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PE/Cyanine7 anti-mouse CD25 Antibody PE/Cyanine7 anti-mouse CD25 Antibody

Pricing & Availability
Clone
PC61 (See other available formats)
Regulatory Status
RUO
Other Names
IL-2Rα, Ly-43, p55, Tac
Isotype
Rat IgG1, λ
Ave. Rating
Submit a Review
Product Citations
publications
PC61_PECy7_080607
Con A-stimulated (2 days) BALB/c mouse splenocytes stained with PC61 PE/Cyanine7
  • PC61_PECy7_080607
    Con A-stimulated (2 days) BALB/c mouse splenocytes stained with PC61 PE/Cyanine7
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
102015 25 µg DKK517
Check Availability


Need larger quantities of this item?
Request Bulk Quote
102016 100 µg DKK1254
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD25 is a 55 kD glycoprotein also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132 (common γ chain), CD25 forms the high affinity signaling IL-2 receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-2-dependent cytolytic mouse T-cell clone B6.1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, in vitro blocking of IL-2 binding to low- and high-affinity receptors1-4, growth inhibition of IL-2-dependent T-cell lines1-4, in vivo depletion of CD25+CD4+ Treg cells5-8,10, and immunohistochemical staining of acetone-fixed frozen sections2. PC61 antibody recognizes a different epitope than 3C7 antibody (Cat. No. 101902). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 102040) with endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered. 

