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FITC anti-mouse I-A/I-E Antibody FITC anti-mouse I-A/I-E Antibody

Pricing & Availability
Clone
M5/114.15.2 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class II
Isotype
Rat IgG2b, κ
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Product Citations
publications
m5/114.15.2 FITC
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2) FITC (solid line) or rat IgG2b, κ FITC isotype control (dashed line).
  • m5/114.15.2 FITC
    C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2) FITC (solid line) or rat IgG2b, κ FITC isotype control (dashed line).
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Cat # Size Price Quantity Check Availability Save
107605 50 µg DKK422
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107606 500 µg DKK1307
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Description

These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Activated C57BL/6 mouse spleen cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens from mice carrying H-2p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-As MHC class II alloantigens.1

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6, in vitro and in vivo blocking of antigen presentation or ligand binding4-7, and spatial biology (IBEX)17,18. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).

Application References
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  18. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
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  41. Dutta S, et al. 2012. Eukaryot Cell. 0.756944444. PubMed
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  46. Sultan H, et al. 2018. Cancer Immunol Res. 6:617. PubMed
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  53. Wang H et al. 2017. Developmental cell. 40(6):566-582 . PubMed
  54. Kimura A, et al. 2018. J Exp Med. 215:2197. PubMed
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  56. Enriquez AB, et al. 2022. iScience. 25:104305. PubMed
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  58. Wang L, et al. 2021. Cancer Immunol Res. 9:348. PubMed
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  61. Morris E, et al. 2005. J Clin Invest. 115:3093. PubMed
  62. Bertino P, et al. 2019. Oncoimmunology. 8:1601482. PubMed
  63. Tsukasaki M, et al. 2020. Cell Reports. 32(10):108124. PubMed
  64. Carozza JA, et al. 2020. Nat Cancer. 184:1. PubMed
  65. Wang Y, et al. 2021. Sci Rep. 1.429861111. PubMed
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  68. Kang SJ, et al. 2022. Food Sci Anim Resour. 42:903. PubMed
  69. Reynolds A, et al. 2015. J Immunol. 194:231. PubMed
  70. Kaplan D, et al. 2007. J Exp Med. 204:2545. PubMed
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  72. Zou J, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:983. PubMed
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  75. Dobosz E, et al. 2021. Dis Model Mech. :14. PubMed
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  77. Koma T, et al. 2014. J Virol. 88:7178. PubMed
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  102. Luo M, et al. 2021. J Immunol. 207:1683. PubMed
  103. Hidaka T, et al. 2016. Nat Immunol. 18:64-73. PubMed
  104. Moufarrij S, et al. 2020. Sci Rep. 10:3470. PubMed
