawa M, et al. 2016. Sci Rep. 6:23821. PubMed
  106. Tamai Y, et al. 2013. J Immunol. 190:4382. PubMed
  107. Beyer AI, et al. 2017. Stem Cells Dev. 26:102. PubMed
  108. Lee M, et al. 2022. Front Cell Dev Biol. 10:909557. PubMed
  109. Albrecht M, et al. 2022. Clin Transl Immunology. 11:e1398. PubMed
  110. Rambaldi B, et al. 2022. Haematologica. 107:2617. PubMed
  111. Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed
  112. Chen YP, et al. 2020. Cell Res. 30:1024. PubMed
  113. Dyson MR, et al. 2020. MAbs. 12:1829335. PubMed
  114. Ochi T, et al. 2015. Cancer Immunol Res. 3: 1070-1081. PubMed
  115. Suzuki D, et al. 2020. Stem Cell Reports. 14:49. PubMed
  116. Wang SQ, et al. 2021. J Viral Hepat. 28:795. PubMed
  117. Pappa V, et al. 2021. J Neuroimmunol. 353:577524. PubMed
  118. Vanoni G, et al. 2021. eLife. 10:00. PubMed
  119. Goc J, et al. 2021. Cell. 184:5015. PubMed
  120. Wang S, et al. 2022. Cancer Immunol Immunother. Online ahead of print. PubMed
  121. Schmidts A, et al. 2020. J Immunother Cancer. 8:. PubMed
  122. Bennstein SB, et al. 2020. eLife. 9:e55232.. PubMed
  123. Chang ZL, et al. 2020. Nature Protocols. 15(4):1507-1524.. PubMed
  124. Johnson DC, et al. 2018. Nat Med. 24:1151. PubMed
  125. Sajadi MM, et al. 2018. Cell. 173:1783. PubMed
  126. Qin L, et al. 2022. Sci Adv. 8:eabn3774. PubMed
  127. Gan X, et al. 2022. Proc Natl Acad Sci U S A. 119:e2200879119. PubMed
  128. Sušac L, et al. 2022. Cell. 185:3201. PubMed
  129. Saber MM, et al. 2022. J Immunol Res. 2022:7219207. PubMed
  130. McEvoy CM, et al. 2022. Nat Commun. 13:7634. PubMed
  131. Graciliano NG, et al. 2023. Front Immunol. 13:1031248. PubMed
  132. Sun W, et al. 2023. Nature. 614:309. PubMed
  133. Namvar N, et al. 2022. Iran J Allergy Asthma Immunol. 21:600. PubMed
  134. Zhong J, et al. 2023. Front Immunol. 13:1081889. PubMed
  135. Yen M, et al. 2022. Cell. 185:1414. PubMed
  136. Shang P, et al. 2022. Acta Biochim Biophys Sin (Shanghai). 54:1497. PubMed
  137. Porbahaie M, et al. 2023. PLoS One. 18:e0279626. PubMed
  138. Li H, et al. 2023. Clin Rheumatol. 42:1327. PubMed
  139. Freund EC, et al. 2023. Methods Mol Biol. 2618:201. PubMed
  140. Gao F, et al. 2023. Immunity. 56:864. PubMed
  141. Lütge M, et al. 2023. Nat Immunol. . PubMed
  142. Imai H, et al. 2023. iScience. 26:106822. PubMed
  143. Patskovsky Y, et al. 2023. Nat Commun. 14:3763. PubMed
  144. Cao B, et al. 2022. Nat Commun. 13:6203. PubMed
  145. Soday L, et al. 2021. Frontiers in Immunology. 12:600056. PubMed
  146. Lin L, et al. 2020. NAR Genom Bioinform. 2:lqaa059. PubMed
  147. Carre C, et al. 2021. iScience. 24:102970. PubMed
  148. Jiang G, et al. 2020. Aging (Albany NY). 12:11466. PubMed
  149. Bhattacharya S, et al. 2020. Front Bioeng Biotechnol. 1.055555556. PubMed
  150. Barrow AD et al. 2018. Cell. 172(3):534-548 . PubMed
  151. Ye Y, et al. 2022. Nat Commun. 13:6458. PubMed
  152. Li C, et al. 2022. J Exp Med. 219:. PubMed
  153. Netsrithong R, et al. 2020. Stem Cell Research. 39:101487.. PubMed