Application References
  1. Lowenthal JW, et al. 1985. Nature 315:669. (IP, Block)
  2. Ceredig R, et al. 1985. Nature 314:98. (IP, IHC, Block)
  3. Lowenthal JW, et al. 1985. J. Immunol. 135:3988. (Block)
  4. Moreau JL, et al. 1987. Eur. J. Immunol. 17:929. (Block)
  5. Takahashi T, et al. 2000. J. Exp. Med. 192:303. (Deplete)
  6. Onizuka S, et al. 1999. Cancer Res. 59:3128. (Deplete)
  7. Lei TC, et al. 2005. Blood 105:4865. (Deplete)
  8. Pasare C, et al. 2004. Immunity 21:733. (Deplete)
  9. León-Ponte M, et al. 2007. Blood 109:3139.
  10. Cao OW, et al. 2007. Blood doi:10.1182/blood-2007-02-073304. (Deplete)
  11. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719.
  12. Liu F, et al. 2011. Arch Toxicol. 85:1383. PubMed
  13. Anguela XM, et al. 2013. Diabetes. 62:551. PubMed
Product Citations
  1. Kawabe T, et al. 2020. Nat Commun. 2.795833333. PubMed
  2. Gao Y, et al. 2023. iScience. 26:106729. PubMed
  3. Denny L, et al. 2021. Clin Transl Immunology. 10:e1234. PubMed
  4. Thomas R, et al. 2021. Immunology. 163:478. PubMed
  5. Dölz M, et al. 2022. iScience. 25:105372. PubMed
  6. Tan TCJ, et al. 2022. Nat Commun. 13:7796. PubMed
  7. Wang R, et al. 2023. Nat Biomed Eng. 7:38. PubMed
  8. Horkova V, et al. 2023. Nat Immunol. 24:174. PubMed
  9. Sieow JL, et al. 2023. Int J Mol Sci. 24: . PubMed
  10. Glaubitz J, et al. 2023. Gut. :. PubMed
  11. Zhao Q, et al. 2023. Nutrients. 15: . PubMed
  12. Elliot TAE, et al. 2022. Discov Immunol. 1:kyac009. PubMed
  13. Cao Y, et al. 2023. J Inflamm Res. 16:343. PubMed
  14. Tunali G, et al. 2023. J Clin Invest. :. PubMed
  15. Hajjar S, et al. 2022. Cell Death Differ. 29:585. PubMed
  16. Wilden A, et al. 2022. Front Immunol. 13:991295. PubMed
  17. Gruber R, et al. 2014. J Neurosci. 34:16320. PubMed
  18. Fu Z, et al. 2019. Cell Rep. 28:159. PubMed
  19. Wilfahrt D, et al. 2021. Elife. 10:. PubMed
  20. Andrade MS, et al. 2022. JCI Insight. 7:. PubMed
  21. Prasad M, et al. 2020. Cell Mol Immunol. . PubMed
  22. Hiraishi Y, et al. 2018. Sci Rep. 8:18052. PubMed
  23. Isoda T et al. 2017. Cell. 171(1):103-119 . PubMed
  24. Schmidleithner L et al. 2019. Immunity. 50(5):1232-1248 . PubMed
  25. Achmus L, et al. 2020. Front Neurol. 11:577971. PubMed
  26. Liu Q, et al. 2021. Cell Death Dis. 12:240. PubMed
  27. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  28. Elisabeth Schrumpf, Xiaojun Jiang 2017. Physiol Rep. 10.14814/phy2.13117. PubMed
  29. Petursdottir D, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01699. PubMed
  30. Aqel SI, et al. 2021. JCI Insight. 6:e142376. PubMed
  31. Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed
  32. Yang W, et al. 2020. Mucosal Immunol. 13:788. PubMed
  33. Marfil-Garza BA, et al. 2022. Am J Transplant. 22:1101. PubMed
  34. Paprckova D, et al. 2022. Front Immunol. 13:1009198. PubMed
  35. Suah AN, et al. 2021. J Clin Invest. 131:. PubMed
  36. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  37. Martinikova AS, et al. 2020. Cells. 9:00. PubMed
  38. Shan M et al. 2018. Immunity. 49(4):709-724 . PubMed
  39. Xiong Y, et al. 2018. Int J Clin Exp Pathol. 3.803472222. PubMed
  40. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
  41. Benechet AP, et al. 2019. Nature. 574:200. PubMed
  42. Maluski M, et al. 2019. J Clin Invest. 129:5108. PubMed
  43. Trompette A, et al. 2022. Mucosal Immunol. :. PubMed
  44. Ng SS, et al. 2020. Nat Immunol. 21:1205. PubMed
  45. Knizkova D, et al. 2022. Nat Immunol. 23:1644. PubMed
  46. Sido J, et al. 2015. J Leukoc Biol. 98: 435-447. PubMed
  47. Horkova V, et al. 2020. Cell Reports. 30(5):1504-1514.e7.. PubMed