  105. Kelly SH, et al. 2021. Adv Healthc Mater. 10:e2001614. PubMed
  106. Bagati A, et al. 2020. Cancer Cell. 39(1):54-67.e9. PubMed
  107. Wu J, et al. 2019. J Immunol Res. 2019:1749803. PubMed
  108. Sivapatham S, et al. 2019. Front Immunol. 1.865277778. PubMed
  109. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  110. Xiao S, Manley N 2010. PLoS One. 5:e15396. PubMed
  111. Grube J, et al. 2022. Cell Death Dis. 13:704. PubMed
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  114. Schulz A, et al. 2015. J Biol Chem. 211: 553 - 567. PubMed
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  116. Nicholls J, et al. 2021. Front Cell Dev Biol. 9:737880. PubMed
  117. Chetty A, et al. 2021. Cell Host Microbe. 29:579. PubMed
  118. Aguilera TA, et al. 2020. Clin Cancer Res. 26:2972. PubMed
  119. Danne C et al. 2017. Cell host & microbe. 22(6):733-745 . PubMed
  120. Chryplewicz A, et al. 2022. Cancer Cell. 40:1111. PubMed
  121. Hong Y, et al. 2019. J Extracell Vesicles. 8:1670893. PubMed
  122. Krotova K, et al. 2019. Mol Ther Oncolytics. 15:166. PubMed
  123. Steinmann S, et al. 2020. Sci Rep. 1.160416667. PubMed
  124. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  125. Tran S, et al. 2020. Immunity. 53(3):627-640.e5. PubMed
  126. Al-Zaeed N, et al. 2021. Cell Death Dis. 12:611. PubMed
  127. Arima Y et al. 2017. eLife. 6 pii: e25517. PubMed
  128. Stack G, et al. 2015. PLoS Pathog. 11:1004641. PubMed
  129. Harberts E, et al. 2015. J Immunol. 194:3127. PubMed
  130. Park CJ, et al. 2020. Cell Reports. 31(2):107496.. PubMed
  131. Deets KA, et al. 2021. Elife. 10:. PubMed
  132. Spanier J, et al. 2016. Nat Commun. 7:11804. PubMed
  133. Dong S, et al. 2007. Int Immunopharmacol. 7:725. PubMed
  134. Nguyen T, et al. 2021. STAR Protoc. 2:101004. PubMed
  135. Wang S, et al. 2022. Sci Adv. 8:eabn3883. PubMed
  136. Gargaro M, et al. 2022. Immunity. 55:1032. PubMed
  137. Mugarza E, et al. 2022. Sci Adv. 8:eabm8780. PubMed
  138. Que W, et al. 2022. Sci Adv. 8:eabo4413. PubMed
  139. Montilla A, et al. 2023. Cell Death Dis. 14:16. PubMed
  140. Tanaka T, et al. 2023. Nat Immunol. 24:439. PubMed
  141. Drummer C, et al. 2023. Front Immunol. 14:1113883. PubMed
  142. Yang Z, et al. 2022. J Nucl Med. 63:1708. PubMed
  143. Lopez R, et al. 2022. Nat Biotechnol. 40:1360. PubMed
  144. Reticker-Flynn NE, et al. 2022. Cell. 185:1924. PubMed
  145. Sheng YH, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:789. PubMed
  146. Qiu Z, et al. 2022. J Exp Med. 219:. PubMed
  147. Sauter M, et al. 2022. STAR Protoc. 3:101664. PubMed
  148. Pagni RL, et al. 2022. Front Immunol. 13:1005937. PubMed
  149. Chen X, et al. 2023. Adv Mater. 35:e2210440. PubMed
  150. Zhao Q, et al. 2023. Signal Transduct Target Ther. 8:40. PubMed
  151. Do-Thi VA, et al. 2023. Cancer Res Commun. 3:80. PubMed
  152. Guo Z, et al. 2023. Int J Mol Sci. 24:. PubMed
  153. Yao H, et al. 2023. Front Immunol. 14:1147674. PubMed
  154. Shen Z, et al. 2023. iScience. 26:106572. PubMed
  155. He Y, et al. 2023. Biomolecules. 13:. PubMed
  156. Hsieh MS, et al. 2023. NPJ Vaccines. 8:82. PubMed
  157. Yao RQ, et al. 2023. Mil Med Res. 10:27. PubMed
  158. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  159. Sim SL, et al. 2022. Front Endocrinol (Lausanne). 13:957833. PubMed
  160. Tarban N, et al. 2022. Cells. 11:. PubMed
