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ELISA MAX™ Deluxe Set Mouse TNF-α ELISA MAX™ Deluxe Set Mouse TNF-α

Pricing & Availability
Regulatory Status
RUO
Other Names
TNF-a ELISA Kit
Ave. Rating
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Product Citations
publications
mouse tnf-a_122109
  • mouse tnf-a_122109
Cat # Size Price Quantity Check Availability Save
430904 5 plates DKK1812
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430915 10 Plates DKK3481
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430916 20 Plates DKK5572
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Description

ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend's ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price!

BioLegend's ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Product Details
Technical Data Sheet (pdf)

Kit Details

Kit Contents
  • Mouse TNF-α ELISA MAX™ Capture Antibody (200X)
  • Mouse TNF-α ELISA MAX™ Detection Antibody (200X)
  • Mouse TNF-α Standard
  • Avidin-HRP (1000X)
  • Substrate Solution A
  • Substrate Solution B
  • Coating Buffer A (5X)
  • Assay Diluent A (5X)
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered.
  • Wash Buffer (Phosphate-Buffered Saline (PBS) + 0.05% Tween-20, pH=7.4). BioLegend Cat. No. 421601 is recommended.
  • Wash bottle or automated microplate washer
  • Stop Solution (e.g. 2N H2SO4) Biolegend Cat. No. 423001 is recommended
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Sealer
  • Absorbent paper