  48. Sinclair LV, et al. 2020. Immunometabolism. 2:e200029. PubMed
  49. Ku C, et al. 2015. Genes Dev. 29: 1930-1941. PubMed
  50. De Simone G, et al. 2021. Immunity. :. PubMed
  51. Coronel MM, et al. 2020. Sci Adv. 6:eaba5573. PubMed
  52. Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
  53. Biton M et al. 2018. Cell. 175(5):1307-1320 . PubMed
  54. Moore MJ et al. 2018. eLife. 7 pii: e33057. PubMed
  55. De Ponte Conti B, et al. 2021. Elife. 10:. PubMed
  56. Daneshmandi S, et al. 2021. Elife. 10:. PubMed
  57. Lindner J, et al. 2014. J Immunol. 192:4483. PubMed
  58. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  59. Zhai X, et al. 2021. Sci Adv. 7:eabk0490. PubMed
  60. Dionisio-Santos DA, et al. 2021. Front Neurosci. 15:758677. PubMed
  61. Evgin L, et al. 2020. Nat Commun. 2.671527778. PubMed
  62. Perry JL, et al. 2020. ACS Nano. 5.583333333. PubMed
  63. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  64. Qadir AS, et al. 2021. iScience. 24:103348. PubMed
  65. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  66. Moreau H, et al. 2015. Proc Natl Acad Sci U S A. 112: 12151-12156. PubMed
  67. Fernández-Orth J, et al. 2020. Eur J Immunol. . PubMed
  68. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  69. Li D, et al. 2020. Immunohorizons. 0.661805556. PubMed
  70. Mairhofer D, et al. 2015. J Invest Dermatol. 135: 2785-93. PubMed
  71. Rao R, et al. 2015. Toxicol Sci. 144:284. PubMed
  72. Porrello A, et al. 2018. Nat Commun. 9:1988. PubMed
  73. Li J, et al. 2021. Cell Rep. 37:110124. PubMed
  74. Fleskens V, et al. 2019. Cell Rep. 26:3600. PubMed
  75. Teng F, et al. 2021. Cell Rep. 37:110051. PubMed
  76. Kashiwakura Y, et al. 2020. Clinical & Experimental Immunology. 202(1):119-135. PubMed
  77. Steinmann S, et al. 2020. Sci Rep. 1.160416667. PubMed
  78. Kastenschmidt JM, et al. 2021. Cell Rep. 35:108997. PubMed
  79. Chen Y, et al. 2021. Braz J Med Biol Res. 54:e9570. PubMed
  80. Liu A, et al. 2022. Front Cell Infect Microbiol. 12:960208. PubMed
  81. Zaiss M, et al. 2015. Immunity. 43: 998-1010. PubMed
  82. Nabekura T, et al. 2020. Immunity. 96:52. PubMed
  83. Kawabe T, et al. 2020. Nat Commun. 2.795833333. PubMed
  84. Gao Y, et al. 2023. iScience. 26:106729. PubMed
  85. Denny L, et al. 2021. Clin Transl Immunology. 10:e1234. PubMed
  86. Thomas R, et al. 2021. Immunology. 163:478. PubMed
  87. Dölz M, et al. 2022. iScience. 25:105372. PubMed
  88. Tan TCJ, et al. 2022. Nat Commun. 13:7796. PubMed
  89. Wang R, et al. 2023. Nat Biomed Eng. 7:38. PubMed
  90. Horkova V, et al. 2023. Nat Immunol. 24:174. PubMed
  91. Sieow JL, et al. 2023. Int J Mol Sci. 24: . PubMed
  92. Glaubitz J, et al. 2023. Gut. :. PubMed
  93. Zhao Q, et al. 2023. Nutrients. 15: . PubMed
  94. Elliot TAE, et al. 2022. Discov Immunol. 1:kyac009. PubMed
  95. Cao Y, et al. 2023. J Inflamm Res. 16:343. PubMed
  96. Tunali G, et al. 2023. J Clin Invest. :. PubMed
  97. Hajjar S, et al. 2022. Cell Death Differ. 29:585. PubMed
  98. Wilden A, et al. 2022. Front Immunol. 13:991295. PubMed
  99. Gruber R, et al. 2014. J Neurosci. 34:16320. PubMed
  100. Fu Z, et al. 2019. Cell Rep. 28:159. PubMed
  101. Wilfahrt D, et al. 2021. Elife. 10:. PubMed
  102. Andrade MS, et al. 2022. JCI Insight. 7:. PubMed
  103. Prasad M, et al. 2020. Cell Mol Immunol. . PubMed
  104. Hiraishi Y, et al. 2018. Sci Rep. 8:18052. PubMed
  105. Isoda T et al. 2017. Cell. 171(1):103-119 . PubMed
  106. Schmidleithner L et al. 2019. Immunity. 50(5):1232-1248 . PubMed
  107. Achmus L, et al. 2020. Front Neurol. 11:577971. PubMed
  108. Liu Q, et al. 2021. Cell Death Dis. 12:240. PubMed
