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PE anti-mouse CD4 Antibody PE anti-mouse CD4 Antibody

Pricing & Availability
Clone
H129.19 (See other available formats)
Regulatory Status
RUO
Other Names
L3T4, T4
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
H129dot19_PE_011909.jpg
C57BL/6 splenocytes stained with H129.19 PE
  • H129dot19_PE_011909.jpg
    C57BL/6 splenocytes stained with H129.19 PE
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
130310 200 µg DKK1010
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description CD4 is a 55 kD protein, also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosin kinase, lck. The H129.19 antibody is able to block CD4 mediated cell adhesion and T cell activation. It is also useful for in vivo and in vitro depletion of CD4+ T cells. H129.19 antibody blocks binding of the anti-mouse CD4 mAbs GK1.5 and RM4-5, but not RM4-4 antibody. H129.19 antibody inhibits various responses of T helper cells to antigenic or mitogenic stimuli. Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse lymphocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: blocking of CD4+ T cell activation1-3, depletion. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays.

Application References
  1. Naquet P. 1985. Immunogenetics. 22:247-256.
  2. Pierres A. 1984. J. Immunol.. 132:2775-2782.
  3. Guo MW. 1995. Zygote. 3:65-73.
  4. Ghobrial RR. 1989. Clin Immunol Immunopathol. 52:486-506.
Product Citations
  1. Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
  2. Chen Y, et al. 2023. Adv Healthc Mater. 12:e2202085. PubMed
  3. Horkova V, et al. 2023. Nat Immunol. 24:174. PubMed
  4. Wang D, et al. 2023. Nat Commun. 14:2943. PubMed
  5. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
  6. Sá da Bandeira D, et al. 2022. Cell Rep. 40:111114. PubMed
  7. Zhang C, et al. 2022. Nat Commun. 13:3468. PubMed
  8. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  9. Oka Y, et al. 2020. Sci Adv. 6:. PubMed
  10. Jiang Y, et al. 2021. Nat Commun. 12:742. PubMed
  11. Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
  12. Chen Y, et al. 2023. Adv Healthc Mater. 12:e2202085. PubMed
  13. Horkova V, et al. 2023. Nat Immunol. 24:174. PubMed
  14. Wang D, et al. 2023. Nat Commun. 14:2943. PubMed
  15. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
  16. Sá da Bandeira D, et al. 2022. Cell Rep. 40:111114. PubMed
  17. Zhang C, et al. 2022. Nat Commun. 13:3468. PubMed
  18. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  19. Oka Y, et al. 2020. Sci Adv. 6:. PubMed
  20. Jiang Y, et al. 2021. Nat Commun. 12:742. PubMed
RRID
AB_2075573 (BioLegend Cat. No. 130310)

Antigen Details

Structure
Ig superfamily, 55 kD
Distribution
Majority of thymocytes, T cell subset
Function
TCR co-receptor, T cell activation
Ligand/Receptor
MHC class II molecule
Cell Type
Thymocytes, T cells, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.
4. Naquet P. 1985. Immunogenetics. 22:247-256.
5. Pierres A. 1984. J. Immunol.. 132:2775-2782.
6. Guo MW. 1995. Zygote. 3:65-73.
7. Ghobrial RR. 1989. Clin Immunol Immunopathol. 52:486-506.
Gene ID
12504 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Pricing & Availability
Clone
H129.19 (See other available formats)
Regulatory Status
RUO
Other Names
L3T4, T4
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
H129dot19_PE_011909.jpg
C57BL/6 splenocytes stained with H129.19 PE
  • H129dot19_PE_011909.jpg
    C57BL/6 splenocytes stained with H129.19 PE
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
130310 200 µg DKK1010
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description CD4 is a 55 kD protein, also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosin kinase, lck. The H129.19 antibody is able to block CD4 mediated cell adhesion and T cell activation. It is also useful for in vivo and in vitro depletion of CD4+ T cells. H129.19 antibody blocks binding of the anti-mouse CD4 mAbs GK1.5 and RM4-5, but not RM4-4 antibody. H129.19 antibody inhibits various responses of T helper cells to antigenic or mitogenic stimuli. Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse lymphocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: blocking of CD4+ T cell activation1-3, depletion. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays.

Application References
  1. Naquet P. 1985. Immunogenetics. 22:247-256.
  2. Pierres A. 1984. J. Immunol.. 132:2775-2782.
  3. Guo MW. 1995. Zygote. 3:65-73.
  4. Ghobrial RR. 1989. Clin Immunol Immunopathol. 52:486-506.
Product Citations
  1. Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
  2. Chen Y, et al. 2023. Adv Healthc Mater. 12:e2202085. PubMed
  3. Horkova V, et al. 2023. Nat Immunol. 24:174. PubMed
  4. Wang D, et al. 2023. Nat Commun. 14:2943. PubMed
  5. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
  6. Sá da Bandeira D, et al. 2022. Cell Rep. 40:111114. PubMed
  7. Zhang C, et al. 2022. Nat Commun. 13:3468. PubMed
  8. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  9. Oka Y, et al. 2020. Sci Adv. 6:. PubMed
  10. Jiang Y, et al. 2021. Nat Commun. 12:742. PubMed
  11. Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
  12. Chen Y, et al. 2023. Adv Healthc Mater. 12:e2202085. PubMed
  13. Horkova V, et al. 2023. Nat Immunol. 24:174. PubMed
  14. Wang D, et al. 2023. Nat Commun. 14:2943. PubMed
  15. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
  16. Sá da Bandeira D, et al. 2022. Cell Rep. 40:111114. PubMed
  17. Zhang C, et al. 2022. Nat Commun. 13:3468. PubMed
  18. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  19. Oka Y, et al. 2020. Sci Adv. 6:. PubMed
  20. Jiang Y, et al. 2021. Nat Commun. 12:742. PubMed
RRID
AB_2075573 (BioLegend Cat. No. 130310)

Antigen Details

Structure
Ig superfamily, 55 kD
Distribution
Majority of thymocytes, T cell subset
Function
TCR co-receptor, T cell activation
Ligand/Receptor
MHC class II molecule
Cell Type
Thymocytes, T cells, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.
4. Naquet P. 1985. Immunogenetics. 22:247-256.
5. Pierres A. 1984. J. Immunol.. 132:2775-2782.
6. Guo MW. 1995. Zygote. 3:65-73.
7. Ghobrial RR. 1989. Clin Immunol Immunopathol. 52:486-506.
Gene ID
12504 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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