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FITC anti-mouse I-A/I-E Antibody FITC anti-mouse I-A/I-E Antibody

Pricing & Availability
Clone
M5/114.15.2 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class II
Isotype
Rat IgG2b, κ
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Product Citations
publications
m5/114.15.2 FITC
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2) FITC (solid line) or rat IgG2b, κ FITC isotype control (dashed line).
  • m5/114.15.2 FITC
    C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2) FITC (solid line) or rat IgG2b, κ FITC isotype control (dashed line).
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Cat # Size Price Quantity Check Availability Save
107605 50 µg DKK422
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107606 500 µg DKK1307
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Description

These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Activated C57BL/6 mouse spleen cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens from mice carrying H-2p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-As MHC class II alloantigens.1

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6, in vitro and in vivo blocking of antigen presentation or ligand binding4-7, and spatial biology (IBEX)17,18. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).

Application References
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  2. Viville S, et al. 1993. Cell 72:635. (IHC)
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  6. Guo M, et al. 1995. Zygote 3:65. (IHC)
  7. Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
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  18. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
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  39. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
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  41. Matsumura F, et al. 2021. J Immunol. 207:938. PubMed
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  46. Sultan H, et al. 2018. Cancer Immunol Res. 6:617. PubMed
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  54. Kimura A, et al. 2018. J Exp Med. 215:2197. PubMed
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  56. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  57. Wang L, et al. 2021. Cancer Immunol Res. 9:348. PubMed
  58. Enriquez AB, et al. 2022. iScience. 25:104305. PubMed
  59. Mainz RE, et al. 2022. Cells. 11:. PubMed
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  61. Zhang P, et al. 2022. BMC Med. 20:435. PubMed
  62. Kang SJ, et al. 2022. Food Sci Anim Resour. 42:903. PubMed
  63. Carozza JA, et al. 2020. Nat Cancer. 184:1. PubMed
  64. Wang Y, et al. 2021. Sci Rep. 1.429861111. PubMed
  65. Reynolds A, et al. 2015. J Immunol. 194:231. PubMed
  66. Morris E, et al. 2005. J Clin Invest. 115:3093. PubMed
  67. Bertino P, et al. 2019. Oncoimmunology. 8:1601482. PubMed
  68. Tsukasaki M, et al. 2020. Cell Reports. 32(10):108124. PubMed
  69. Hernández–Santos N, et al. 2018. Cell Host Microbe. 1.313194444. PubMed
  70. Kaplan D, et al. 2007. J Exp Med. 204:2545. PubMed
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  73. Zou J, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:983. PubMed
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  75. Dobosz E, et al. 2021. Dis Model Mech. :14. PubMed
  76. Bayik D, et al. 2020. Cancer Discov. 1.256944444. PubMed
  77. Koma T, et al. 2014. J Virol. 88:7178. PubMed