Product Details

Verified Reactivity
Mouse
Application

ELISA
 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Application References
  1. Patole PS, et al. 2005. J. Am. Soc. Nephrol. 16:3273.
  2. ChavantF, et al. 2010. J. Pharmacol Exp. Ther. 332:505. PubMed
  3. Lewis CJ, et al. 2010. J. Immunol. 184:3789. PubMed
  4. Qui J, et al. Appl Environ Microbiol. PubMed
  5. Yang HJ, et al. 2011. J Cell Sci. 124:2552. PubMed.
  6. White LE, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 303:449. PubMed
  7. Zhang X, et al. 2014. PLoS One. 9:90856. PubMed
  8. Zachman AL, et al. 2014. Biomaterials. 35:9635. PubMed
  9. Goldberg R, et al. 2014. Diabetes. 63:4302. PubMed
  10. Zahman AL, et al. 2014. Biomaterials. 35:9635. PubMed
  11. Justice JN, et al. 2015. J appl Physiol. 118:163. PubMed
Product Citations
  1. Ahangar P, et al. 2021. Wound Repair Regen. 29:380. PubMed
  2. Wang JJ, et al. 2022. J Biol Chem. 298:102017. PubMed
  3. Sugimoto S, et al. 2022. Nat Metab. 4:775. PubMed
  4. Yu M, et al. 2022. Front Pharmacol. 13:985618. PubMed
  5. Cheng P, et al. 2023. Pharmacol Res. 188:106643. PubMed
  6. Zhao C, et al. 2023. PLoS Pathog. 19:e1011108. PubMed
  7. Wei K, et al. 2023. Signal Transduct Target Ther. 8:22. PubMed
  8. Sun XY, et al. 2023. Signal Transduct Target Ther. 8:30. PubMed
  9. Zhao C, et al. 2023. NPJ Biofilms Microbiomes. 9:8. PubMed
  10. Hsu CG, et al. 2022. Cell Death Differ. 29:1790. PubMed
  11. Tsai YR, et al. 2022. Pharmaceutics. 14:. PubMed
  12. Cho MJ, et al. 2023. Exp Mol Med. 55:1033. PubMed
  13. Foster SL, et al. 2023. Cells. 12:. PubMed
  14. Amirova KM, et al. 2023. Int J Mol Sci. 24:. PubMed
  15. Gao J, et al. 2023. Pharmaceutics. 15:. PubMed
  16. Dong X, et al. 2019. ACS Nano. 13:1272. PubMed
  17. Guo W, et al. 2021. Int J Biol Sci. 17:4271. PubMed
  18. Chou WC, et al. 2021. Nature. 591:300. PubMed
  19. Ishikawa S, et al. 2021. Biol Pharm Bull. 44:550. PubMed
  20. Cerrato G, et al. 2021. Cell Death Differ. 28:1733. PubMed
  21. Luo W, et al. 2018. Bioorg Med Chem. 26:1547. PubMed
  22. Radwanska M, et al. 2021. Vaccines (Basel). 9:. PubMed
  23. Shih S, et al. 2016. PLoS One. 11: 0157647. PubMed
  24. Kim MW, et al. 2020. Front Bioeng Biotechnol. 8:133. PubMed
  25. Chen M, et al. 2022. J Nanobiotechnology. 20:273. PubMed
  26. Li DY, et al. 2018. Sci Rep. 8:17979. PubMed
  27. Nagata K, et al. 2020. Int Immunol. 32:143. PubMed
  28. Yang Y, et al. 2020. J Int Med Res. 48:300060520902605. PubMed
  29. Ma X, et al. 2022. Cell Mol Immunol. . PubMed
  30. Kalafatakis I, et al. 2021. J Neurosci Res. 99:1474. PubMed
  31. Du Q, et al. 2019. Front Microbiol. 10:2050. PubMed
  32. Zachman A, et al. 2014. Biomaterials. 35:9635. PubMed
  33. Yang W, et al. 2020. Nat Commun. 3.553472222. PubMed
  34. Naji A, et al. 2016. Sci Rep. 6: 26162. PubMed
  35. Sun T, et al. 2018. Front Immunol. 9:1245. PubMed
  36. Li H, et al. 2021. Nat Commun. 12:2773. PubMed
  37. Li H, et al. 2021. Mol Med Rep. 1:23. PubMed
  38. Clausen B, et al. 2016. Sci Rep. 6:29291. PubMed
  39. Zhang B, et al. 2021. Exp Ther Med. 22:832. PubMed
  40. Lv ZY, et al. 2022. J Inflamm Res. 15:2819. PubMed
  41. Kohn M, et al. 2021. Front Immunol. 580594:12. PubMed
  42. Ye H, et al. 2016. Exp Mol Pathol. 100: 307-311. PubMed
  43. Liu X, et al. 2021. Int J Mol Sci. 22:. PubMed
  44. Ahangar P, et al. 2020. NPJ Regen Med. 24:5. PubMed
  45. Brunetti J, et al. 2016. J Biol Chem. 291(49):25742-25748. PubMed
  46. Yang H, et al. 2011. J Cell Sci. 124:2552. PubMed