  154. Rendeiro AF, et al. 2020. Nat Commun. 11:577. PubMed
  155. Ryzhov S, et al. 2019. J Am Heart Assoc. 8:e010874. PubMed
  156. Klemm F, et al. 2020. Cell. 181(7):1643-1660.e17. PubMed
  157. Chen L, et al. 2018. Immunology. 155:123. PubMed
  158. Kimura I, et al. 2022. Cell Rep. 38:110218. PubMed
  159. Chen M, et al. 2021. Cancers (Basel). 13:. PubMed
  160. Laustsen A, et al. 2021. Elife. 10:. PubMed
  161. Pholcharee T, et al. 2021. Nat Commun. 12:1063. PubMed
  162. Rolandelli A, et al. 2017. Sci Rep. 7:40666. PubMed
  163. Karlsson H, et al. 2015. PLoS One. 10: 0144787. PubMed
  164. Eri Yamada et al. 2018. Cell host & microbe. 23(1):110-120 . PubMed
  165. Ruetsche D, et al. 2022. Cells. 11:. PubMed
  166. Esaulova E, et al. 2020. Cell Host Microbe. 29(2):165-178.e8. PubMed
  167. Liu C, et al. 2021. Cell Res. 31:1106. PubMed
  168. Devalraju KP, et al. 2021. PLoS One. 16:e0257185. PubMed
  169. Engelmann C, et al. 2017. J Transl Med. 10.1186/s12967-017-1288-3. PubMed
  170. Zhan Y, et al. 2021. JCI Insight. 6:. PubMed
  171. Perelman SS, et al. 2021. Nat Microbiol. 6:731. PubMed
  172. Zirbes A, et al. 2021. J Mammary Gland Biol Neoplasia. 26:247. PubMed
  173. Jiang Y, et al. 2020. Clin Immunol. 218:108516. PubMed
  174. Bergamaschi L, et al. 2021. Immunity. 54(6):1257-1275.e8. PubMed
  175. Maritz MF, et al. 2018. PLoS One. 13:e0196043. PubMed
  176. Appios A, et al. 2021. Bio Protoc. 11:e4044. PubMed
  177. Yarmarkovich M, et al. 2021. Nature. 599:477. PubMed
  178. Shevyrev D, et al. 2021. Exp Ther Med. 209:21. PubMed
  179. Caduff N, et al. 2021. Cell Reports. 35(5):109056. PubMed
  180. Ping Y, et al. 2020. Front Cell Dev Biol. 0.890972222. PubMed
  181. Yuan Z, et al. 2018. Emerg Microbes Infect. 7:59. PubMed
  182. Ramos RN, et al. 2022. Clin Transl Immunology. 11:e1392. PubMed
  183. Tu HA, et al. 2020. Cell Rep Med. 1:100155. PubMed
  184. Corleis B, et al. 2017. PLoS One. 12:e0173161. PubMed
  185. Ray RM, et al. 2019. Sci Rep. 9:4334. PubMed
  186. Rajamanickam V, et al. 2021. Cancer Immunol Res. 9:602. PubMed
  187. Laroni A, et al. 2016. J Autoimmun. 72:8-18. PubMed
  188. Sawasdee N, et al. 2022. Int J Mol Med. 49:. PubMed
  189. Mishra HK, et al. 2021. Front Immunol. 12:711621. PubMed
  190. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  191. Minervina AA, et al. 2022. Nat Immunol. 23:781. PubMed
  192. Cai D, et al. 2020. Cell Mol Immunol. 17:227. PubMed
  193. Krishnan S, et al. 2021. Clin Exp Immunol. 203:458. PubMed
  194. Rossignol ED, et al. 2021. Cell Reports. 35(8):109167. PubMed
  195. Huang N, et al. 2020. Genome Biol. 1.03125. PubMed
  196. Coufal S, et al. 2019. Cells. 8. PubMed
  197. Jin S, et al. 2020. Cell Res. 30:950. PubMed
  198. Liu Y, et al. 2020. Methods Mol Biol. 2111:285. PubMed
  199. Whitfield SJC, et al. 2017. J Immunol. 198:3989. PubMed
  200. Cizmeci D, et al. 2021. eLife. 10:00. PubMed
  201. Platten M, et al. 2021. Nature. 592:463. PubMed