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  79. Zhang J, et al. 2018. Sci Rep. 8:2373. PubMed
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  90. Maisel D, et al. 2016. PLoS One. 11: 0159716. PubMed
  91. Steinbach K, et al. 2013. J Immunol. 191:4531. PubMed
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  100. Zwick M, et al. 2019. Front Immunol. 10:222. PubMed
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  103. Luo M, et al. 2021. J Immunol. 207:1683. PubMed
  104. Kelly SH, et al. 2021. Adv Healthc Mater. 10:e2001614. PubMed
  105. Bagati A, et al. 2020. Cancer Cell. 39(1):54-67.e9. PubMed
  106. Moufarrij S, et al. 2020. Sci Rep. 10:3470. PubMed
  107. Wu J, et al. 2019. J Immunol Res. 2019:1749803. PubMed
  108. Wijayadikusumah A, et al. 2017. Amino Acids. 10.1007/s00726-017-2466-6. PubMed
  109. Sivapatham S, et al. 2019. Front Immunol. 1.865277778. PubMed
  110. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  111. Xiao S, Manley N 2010. PLoS One. 5:e15396. PubMed
  112. Schulz A, et al. 2015. J Biol Chem. 211: 553 - 567. PubMed
  113. Kramer K, et al. 2017. Mol Ther. 25(1):62-70. PubMed
  114. Grube J, et al. 2022. Cell Death Dis. 13:704. PubMed
  115. Chryplewicz A, et al. 2022. Cancer Cell. 40:1111. PubMed
  116. Xiao Z, et al. 2022. Mater Today Bio. 15:100297. PubMed
  117. Nicholls J, et al. 2021. Front Cell Dev Biol. 9:737880. PubMed
  118. Chetty A, et al. 2021. Cell Host Microbe. 29:579. PubMed
  119. Aguilera TA, et al. 2020. Clin Cancer Res. 26:2972. PubMed
  120. Danne C et al. 2017. Cell host & microbe. 22(6):733-745 . PubMed
  121. Arima Y et al. 2017. eLife. 6 pii: e25517. PubMed
  122. Hong Y, et al. 2019. J Extracell Vesicles. 8:1670893. PubMed
  123. Krotova K, et al. 2019. Mol Ther Oncolytics. 15:166. PubMed
  124. Steinmann S, et al. 2020. Sci Rep. 1.160416667. PubMed
  125. Stack G, et al. 2015. PLoS Pathog. 11:1004641. PubMed
  126. Harberts E, et al. 2015. J Immunol. 194:3127. PubMed
  127. Tran S, et al. 2020. Immunity. 53(3):627-640.e5. PubMed
  128. Al-Zaeed N, et al. 2021. Cell Death Dis. 12:611. PubMed
  129. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  130. Deets KA, et al. 2021. Elife. 10:. PubMed
  131. Spanier J, et al. 2016. Nat Commun. 7:11804. PubMed
  132. Dong S, et al. 2007. Int Immunopharmacol. 7:725. PubMed
  133. Park CJ, et al. 2020. Cell Reports. 31(2):107496.. PubMed
  134. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  135. Nguyen T, et al. 2021. STAR Protoc. 2:101004. PubMed
  136. Wang S, et al. 2022. Sci Adv. 8:eabn3883. PubMed
  137. Gargaro M, et al. 2022. Immunity. 55:1032. PubMed
  138. Mugarza E, et al. 2022. Sci Adv. 8:eabm8780. PubMed
  139. Que W, et al. 2022. Sci Adv. 8:eabo4413. PubMed
  140. Montilla A, et al. 2023. Cell Death Dis. 14:16. PubMed
  141. Tanaka T, et al. 2023. Nat Immunol. 24:439. PubMed
  142. Drummer C, et al. 2023. Front Immunol. 14:1113883. PubMed
  143. Yang Z, et al. 2022. J Nucl Med. 63:1708. PubMed
  144. Lopez R, et al. 2022. Nat Biotechnol. 40:1360. PubMed
  145. Reticker-Flynn NE, et al. 2022. Cell. 185:1924. PubMed
  146. Sheng YH, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:789. PubMed
  147. Qiu Z, et al. 2022. J Exp Med. 219:. PubMed
  148. Sauter M, et al. 2022. STAR Protoc. 3:101664. PubMed
  149. Pagni RL, et al. 2022. Front Immunol. 13:1005937. PubMed
  150. Chen X, et al. 2023. Adv Mater. 35:e2210440. PubMed
  151. Zhao Q, et al. 2023. Signal Transduct Target Ther. 8:40. PubMed
  152. Do-Thi VA, et al. 2023. Cancer Res Commun. 3:80. PubMed