  161. Jong RM, et al. 2022. J Immunol. 208:407. PubMed
  162. Ma J, et al. 2020. Adv Sci (Weinh). 7:2000609. PubMed
  163. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  164. Pierini S, et al. 2021. J Immunother Cancer. 9:. PubMed
  165. Du J, et al. 2020. Developmental Cell. 55(6):737-753.e7. PubMed
  166. Orillion A, et al. 2018. Clin Cancer Res. 24:6383. PubMed
  167. Mulder R, et al. 2017. Front Immunol. 1.464583333. PubMed
  168. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  169. Sauter M, et al. 2022. iScience. 25:103677. PubMed
  170. Hickey A, et al. 2021. Front Microbiol. 12:653587. PubMed
  171. Misumi I et al. 2019. Cell Rep. 27(5):1387-1396 . PubMed
  172. Nakamura M, et al. 2015. J Immunol. 194:243. PubMed
  173. Aguilera T, et al. 2016. Nat Commun. 7:13898. PubMed
  174. Dutta S, et al. 2012. Eukaryot Cell. 0.756944444. PubMed
  175. He Y, et al. 2020. Sci Adv. 6:eaba7589. PubMed
  176. Hays C, et al. 2020. eLife. 8:e48772.. PubMed
  177. Matsumura F, et al. 2021. J Immunol. 207:938. PubMed
  178. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  179. Sultan H, et al. 2018. Cancer Immunol Res. 6:617. PubMed
  180. Zhao J, et al. 2019. Nat Commun. 10:899. PubMed
  181. Enriquez AB, et al. 2022. iScience. 25:104305. PubMed
  182. Renga G, et al. 2020. Life Sci Alliance. 3:. PubMed
  183. Zhu B, et al. 2021. Immunity. 54(6):1200-1218.e9. PubMed
  184. Xu X, et al. 2021. J Nanobiotechnology. 19:376. PubMed
  185. Ayasoufi K, et al. 2020. Brain. 3629:143. PubMed
  186. Wang H et al. 2017. Developmental cell. 40(6):566-582 . PubMed
  187. Kimura A, et al. 2018. J Exp Med. 215:2197. PubMed
  188. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  189. Enriquez AB, et al. 2022. iScience. 25:104305. PubMed
  190. Mainz RE, et al. 2022. Cells. 11:. PubMed
  191. Wang L, et al. 2021. Cancer Immunol Res. 9:348. PubMed
  192. Qu J, et al. 2018. J Immunol Res. 2018:7519856. PubMed
  193. Zhao F, et al. 2022. Front Immunol. 13:873720. PubMed
  194. Morris E, et al. 2005. J Clin Invest. 115:3093. PubMed
  195. Bertino P, et al. 2019. Oncoimmunology. 8:1601482. PubMed
  196. Tsukasaki M, et al. 2020. Cell Reports. 32(10):108124. PubMed
  197. Carozza JA, et al. 2020. Nat Cancer. 184:1. PubMed
  198. Wang Y, et al. 2021. Sci Rep. 1.429861111. PubMed
  199. Hernández–Santos N, et al. 2018. Cell Host Microbe. 1.313194444. PubMed
  200. Zhang P, et al. 2022. BMC Med. 20:435. PubMed
  201. Kang SJ, et al. 2022. Food Sci Anim Resour. 42:903. PubMed
  202. Reynolds A, et al. 2015. J Immunol. 194:231. PubMed
  203. Kaplan D, et al. 2007. J Exp Med. 204:2545. PubMed
  204. Alikhanyan K, et al. 2021. Cancers (Basel). 13:. PubMed
  205. Zou J, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:983. PubMed
  206. Miki Y, et al. 2016. J Biol Chem. 291: 15588 - 15601. PubMed
  207. Pradhan K, et al. 2021. Front Immunol. 12:778830. PubMed
  208. Dobosz E, et al. 2021. Dis Model Mech. :14. PubMed
  209. Bayik D, et al. 2020. Cancer Discov. 1.256944444. PubMed
  210. Koma T, et al. 2014. J Virol. 88:7178. PubMed
  211. Vorselen D, et al. 2021. Elife. 10:. PubMed
  212. Engler AE, et al. 2020. Cell Reports. 33(13):108553. PubMed
  213. Kim SH, et al. 2021. Cell Reports. 35(2):108995. PubMed
  214. Donaldson B, et al. 2017. J Immunother Cancer. 10.1186/s40425-017-0270-1. PubMed