  202. Kariminia A, et al. 2016. Blood. 127: 3082 - 3091. PubMed
  203. Lima J, et al. 2016. Reprod Sci. 10.1177/1933719116653680. PubMed
  204. Meermeier E, et al. 2016. Nat Commun. 7:12506. PubMed
  205. Wang J, et al. 2019. Oncol Lett. 4.948611111. PubMed
  206. Kato M, et al. 2021. PLoS One. 16:e0252116. PubMed
  207. Bak S, et al. 2019. Front Immunol. 9:2953. PubMed
  208. Setliff I, et al. 2018. Cell Host Microbe. 23:845. PubMed
  209. Collins PL et al. 2018. Cell. 176(1-2):348-360 . PubMed
  210. Cecotto L, et al. 2022. iScience. 25:105211. PubMed
  211. Shin CH, et al. 2020. J Immunol. 205:3179. PubMed
  212. Hakki S, et al. 2022. Sci Rep. 12:1427. PubMed
  213. Chapelin F, et al. 2017. Sci Rep.. 10.1038/s41598-017-17669-4. PubMed
  214. Reuschl AK, et al. 2022. Cell Rep. 39:110650. PubMed
  215. Abe Y, et al. 2022. Nat Cell Biol. 24:565. PubMed
  216. Cipriano M, et al. 2022. Commun Biol. 5:52. PubMed
  217. Kaito Y, et al. 2022. Oncol Lett. 23:51. PubMed
  218. Wang Y, et al. 2021. Sci Adv. 7: . PubMed
  219. Cantoni C, et al. 2021. Oncoimmunology. 1879530:10. PubMed
  220. Motozono C, et al. 2021. Cell Host Microbe. . PubMed
  221. Capone S, et al. 2020. NPJ Vaccines. 5:94. PubMed
  222. Domanska D, et al. 2022. J Exp Med. 219:. PubMed
  223. van Erp EA, et al. 2020. Clin Transl Immunology. 9:e1112. PubMed
  224. Maas RR, et al. 2021. Nat Protoc. 16:4692. PubMed
  225. Duhen R, et al. 2021. Nat Commun. 12:1047. PubMed
  226. Wang Z, et al. 2021. Nat Commun. 12:1724. PubMed
  227. Nakatsugawa M, et al. 2015. J Immunol. 194 3487 . PubMed
  228. Nakatsugawa M, et al. 2016. Sci Rep. 6:23821. PubMed
  229. Tamai Y, et al. 2013. J Immunol. 190:4382. PubMed
  230. Beyer AI, et al. 2017. Stem Cells Dev. 26:102. PubMed
  231. Lee M, et al. 2022. Front Cell Dev Biol. 10:909557. PubMed
  232. Albrecht M, et al. 2022. Clin Transl Immunology. 11:e1398. PubMed
  233. Rambaldi B, et al. 2022. Haematologica. 107:2617. PubMed
  234. Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed
  235. Chen YP, et al. 2020. Cell Res. 30:1024. PubMed
  236. Dyson MR, et al. 2020. MAbs. 12:1829335. PubMed
  237. Ochi T, et al. 2015. Cancer Immunol Res. 3: 1070-1081. PubMed
  238. Suzuki D, et al. 2020. Stem Cell Reports. 14:49. PubMed
  239. Wang SQ, et al. 2021. J Viral Hepat. 28:795. PubMed
  240. Pappa V, et al. 2021. J Neuroimmunol. 353:577524. PubMed
  241. Vanoni G, et al. 2021. eLife. 10:00. PubMed
  242. Goc J, et al. 2021. Cell. 184:5015. PubMed
  243. Wang S, et al. 2022. Cancer Immunol Immunother. Online ahead of print. PubMed
  244. Schmidts A, et al. 2020. J Immunother Cancer. 8:. PubMed
  245. Bennstein SB, et al. 2020. eLife. 9:e55232.. PubMed
  246. Chang ZL, et al. 2020. Nature Protocols. 15(4):1507-1524.. PubMed
RRID
AB_314059 (BioLegend Cat. No. 300405)
AB_314060 (BioLegend Cat. No. 300406)
AB_2564148 (BioLegend Cat. No. 300452)
AB_2562046 (BioLegend Cat. No. 300440)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

There are no FAQs for this product.