  153. Guo Z, et al. 2023. Int J Mol Sci. 24:. PubMed
  154. Yao H, et al. 2023. Front Immunol. 14:1147674. PubMed
  155. Shen Z, et al. 2023. iScience. 26:106572. PubMed
  156. He Y, et al. 2023. Biomolecules. 13:. PubMed
  157. Hsieh MS, et al. 2023. NPJ Vaccines. 8:82. PubMed
  158. Yao RQ, et al. 2023. Mil Med Res. 10:27. PubMed
  159. Sim SL, et al. 2022. Front Endocrinol (Lausanne). 13:957833. PubMed
  160. Tarban N, et al. 2022. Cells. 11:. PubMed
  161. Jong RM, et al. 2022. J Immunol. 208:407. PubMed
  162. Ma J, et al. 2020. Adv Sci (Weinh). 7:2000609. PubMed
  163. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  164. Pierini S, et al. 2021. J Immunother Cancer. 9:. PubMed
  165. Du J, et al. 2020. Developmental Cell. 55(6):737-753.e7. PubMed
  166. Orillion A, et al. 2018. Clin Cancer Res. 24:6383. PubMed
  167. Mulder R, et al. 2017. Front Immunol. 1.464583333. PubMed
  168. Misumi I et al. 2019. Cell Rep. 27(5):1387-1396 . PubMed
  169. Nakamura M, et al. 2015. J Immunol. 194:243. PubMed
  170. Aguilera T, et al. 2016. Nat Commun. 7:13898. PubMed
  171. Hickey A, et al. 2021. Front Microbiol. 12:653587. PubMed
  172. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  173. Sauter M, et al. 2022. iScience. 25:103677. PubMed
  174. Matsumura F, et al. 2021. J Immunol. 207:938. PubMed
  175. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  176. Dutta S, et al. 2012. Eukaryot Cell. 0.756944444. PubMed
  177. He Y, et al. 2020. Sci Adv. 6:eaba7589. PubMed
  178. Hays C, et al. 2020. eLife. 8:e48772.. PubMed
  179. Sultan H, et al. 2018. Cancer Immunol Res. 6:617. PubMed
  180. Zhao J, et al. 2019. Nat Commun. 10:899. PubMed
  181. Zhu B, et al. 2021. Immunity. 54(6):1200-1218.e9. PubMed
  182. Enriquez AB, et al. 2022. iScience. 25:104305. PubMed
  183. Renga G, et al. 2020. Life Sci Alliance. 3:. PubMed
  184. Xu X, et al. 2021. J Nanobiotechnology. 19:376. PubMed
  185. Ayasoufi K, et al. 2020. Brain. 3629:143. PubMed
  186. Wang H et al. 2017. Developmental cell. 40(6):566-582 . PubMed
  187. Kimura A, et al. 2018. J Exp Med. 215:2197. PubMed
  188. Qu J, et al. 2018. J Immunol Res. 2018:7519856. PubMed
  189. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  190. Wang L, et al. 2021. Cancer Immunol Res. 9:348. PubMed
  191. Enriquez AB, et al. 2022. iScience. 25:104305. PubMed
  192. Mainz RE, et al. 2022. Cells. 11:. PubMed
  193. Zhao F, et al. 2022. Front Immunol. 13:873720. PubMed
  194. Zhang P, et al. 2022. BMC Med. 20:435. PubMed
  195. Kang SJ, et al. 2022. Food Sci Anim Resour. 42:903. PubMed
  196. Carozza JA, et al. 2020. Nat Cancer. 184:1. PubMed
  197. Wang Y, et al. 2021. Sci Rep. 1.429861111. PubMed
  198. Reynolds A, et al. 2015. J Immunol. 194:231. PubMed
  199. Morris E, et al. 2005. J Clin Invest. 115:3093. PubMed
  200. Bertino P, et al. 2019. Oncoimmunology. 8:1601482. PubMed
  201. Tsukasaki M, et al. 2020. Cell Reports. 32(10):108124. PubMed
  202. Hernández–Santos N, et al. 2018. Cell Host Microbe. 1.313194444. PubMed
  203. Kaplan D, et al. 2007. J Exp Med. 204:2545. PubMed
  204. Miki Y, et al. 2016. J Biol Chem. 291: 15588 - 15601. PubMed
  205. Alikhanyan K, et al. 2021. Cancers (Basel). 13:. PubMed
  206. Zou J, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:983. PubMed
  207. Pradhan K, et al. 2021. Front Immunol. 12:778830. PubMed
  208. Dobosz E, et al. 2021. Dis Model Mech. :14. PubMed
  209. Bayik D, et al. 2020. Cancer Discov. 1.256944444. PubMed
  210. Koma T, et al. 2014. J Virol. 88:7178. PubMed