  47. Park HB, et al. 2020. Oncoimmunology. 9:1772663. PubMed
  48. Shaker ME, et al. 2022. Saudi Pharm J. 30:291. PubMed
  49. Schaafsma W, et al. 2017. Neurobiology of Disease. 10.1016/j.nbd.2017.07.017. PubMed
  50. Park GT, et al. 2021. Mar Drugs. 19:. PubMed
  51. Lehmann M, et al. 2016. J Leukoc Biol. 99: 1057 - 1064. PubMed
  52. Lin CC, et al. 2021. Iran J Basic Med Sci. 24:1146. PubMed
  53. Li Y, et al. 2021. J Neurochem. 159:867. PubMed
  54. Chen G, et al. 2021. Sci Adv. 7:eabg5686. PubMed
  55. Li Y, et al. 2021. Sci Transl Med. 13:. PubMed
  56. Zhang X, et al. 2014. PLoS One. 9:90856. PubMed
  57. Qiu J, et al. 2010. Appl Envir Microbiol. 76:5846. PubMed
  58. Sousa AH, et al. 2021. Can J Physiol Pharmacol. 99:1324. PubMed
  59. Hsu CY, et al. 2022. Int J Mol Sci. 23:. PubMed
  60. Dai X, et al. 2017. FASEB J. 31:2881. PubMed
  61. Liu R, et al. 2018. Exp Ther Med. 15:3385. PubMed
  62. Iannucci J, et al. 2020. Cell Mol Neurobiol. . PubMed
  63. Hsueh SC, et al. 2022. Biomedicines. 10:. PubMed
  64. Yu Y, et al. 2021. Cell Rep. 37:110126. PubMed
  65. Wang J, et al. 2022. J Clin Invest. :. PubMed
  66. Lee N, et al. 2022. Front Immunol. 13:940258. PubMed
  67. Liu TH, et al. 2018. Nutrients. 10:E472. PubMed
  68. Medrano–Jiménez E, et al. 2019. J Neuroinflammation. 16:143. PubMed
  69. Park GT, et al. 2019. Front Immunol. 10:2095. PubMed
  70. Fan Q, et al. 2021. Bioact Mater. 6:4014. PubMed
  71. Al Mamun A, et al. 2020. Proc Natl Acad Sci U S A. 117:1742. PubMed
  72. Ci T, et al. 2020. Sci Adv. 6:00. PubMed
  73. Wilson JL, et al. 2020. Cell Rep. 30:1542. PubMed
  74. Qi S, et al. 2022. Transl Stroke Res. :. PubMed
  75. Hsu CG, et al. 2021. J Immunol. 206:3010. PubMed
  76. Xiahou Z,et al. 2017. Nat Commun. . 10.1038/s41467-017-00930-9. PubMed
  77. Zeng Q, et al. 2022. iScience. 25:105151. PubMed
  78. Sun B, et al. 2018. Cell Mol Immunol. 1.168055556. PubMed
  79. Li WS, et al. 2021. Int J Mol Sci. 22:. PubMed
  80. Nuro-Gyina PK, et al. 2021. J Immunol. 207:868. PubMed
  81. Zhou XM, et al. 2018. Front Pharmacol. 8:904. PubMed
  82. Wang C, et al. 2020. Redox Biol. 37:101688. PubMed
  83. White L, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 303:449. PubMed
  84. Wang Y, et al. 2021. Nat Commun. 12:4964. PubMed
  85. Stanfield BA, et al. 2021. PLoS One. 16:e0245169. PubMed
  86. Justice J, et al. 2015. J Appl Physiol. 118:163. PubMed
  87. Chan K, et al. 2015. J Biol Chem. 290: 16979 - 16988. PubMed
  88. Scanu A, et al. 2022. Int J Mol Sci. 23:. PubMed
  89. Deng LR, et al. 2022. Chin Med. 17:132. PubMed
  90. Lanfermann C, et al. 2021. Vaccines (Basel). 9:. PubMed
  91. Feng L, et al. 2022. Signal Transduct Target Ther. 7:21. PubMed
  92. Yang FM, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  93. Pan Y, et al. 2021. Br J Pharmacol. 178:4907. PubMed
  94. Gu H, et al. 2021. Elife. 10:. PubMed
  95. Zhuo X, et al. 2021. Biomed Rep. 15:64. PubMed
  96. Schaafsma W, et al. 2015. Brain Behav Immun. 48: 205-221. PubMed
  97. Nagata K, et al. 2021. Front Immunol. 12:730706. PubMed
  98. Lanfermann C, et al. 2021. Vaccines (Basel). 9:. PubMed
  99. de León-Guerrero SD, et al. 2022. Dis Model Mech. . PubMed
  100. Su S, et al. 2022. Signal Transduct Target Ther. 7:137. PubMed
  101. Agrahari G, et al. 2021. Front Immunol. 12:628117. PubMed
  102. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
  103. Goldberg R, et al. 2014. Diabetes. 63:4302. PubMed
  104. Li X, et al. 2022. Sci Rep. 12:17058. PubMed