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Brilliant Violet 785™ anti-human CD3 UCHT1 FC
PE/Dazzle™ 594 anti-human CD3 UCHT1 FC
TotalSeq™-A0034 anti-human CD3 UCHT1 PG
TotalSeq™-B0034 anti-human CD3 UCHT1 PG
TotalSeq™-C0034 anti-human CD3 UCHT1 PG
PE anti-human CD3 UCHT1 FC
PE/Cyanine7 anti-human CD3 UCHT1 FC
KIRAVIA Blue 520™ anti-human CD3 UCHT1 FC
APC anti-human CD3 UCHT1 FC
FITC anti-human CD3 UCHT1 FC
PE anti-human CD3 UCHT1 FC
PE/Cyanine5 anti-human CD3 UCHT1 FC
Biotin anti-human CD3 UCHT1 FC
Purified anti-human CD3 UCHT1 FC,CyTOF®,IHC-F,IP,Activ,WB
Alexa Fluor® 647 anti-human CD3 UCHT1 FC,ICC,IHC-F
PE/Cyanine7 anti-human CD3 UCHT1 FC
Alexa Fluor® 488 anti-human CD3 UCHT1 FC,ICC,IHC-F
Pacific Blue™ anti-human CD3 UCHT1 FC
Alexa Fluor® 700 anti-human CD3 UCHT1 FC
APC/Cyanine7 anti-human CD3 UCHT1 FC
PerCP/Cyanine5.5 anti-human CD3 UCHT1 FC
PerCP anti-human CD3 UCHT1 FC
Brilliant Violet 421™ anti-human CD3 UCHT1 FC,ICC,IHC-F
Brilliant Violet 570™ anti-human CD3 UCHT1 FC
Ultra-LEAF™ Purified anti-human CD3 UCHT1 FC,CyTOF®,IHC-F,IP,Activ,WB
Brilliant Violet 510™ anti-human CD3 UCHT1 FC
Purified anti-human CD3 (Maxpar® Ready) UCHT1 FC,CyTOF®
Alexa Fluor® 594 anti-human CD3 UCHT1 ICC,FC,SB
PE/Dazzle™ 594 anti-human CD3 UCHT1 FC
Spark Violet™ 538 anti-human CD3 Antibody UCHT1 FC
TotalSeq™-D0034 anti-human CD3 UCHT1 PG
Spark Blue™ 574 anti-human CD3 Antibody UCHT1 FC
GMP Pacific Blue™ anti-human CD3 UCHT1 FC
GMP PE anti-human CD3 UCHT1 FC
Spark Violet™ 423 anti-human CD3 UCHT1 FC
GMP PE/Dazzle™ 594 anti-human CD3 UCHT1 FC
GMP PE/Cyanine7 anti-human CD3 UCHT1 FC
Spark Blue™ 515 anti-human CD3 UCHT1 FC
PE/Fire™ 810 anti-human CD3 UCHT1 FC
Spark YG™ 581 anti-human CD3 (Flexi-Fluor™) UCHT1 FC
APC/Fire™ 810 anti-human CD3 UCHT1 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Pricing & Availability
Clone
UCHT1 (See other available formats)
Regulatory Status
RUO
Workshop
III 471
Other Names
T3, CD3ε
Isotype
Mouse IgG1, κ
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Product Citations
publications
UCHT1_FITC_021805
Human peripheral blood lymphocytes stained with UCHT1 FITC
  • UCHT1_FITC_021805
    Human peripheral blood lymphocytes stained with UCHT1 FITC
Compare all formats See FITC spectral data
Cat # Size Price Quantity Check Availability Save
300405 25 tests DKK131
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300406 100 tests DKK297
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300452 100 µg DKK321
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300440 500 tests DKK1212
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Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NKT cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
µg sizes: 0.5 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, Western blotting9, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Application References
  1. Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP)
  2. Graves J, et al. 1991. J. Immunol. 146:2102. (Activ)
  3. Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ)
  4. Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC)
  5. Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ)
  6. Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC)
