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PE anti-mouse IL-17A Antibody PE anti-mouse IL-17A Antibody

Pricing & Availability
Clone
TC11-18H10.1 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-17, Cytotoxic T lymphocyte-associated antigen 8 (CTLA-8)
Isotype
Rat IgG1, κ
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Product Citations
publications
TC11-18H10.1_PE_041910
PMA/ionomycin-stimulated (5 hours) Th17 polarized C57BL/6 mouse CD4+ T cells surface stained with CD4 (GK1.5) APC, then intracellularly stained with TC11-18H10.1 PE
  • TC11-18H10.1_PE_041910
    PMA/ionomycin-stimulated (5 hours) Th17 polarized C57BL/6 mouse CD4+ T cells surface stained with CD4 (GK1.5) APC, then intracellularly stained with TC11-18H10.1 PE
  • TC11-18H10_PE_060806
    PMA (20 ng/ml) + ionomycin (1 µg/ml) -stimulated (6 hours + monensin, 2 µM) mouse thymoma cell line EL-4 intracellularly stained with TC11-18H10.1 PE
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
506903 25 µg DKK654
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506904 100 µg DKK1664
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Description

IL-17, also known as CTLA-8, is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpes virus Saimiri. IL-17 is produced by Th cells (Th17) that are distinct from the traditional Th1- and Th2-cell subsets. IL-23 plays an important role in triggering IL-17 production. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6 and IL-8 production in fibroblasts, activation of NF-κB, and costimulation of T cell proliferation. IL-17 is an essential inflammatory mediator in the development of autoimmune diseases. Neutralization of IL-17 with monoclonal antibody is able to ameliorate the disease course.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli expressed, recombinant mouse IL-17A
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The IL-17A antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

ELISA Capture3,4 and ELISPOT Capture5: The purified TC11-18H10.1 antibody is useful as the capture antibody in a sandwich ELISA, when used in conjunction with the biotinylated TC11-8H4 antibody (Cat. No. 507002) as the detecting antibody and recombinant mouse IL-17 (Cat. No. 576009) as the standard.
Flow Cytometry2-4,7,8,11,12: The TC11-18H10.1 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-17-producing cells within mixed cell populations.
Neutralization6,9: The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IL-17 bioactivity in vivo and in vitro (Cat. No. 506906).