  105. Cobb C 2010. J Immunol. 184:3789. PubMed
  106. Chavant F, et al. 2010. J Pharmacol Exp Ther. 332:505. PubMed
  107. Reed MD, et al. 2020. Nature. 577:249. PubMed
  108. Ratna A, et al. 2021. Hepatol Commun. 5:1165. PubMed
  109. Fante T, et al. 2016. PLoS One. 11: 0160184. PubMed
  110. Chen Y, et al. 2018. Exp Ther Med. 16:4322. PubMed
  111. Ahangar P, et al. 2021. Wound Repair Regen. 29:380. PubMed
  112. Wang JJ, et al. 2022. J Biol Chem. 298:102017. PubMed
  113. Sugimoto S, et al. 2022. Nat Metab. 4:775. PubMed
  114. Yu M, et al. 2022. Front Pharmacol. 13:985618. PubMed
  115. Cheng P, et al. 2023. Pharmacol Res. 188:106643. PubMed
  116. Zhao C, et al. 2023. PLoS Pathog. 19:e1011108. PubMed
  117. Wei K, et al. 2023. Signal Transduct Target Ther. 8:22. PubMed
  118. Sun XY, et al. 2023. Signal Transduct Target Ther. 8:30. PubMed
  119. Zhao C, et al. 2023. NPJ Biofilms Microbiomes. 9:8. PubMed
  120. Hsu CG, et al. 2022. Cell Death Differ. 29:1790. PubMed
  121. Tsai YR, et al. 2022. Pharmaceutics. 14:. PubMed
  122. Cho MJ, et al. 2023. Exp Mol Med. 55:1033. PubMed
  123. Foster SL, et al. 2023. Cells. 12:. PubMed
  124. Amirova KM, et al. 2023. Int J Mol Sci. 24:. PubMed
  125. Gao J, et al. 2023. Pharmaceutics. 15:. PubMed
  126. Dong X, et al. 2019. ACS Nano. 13:1272. PubMed
  127. Guo W, et al. 2021. Int J Biol Sci. 17:4271. PubMed
  128. Chou WC, et al. 2021. Nature. 591:300. PubMed
  129. Ishikawa S, et al. 2021. Biol Pharm Bull. 44:550. PubMed
  130. Cerrato G, et al. 2021. Cell Death Differ. 28:1733. PubMed
  131. Luo W, et al. 2018. Bioorg Med Chem. 26:1547. PubMed
  132. Radwanska M, et al. 2021. Vaccines (Basel). 9:. PubMed
  133. Shih S, et al. 2016. PLoS One. 11: 0157647. PubMed
  134. Kim MW, et al. 2020. Front Bioeng Biotechnol. 8:133. PubMed
  135. Chen M, et al. 2022. J Nanobiotechnology. 20:273. PubMed
  136. Li DY, et al. 2018. Sci Rep. 8:17979. PubMed
  137. Nagata K, et al. 2020. Int Immunol. 32:143. PubMed
  138. Yang Y, et al. 2020. J Int Med Res. 48:300060520902605. PubMed
  139. Ma X, et al. 2022. Cell Mol Immunol. . PubMed
  140. Kalafatakis I, et al. 2021. J Neurosci Res. 99:1474. PubMed
  141. Du Q, et al. 2019. Front Microbiol. 10:2050. PubMed
  142. Zachman A, et al. 2014. Biomaterials. 35:9635. PubMed
  143. Yang W, et al. 2020. Nat Commun. 3.553472222. PubMed
  144. Naji A, et al. 2016. Sci Rep. 6: 26162. PubMed
  145. Sun T, et al. 2018. Front Immunol. 9:1245. PubMed
  146. Li H, et al. 2021. Nat Commun. 12:2773. PubMed
  147. Li H, et al. 2021. Mol Med Rep. 1:23. PubMed
  148. Clausen B, et al. 2016. Sci Rep. 6:29291. PubMed
  149. Zhang B, et al. 2021. Exp Ther Med. 22:832. PubMed
  150. Lv ZY, et al. 2022. J Inflamm Res. 15:2819. PubMed
  151. Kohn M, et al. 2021. Front Immunol. 580594:12. PubMed
  152. Ye H, et al. 2016. Exp Mol Pathol. 100: 307-311. PubMed
  153. Liu X, et al. 2021. Int J Mol Sci. 22:. PubMed
  154. Ahangar P, et al. 2020. NPJ Regen Med. 24:5. PubMed
  155. Brunetti J, et al. 2016. J Biol Chem. 291(49):25742-25748. PubMed
  156. Yang H, et al. 2011. J Cell Sci. 124:2552. PubMed
  157. Park HB, et al. 2020. Oncoimmunology. 9:1772663. PubMed
  158. Shaker ME, et al. 2022. Saudi Pharm J. 30:291. PubMed
  159. Schaafsma W, et al. 2017. Neurobiology of Disease. 10.1016/j.nbd.2017.07.017. PubMed
  160. Park GT, et al. 2021. Mar Drugs. 19:. PubMed
  161. Lehmann M, et al. 2016. J Leukoc Biol. 99: 1057 - 1064. PubMed
  162. Lin CC, et al. 2021. Iran J Basic Med Sci. 24:1146. PubMed