  215. Zhang J, et al. 2018. Sci Rep. 8:2373. PubMed
  216. Hakim R, et al. 2019. Stem Cell Res Ther. 10:115. PubMed
  217. Kramer K, et al. 2017. ACS Omega. 0.240972222. PubMed
  218. White C, et al. 2015. J Immunol. 194:697. PubMed
  219. Otvos B, et al. 2021. Clin Cancer Res. 27:2038. PubMed
  220. Maulloo CD, et al. 2021. Front Immunol. 12:714842. PubMed
  221. Koikawa K, et al. 2021. Cell. 184:4753. PubMed
  222. Dewi IMW, et al. 2021. Cell Reports Medicine. 2(5):100289. PubMed
  223. Maisel D, et al. 2016. PLoS One. 11: 0159716. PubMed
  224. Steinbach K, et al. 2013. J Immunol. 191:4531. PubMed
  225. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  226. Guo S, et al. 2021. Sci Rep. 11:23745. PubMed
  227. Van Dis E, et al. 2018. Cell Rep. 23:1435. PubMed
  228. Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed
  229. Banovic T, et al. 2009. J Immunol. 182:912. PubMed
  230. Ghorbani S, et al. 2022. Nat Commun. 13:2445. PubMed
  231. Zhao L, et al. 2021. STAR Protocols. 2:100732. PubMed
  232. Zwick M, et al. 2019. Front Immunol. 10:222. PubMed
  233. Kang J, Lee J, Chang J 2016. PLoS One. 11: 0157015. PubMed
  234. Budai Z, et al. 2021. Cells. 10:. PubMed
  235. Luo M, et al. 2021. J Immunol. 207:1683. PubMed
  236. Hidaka T, et al. 2016. Nat Immunol. 18:64-73. PubMed
  237. Moufarrij S, et al. 2020. Sci Rep. 10:3470. PubMed
  238. Kelly SH, et al. 2021. Adv Healthc Mater. 10:e2001614. PubMed
  239. Bagati A, et al. 2020. Cancer Cell. 39(1):54-67.e9. PubMed
  240. Wu J, et al. 2019. J Immunol Res. 2019:1749803. PubMed
  241. Sivapatham S, et al. 2019. Front Immunol. 1.865277778. PubMed
  242. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  243. Xiao S, Manley N 2010. PLoS One. 5:e15396. PubMed
  244. Grube J, et al. 2022. Cell Death Dis. 13:704. PubMed
  245. Kramer K, et al. 2017. Mol Ther. 25(1):62-70. PubMed
  246. Wijayadikusumah A, et al. 2017. Amino Acids. 10.1007/s00726-017-2466-6. PubMed
  247. Schulz A, et al. 2015. J Biol Chem. 211: 553 - 567. PubMed
  248. Xiao Z, et al. 2022. Mater Today Bio. 15:100297. PubMed
  249. Nicholls J, et al. 2021. Front Cell Dev Biol. 9:737880. PubMed
  250. Chetty A, et al. 2021. Cell Host Microbe. 29:579. PubMed
  251. Aguilera TA, et al. 2020. Clin Cancer Res. 26:2972. PubMed
  252. Danne C et al. 2017. Cell host & microbe. 22(6):733-745 . PubMed
  253. Chryplewicz A, et al. 2022. Cancer Cell. 40:1111. PubMed
  254. Hong Y, et al. 2019. J Extracell Vesicles. 8:1670893. PubMed
  255. Krotova K, et al. 2019. Mol Ther Oncolytics. 15:166. PubMed
  256. Steinmann S, et al. 2020. Sci Rep. 1.160416667. PubMed
  257. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  258. Tran S, et al. 2020. Immunity. 53(3):627-640.e5. PubMed
  259. Al-Zaeed N, et al. 2021. Cell Death Dis. 12:611. PubMed
  260. Arima Y et al. 2017. eLife. 6 pii: e25517. PubMed
  261. Stack G, et al. 2015. PLoS Pathog. 11:1004641. PubMed
  262. Harberts E, et al. 2015. J Immunol. 194:3127. PubMed
  263. Park CJ, et al. 2020. Cell Reports. 31(2):107496.. PubMed
  264. Deets KA, et al. 2021. Elife. 10:. PubMed
  265. Spanier J, et al. 2016. Nat Commun. 7:11804. PubMed
  266. Dong S, et al. 2007. Int Immunopharmacol. 7:725. PubMed
RRID
AB_313320 (BioLegend Cat. No. 107605)
AB_313321 (BioLegend Cat. No. 107606)