  211. Donaldson B, et al. 2017. J Immunother Cancer. 10.1186/s40425-017-0270-1. PubMed
  212. Zhang J, et al. 2018. Sci Rep. 8:2373. PubMed
  213. Hakim R, et al. 2019. Stem Cell Res Ther. 10:115. PubMed
  214. Kramer K, et al. 2017. ACS Omega. 0.240972222. PubMed
  215. White C, et al. 2015. J Immunol. 194:697. PubMed
  216. Engler AE, et al. 2020. Cell Reports. 33(13):108553. PubMed
  217. Kim SH, et al. 2021. Cell Reports. 35(2):108995. PubMed
  218. Vorselen D, et al. 2021. Elife. 10:. PubMed
  219. Otvos B, et al. 2021. Clin Cancer Res. 27:2038. PubMed
  220. Maulloo CD, et al. 2021. Front Immunol. 12:714842. PubMed
  221. Koikawa K, et al. 2021. Cell. 184:4753. PubMed
  222. Dewi IMW, et al. 2021. Cell Reports Medicine. 2(5):100289. PubMed
  223. Maisel D, et al. 2016. PLoS One. 11: 0159716. PubMed
  224. Steinbach K, et al. 2013. J Immunol. 191:4531. PubMed
  225. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  226. Van Dis E, et al. 2018. Cell Rep. 23:1435. PubMed
  227. Guo S, et al. 2021. Sci Rep. 11:23745. PubMed
  228. Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed
  229. Ghorbani S, et al. 2022. Nat Commun. 13:2445. PubMed
  230. Zhao L, et al. 2021. STAR Protocols. 2:100732. PubMed
  231. Kang J, Lee J, Chang J 2016. PLoS One. 11: 0157015. PubMed
  232. Banovic T, et al. 2009. J Immunol. 182:912. PubMed
  233. Zwick M, et al. 2019. Front Immunol. 10:222. PubMed
  234. Hidaka T, et al. 2016. Nat Immunol. 18:64-73. PubMed
  235. Budai Z, et al. 2021. Cells. 10:. PubMed
  236. Luo M, et al. 2021. J Immunol. 207:1683. PubMed
  237. Kelly SH, et al. 2021. Adv Healthc Mater. 10:e2001614. PubMed
  238. Bagati A, et al. 2020. Cancer Cell. 39(1):54-67.e9. PubMed
  239. Moufarrij S, et al. 2020. Sci Rep. 10:3470. PubMed
  240. Wu J, et al. 2019. J Immunol Res. 2019:1749803. PubMed
  241. Wijayadikusumah A, et al. 2017. Amino Acids. 10.1007/s00726-017-2466-6. PubMed
  242. Sivapatham S, et al. 2019. Front Immunol. 1.865277778. PubMed
  243. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  244. Xiao S, Manley N 2010. PLoS One. 5:e15396. PubMed
  245. Schulz A, et al. 2015. J Biol Chem. 211: 553 - 567. PubMed
  246. Kramer K, et al. 2017. Mol Ther. 25(1):62-70. PubMed
  247. Grube J, et al. 2022. Cell Death Dis. 13:704. PubMed
  248. Chryplewicz A, et al. 2022. Cancer Cell. 40:1111. PubMed
  249. Xiao Z, et al. 2022. Mater Today Bio. 15:100297. PubMed
  250. Nicholls J, et al. 2021. Front Cell Dev Biol. 9:737880. PubMed
  251. Chetty A, et al. 2021. Cell Host Microbe. 29:579. PubMed
  252. Aguilera TA, et al. 2020. Clin Cancer Res. 26:2972. PubMed
  253. Danne C et al. 2017. Cell host & microbe. 22(6):733-745 . PubMed
  254. Arima Y et al. 2017. eLife. 6 pii: e25517. PubMed
  255. Hong Y, et al. 2019. J Extracell Vesicles. 8:1670893. PubMed
  256. Krotova K, et al. 2019. Mol Ther Oncolytics. 15:166. PubMed
  257. Steinmann S, et al. 2020. Sci Rep. 1.160416667. PubMed
  258. Stack G, et al. 2015. PLoS Pathog. 11:1004641. PubMed
  259. Harberts E, et al. 2015. J Immunol. 194:3127. PubMed
  260. Tran S, et al. 2020. Immunity. 53(3):627-640.e5. PubMed
  261. Al-Zaeed N, et al. 2021. Cell Death Dis. 12:611. PubMed
  262. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  263. Deets KA, et al. 2021. Elife. 10:. PubMed
  264. Spanier J, et al. 2016. Nat Commun. 7:11804. PubMed
  265. Dong S, et al. 2007. Int Immunopharmacol. 7:725. PubMed
  266. Park CJ, et al. 2020. Cell Reports. 31(2):107496.. PubMed
RRID
AB_313320 (BioLegend Cat. No. 107605)
AB_313321 (BioLegend Cat. No. 107606)