  163. Li Y, et al. 2021. J Neurochem. 159:867. PubMed
  164. Chen G, et al. 2021. Sci Adv. 7:eabg5686. PubMed
  165. Li Y, et al. 2021. Sci Transl Med. 13:. PubMed
  166. Zhang X, et al. 2014. PLoS One. 9:90856. PubMed
  167. Qiu J, et al. 2010. Appl Envir Microbiol. 76:5846. PubMed
  168. Sousa AH, et al. 2021. Can J Physiol Pharmacol. 99:1324. PubMed
  169. Hsu CY, et al. 2022. Int J Mol Sci. 23:. PubMed
  170. Dai X, et al. 2017. FASEB J. 31:2881. PubMed
  171. Liu R, et al. 2018. Exp Ther Med. 15:3385. PubMed
  172. Iannucci J, et al. 2020. Cell Mol Neurobiol. . PubMed
  173. Hsueh SC, et al. 2022. Biomedicines. 10:. PubMed
  174. Yu Y, et al. 2021. Cell Rep. 37:110126. PubMed
  175. Wang J, et al. 2022. J Clin Invest. :. PubMed
  176. Lee N, et al. 2022. Front Immunol. 13:940258. PubMed
  177. Liu TH, et al. 2018. Nutrients. 10:E472. PubMed
  178. Medrano–Jiménez E, et al. 2019. J Neuroinflammation. 16:143. PubMed
  179. Park GT, et al. 2019. Front Immunol. 10:2095. PubMed
  180. Fan Q, et al. 2021. Bioact Mater. 6:4014. PubMed
  181. Al Mamun A, et al. 2020. Proc Natl Acad Sci U S A. 117:1742. PubMed
  182. Ci T, et al. 2020. Sci Adv. 6:00. PubMed
  183. Wilson JL, et al. 2020. Cell Rep. 30:1542. PubMed
  184. Qi S, et al. 2022. Transl Stroke Res. :. PubMed
  185. Hsu CG, et al. 2021. J Immunol. 206:3010. PubMed
  186. Xiahou Z,et al. 2017. Nat Commun. . 10.1038/s41467-017-00930-9. PubMed
  187. Zeng Q, et al. 2022. iScience. 25:105151. PubMed
  188. Sun B, et al. 2018. Cell Mol Immunol. 1.168055556. PubMed
  189. Li WS, et al. 2021. Int J Mol Sci. 22:. PubMed
  190. Nuro-Gyina PK, et al. 2021. J Immunol. 207:868. PubMed
  191. Zhou XM, et al. 2018. Front Pharmacol. 8:904. PubMed
  192. Wang C, et al. 2020. Redox Biol. 37:101688. PubMed
  193. White L, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 303:449. PubMed
  194. Wang Y, et al. 2021. Nat Commun. 12:4964. PubMed
  195. Stanfield BA, et al. 2021. PLoS One. 16:e0245169. PubMed
  196. Justice J, et al. 2015. J Appl Physiol. 118:163. PubMed
  197. Chan K, et al. 2015. J Biol Chem. 290: 16979 - 16988. PubMed
  198. Scanu A, et al. 2022. Int J Mol Sci. 23:. PubMed
  199. Deng LR, et al. 2022. Chin Med. 17:132. PubMed
  200. Lanfermann C, et al. 2021. Vaccines (Basel). 9:. PubMed
  201. Feng L, et al. 2022. Signal Transduct Target Ther. 7:21. PubMed
  202. Yang FM, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  203. Pan Y, et al. 2021. Br J Pharmacol. 178:4907. PubMed
  204. Gu H, et al. 2021. Elife. 10:. PubMed
  205. Zhuo X, et al. 2021. Biomed Rep. 15:64. PubMed
  206. Schaafsma W, et al. 2015. Brain Behav Immun. 48: 205-221. PubMed
  207. Nagata K, et al. 2021. Front Immunol. 12:730706. PubMed
  208. Lanfermann C, et al. 2021. Vaccines (Basel). 9:. PubMed
  209. de León-Guerrero SD, et al. 2022. Dis Model Mech. . PubMed
  210. Su S, et al. 2022. Signal Transduct Target Ther. 7:137. PubMed
  211. Agrahari G, et al. 2021. Front Immunol. 12:628117. PubMed
  212. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
  213. Goldberg R, et al. 2014. Diabetes. 63:4302. PubMed
  214. Li X, et al. 2022. Sci Rep. 12:17058. PubMed
  215. Cobb C 2010. J Immunol. 184:3789. PubMed
  216. Chavant F, et al. 2010. J Pharmacol Exp Ther. 332:505. PubMed
  217. Reed MD, et al. 2020. Nature. 577:249. PubMed
  218. Ratna A, et al. 2021. Hepatol Commun. 5:1165. PubMed
  219. Fante T, et al. 2016. PLoS One. 11: 0160184. PubMed
  220. Chen Y, et al. 2018. Exp Ther Med. 16:4322. PubMed
Sensitivity
4 pg/mL
Standard Range
7.8-500 pg/mL