Antigen Details

Structure
MHC class II
Distribution

B cell and activated T cells, APCs of the H-2b,d,q,r bearing mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14961 View all products for this Gene ID 14969 View all products for this Gene ID
UniProt
View information about I-A/I-E on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All I-A/I-E Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse I-A/I-E (Maxpar® Ready) M5/114.15.2 FC,CyTOF®
Brilliant Violet 605™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 650™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 711™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 785™ anti-mouse I-A/I-E M5/114.15.2 FC
PE/Dazzle™ 594 anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 594 anti-mouse I-A/I-E M5/114.15.2 IHC-F,3D IHC
APC/Fire™ 750 anti-mouse I-A/I-E M5/114.15.2 FC
TotalSeq™-A0117 anti-mouse I-A/I-E M5/114.15.2 PG
Ultra-LEAF™ Purified anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,IP,Block
TotalSeq™-B0117 anti-mouse I-A/I-E M5/114.15.2 PG
TotalSeq™-C0117 anti-mouse I-A/I-E M5/114.15.2 PG
Spark Blue™ 550 anti-mouse I-A/I-E M5/114.15.2 FC
Purified anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,IP,Block
PE/Fire™ 640 anti-mouse I-A/I-E M5/114.15.2 FC
Biotin anti-mouse I-A/I-E M5/114.15.2 FC
FITC anti-mouse I-A/I-E M5/114.15.2 FC
PE anti-mouse I-A/I-E M5/114.15.2 FC
PE/Cyanine5 anti-mouse I-A/I-E M5/114.15.2 FC
APC anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 488 anti-mouse I-A/I-E M5/114.15.2 FC,3D IHC
Alexa Fluor® 647 anti-mouse I-A/I-E M5/114.15.2 FC
Pacific Blue™ anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 700 anti-mouse I-A/I-E M5/114.15.2 FC,SB
PerCP anti-mouse I-A/I-E M5/114.15.2 FC
PerCP/Cyanine5.5 anti-mouse I-A/I-E M5/114.15.2 FC
APC/Cyanine7 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Cyanine7 anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 421™ anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,SB
Brilliant Violet 510™ anti-mouse I-A/I-E M5/114.15.2 FC
Spark YG™ 581 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Fire™ 810 anti-mouse I-A/I-E M5/114.15.2 FC
Spark UV™ 387 anti-mouse I-A/I-E M5/114.15.2 FC
Spark Violet™ 538 anti-mouse I-A/I-E M5/114.15.2 FC
PerCP/Fire™ 806 anti-mouse I-A/I-E M5/114.15.2 FC
Spark Red™ 718 anti-mouse I-A/I-E M5/114.15.2 FC
APC/Fire™ 810 anti-mouse I-A/I-E M5/114.15.2 FC
Spark PLUS UV395™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 750™ anti-mouse I-A/I-E M5/114.15.2 FC
Spark YG™ 593 anti-mouse I-A/I-E (Flexi-Fluor™) Antibody M5/114.15.2 FC
Spark NIR™ 685 anti-mouse I-A/I-E (Flexi-Fluor™) Antibody M5/114.15.2 FC
StarBright UltraViolet 795 anti-mouse I-A/I-E M5/114.15.2 FC
Spark PLUS B574™ anti-mouse I-A/I-E Antibody M5/114.15.2 FC
Go To Top Version: 4    Revision Date: 10/30/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Pricing & Availability
Clone
M5/114.15.2 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class II
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
m5/114.15.2 FITC
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2) FITC (solid line) or rat IgG2b, κ FITC isotype control (dashed line).
  • m5/114.15.2 FITC
    C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2) FITC (solid line) or rat IgG2b, κ FITC isotype control (dashed line).
Compare all formats See FITC spectral data
Cat # Size Price Quantity Check Availability Save
107605 50 µg DKK422
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107606 500 µg DKK1307
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Description