Application References
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  202. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
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  204. Ogawa C et al. 2018. Cell reports. 25(1):19-28 . PubMed
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  206. Kuwahara M, et al. 2014. Nat Commun. 5:3555. PubMed
  207. Nagaoka M, et al. 2014. J Immunol. 193:2812. PubMed
  208. Yasuoka T, et al. 2016. PLoS One. 11: 0157395. PubMed
  209. Wagner V, et al. 2016. Sci Transl Med. 8: 366ra164. PubMed
  210. D Kim, S Lee, H Jun 2016. Immunol Cell Biol. 10.1038/icb.2016.98. PubMed
  211. Liu YJ, et al. 2020. Theranostics. 10:5225. PubMed
  212. Alissafi T, et al. 2020. Cell Metabolism. 32(4):591-604.e7. PubMed
  213. Britton GJ et al. 2019. Immunity. 50(1):212-224 . PubMed
  214. He J, et al. 2020. Cell Reports. 29(9):2718-2730.e6.. PubMed
  215. Zhao F, et al. 2022. Front Immunol. 13:873720. PubMed
  216. Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
  217. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  218. Kawakami R, et al. 2021. Immunity. 54(5):947-961.e8. PubMed
  219. Luo Y et al. 2019. Cell reports. 26(7):1869-1879 . PubMed
  220. Colliou N, et al. 2018. Gut Microbes. 9:279. PubMed
  221. Dar HY, et al. 2018. Sci Rep. 8:2503. PubMed
  222. Pu Y, et al. 2022. Transl Psychiatry. 12:223. PubMed
  223. Ni P, et al. 2014. Mol Immunol. 62:199. PubMed
  224. Niwa H, et al. 2009. Clin Immunol. 132:184. PubMed
  225. Wang J, et al. 2021. Nat Commun. 12:6198. PubMed
  226. Dar HY, et al. 2018. Bone Rep. 8:46. PubMed
  227. Bhattacharya D, et al. 2014. J Biol Chem. 289:16508. PubMed
  228. Mara AB, et al. 2022. NPJ Vaccines. 7:86. PubMed
  229. Yong L, et al. 2022. Nat Commun. 13:4255. PubMed
  230. Zeng Q, et al. 2022. Front Immunol. 13:740805. PubMed
  231. Takeshita Y, et al. 2021. Neurol Neuroimmunol Neuroinflamm. 8:. PubMed
  232. Ouyang W, et al. 2021. Invest Ophthalmol Vis Sci. 62:25:00. PubMed
  233. Bankoti R, et al. 2017. Sci Rep. 10.1038/s41598-017-12171-3. PubMed
  234. Castro–Dopico T, et al. 2020. Cell Reports. 32(1):107857. PubMed
  235. Tan YY, et al. 2021. Exp Ther Med. 22:1368. PubMed
  236. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  237. Vaena S, et al. 2021. Cell Reports. 35(5):109076. PubMed
  238. Alhosaini K, et al. 2021. Brain Sci. :11. PubMed
  239. Wen M, et al. 2022. JCI Insight. Online ahead of print. PubMed
  240. Kawashima H, et al. 2013. J Immunol. 191:3614. PubMed
  241. Luck H, et al. 2015. Cell Metab. 21 527 . PubMed
  242. Park S, et al. 2011. J Immunol. 187:5813. PubMed
  243. Chatterjee S et al. 2017. Cell metabolism. 27(1):85-100 . PubMed
  244. Lin YW, et al. 2022. Mol Med Rep. 26:. PubMed
  245. Chou T, et al. 2016. Nat Commun. 7:11904. PubMed
  246. Takeuchi H, et al. 2013. J Immunol. 191:3725. PubMed
  247. Xiao S, et al. 2020. Cell Reports. 32(2):107892. PubMed
  248. Van Dis E, et al. 2018. Cell Rep. 23:1435. PubMed
  249. Ramakrishnan SK, et al. 2019. Nat Commun. 10:660. PubMed
  250. Katagiri T, et al. 2019. Mucosal Immunol. 12:p1104. PubMed
  251. Zeng Q, et al. 2022. iScience. 25:105151. PubMed
  252. Gorman JA, et al. 2019. Front Immunol. 10:44. PubMed
  253. Ouyang S, et al. 2019. J Immunol. 202:1441. PubMed
  254. Ghorbani S, et al. 2022. Nat Commun. 13:2445. PubMed
  255. Derada Troletti C, et al. 2021. Cell Reports. 35(9):109201. PubMed
  256. Wang G, et al. 2021. Cell Host Microbe. 29(5):777-791.e6. PubMed
  257. Wu B, et al. 2021. Immunity. 54(2):308-323.e6. PubMed
  258. Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
  259. Sanchez M et al. 2017. Cell host & microbe. 22(4):543-551 . PubMed
  260. Luck H, et al. 2019. Nat Commun. 10:3650. PubMed
  261. Metidji A et al. 2018. Immunity. 49(2):353-362 . PubMed
  262. Kang J, Lee J, Chang J 2016. PLoS One. 11: 0157015. PubMed
  263. Ehirchiou D, et al. 2007. J Exp Med . 204:1519. PubMed
  264. Lai Y, et al. 2022. Clin Transl Med. 12:e999. PubMed
  265. Miura N, et al. 2017. Mol Ther. 10.1016/j.ymthe.2017.01.020. PubMed
  266. Kang YH, et al. 2019. Nat Commun. 10:912. PubMed
  267. Karanika S, et al. 2022. Front Immunol. 13:972266. PubMed
  268. Li X, et al. 2016. MBio. 7: 02232-15. PubMed
  269. Mizutani N, et al. 2012. J Immunol. 188:5694. PubMed
  270. Suhail A, et al. 2019. Cell Rep. 29:3522. PubMed
  271. Zhang Y, et al. 2021. Commun Biol. 344:4. PubMed
  272. Tian D, et al. 2019. Nat Commun. 10:4246. PubMed
  273. Castro‐Dopico T et al. 2019. Immunity. 50(4):1099-1114 . PubMed
  274. Zegarra‐Ruiz DF et al. 2018. Cell host & microbe. 25(1):113-127 . PubMed
  275. Jeon Y, et al. 2015. PLoS One. 10: e0139845. PubMed
  276. Hao L, et al. 2021. Br J Pharmacol. 178:4726. PubMed
  277. Yue T, et al. 2022. Cell Death Differ. 29:218. PubMed
  278. Huang LJ, et al. 2021. Immunity. 54(8):1728-1744.e7. PubMed
  279. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  280. Yu H, et al. 2015. PLoS One. 10: 0143001. PubMed
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  284. Tang L, et al. 2022. Front Immunol. 12:770402. PubMed
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  286. Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
  287. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
  288. Toriyama K, et al. 2020. Commun Biol. 0.398611111. PubMed
  289. Wang J, et al. 2020. Cell. 183(7):1867-1883.e26. PubMed
  290. Li J, et al. 2012. Arthritis Rheum. 64:1098. PubMed
  291. Li C, et al. 2014. J Immunol. 192:1425. PubMed
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RRID
AB_315463 (BioLegend Cat. No. 506903)
AB_315464 (BioLegend Cat. No. 506904)

Antigen Details

Structure
Cytokine; dimer; 15 kD (Mammalian).
Bioactivity
Secretion of IL-6, IL-8, G-CSF, prostaglandin E2 by epithelial, endothelial or fibroblastic cells; stimulates cell migration, cord formation, and IL-6 secretion by stromal cells
Cell Sources
CD4+ memory T cells
Cell Targets
Fibroblasts, epithelial and endothelial cells, stromal cells
Receptors
IL-17R (CD217)
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Numasaki M, et al. 2002. Blood 101:2620.
3. Fossiez F, et al. 1996. J. Exp. Med. 183:2593.
4. Yao Z, et al. 1997. Cytokine 9:794.
5. Dong C. 2006. Nat. Rev. Immunol. 6:329.
6. Hofstetter HH, et al. 2005 Cell. Immunol. 237:123.