Antigen Details

Cell Sources
Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Gene ID
21926 View all products for this Gene ID
UniProt
View information about TNF-alpha on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 4    Revision Date: 12/14/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

Pricing & Availability
Regulatory Status
RUO
Other Names
TNF-a ELISA Kit
Ave. Rating
Submit a Review
Product Citations
publications
mouse tnf-a_122109
  • mouse tnf-a_122109
Cat # Size Price Quantity Check Availability Save
430904 5 plates DKK1812
Check Availability


Need larger quantities of this item?
Request Bulk Quote
430915 10 Plates DKK3481
Check Availability


Need larger quantities of this item?
Request Bulk Quote
430916 20 Plates DKK5572
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend's ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price!

BioLegend's ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Product Details
Technical Data Sheet (pdf)

Kit Details

Kit Contents
  • Mouse TNF-α ELISA MAX™ Capture Antibody (200X)
  • Mouse TNF-α ELISA MAX™ Detection Antibody (200X)
  • Mouse TNF-α Standard
  • Avidin-HRP (1000X)
  • Substrate Solution A
  • Substrate Solution B
  • Coating Buffer A (5X)
  • Assay Diluent A (5X)
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered.
  • Wash Buffer (Phosphate-Buffered Saline (PBS) + 0.05% Tween-20, pH=7.4). BioLegend Cat. No. 421601 is recommended.
  • Wash bottle or automated microplate washer
  • Stop Solution (e.g. 2N H2SO4) Biolegend Cat. No. 423001 is recommended
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Sealer
  • Absorbent paper

Product Details

Verified Reactivity
Mouse
Application

ELISA
 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Application References
  1. Patole PS, et al. 2005. J. Am. Soc. Nephrol. 16:3273.
  2. ChavantF, et al. 2010. J. Pharmacol Exp. Ther. 332:505. PubMed
  3. Lewis CJ, et al. 2010. J. Immunol. 184:3789. PubMed
  4. Qui J, et al. Appl Environ Microbiol. PubMed
  5. Yang HJ, et al. 2011. J Cell Sci. 124:2552. PubMed.
  6. White LE, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 303:449. PubMed
  7. Zhang X, et al. 2014. PLoS One. 9:90856. PubMed
  8. Zachman AL, et al. 2014. Biomaterials. 35:9635. PubMed
  9. Goldberg R, et al. 2014. Diabetes. 63:4302. PubMed
  10. Zahman AL, et al. 2014. Biomaterials. 35:9635. PubMed
  11. Justice JN, et al. 2015. J appl Physiol. 118:163. PubMed
Product Citations
  1. Ahangar P, et al. 2021. Wound Repair Regen. 29:380. PubMed
  2. Wang JJ, et al. 2022. J Biol Chem. 298:102017. PubMed
  3. Sugimoto S, et al. 2022. Nat Metab. 4:775. PubMed
  4. Yu M, et al. 2022. Front Pharmacol. 13:985618. PubMed
  5. Cheng P, et al. 2023. Pharmacol Res. 188:106643. PubMed
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  216. Chavant F, et al. 2010. J Pharmacol Exp Ther. 332:505. PubMed
  217. Reed MD, et al. 2020. Nature. 577:249. PubMed
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Sensitivity
4 pg/mL
Standard Range
7.8-500 pg/mL

Antigen Details

Cell Sources
Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Gene ID
21926 View all products for this Gene ID
UniProt
View information about TNF-alpha on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 4    Revision Date: 12/14/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

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