These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Activated C57BL/6 mouse spleen cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens from mice carrying H-2p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-As MHC class II alloantigens.1

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6, in vitro and in vivo blocking of antigen presentation or ligand binding4-7, and spatial biology (IBEX)17,18. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).

Application References
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  6. Guo M, et al. 1995. Zygote 3:65. (IHC)
  7. Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
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  16. Fu H, et al. 2014. Nat Commun. 5:3436. PubMed
  17. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  18. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
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  26. Sim SL, et al. 2022. Front Endocrinol (Lausanne). 13:957833. PubMed
  27. Tarban N, et al. 2022. Cells. 11:. PubMed
  28. Jong RM, et al. 2022. J Immunol. 208:407. PubMed
  29. Ma J, et al. 2020. Adv Sci (Weinh). 7:2000609. PubMed
  30. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
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  33. Orillion A, et al. 2018. Clin Cancer Res. 24:6383. PubMed
  34. Mulder R, et al. 2017. Front Immunol. 1.464583333. PubMed
  35. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  36. Sauter M, et al. 2022. iScience. 25:103677. PubMed
  37. Hickey A, et al. 2021. Front Microbiol. 12:653587. PubMed
  38. Misumi I et al. 2019. Cell Rep. 27(5):1387-1396 . PubMed
  39. Nakamura M, et al. 2015. J Immunol. 194:243. PubMed
  40. Aguilera T, et al. 2016. Nat Commun. 7:13898. PubMed
  41. Dutta S, et al. 2012. Eukaryot Cell. 0.756944444. PubMed
  42. He Y, et al. 2020. Sci Adv. 6:eaba7589. PubMed
  43. Hays C, et al. 2020. eLife. 8:e48772.. PubMed
  44. Matsumura F, et al. 2021. J Immunol. 207:938. PubMed
  45. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  46. Sultan H, et al. 2018. Cancer Immunol Res. 6:617. PubMed
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  48. Enriquez AB, et al. 2022. iScience. 25:104305. PubMed
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  50. Zhu B, et al. 2021. Immunity. 54(6):1200-1218.e9. PubMed
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  52. Ayasoufi K, et al. 2020. Brain. 3629:143. PubMed
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  137. Mugarza E, et al. 2022. Sci Adv. 8:eabm8780. PubMed
  138. Que W, et al. 2022. Sci Adv. 8:eabo4413. PubMed
  139. Montilla A, et al. 2023. Cell Death Dis. 14:16. PubMed
  140. Tanaka T, et al. 2023. Nat Immunol. 24:439. PubMed
  141. Drummer C, et al. 2023. Front Immunol. 14:1113883. PubMed
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  143. Lopez R, et al. 2022. Nat Biotechnol. 40:1360. PubMed
  144. Reticker-Flynn NE, et al. 2022. Cell. 185:1924. PubMed
  145. Sheng YH, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:789. PubMed
  146. Qiu Z, et al. 2022. J Exp Med. 219:. PubMed
  147. Sauter M, et al. 2022. STAR Protoc. 3:101664. PubMed
  148. Pagni RL, et al. 2022. Front Immunol. 13:1005937. PubMed
  149. Chen X, et al. 2023. Adv Mater. 35:e2210440. PubMed