  109. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  110. Elisabeth Schrumpf, Xiaojun Jiang 2017. Physiol Rep. 10.14814/phy2.13117. PubMed
  111. Petursdottir D, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01699. PubMed
  112. Aqel SI, et al. 2021. JCI Insight. 6:e142376. PubMed
  113. Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed
  114. Yang W, et al. 2020. Mucosal Immunol. 13:788. PubMed
  115. Marfil-Garza BA, et al. 2022. Am J Transplant. 22:1101. PubMed
  116. Paprckova D, et al. 2022. Front Immunol. 13:1009198. PubMed
  117. Suah AN, et al. 2021. J Clin Invest. 131:. PubMed
  118. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  119. Martinikova AS, et al. 2020. Cells. 9:00. PubMed
  120. Shan M et al. 2018. Immunity. 49(4):709-724 . PubMed
  121. Xiong Y, et al. 2018. Int J Clin Exp Pathol. 3.803472222. PubMed
  122. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
  123. Benechet AP, et al. 2019. Nature. 574:200. PubMed
  124. Maluski M, et al. 2019. J Clin Invest. 129:5108. PubMed
  125. Trompette A, et al. 2022. Mucosal Immunol. :. PubMed
  126. Ng SS, et al. 2020. Nat Immunol. 21:1205. PubMed
  127. Knizkova D, et al. 2022. Nat Immunol. 23:1644. PubMed
  128. Sido J, et al. 2015. J Leukoc Biol. 98: 435-447. PubMed
  129. Horkova V, et al. 2020. Cell Reports. 30(5):1504-1514.e7.. PubMed
  130. Sinclair LV, et al. 2020. Immunometabolism. 2:e200029. PubMed
  131. Ku C, et al. 2015. Genes Dev. 29: 1930-1941. PubMed
  132. De Simone G, et al. 2021. Immunity. :. PubMed
  133. Coronel MM, et al. 2020. Sci Adv. 6:eaba5573. PubMed
  134. Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
  135. Biton M et al. 2018. Cell. 175(5):1307-1320 . PubMed
  136. Moore MJ et al. 2018. eLife. 7 pii: e33057. PubMed
  137. De Ponte Conti B, et al. 2021. Elife. 10:. PubMed
  138. Daneshmandi S, et al. 2021. Elife. 10:. PubMed
  139. Lindner J, et al. 2014. J Immunol. 192:4483. PubMed
  140. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  141. Zhai X, et al. 2021. Sci Adv. 7:eabk0490. PubMed
  142. Dionisio-Santos DA, et al. 2021. Front Neurosci. 15:758677. PubMed
  143. Evgin L, et al. 2020. Nat Commun. 2.671527778. PubMed
  144. Perry JL, et al. 2020. ACS Nano. 5.583333333. PubMed
  145. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  146. Qadir AS, et al. 2021. iScience. 24:103348. PubMed
  147. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  148. Moreau H, et al. 2015. Proc Natl Acad Sci U S A. 112: 12151-12156. PubMed
  149. Fernández-Orth J, et al. 2020. Eur J Immunol. . PubMed
  150. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  151. Li D, et al. 2020. Immunohorizons. 0.661805556. PubMed
  152. Mairhofer D, et al. 2015. J Invest Dermatol. 135: 2785-93. PubMed
  153. Rao R, et al. 2015. Toxicol Sci. 144:284. PubMed
  154. Porrello A, et al. 2018. Nat Commun. 9:1988. PubMed
  155. Li J, et al. 2021. Cell Rep. 37:110124. PubMed
  156. Fleskens V, et al. 2019. Cell Rep. 26:3600. PubMed
  157. Teng F, et al. 2021. Cell Rep. 37:110051. PubMed
  158. Kashiwakura Y, et al. 2020. Clinical & Experimental Immunology. 202(1):119-135. PubMed
  159. Steinmann S, et al. 2020. Sci Rep. 1.160416667. PubMed
  160. Kastenschmidt JM, et al. 2021. Cell Rep. 35:108997. PubMed
  161. Chen Y, et al. 2021. Braz J Med Biol Res. 54:e9570. PubMed
  162. Liu A, et al. 2022. Front Cell Infect Microbiol. 12:960208. PubMed
  163. Zaiss M, et al. 2015. Immunity. 43: 998-1010. PubMed
  164. Nabekura T, et al. 2020. Immunity. 96:52. PubMed
RRID
AB_312864 (BioLegend Cat. No. 102015)
AB_312865 (BioLegend Cat. No. 102016)

