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- Regulatory Status
- RUO
- Other Names
- IL-6Ra (CD126), gp130 (CD130), TNF-RII (CD120b), IL-4Ra (CD124), PTX3, RAGE, TNF-RI (CD120a)
- Ave. Rating
- Submit a Review
- Product Citations
- publications
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 741665 | 100 tests | DKK6279 | ||||
Inflammation is a fundamental biological response that occurs when tissues are injured, infected, or exposed to harmful stimuli, and involves various soluble receptors, growth factors, cytokines, and chemokines. Accurate measurement of these key mediators is crucial for inflammation research related to disease progression and immune responses. The LEGENDplex™ Mouse Inflammation Panel 2 (7-plex) is a bead-based multiplex assay panel, using fluorescence-encoded beads suitable for use on various flow cytometers. It allows for simultaneous quantification of 7 mouse proteins related to inflammation, including IL-6Ra (CD126), gp130 (CD130), TNF-RII (CD120b), IL-4Ra (CD124), PTX3, RAGE, TNF-RI (CD120a). This panel provides higher sensitivity and broader dynamic range than traditional ELISA methods. The panel has been validated for use on serum, plasma and cell culture supernatant sample. The LEGENDplex™ Mouse Inflammation Panel 2 (7-plex) is designed to allow flexible customization within the panel. It can also be divided into subpanels.
Product DetailsKit Details
- Kit Contents
-
- Setup Beads: PE Beads
- Setup Beads: Raw Beads
- LEGENDplex™ Mouse Inflammation Panel 2 Capture Beads
- LEGENDplex™ Mouse Inflammation Panel 2 Detection Abs
- LEGENDplex™ Mouse Inflammation Panel 2 Standard
- LEGENDplex™ Lyophilized Standard Reconstitution Buffer
- LEGENDplex™ Matrix C, Lyophilized
- LEGENDplex™ SA-PE
- LEGENDplex™ Assay Buffer
- LEGENDplex™ Wash Buffer, 20X
- Filter Plate
- Plate Sealers
- Materials Not Included
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- Flow Cytometer
- Pipettes and Tips
- Reagent Reservoirs for Multichannel Pipettes
- Polypropylene Microfuge Tubes
- Vortex Mixer
- Sonicator
- Aluminum Foil
- Absorbent Pads or Paper Towels
- Plate Shaker
- Tabletop CentrifugesA Vacuum Filtration Unit and a Vacuum Source (if using filter plates)
- 1.1 mL polypropylene micro FACS tubes, in 96-tube rack
- Manual
Product Details
- Verified Reactivity
- Mouse
- Application
-
Multiplex
Learn more about LEGENDplex™ at biolegend.com/legendplex
Download the LEGENDplex™ software here.
Antigen Details
- Cell Type
- Antigen-presenting cells, B cells, Endothelial cells, Fibroblasts, Leukocytes, Lymphocytes, Macrophages, T cells, Tregs
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Costimulatory Molecules, Immuno-Oncology, Immunology, Inhibitory Molecules, Innate Immunity, Signal Transduction
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors, Soluble Receptors
- Gene ID
- 16194 View all products for this Gene ID 16195 View all products for this Gene ID 21938 View all products for this Gene ID 16190 View all products for this Gene ID 19288 View all products for this Gene ID 11596 View all products for this Gene ID 21937 View all products for this Gene ID
Related FAQs
- If I don't have a vacuum, how do I remove the liquid from my plate?
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If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.
- Should I perform the assay with the filter plates or with V-bottom plates?
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Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
- After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
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The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
- What is the shelf life of LEGENDplex™ kits?
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LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.
- Is special software required for data analysis?
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Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.
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