  7. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  8. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  9. Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
  10. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  11. Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC)
  12. Luckashenak N, et al. 2013. J. Immunol. 190:27. PubMed
  13. Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed
  14. Li J, et al. 2015. Cancer Res. 75:508. PubMed
  15. Stoeckius M, et al. 2017. Nat. Methods. 14:865-868. (PG)
  16. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  17. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
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  2. Sajadi MM, et al. 2018. Cell. 173:1783. PubMed
  3. Qin L, et al. 2022. Sci Adv. 8:eabn3774. PubMed
  4. Gan X, et al. 2022. Proc Natl Acad Sci U S A. 119:e2200879119. PubMed
  5. Sušac L, et al. 2022. Cell. 185:3201. PubMed
  6. Saber MM, et al. 2022. J Immunol Res. 2022:7219207. PubMed
  7. McEvoy CM, et al. 2022. Nat Commun. 13:7634. PubMed
  8. Graciliano NG, et al. 2023. Front Immunol. 13:1031248. PubMed
  9. Sun W, et al. 2023. Nature. 614:309. PubMed
  10. Namvar N, et al. 2022. Iran J Allergy Asthma Immunol. 21:600. PubMed
  11. Zhong J, et al. 2023. Front Immunol. 13:1081889. PubMed
  12. Yen M, et al. 2022. Cell. 185:1414. PubMed
  13. Shang P, et al. 2022. Acta Biochim Biophys Sin (Shanghai). 54:1497. PubMed
  14. Porbahaie M, et al. 2023. PLoS One. 18:e0279626. PubMed
  15. Li H, et al. 2023. Clin Rheumatol. 42:1327. PubMed
  16. Freund EC, et al. 2023. Methods Mol Biol. 2618:201. PubMed
  17. Gao F, et al. 2023. Immunity. 56:864. PubMed
  18. Lütge M, et al. 2023. Nat Immunol. . PubMed
  19. Imai H, et al. 2023. iScience. 26:106822. PubMed
  20. Patskovsky Y, et al. 2023. Nat Commun. 14:3763. PubMed
  21. Cao B, et al. 2022. Nat Commun. 13:6203. PubMed
  22. Soday L, et al. 2021. Frontiers in Immunology. 12:600056. PubMed
  23. Lin L, et al. 2020. NAR Genom Bioinform. 2:lqaa059. PubMed
  24. Carre C, et al. 2021. iScience. 24:102970. PubMed
  25. Jiang G, et al. 2020. Aging (Albany NY). 12:11466. PubMed
  26. Bhattacharya S, et al. 2020. Front Bioeng Biotechnol. 1.055555556. PubMed
  27. Barrow AD et al. 2018. Cell. 172(3):534-548 . PubMed
  28. Ye Y, et al. 2022. Nat Commun. 13:6458. PubMed
  29. Li C, et al. 2022. J Exp Med. 219:. PubMed
  30. Netsrithong R, et al. 2020. Stem Cell Research. 39:101487.. PubMed
  31. Rendeiro AF, et al. 2020. Nat Commun. 11:577. PubMed
  32. Ryzhov S, et al. 2019. J Am Heart Assoc. 8:e010874. PubMed
  33. Klemm F, et al. 2020. Cell. 181(7):1643-1660.e17. PubMed
  34. Chen L, et al. 2018. Immunology. 155:123. PubMed
  35. Kimura I, et al. 2022. Cell Rep. 38:110218. PubMed
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  38. Pholcharee T, et al. 2021. Nat Commun. 12:1063. PubMed
  39. Rolandelli A, et al. 2017. Sci Rep. 7:40666. PubMed
  40. Karlsson H, et al. 2015. PLoS One. 10: 0144787. PubMed
  41. Eri Yamada et al. 2018. Cell host & microbe. 23(1):110-120 . PubMed
  42. Ruetsche D, et al. 2022. Cells. 11:. PubMed
  43. Esaulova E, et al. 2020. Cell Host Microbe. 29(2):165-178.e8. PubMed
  44. Liu C, et al. 2021. Cell Res. 31:1106. PubMed
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  49. Zirbes A, et al. 2021. J Mammary Gland Biol Neoplasia. 26:247. PubMed