Antigen Details

Structure
MHC class II
Distribution

B cell and activated T cells, APCs of the H-2b,d,q,r bearing mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14961 View all products for this Gene ID 14969 View all products for this Gene ID
UniProt
View information about I-A/I-E on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All I-A/I-E Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse I-A/I-E (Maxpar® Ready) M5/114.15.2 FC,CyTOF®
Brilliant Violet 605™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 650™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 711™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 785™ anti-mouse I-A/I-E M5/114.15.2 FC
PE/Dazzle™ 594 anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 594 anti-mouse I-A/I-E M5/114.15.2 IHC-F,3D IHC
APC/Fire™ 750 anti-mouse I-A/I-E M5/114.15.2 FC
TotalSeq™-A0117 anti-mouse I-A/I-E M5/114.15.2 PG
Ultra-LEAF™ Purified anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,IP,Block
TotalSeq™-B0117 anti-mouse I-A/I-E M5/114.15.2 PG
TotalSeq™-C0117 anti-mouse I-A/I-E M5/114.15.2 PG
Spark Blue™ 550 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Fire™ 640 anti-mouse I-A/I-E M5/114.15.2 FC
Biotin anti-mouse I-A/I-E M5/114.15.2 FC
FITC anti-mouse I-A/I-E M5/114.15.2 FC
PE anti-mouse I-A/I-E M5/114.15.2 FC
Purified anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,IP,Block
PE/Cyanine5 anti-mouse I-A/I-E M5/114.15.2 FC
APC anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 488 anti-mouse I-A/I-E M5/114.15.2 FC,3D IHC
Alexa Fluor® 647 anti-mouse I-A/I-E M5/114.15.2 FC
Pacific Blue™ anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 700 anti-mouse I-A/I-E M5/114.15.2 FC,SB
PerCP/Cyanine5.5 anti-mouse I-A/I-E M5/114.15.2 FC
PerCP anti-mouse I-A/I-E M5/114.15.2 FC
APC/Cyanine7 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Cyanine7 anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 421™ anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,SB
Brilliant Violet 510™ anti-mouse I-A/I-E M5/114.15.2 FC
Spark YG™ 581 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Fire™ 810 anti-mouse I-A/I-E M5/114.15.2 FC
Spark UV™ 387 anti-mouse I-A/I-E M5/114.15.2 FC
Spark Violet™ 538 anti-mouse I-A/I-E M5/114.15.2 FC
PerCP/Fire™ 806 anti-mouse I-A/I-E M5/114.15.2 FC
APC/Fire™ 810 anti-mouse I-A/I-E M5/114.15.2 FC
Spark Red™ 718 anti-mouse I-A/I-E M5/114.15.2 FC
Spark PLUS UV395™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 750™ anti-mouse I-A/I-E M5/114.15.2 FC
Spark YG™ 593 anti-mouse I-A/I-E (Flexi-Fluor™) Antibody M5/114.15.2 FC
Spark NIR™ 685 anti-mouse I-A/I-E (Flexi-Fluor™) Antibody M5/114.15.2 FC
StarBright UltraViolet 795 anti-mouse I-A/I-E M5/114.15.2 FC
Spark PLUS B574™ anti-mouse I-A/I-E Antibody M5/114.15.2 FC
Go To Top Version: 4    Revision Date: 10/30/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Pricing & Availability
Clone
M5/114.15.2 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class II
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
m5/114.15.2 FITC
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2) FITC (solid line) or rat IgG2b, κ FITC isotype control (dashed line).
  • m5/114.15.2 FITC
    C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2) FITC (solid line) or rat IgG2b, κ FITC isotype control (dashed line).
Compare all formats See FITC spectral data
Cat # Size Price Quantity Check Availability Save
107605 50 µg DKK422
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107606 500 µg DKK1307
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Description

