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PE/Cyanine7 anti-mouse CD4 Antibody PE/Cyanine7 anti-mouse CD4 Antibody

Pricing & Availability
Clone
GK1.5 (See other available formats)
Regulatory Status
RUO
Other Names
L3T4, T4
Isotype
Rat IgG2b, κ
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Product Citations
publications
GK1dot5_PECy7_030206
C57BL/6 mouse splenocytes were stained with CD4 (clone GK1.5) PE/Cyanine7 (filled histogram) or rat IgG2b, κ PE/Cyanine7 isotype control (open histogram).
  • GK1dot5_PECy7_030206
    C57BL/6 mouse splenocytes were stained with CD4 (clone GK1.5) PE/Cyanine7 (filled histogram) or rat IgG2b, κ PE/Cyanine7 isotype control (open histogram).
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
100422 100 µg DKK1278
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Description

CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosin kinase, lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CTL clone V4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: blocking of CD4+ T cell activation1,4,11, thymocyte costimulation3, in vitro and in vivo depletion2,5-8, blocking of egg-sperm cell adhesion1,4, immunohistochemical staining of acetone-fixed frozen sections9,10, immunoprecipitation1,2, and spatial biology (IBEX)12,13. The GK1.5 antibody is able to block CD4 mediated cell adhesion and T cell activation. Binding of GK1.5 antibody to CD4 T cells can be blocked by RM4-5 antibody, but not RM4-4 antibody. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100442) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Application References
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  2. Dialynas DP, et al. 1983. Immunol. Rev. 74:29. (IP, Deplete)
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  4. Godfrey DI, et al. 1994. J. Immunol. 152:4783. (Block)
  5. Gavett SH, et al. 1994. Am. J. Respir. Cell. Mol. Biol. 10:587. (Deplete)
  6. Schuyler M, et al. 1994. Am. J. Respir. Crit. Care Med. 149:1286. (Deplete)
  7. Ghobrial RR, et al. 1989. Clin. Immunol. Immunopathol. 52:486. (Deplete)
  8. Israelski DM, et al. 1989. J. Immunol. 142:954. (Deplete)
  9. Zheng B, et al. 1996. J. Exp. Med. 184:1083. (IHC)
  10. Frei K, et al. 1997. J. Exp. Med. 185:2177. (IHC)
  11. Felix NJ, et al. 2007. Nat. Immunol. 8:388. (Block)
  12. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  13. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
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  186. Iwamoto H et al. 2018. Cell metabolism. 28(1):104-117 . PubMed
  187. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  188. Espinosa-Cueto P, et al. 2017. PLoS One.. 10.1371/journal.pone.0182126. PubMed
  189. Garcia-Fabiani MB, et al. 2020. Methods Enzymol. 632:369. PubMed
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  191. Ferrere G, et al. 2021. JCI Insight. 6:. PubMed
  192. Di Martile M, et al. 2020. J Immunother Cancer. 8:. PubMed
  193. Zhou J, et al. 2021. Int J Med Sci. 18:3516. PubMed
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  195. Saleh MM, et al. 2019. Cell Host Microbe. 25:756. PubMed
  196. Coleby R, et al. 2021. Clin Exp Rheumatol. :39. PubMed
  197. Crespo ÂC, et al. 2020. Cell. 182(5):1125-1139. PubMed
  198. Periasamy S, et al. 2017. Nat Commun. 8:15564. PubMed
  199. Haertel E, et al. 2018. Eur J Immunol. 48:1001. PubMed