Antigen Details

Structure
Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132), 55 kD
Distribution

Activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells

Function
IL-2 receptor
Ligand/Receptor
IL-2
Cell Type
B cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References
  1. Taniguchi T, et al. 1993. Cell 73:5-8.
  2. Waldmann TA. 1991. J Biol Chem. 266:2681-4.
  3. Read S, et al. 2000. J Exp Med. 192:295-302.
  4. Lowenthal JW, et al. 1985. J Immunol. 135:3988-94.
Gene ID
16184 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD25 Reagents Request Custom Conjugation
Description Clone Applications
PE/Cyanine7 anti-mouse CD25 PC61 FC
PE/Dazzle™ 594 anti-mouse CD25 PC61 FC
Brilliant Violet 711™ anti-mouse CD25 PC61 FC
Brilliant Violet 785™ anti-mouse CD25 PC61 FC
Alexa Fluor® 594 anti-mouse CD25 PC61 IHC-F
APC/Fire™ 750 anti-mouse CD25 PC61 FC
TotalSeq™-A0097 anti-mouse CD25 PC61 PG
KIRAVIA Blue 520™ anti-mouse CD25 PC61 FC
TotalSeq™-B0097 anti-mouse CD25 PC61 PG
TotalSeq™-C0097 anti-mouse CD25 PC61 PG
Spark NIR™ 685 anti-mouse CD25 Antibody PC61 FC
APC anti-mouse CD25 PC61 FC
Biotin anti-mouse CD25 PC61 FC
FITC anti-mouse CD25 PC61 FC
PE anti-mouse CD25 PC61 FC
PE/Cyanine5 anti-mouse CD25 PC61 FC
Purified anti-mouse CD25 PC61 FC,IHC-F,IP,Block,Depletion
Alexa Fluor® 488 anti-mouse CD25 PC61 FC
Alexa Fluor® 647 anti-mouse CD25 PC61 FC
Pacific Blue™ anti-mouse CD25 PC61 FC
Alexa Fluor® 700 anti-mouse CD25 PC61 FC
APC/Cyanine7 anti-mouse CD25 PC61 FC
PerCP/Cyanine5.5 anti-mouse CD25 PC61 FC
PerCP anti-mouse CD25 PC61 FC
Brilliant Violet 421™ anti-mouse CD25 PC61 FC
Brilliant Violet 605™ anti-mouse CD25 PC61 FC
Brilliant Violet 650™ anti-mouse CD25 PC61 FC
Ultra-LEAF™ Purified anti-mouse CD25 PC61 FC,IHC-F,IP,Block,Depletion
Brilliant Violet 510™ anti-mouse CD25 PC61 FC
PE/Fire™ 640 anti-mouse CD25 PC61 FC
Spark YG™ 581 anti-mouse CD25 PC61 FC
APC/Fire™ 810 anti-mouse CD25 PC61 FC
Brilliant Violet 750™ anti-mouse CD25 PC61 FC
PerCP/Fire™ 780 anti-mouse CD25 PC61 FC
PE/Fire™ 700 anti-mouse CD25 PC61 FC
Spark PLUS UV395™ anti-mouse CD25 PC61 FC
Spark Blue™ 574 anti-mouse CD25 (Flexi-Fluor™) PC61 FC
PerCP/Fire™ 806 anti-mouse CD25 Antibody PC61 FC
Spark PLUS B550™ anti-mouse CD25 PC61 FC
Spark YG™ 593 anti-mouse CD25 (Flexi-Fluor™) Antibody PC61 FC
Spark UV™ 387 anti-mouse CD25 (Flexi-Fluor™) PC61 FC
Spark PLUS V475™ anti-mouse CD25 Antibody PC61 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Pricing & Availability
Clone
PC61 (See other available formats)
Regulatory Status
RUO
Other Names
IL-2Rα, Ly-43, p55, Tac
Isotype
Rat IgG1, λ
Ave. Rating
Submit a Review
Product Citations
publications
PC61_PECy7_080607
Con A-stimulated (2 days) BALB/c mouse splenocytes stained with PC61 PE/Cyanine7
  • PC61_PECy7_080607
    Con A-stimulated (2 days) BALB/c mouse splenocytes stained with PC61 PE/Cyanine7
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
102015 25 µg DKK517
Check Availability