These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Activated C57BL/6 mouse spleen cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens from mice carrying H-2p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-As MHC class II alloantigens.1

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6, in vitro and in vivo blocking of antigen presentation or ligand binding4-7, and spatial biology (IBEX)17,18. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).

Application References
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  6. Guo M, et al. 1995. Zygote 3:65. (IHC)
  7. Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
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  16. Fu H, et al. 2014. Nat Commun. 5:3436. PubMed
  17. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  18. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
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  26. Sim SL, et al. 2022. Front Endocrinol (Lausanne). 13:957833. PubMed
  27. Tarban N, et al. 2022. Cells. 11:. PubMed
  28. Jong RM, et al. 2022. J Immunol. 208:407. PubMed
  29. Ma J, et al. 2020. Adv Sci (Weinh). 7:2000609. PubMed
  30. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
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  33. Orillion A, et al. 2018. Clin Cancer Res. 24:6383. PubMed
  34. Mulder R, et al. 2017. Front Immunol. 1.464583333. PubMed
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  36. Nakamura M, et al. 2015. J Immunol. 194:243. PubMed
  37. Aguilera T, et al. 2016. Nat Commun. 7:13898. PubMed
  38. Hickey A, et al. 2021. Front Microbiol. 12:653587. PubMed
  39. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  40. Sauter M, et al. 2022. iScience. 25:103677. PubMed
  41. Matsumura F, et al. 2021. J Immunol. 207:938. PubMed
  42. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  43. Dutta S, et al. 2012. Eukaryot Cell. 0.756944444. PubMed
  44. He Y, et al. 2020. Sci Adv. 6:eaba7589. PubMed
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  46. Sultan H, et al. 2018. Cancer Immunol Res. 6:617. PubMed
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  137. Gargaro M, et al. 2022. Immunity. 55:1032. PubMed
  138. Mugarza E, et al. 2022. Sci Adv. 8:eabm8780. PubMed
  139. Que W, et al. 2022. Sci Adv. 8:eabo4413. PubMed
  140. Montilla A, et al. 2023. Cell Death Dis. 14:16. PubMed
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  142. Drummer C, et al. 2023. Front Immunol. 14:1113883. PubMed
  143. Yang Z, et al. 2022. J Nucl Med. 63:1708. PubMed
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  145. Reticker-Flynn NE, et al. 2022. Cell. 185:1924. PubMed
  146. Sheng YH, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:789. PubMed
  147. Qiu Z, et al. 2022. J Exp Med. 219:. PubMed
  148. Sauter M, et al. 2022. STAR Protoc. 3:101664. PubMed
  149. Pagni RL, et al. 2022. Front Immunol. 13:1005937. PubMed
  150. Chen X, et al. 2023. Adv Mater. 35:e2210440. PubMed
  151. Zhao Q, et al. 2023. Signal Transduct Target Ther. 8:40. PubMed
  152. Do-Thi VA, et al. 2023. Cancer Res Commun. 3:80. PubMed