  50. Jiang Y, et al. 2020. Clin Immunol. 218:108516. PubMed
  51. Bergamaschi L, et al. 2021. Immunity. 54(6):1257-1275.e8. PubMed
  52. Maritz MF, et al. 2018. PLoS One. 13:e0196043. PubMed
  53. Appios A, et al. 2021. Bio Protoc. 11:e4044. PubMed
  54. Yarmarkovich M, et al. 2021. Nature. 599:477. PubMed
  55. Shevyrev D, et al. 2021. Exp Ther Med. 209:21. PubMed
  56. Caduff N, et al. 2021. Cell Reports. 35(5):109056. PubMed
  57. Ping Y, et al. 2020. Front Cell Dev Biol. 0.890972222. PubMed
  58. Yuan Z, et al. 2018. Emerg Microbes Infect. 7:59. PubMed
  59. Ramos RN, et al. 2022. Clin Transl Immunology. 11:e1392. PubMed
  60. Tu HA, et al. 2020. Cell Rep Med. 1:100155. PubMed
  61. Corleis B, et al. 2017. PLoS One. 12:e0173161. PubMed
  62. Ray RM, et al. 2019. Sci Rep. 9:4334. PubMed
  63. Rajamanickam V, et al. 2021. Cancer Immunol Res. 9:602. PubMed
  64. Laroni A, et al. 2016. J Autoimmun. 72:8-18. PubMed
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  67. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  68. Minervina AA, et al. 2022. Nat Immunol. 23:781. PubMed
  69. Cai D, et al. 2020. Cell Mol Immunol. 17:227. PubMed
  70. Krishnan S, et al. 2021. Clin Exp Immunol. 203:458. PubMed
  71. Rossignol ED, et al. 2021. Cell Reports. 35(8):109167. PubMed
  72. Huang N, et al. 2020. Genome Biol. 1.03125. PubMed
  73. Coufal S, et al. 2019. Cells. 8. PubMed
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  75. Liu Y, et al. 2020. Methods Mol Biol. 2111:285. PubMed
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  125. Sajadi MM, et al. 2018. Cell. 173:1783. PubMed
  126. Qin L, et al. 2022. Sci Adv. 8:eabn3774. PubMed
  127. Gan X, et al. 2022. Proc Natl Acad Sci U S A. 119:e2200879119. PubMed
  128. Sušac L, et al. 2022. Cell. 185:3201. PubMed
  129. Saber MM, et al. 2022. J Immunol Res. 2022:7219207. PubMed
  130. McEvoy CM, et al. 2022. Nat Commun. 13:7634. PubMed
  131. Graciliano NG, et al. 2023. Front Immunol. 13:1031248. PubMed
  132. Sun W, et al. 2023. Nature. 614:309. PubMed
  133. Namvar N, et al. 2022. Iran J Allergy Asthma Immunol. 21:600. PubMed
  134. Zhong J, et al. 2023. Front Immunol. 13:1081889. PubMed
  135. Yen M, et al. 2022. Cell. 185:1414. PubMed
  136. Shang P, et al. 2022. Acta Biochim Biophys Sin (Shanghai). 54:1497. PubMed
  137. Porbahaie M, et al. 2023. PLoS One. 18:e0279626. PubMed
  138. Li H, et al. 2023. Clin Rheumatol. 42:1327. PubMed
  139. Freund EC, et al. 2023. Methods Mol Biol. 2618:201. PubMed
  140. Gao F, et al. 2023. Immunity. 56:864. PubMed
  141. Lütge M, et al. 2023. Nat Immunol. . PubMed
  142. Imai H, et al. 2023. iScience. 26:106822. PubMed
  143. Patskovsky Y, et al. 2023. Nat Commun. 14:3763. PubMed
  144. Cao B, et al. 2022. Nat Commun. 13:6203. PubMed
  145. Soday L, et al. 2021. Frontiers in Immunology. 12:600056. PubMed
  146. Lin L, et al. 2020. NAR Genom Bioinform. 2:lqaa059. PubMed
  147. Carre C, et al. 2021. iScience. 24:102970. PubMed
  148. Jiang G, et al. 2020. Aging (Albany NY). 12:11466. PubMed
  149. Bhattacharya S, et al. 2020. Front Bioeng Biotechnol. 1.055555556. PubMed
  150. Barrow AD et al. 2018. Cell. 172(3):534-548 . PubMed
  151. Ye Y, et al. 2022. Nat Commun. 13:6458. PubMed
  152. Li C, et al. 2022. J Exp Med. 219:. PubMed