  150. Zhao Q, et al. 2023. Signal Transduct Target Ther. 8:40. PubMed
  151. Do-Thi VA, et al. 2023. Cancer Res Commun. 3:80. PubMed
  152. Guo Z, et al. 2023. Int J Mol Sci. 24:. PubMed
  153. Yao H, et al. 2023. Front Immunol. 14:1147674. PubMed
  154. Shen Z, et al. 2023. iScience. 26:106572. PubMed
  155. He Y, et al. 2023. Biomolecules. 13:. PubMed
  156. Hsieh MS, et al. 2023. NPJ Vaccines. 8:82. PubMed
  157. Yao RQ, et al. 2023. Mil Med Res. 10:27. PubMed
  158. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  159. Sim SL, et al. 2022. Front Endocrinol (Lausanne). 13:957833. PubMed
  160. Tarban N, et al. 2022. Cells. 11:. PubMed
  161. Jong RM, et al. 2022. J Immunol. 208:407. PubMed
  162. Ma J, et al. 2020. Adv Sci (Weinh). 7:2000609. PubMed
  163. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  164. Pierini S, et al. 2021. J Immunother Cancer. 9:. PubMed
  165. Du J, et al. 2020. Developmental Cell. 55(6):737-753.e7. PubMed
  166. Orillion A, et al. 2018. Clin Cancer Res. 24:6383. PubMed
  167. Mulder R, et al. 2017. Front Immunol. 1.464583333. PubMed
  168. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  169. Sauter M, et al. 2022. iScience. 25:103677. PubMed
  170. Hickey A, et al. 2021. Front Microbiol. 12:653587. PubMed
  171. Misumi I et al. 2019. Cell Rep. 27(5):1387-1396 . PubMed
  172. Nakamura M, et al. 2015. J Immunol. 194:243. PubMed
  173. Aguilera T, et al. 2016. Nat Commun. 7:13898. PubMed
  174. Dutta S, et al. 2012. Eukaryot Cell. 0.756944444. PubMed
  175. He Y, et al. 2020. Sci Adv. 6:eaba7589. PubMed
  176. Hays C, et al. 2020. eLife. 8:e48772.. PubMed
  177. Matsumura F, et al. 2021. J Immunol. 207:938. PubMed
  178. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  179. Sultan H, et al. 2018. Cancer Immunol Res. 6:617. PubMed
  180. Zhao J, et al. 2019. Nat Commun. 10:899. PubMed
  181. Enriquez AB, et al. 2022. iScience. 25:104305. PubMed
  182. Renga G, et al. 2020. Life Sci Alliance. 3:. PubMed
  183. Zhu B, et al. 2021. Immunity. 54(6):1200-1218.e9. PubMed
  184. Xu X, et al. 2021. J Nanobiotechnology. 19:376. PubMed
  185. Ayasoufi K, et al. 2020. Brain. 3629:143. PubMed
  186. Wang H et al. 2017. Developmental cell. 40(6):566-582 . PubMed
  187. Kimura A, et al. 2018. J Exp Med. 215:2197. PubMed
  188. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  189. Enriquez AB, et al. 2022. iScience. 25:104305. PubMed
  190. Mainz RE, et al. 2022. Cells. 11:. PubMed
  191. Wang L, et al. 2021. Cancer Immunol Res. 9:348. PubMed
  192. Qu J, et al. 2018. J Immunol Res. 2018:7519856. PubMed
  193. Zhao F, et al. 2022. Front Immunol. 13:873720. PubMed
  194. Morris E, et al. 2005. J Clin Invest. 115:3093. PubMed
  195. Bertino P, et al. 2019. Oncoimmunology. 8:1601482. PubMed
  196. Tsukasaki M, et al. 2020. Cell Reports. 32(10):108124. PubMed
  197. Carozza JA, et al. 2020. Nat Cancer. 184:1. PubMed
  198. Wang Y, et al. 2021. Sci Rep. 1.429861111. PubMed
  199. Hernández–Santos N, et al. 2018. Cell Host Microbe. 1.313194444. PubMed
  200. Zhang P, et al. 2022. BMC Med. 20:435. PubMed
  201. Kang SJ, et al. 2022. Food Sci Anim Resour. 42:903. PubMed
  202. Reynolds A, et al. 2015. J Immunol. 194:231. PubMed
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RRID
AB_313320 (BioLegend Cat. No. 107605)
AB_313321 (BioLegend Cat. No. 107606)