  153. Guo Z, et al. 2023. Int J Mol Sci. 24:. PubMed
  154. Yao H, et al. 2023. Front Immunol. 14:1147674. PubMed
  155. Shen Z, et al. 2023. iScience. 26:106572. PubMed
  156. He Y, et al. 2023. Biomolecules. 13:. PubMed
  157. Hsieh MS, et al. 2023. NPJ Vaccines. 8:82. PubMed
  158. Yao RQ, et al. 2023. Mil Med Res. 10:27. PubMed
  159. Sim SL, et al. 2022. Front Endocrinol (Lausanne). 13:957833. PubMed
  160. Tarban N, et al. 2022. Cells. 11:. PubMed
  161. Jong RM, et al. 2022. J Immunol. 208:407. PubMed
  162. Ma J, et al. 2020. Adv Sci (Weinh). 7:2000609. PubMed
  163. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  164. Pierini S, et al. 2021. J Immunother Cancer. 9:. PubMed
  165. Du J, et al. 2020. Developmental Cell. 55(6):737-753.e7. PubMed
  166. Orillion A, et al. 2018. Clin Cancer Res. 24:6383. PubMed
  167. Mulder R, et al. 2017. Front Immunol. 1.464583333. PubMed
  168. Misumi I et al. 2019. Cell Rep. 27(5):1387-1396 . PubMed
  169. Nakamura M, et al. 2015. J Immunol. 194:243. PubMed
  170. Aguilera T, et al. 2016. Nat Commun. 7:13898. PubMed
  171. Hickey A, et al. 2021. Front Microbiol. 12:653587. PubMed
  172. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  173. Sauter M, et al. 2022. iScience. 25:103677. PubMed
  174. Matsumura F, et al. 2021. J Immunol. 207:938. PubMed
  175. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  176. Dutta S, et al. 2012. Eukaryot Cell. 0.756944444. PubMed
  177. He Y, et al. 2020. Sci Adv. 6:eaba7589. PubMed
  178. Hays C, et al. 2020. eLife. 8:e48772.. PubMed
  179. Sultan H, et al. 2018. Cancer Immunol Res. 6:617. PubMed
  180. Zhao J, et al. 2019. Nat Commun. 10:899. PubMed
  181. Zhu B, et al. 2021. Immunity. 54(6):1200-1218.e9. PubMed
  182. Enriquez AB, et al. 2022. iScience. 25:104305. PubMed
  183. Renga G, et al. 2020. Life Sci Alliance. 3:. PubMed
  184. Xu X, et al. 2021. J Nanobiotechnology. 19:376. PubMed
  185. Ayasoufi K, et al. 2020. Brain. 3629:143. PubMed
  186. Wang H et al. 2017. Developmental cell. 40(6):566-582 . PubMed
  187. Kimura A, et al. 2018. J Exp Med. 215:2197. PubMed
  188. Qu J, et al. 2018. J Immunol Res. 2018:7519856. PubMed
  189. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  190. Wang L, et al. 2021. Cancer Immunol Res. 9:348. PubMed
  191. Enriquez AB, et al. 2022. iScience. 25:104305. PubMed
  192. Mainz RE, et al. 2022. Cells. 11:. PubMed
  193. Zhao F, et al. 2022. Front Immunol. 13:873720. PubMed
  194. Zhang P, et al. 2022. BMC Med. 20:435. PubMed
  195. Kang SJ, et al. 2022. Food Sci Anim Resour. 42:903. PubMed
  196. Carozza JA, et al. 2020. Nat Cancer. 184:1. PubMed
  197. Wang Y, et al. 2021. Sci Rep. 1.429861111. PubMed
  198. Reynolds A, et al. 2015. J Immunol. 194:231. PubMed
  199. Morris E, et al. 2005. J Clin Invest. 115:3093. PubMed
  200. Bertino P, et al. 2019. Oncoimmunology. 8:1601482. PubMed
  201. Tsukasaki M, et al. 2020. Cell Reports. 32(10):108124. PubMed
  202. Hernández–Santos N, et al. 2018. Cell Host Microbe. 1.313194444. PubMed
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RRID
AB_313320 (BioLegend Cat. No. 107605)
AB_313321 (BioLegend Cat. No. 107606)