  153. Netsrithong R, et al. 2020. Stem Cell Research. 39:101487.. PubMed
  154. Rendeiro AF, et al. 2020. Nat Commun. 11:577. PubMed
  155. Ryzhov S, et al. 2019. J Am Heart Assoc. 8:e010874. PubMed
  156. Klemm F, et al. 2020. Cell. 181(7):1643-1660.e17. PubMed
  157. Chen L, et al. 2018. Immunology. 155:123. PubMed
  158. Kimura I, et al. 2022. Cell Rep. 38:110218. PubMed
  159. Chen M, et al. 2021. Cancers (Basel). 13:. PubMed
  160. Laustsen A, et al. 2021. Elife. 10:. PubMed
  161. Pholcharee T, et al. 2021. Nat Commun. 12:1063. PubMed
  162. Rolandelli A, et al. 2017. Sci Rep. 7:40666. PubMed
  163. Karlsson H, et al. 2015. PLoS One. 10: 0144787. PubMed
  164. Eri Yamada et al. 2018. Cell host & microbe. 23(1):110-120 . PubMed
  165. Ruetsche D, et al. 2022. Cells. 11:. PubMed
  166. Esaulova E, et al. 2020. Cell Host Microbe. 29(2):165-178.e8. PubMed
  167. Liu C, et al. 2021. Cell Res. 31:1106. PubMed
  168. Devalraju KP, et al. 2021. PLoS One. 16:e0257185. PubMed
  169. Engelmann C, et al. 2017. J Transl Med. 10.1186/s12967-017-1288-3. PubMed
  170. Zhan Y, et al. 2021. JCI Insight. 6:. PubMed
  171. Perelman SS, et al. 2021. Nat Microbiol. 6:731. PubMed
  172. Zirbes A, et al. 2021. J Mammary Gland Biol Neoplasia. 26:247. PubMed
  173. Jiang Y, et al. 2020. Clin Immunol. 218:108516. PubMed
  174. Bergamaschi L, et al. 2021. Immunity. 54(6):1257-1275.e8. PubMed
  175. Maritz MF, et al. 2018. PLoS One. 13:e0196043. PubMed
  176. Appios A, et al. 2021. Bio Protoc. 11:e4044. PubMed
  177. Yarmarkovich M, et al. 2021. Nature. 599:477. PubMed
  178. Shevyrev D, et al. 2021. Exp Ther Med. 209:21. PubMed
  179. Caduff N, et al. 2021. Cell Reports. 35(5):109056. PubMed
  180. Ping Y, et al. 2020. Front Cell Dev Biol. 0.890972222. PubMed
  181. Yuan Z, et al. 2018. Emerg Microbes Infect. 7:59. PubMed
  182. Ramos RN, et al. 2022. Clin Transl Immunology. 11:e1392. PubMed
  183. Tu HA, et al. 2020. Cell Rep Med. 1:100155. PubMed
  184. Corleis B, et al. 2017. PLoS One. 12:e0173161. PubMed
  185. Ray RM, et al. 2019. Sci Rep. 9:4334. PubMed
  186. Rajamanickam V, et al. 2021. Cancer Immunol Res. 9:602. PubMed
  187. Laroni A, et al. 2016. J Autoimmun. 72:8-18. PubMed
  188. Sawasdee N, et al. 2022. Int J Mol Med. 49:. PubMed
  189. Mishra HK, et al. 2021. Front Immunol. 12:711621. PubMed
  190. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  191. Minervina AA, et al. 2022. Nat Immunol. 23:781. PubMed
  192. Cai D, et al. 2020. Cell Mol Immunol. 17:227. PubMed
  193. Krishnan S, et al. 2021. Clin Exp Immunol. 203:458. PubMed
  194. Rossignol ED, et al. 2021. Cell Reports. 35(8):109167. PubMed
  195. Huang N, et al. 2020. Genome Biol. 1.03125. PubMed
  196. Coufal S, et al. 2019. Cells. 8. PubMed
  197. Jin S, et al. 2020. Cell Res. 30:950. PubMed
  198. Liu Y, et al. 2020. Methods Mol Biol. 2111:285. PubMed
  199. Whitfield SJC, et al. 2017. J Immunol. 198:3989. PubMed
  200. Cizmeci D, et al. 2021. eLife. 10:00. PubMed
  201. Platten M, et al. 2021. Nature. 592:463. PubMed
  202. Kariminia A, et al. 2016. Blood. 127: 3082 - 3091. PubMed
  203. Lima J, et al. 2016. Reprod Sci. 10.1177/1933719116653680. PubMed
  204. Meermeier E, et al. 2016. Nat Commun. 7:12506. PubMed
  205. Wang J, et al. 2019. Oncol Lett. 4.948611111. PubMed