Antigen Details

Structure
MHC class II
Distribution

B cell and activated T cells, APCs of the H-2b,d,q,r bearing mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14961 View all products for this Gene ID 14969 View all products for this Gene ID
UniProt
View information about I-A/I-E on UniProt.org

Other Formats

View All I-A/I-E Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse I-A/I-E (Maxpar® Ready) M5/114.15.2 FC,CyTOF®
Brilliant Violet 605™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 650™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 711™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 785™ anti-mouse I-A/I-E M5/114.15.2 FC
PE/Dazzle™ 594 anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 594 anti-mouse I-A/I-E M5/114.15.2 IHC-F,3D IHC
APC/Fire™ 750 anti-mouse I-A/I-E M5/114.15.2 FC
TotalSeq™-A0117 anti-mouse I-A/I-E M5/114.15.2 PG
Ultra-LEAF™ Purified anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,IP,Block
TotalSeq™-B0117 anti-mouse I-A/I-E M5/114.15.2 PG
TotalSeq™-C0117 anti-mouse I-A/I-E M5/114.15.2 PG
Spark Blue™ 550 anti-mouse I-A/I-E M5/114.15.2 FC
Purified anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,IP,Block
PE/Fire™ 640 anti-mouse I-A/I-E M5/114.15.2 FC
Biotin anti-mouse I-A/I-E M5/114.15.2 FC
FITC anti-mouse I-A/I-E M5/114.15.2 FC
PE anti-mouse I-A/I-E M5/114.15.2 FC
PE/Cyanine5 anti-mouse I-A/I-E M5/114.15.2 FC
APC anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 488 anti-mouse I-A/I-E M5/114.15.2 FC,3D IHC
Alexa Fluor® 647 anti-mouse I-A/I-E M5/114.15.2 FC
Pacific Blue™ anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 700 anti-mouse I-A/I-E M5/114.15.2 FC,SB
PerCP anti-mouse I-A/I-E M5/114.15.2 FC
PerCP/Cyanine5.5 anti-mouse I-A/I-E M5/114.15.2 FC
APC/Cyanine7 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Cyanine7 anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 421™ anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,SB
Brilliant Violet 510™ anti-mouse I-A/I-E M5/114.15.2 FC
Spark YG™ 581 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Fire™ 810 anti-mouse I-A/I-E M5/114.15.2 FC
Spark UV™ 387 anti-mouse I-A/I-E M5/114.15.2 FC
Spark Violet™ 538 anti-mouse I-A/I-E M5/114.15.2 FC
PerCP/Fire™ 806 anti-mouse I-A/I-E M5/114.15.2 FC
Spark Red™ 718 anti-mouse I-A/I-E M5/114.15.2 FC
APC/Fire™ 810 anti-mouse I-A/I-E M5/114.15.2 FC
Spark PLUS UV395™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 750™ anti-mouse I-A/I-E M5/114.15.2 FC
Spark YG™ 593 anti-mouse I-A/I-E (Flexi-Fluor™) Antibody M5/114.15.2 FC
Spark NIR™ 685 anti-mouse I-A/I-E (Flexi-Fluor™) Antibody M5/114.15.2 FC
StarBright UltraViolet 795 anti-mouse I-A/I-E M5/114.15.2 FC
Spark PLUS B574™ anti-mouse I-A/I-E Antibody M5/114.15.2 FC
Go To Top Version: 4    Revision Date: 10/30/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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