Gene ID
16171 View all products for this Gene ID
UniProt
View information about IL-17A on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Does the anti-mouse IL-17 antibody (clone TC11-18H10.1) recognize the A or F isoform?
Clone TC11-18H10.1 recognizes IL-17A isoform, but it also recognizes the IL-17A/F heterodimer via IL-17A binding.
Go To Top Version: 2    Revision Date: 10/16/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Pricing & Availability
Clone
TC11-18H10.1 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-17, Cytotoxic T lymphocyte-associated antigen 8 (CTLA-8)
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
TC11-18H10.1_PE_041910
PMA/ionomycin-stimulated (5 hours) Th17 polarized C57BL/6 mouse CD4+ T cells surface stained with CD4 (GK1.5) APC, then intracellularly stained with TC11-18H10.1 PE
  • TC11-18H10.1_PE_041910
    PMA/ionomycin-stimulated (5 hours) Th17 polarized C57BL/6 mouse CD4+ T cells surface stained with CD4 (GK1.5) APC, then intracellularly stained with TC11-18H10.1 PE
  • TC11-18H10_PE_060806
    PMA (20 ng/ml) + ionomycin (1 µg/ml) -stimulated (6 hours + monensin, 2 µM) mouse thymoma cell line EL-4 intracellularly stained with TC11-18H10.1 PE
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
506903 25 µg DKK654
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506904 100 µg DKK1664
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Description

IL-17, also known as CTLA-8, is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpes virus Saimiri. IL-17 is produced by Th cells (Th17) that are distinct from the traditional Th1- and Th2-cell subsets. IL-23 plays an important role in triggering IL-17 production. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6 and IL-8 production in fibroblasts, activation of NF-κB, and costimulation of T cell proliferation. IL-17 is an essential inflammatory mediator in the development of autoimmune diseases. Neutralization of IL-17 with monoclonal antibody is able to ameliorate the disease course.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli expressed, recombinant mouse IL-17A
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The IL-17A antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

ELISA Capture3,4 and ELISPOT Capture5: The purified TC11-18H10.1 antibody is useful as the capture antibody in a sandwich ELISA, when used in conjunction with the biotinylated TC11-8H4 antibody (Cat. No. 507002) as the detecting antibody and recombinant mouse IL-17 (Cat. No. 576009) as the standard.
Flow Cytometry2-4,7,8,11,12: The TC11-18H10.1 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-17-producing cells within mixed cell populations.
Neutralization6,9: The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IL-17 bioactivity in vivo and in vitro (Cat. No. 506906).

Application References
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  158. Tian WJ, et al. 2021. Clin Exp Pharmacol Physiol. 48:902. PubMed
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  189. Angiari S, et al. 2020. Cell Metab. 31:391. PubMed
  190. Pan Y, et al. 2021. Front Cell Neurosci. 15:664312. PubMed
  191. Yang C, et al. 2018. J Immunol. 200:1316. PubMed
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  193. Kenichi Shimada et al. 2018. Immunity. 49(5):873-885 . PubMed
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RRID
AB_315463 (BioLegend Cat. No. 506903)
AB_315464 (BioLegend Cat. No. 506904)

Antigen Details

Structure
Cytokine; dimer; 15 kD (Mammalian).
Bioactivity
Secretion of IL-6, IL-8, G-CSF, prostaglandin E2 by epithelial, endothelial or fibroblastic cells; stimulates cell migration, cord formation, and IL-6 secretion by stromal cells
Cell Sources
CD4+ memory T cells
Cell Targets
Fibroblasts, epithelial and endothelial cells, stromal cells
Receptors
IL-17R (CD217)
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Numasaki M, et al. 2002. Blood 101:2620.
3. Fossiez F, et al. 1996. J. Exp. Med. 183:2593.
4. Yao Z, et al. 1997. Cytokine 9:794.
5. Dong C. 2006. Nat. Rev. Immunol. 6:329.
6. Hofstetter HH, et al. 2005 Cell. Immunol. 237:123.

Gene ID
16171 View all products for this Gene ID
UniProt
View information about IL-17A on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Does the anti-mouse IL-17 antibody (clone TC11-18H10.1) recognize the A or F isoform?
Clone TC11-18H10.1 recognizes IL-17A isoform, but it also recognizes the IL-17A/F heterodimer via IL-17A binding.
Go To Top Version: 2    Revision Date: 10/16/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

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