Antigen Details

Structure
MHC class II
Distribution

B cell and activated T cells, APCs of the H-2b,d,q,r bearing mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14961 View all products for this Gene ID 14969 View all products for this Gene ID
UniProt
View information about I-A/I-E on UniProt.org

Other Formats

View All I-A/I-E Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse I-A/I-E (Maxpar® Ready) M5/114.15.2 FC,CyTOF®
Brilliant Violet 605™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 650™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 711™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 785™ anti-mouse I-A/I-E M5/114.15.2 FC
PE/Dazzle™ 594 anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 594 anti-mouse I-A/I-E M5/114.15.2 IHC-F,3D IHC
APC/Fire™ 750 anti-mouse I-A/I-E M5/114.15.2 FC
TotalSeq™-A0117 anti-mouse I-A/I-E M5/114.15.2 PG
Ultra-LEAF™ Purified anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,IP,Block
TotalSeq™-B0117 anti-mouse I-A/I-E M5/114.15.2 PG
TotalSeq™-C0117 anti-mouse I-A/I-E M5/114.15.2 PG
Spark Blue™ 550 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Fire™ 640 anti-mouse I-A/I-E M5/114.15.2 FC
Biotin anti-mouse I-A/I-E M5/114.15.2 FC
FITC anti-mouse I-A/I-E M5/114.15.2 FC
PE anti-mouse I-A/I-E M5/114.15.2 FC
Purified anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,IP,Block
PE/Cyanine5 anti-mouse I-A/I-E M5/114.15.2 FC
APC anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 488 anti-mouse I-A/I-E M5/114.15.2 FC,3D IHC
Alexa Fluor® 647 anti-mouse I-A/I-E M5/114.15.2 FC
Pacific Blue™ anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 700 anti-mouse I-A/I-E M5/114.15.2 FC,SB
PerCP/Cyanine5.5 anti-mouse I-A/I-E M5/114.15.2 FC
PerCP anti-mouse I-A/I-E M5/114.15.2 FC
APC/Cyanine7 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Cyanine7 anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 421™ anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,SB
Brilliant Violet 510™ anti-mouse I-A/I-E M5/114.15.2 FC
Spark YG™ 581 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Fire™ 810 anti-mouse I-A/I-E M5/114.15.2 FC
Spark UV™ 387 anti-mouse I-A/I-E M5/114.15.2 FC
Spark Violet™ 538 anti-mouse I-A/I-E M5/114.15.2 FC
PerCP/Fire™ 806 anti-mouse I-A/I-E M5/114.15.2 FC
APC/Fire™ 810 anti-mouse I-A/I-E M5/114.15.2 FC
Spark Red™ 718 anti-mouse I-A/I-E M5/114.15.2 FC
Spark PLUS UV395™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 750™ anti-mouse I-A/I-E M5/114.15.2 FC
Spark YG™ 593 anti-mouse I-A/I-E (Flexi-Fluor™) Antibody M5/114.15.2 FC
Spark NIR™ 685 anti-mouse I-A/I-E (Flexi-Fluor™) Antibody M5/114.15.2 FC
StarBright UltraViolet 795 anti-mouse I-A/I-E M5/114.15.2 FC
Spark PLUS B574™ anti-mouse I-A/I-E Antibody M5/114.15.2 FC
Go To Top Version: 4    Revision Date: 10/30/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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