Need larger quantities of this item?
Request Bulk Quote
102016 100 µg DKK1254
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD25 is a 55 kD glycoprotein also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132 (common γ chain), CD25 forms the high affinity signaling IL-2 receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-2-dependent cytolytic mouse T-cell clone B6.1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, in vitro blocking of IL-2 binding to low- and high-affinity receptors1-4, growth inhibition of IL-2-dependent T-cell lines1-4, in vivo depletion of CD25+CD4+ Treg cells5-8,10, and immunohistochemical staining of acetone-fixed frozen sections2. PC61 antibody recognizes a different epitope than 3C7 antibody (Cat. No. 101902). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 102040) with endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered. 

Application References
  1. Lowenthal JW, et al. 1985. Nature 315:669. (IP, Block)
  2. Ceredig R, et al. 1985. Nature 314:98. (IP, IHC, Block)
  3. Lowenthal JW, et al. 1985. J. Immunol. 135:3988. (Block)
  4. Moreau JL, et al. 1987. Eur. J. Immunol. 17:929. (Block)
  5. Takahashi T, et al. 2000. J. Exp. Med. 192:303. (Deplete)
  6. Onizuka S, et al. 1999. Cancer Res. 59:3128. (Deplete)
  7. Lei TC, et al. 2005. Blood 105:4865. (Deplete)
  8. Pasare C, et al. 2004. Immunity 21:733. (Deplete)
  9. León-Ponte M, et al. 2007. Blood 109:3139.
  10. Cao OW, et al. 2007. Blood doi:10.1182/blood-2007-02-073304. (Deplete)
  11. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719.
  12. Liu F, et al. 2011. Arch Toxicol. 85:1383. PubMed
  13. Anguela XM, et al. 2013. Diabetes. 62:551. PubMed
Product Citations
  1. Kawabe T, et al. 2020. Nat Commun. 2.795833333. PubMed
  2. Gao Y, et al. 2023. iScience. 26:106729. PubMed
  3. Denny L, et al. 2021. Clin Transl Immunology. 10:e1234. PubMed
  4. Thomas R, et al. 2021. Immunology. 163:478. PubMed
  5. Dölz M, et al. 2022. iScience. 25:105372. PubMed
  6. Tan TCJ, et al. 2022. Nat Commun. 13:7796. PubMed
  7. Wang R, et al. 2023. Nat Biomed Eng. 7:38. PubMed
  8. Horkova V, et al. 2023. Nat Immunol. 24:174. PubMed
  9. Sieow JL, et al. 2023. Int J Mol Sci. 24: . PubMed
  10. Glaubitz J, et al. 2023. Gut. :. PubMed
  11. Zhao Q, et al. 2023. Nutrients. 15: . PubMed
  12. Elliot TAE, et al. 2022. Discov Immunol. 1:kyac009. PubMed
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  15. Hajjar S, et al. 2022. Cell Death Differ. 29:585. PubMed
  16. Wilden A, et al. 2022. Front Immunol. 13:991295. PubMed
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  45. Knizkova D, et al. 2022. Nat Immunol. 23:1644. PubMed
  46. Sido J, et al. 2015. J Leukoc Biol. 98: 435-447. PubMed
  47. Horkova V, et al. 2020. Cell Reports. 30(5):1504-1514.e7.. PubMed
  48. Sinclair LV, et al. 2020. Immunometabolism. 2:e200029. PubMed
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  50. De Simone G, et al. 2021. Immunity. :. PubMed
  51. Coronel MM, et al. 2020. Sci Adv. 6:eaba5573. PubMed
  52. Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
  53. Biton M et al. 2018. Cell. 175(5):1307-1320 . PubMed
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  57. Lindner J, et al. 2014. J Immunol. 192:4483. PubMed
  58. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  59. Zhai X, et al. 2021. Sci Adv. 7:eabk0490. PubMed
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  61. Evgin L, et al. 2020. Nat Commun. 2.671527778. PubMed
  62. Perry JL, et al. 2020. ACS Nano. 5.583333333. PubMed
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  64. Qadir AS, et al. 2021. iScience. 24:103348. PubMed
  65. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  66. Moreau H, et al. 2015. Proc Natl Acad Sci U S A. 112: 12151-12156. PubMed
  67. Fernández-Orth J, et al. 2020. Eur J Immunol. . PubMed
  68. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  69. Li D, et al. 2020. Immunohorizons. 0.661805556. PubMed
  70. Mairhofer D, et al. 2015. J Invest Dermatol. 135: 2785-93. PubMed
  71. Rao R, et al. 2015. Toxicol Sci. 144:284. PubMed
  72. Porrello A, et al. 2018. Nat Commun. 9:1988. PubMed
  73. Li J, et al. 2021. Cell Rep. 37:110124. PubMed
  74. Fleskens V, et al. 2019. Cell Rep. 26:3600. PubMed
  75. Teng F, et al. 2021. Cell Rep. 37:110051. PubMed
  76. Kashiwakura Y, et al. 2020. Clinical & Experimental Immunology. 202(1):119-135. PubMed
  77. Steinmann S, et al. 2020. Sci Rep. 1.160416667. PubMed
  78. Kastenschmidt JM, et al. 2021. Cell Rep. 35:108997. PubMed
  79. Chen Y, et al. 2021. Braz J Med Biol Res. 54:e9570. PubMed
  80. Liu A, et al. 2022. Front Cell Infect Microbiol. 12:960208. PubMed
  81. Zaiss M, et al. 2015. Immunity. 43: 998-1010. PubMed
  82. Nabekura T, et al. 2020. Immunity. 96:52. PubMed
  83. Kawabe T, et al. 2020. Nat Commun. 2.795833333. PubMed
  84. Gao Y, et al. 2023. iScience. 26:106729. PubMed