  206. Kato M, et al. 2021. PLoS One. 16:e0252116. PubMed
  207. Bak S, et al. 2019. Front Immunol. 9:2953. PubMed
  208. Setliff I, et al. 2018. Cell Host Microbe. 23:845. PubMed
  209. Collins PL et al. 2018. Cell. 176(1-2):348-360 . PubMed
  210. Cecotto L, et al. 2022. iScience. 25:105211. PubMed
  211. Shin CH, et al. 2020. J Immunol. 205:3179. PubMed
  212. Hakki S, et al. 2022. Sci Rep. 12:1427. PubMed
  213. Chapelin F, et al. 2017. Sci Rep.. 10.1038/s41598-017-17669-4. PubMed
  214. Reuschl AK, et al. 2022. Cell Rep. 39:110650. PubMed
  215. Abe Y, et al. 2022. Nat Cell Biol. 24:565. PubMed
  216. Cipriano M, et al. 2022. Commun Biol. 5:52. PubMed
  217. Kaito Y, et al. 2022. Oncol Lett. 23:51. PubMed
  218. Wang Y, et al. 2021. Sci Adv. 7: . PubMed
  219. Cantoni C, et al. 2021. Oncoimmunology. 1879530:10. PubMed
  220. Motozono C, et al. 2021. Cell Host Microbe. . PubMed
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RRID
AB_314059 (BioLegend Cat. No. 300405)
AB_314060 (BioLegend Cat. No. 300406)
AB_2564148 (BioLegend Cat. No. 300452)
AB_2562046 (BioLegend Cat. No. 300440)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Other Formats

View All CD3 Reagents Request Custom Conjugation
Description Clone Applications
Brilliant Violet 605™ anti-human CD3 UCHT1 FC
Brilliant Violet 711™ anti-human CD3 UCHT1 FC
Brilliant Violet 650™ anti-human CD3 UCHT1 FC
APC/Fire™ 750 anti-human CD3 UCHT1 FC
Pacific Blue™ anti-human CD3 UCHT1 FC
Brilliant Violet 785™ anti-human CD3 UCHT1 FC
PE/Dazzle™ 594 anti-human CD3 UCHT1 FC
TotalSeq™-A0034 anti-human CD3 UCHT1 PG
TotalSeq™-B0034 anti-human CD3 UCHT1 PG
TotalSeq™-C0034 anti-human CD3 UCHT1 PG
PE anti-human CD3 UCHT1 FC
PE/Cyanine7 anti-human CD3 UCHT1 FC
KIRAVIA Blue 520™ anti-human CD3 UCHT1 FC
APC anti-human CD3 UCHT1 FC
FITC anti-human CD3 UCHT1 FC
PE anti-human CD3 UCHT1 FC
PE/Cyanine5 anti-human CD3 UCHT1 FC
Biotin anti-human CD3 UCHT1 FC
Purified anti-human CD3 UCHT1 FC,CyTOF®,IHC-F,IP,Activ,WB
Alexa Fluor® 647 anti-human CD3 UCHT1 FC,ICC,IHC-F
PE/Cyanine7 anti-human CD3 UCHT1 FC
Alexa Fluor® 488 anti-human CD3 UCHT1 FC,ICC,IHC-F
Pacific Blue™ anti-human CD3 UCHT1 FC
Alexa Fluor® 700 anti-human CD3 UCHT1 FC
APC/Cyanine7 anti-human CD3 UCHT1 FC
PerCP/Cyanine5.5 anti-human CD3 UCHT1 FC
PerCP anti-human CD3 UCHT1 FC
Brilliant Violet 421™ anti-human CD3 UCHT1 FC,ICC,IHC-F
Brilliant Violet 570™ anti-human CD3 UCHT1 FC
Ultra-LEAF™ Purified anti-human CD3 UCHT1 FC,CyTOF®,IHC-F,IP,Activ,WB
Brilliant Violet 510™ anti-human CD3 UCHT1 FC
Purified anti-human CD3 (Maxpar® Ready) UCHT1 FC,CyTOF®
Alexa Fluor® 594 anti-human CD3 UCHT1 ICC,FC,SB
PE/Dazzle™ 594 anti-human CD3 UCHT1 FC
Spark Violet™ 538 anti-human CD3 Antibody UCHT1 FC
TotalSeq™-D0034 anti-human CD3 UCHT1 PG
Spark Blue™ 574 anti-human CD3 Antibody UCHT1 FC
GMP Pacific Blue™ anti-human CD3 UCHT1 FC
GMP PE anti-human CD3 UCHT1 FC
Spark Violet™ 423 anti-human CD3 UCHT1 FC
GMP PE/Dazzle™ 594 anti-human CD3 UCHT1 FC
GMP PE/Cyanine7 anti-human CD3 UCHT1 FC
Spark Blue™ 515 anti-human CD3 UCHT1 FC
PE/Fire™ 810 anti-human CD3 UCHT1 FC
Spark YG™ 581 anti-human CD3 (Flexi-Fluor™) UCHT1 FC
APC/Fire™ 810 anti-human CD3 UCHT1 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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