  85. Denny L, et al. 2021. Clin Transl Immunology. 10:e1234. PubMed
  86. Thomas R, et al. 2021. Immunology. 163:478. PubMed
  87. Dölz M, et al. 2022. iScience. 25:105372. PubMed
  88. Tan TCJ, et al. 2022. Nat Commun. 13:7796. PubMed
  89. Wang R, et al. 2023. Nat Biomed Eng. 7:38. PubMed
  90. Horkova V, et al. 2023. Nat Immunol. 24:174. PubMed
  91. Sieow JL, et al. 2023. Int J Mol Sci. 24: . PubMed
  92. Glaubitz J, et al. 2023. Gut. :. PubMed
  93. Zhao Q, et al. 2023. Nutrients. 15: . PubMed
  94. Elliot TAE, et al. 2022. Discov Immunol. 1:kyac009. PubMed
  95. Cao Y, et al. 2023. J Inflamm Res. 16:343. PubMed
  96. Tunali G, et al. 2023. J Clin Invest. :. PubMed
  97. Hajjar S, et al. 2022. Cell Death Differ. 29:585. PubMed
  98. Wilden A, et al. 2022. Front Immunol. 13:991295. PubMed
  99. Gruber R, et al. 2014. J Neurosci. 34:16320. PubMed
  100. Fu Z, et al. 2019. Cell Rep. 28:159. PubMed
  101. Wilfahrt D, et al. 2021. Elife. 10:. PubMed
  102. Andrade MS, et al. 2022. JCI Insight. 7:. PubMed
  103. Prasad M, et al. 2020. Cell Mol Immunol. . PubMed
  104. Hiraishi Y, et al. 2018. Sci Rep. 8:18052. PubMed
  105. Isoda T et al. 2017. Cell. 171(1):103-119 . PubMed
  106. Schmidleithner L et al. 2019. Immunity. 50(5):1232-1248 . PubMed
  107. Achmus L, et al. 2020. Front Neurol. 11:577971. PubMed
  108. Liu Q, et al. 2021. Cell Death Dis. 12:240. PubMed
  109. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  110. Elisabeth Schrumpf, Xiaojun Jiang 2017. Physiol Rep. 10.14814/phy2.13117. PubMed
  111. Petursdottir D, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01699. PubMed
  112. Aqel SI, et al. 2021. JCI Insight. 6:e142376. PubMed
  113. Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed
  114. Yang W, et al. 2020. Mucosal Immunol. 13:788. PubMed
  115. Marfil-Garza BA, et al. 2022. Am J Transplant. 22:1101. PubMed
  116. Paprckova D, et al. 2022. Front Immunol. 13:1009198. PubMed
  117. Suah AN, et al. 2021. J Clin Invest. 131:. PubMed
  118. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  119. Martinikova AS, et al. 2020. Cells. 9:00. PubMed
  120. Shan M et al. 2018. Immunity. 49(4):709-724 . PubMed
  121. Xiong Y, et al. 2018. Int J Clin Exp Pathol. 3.803472222. PubMed
  122. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
  123. Benechet AP, et al. 2019. Nature. 574:200. PubMed
  124. Maluski M, et al. 2019. J Clin Invest. 129:5108. PubMed
  125. Trompette A, et al. 2022. Mucosal Immunol. :. PubMed
  126. Ng SS, et al. 2020. Nat Immunol. 21:1205. PubMed
  127. Knizkova D, et al. 2022. Nat Immunol. 23:1644. PubMed
  128. Sido J, et al. 2015. J Leukoc Biol. 98: 435-447. PubMed
  129. Horkova V, et al. 2020. Cell Reports. 30(5):1504-1514.e7.. PubMed
  130. Sinclair LV, et al. 2020. Immunometabolism. 2:e200029. PubMed
  131. Ku C, et al. 2015. Genes Dev. 29: 1930-1941. PubMed
  132. De Simone G, et al. 2021. Immunity. :. PubMed
  133. Coronel MM, et al. 2020. Sci Adv. 6:eaba5573. PubMed
  134. Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
  135. Biton M et al. 2018. Cell. 175(5):1307-1320 . PubMed
  136. Moore MJ et al. 2018. eLife. 7 pii: e33057. PubMed
  137. De Ponte Conti B, et al. 2021. Elife. 10:. PubMed
  138. Daneshmandi S, et al. 2021. Elife. 10:. PubMed
  139. Lindner J, et al. 2014. J Immunol. 192:4483. PubMed
  140. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  141. Zhai X, et al. 2021. Sci Adv. 7:eabk0490. PubMed
  142. Dionisio-Santos DA, et al. 2021. Front Neurosci. 15:758677. PubMed
  143. Evgin L, et al. 2020. Nat Commun. 2.671527778. PubMed
  144. Perry JL, et al. 2020. ACS Nano. 5.583333333. PubMed
  145. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  146. Qadir AS, et al. 2021. iScience. 24:103348. PubMed
  147. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  148. Moreau H, et al. 2015. Proc Natl Acad Sci U S A. 112: 12151-12156. PubMed
  149. Fernández-Orth J, et al. 2020. Eur J Immunol. . PubMed
  150. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  151. Li D, et al. 2020. Immunohorizons. 0.661805556. PubMed
  152. Mairhofer D, et al. 2015. J Invest Dermatol. 135: 2785-93. PubMed
  153. Rao R, et al. 2015. Toxicol Sci. 144:284. PubMed
  154. Porrello A, et al. 2018. Nat Commun. 9:1988. PubMed
  155. Li J, et al. 2021. Cell Rep. 37:110124. PubMed
  156. Fleskens V, et al. 2019. Cell Rep. 26:3600. PubMed
  157. Teng F, et al. 2021. Cell Rep. 37:110051. PubMed
  158. Kashiwakura Y, et al. 2020. Clinical & Experimental Immunology. 202(1):119-135. PubMed
  159. Steinmann S, et al. 2020. Sci Rep. 1.160416667. PubMed
  160. Kastenschmidt JM, et al. 2021. Cell Rep. 35:108997. PubMed
  161. Chen Y, et al. 2021. Braz J Med Biol Res. 54:e9570. PubMed
  162. Liu A, et al. 2022. Front Cell Infect Microbiol. 12:960208. PubMed
  163. Zaiss M, et al. 2015. Immunity. 43: 998-1010. PubMed
  164. Nabekura T, et al. 2020. Immunity. 96:52. PubMed
RRID
AB_312864 (BioLegend Cat. No. 102015)
AB_312865 (BioLegend Cat. No. 102016)

Antigen Details

Structure
Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132), 55 kD
Distribution

Activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells

Function
IL-2 receptor
Ligand/Receptor
IL-2
Cell Type
B cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References
  1. Taniguchi T, et al. 1993. Cell 73:5-8.
  2. Waldmann TA. 1991. J Biol Chem. 266:2681-4.
  3. Read S, et al. 2000. J Exp Med. 192:295-302.
  4. Lowenthal JW, et al. 1985. J Immunol. 135:3988-94.
Gene ID
16184 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org

Other Formats

View All CD25 Reagents Request Custom Conjugation
Description Clone Applications
PE/Cyanine7 anti-mouse CD25 PC61 FC
PE/Dazzle™ 594 anti-mouse CD25 PC61 FC
Brilliant Violet 711™ anti-mouse CD25 PC61 FC
Brilliant Violet 785™ anti-mouse CD25 PC61 FC
Alexa Fluor® 594 anti-mouse CD25 PC61 IHC-F
APC/Fire™ 750 anti-mouse CD25 PC61 FC
TotalSeq™-A0097 anti-mouse CD25 PC61 PG
KIRAVIA Blue 520™ anti-mouse CD25 PC61 FC
TotalSeq™-B0097 anti-mouse CD25 PC61 PG
TotalSeq™-C0097 anti-mouse CD25 PC61 PG
Spark NIR™ 685 anti-mouse CD25 Antibody PC61 FC
APC anti-mouse CD25 PC61 FC
Biotin anti-mouse CD25 PC61 FC
FITC anti-mouse CD25 PC61 FC
PE anti-mouse CD25 PC61 FC
PE/Cyanine5 anti-mouse CD25 PC61 FC
Purified anti-mouse CD25 PC61 FC,IHC-F,IP,Block,Depletion
Alexa Fluor® 488 anti-mouse CD25 PC61 FC
Alexa Fluor® 647 anti-mouse CD25 PC61 FC
Pacific Blue™ anti-mouse CD25 PC61 FC
Alexa Fluor® 700 anti-mouse CD25 PC61 FC
APC/Cyanine7 anti-mouse CD25 PC61 FC
PerCP/Cyanine5.5 anti-mouse CD25 PC61 FC
PerCP anti-mouse CD25 PC61 FC
Brilliant Violet 421™ anti-mouse CD25 PC61 FC
Brilliant Violet 605™ anti-mouse CD25 PC61 FC
Brilliant Violet 650™ anti-mouse CD25 PC61 FC
Ultra-LEAF™ Purified anti-mouse CD25 PC61 FC,IHC-F,IP,Block,Depletion
Brilliant Violet 510™ anti-mouse CD25 PC61 FC
PE/Fire™ 640 anti-mouse CD25 PC61 FC
Spark YG™ 581 anti-mouse CD25 PC61 FC
APC/Fire™ 810 anti-mouse CD25 PC61 FC
Brilliant Violet 750™ anti-mouse CD25 PC61 FC
PerCP/Fire™ 780 anti-mouse CD25 PC61 FC
PE/Fire™ 700 anti-mouse CD25 PC61 FC
Spark PLUS UV395™ anti-mouse CD25 PC61 FC
Spark Blue™ 574 anti-mouse CD25 (Flexi-Fluor™) PC61 FC
PerCP/Fire™ 806 anti-mouse CD25 Antibody PC61 FC
Spark PLUS B550™ anti-mouse CD25 PC61 FC
Spark YG™ 593 anti-mouse CD25 (Flexi-Fluor™) Antibody PC61 FC
Spark UV™ 387 anti-mouse CD25 (Flexi-Fluor™) PC61 FC
Spark PLUS V475™ anti-mouse CD25 Antibody PC61 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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