  200. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  201. Diao L, et al. 2022. iScience. 25:105511. PubMed
  202. del Rio ML, et al. 2021. Transl Res. Online ahead of print.. PubMed
  203. Fitzgerald AA, et al. 2021. J Immunother Cancer. 9:. PubMed
  204. Matsuoka S, et al. 2019. Haematologica. 105:226. PubMed
  205. Rolla S, et al. 2010. J Immunol. 184:6124. PubMed
  206. Kaufmann U et al. 2019. Cell metabolism. 29(5):1104-1118 . PubMed
  207. Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed
  208. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  209. Yoon Y, et al. 2021. Cancers (Basel). 13: . PubMed
  210. Ning X, et al. 2019. Mol Cell. 74:19. PubMed
  211. Dai B, et al. 2022. Theranostics. 12:7603. PubMed
  212. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  213. Haugh KA, et al. 2021. eLife. 0.416666666666667. PubMed
  214. Wang C, et al. 2021. Cell Stem Cell. . PubMed
  215. Lv H, et al. 2020. Cell Metabolism. 33(1):110-127.e5. PubMed
  216. Alissafi T, et al. 2020. Cell Metabolism. 32(4):591-604.e7. PubMed
  217. Ma YV, et al. 2021. MAbs. 13:2003281. PubMed
  218. Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed
  219. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  220. Wang H, et al. 2019. Nat Commun. 10:1898. PubMed
  221. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  222. Wei JL, et al. 2021. J Immunother Cancer. 9: . PubMed
  223. Wang F, et al. 2021. Cell Mol Gastroenterol Hepatol. 13:257. PubMed
  224. An J, et al. 2022. iScience. 25:103570. PubMed
  225. Ohmichi Y, et al. 2011. J Exp Med . 208:1015. PubMed
  226. Cha SE, et al. 2021. Oncoimmunology. 10:1899469. PubMed
  227. Tuganbaev T, et al. 2020. Cell. 182(6):1441-1459.e21. PubMed
  228. Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
  229. O'Brien S, Zhang M 2015. J Immunol. 195: 695-705. PubMed
  230. Wang W, et al. 2020. Immunity. 51(6):1102-1118.e7.. PubMed
  231. Akhter N, et al. 2016. J Biol Chem. 291: 23672 - 23680. PubMed
  232. Sido J, et al. 2015. J Leukoc Biol. 98: 435-447. PubMed
  233. Lee P, et al. 2014. J Immunol. 192:178. PubMed
  234. Becher J, et al. 2018. Dev Cell. 47:592. PubMed
  235. Chen G, et al. 2021. Sci Adv. 7:eabg5686. PubMed
  236. Elahi S, et al. 2020. Stem Cell Res. 43:101710. PubMed
  237. Bayik D, et al. 2020. Cancer Discov. 1.256944444. PubMed
  238. Pinho S, et al. 2022. Nat Cell Biol. 24:290. PubMed
  239. Yang J, et al. 2022. Microbiome. 10:149. PubMed
  240. Brandi P, et al. 2022. Cell Rep. 38:110184. PubMed
  241. Goldfarb Y, et al. 2021. J Exp Med. 218:. PubMed
  242. Momin N, et al. 2022. Nat Commun. 13:109. PubMed
  243. Luck H, et al. 2015. Cell Metab. 21 527 . PubMed
  244. Zhou G, et al. 2019. FASEB J. 33:11396. PubMed
  245. Faust HJ, et al. 2020. J Clin Invest. 130:5493. PubMed
  246. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  247. Vaena S, et al. 2021. Cell Reports. 35(5):109076. PubMed
  248. Lim CX, et al. 2020. Cell Rep. 3793:30. PubMed
  249. Shen M, et al. 2022. Nat Cancer. 3:60. PubMed
  250. Bullard BL, et al. 2022. NPJ Vaccines. 7:65. PubMed
  251. Kondo M, et al. 2016. J Immunol. 196: 563 - 572. PubMed
  252. XS R, et al. 2015. Diabetes. 64 90. PubMed
  253. Simula L et al. 2018. Cell reports. 25(11):3059-3073 . PubMed
  254. Chatterjee S et al. 2017. Cell metabolism. 27(1):85-100 . PubMed
  255. Li C, et al. 2021. Cell Metabolism. 33(8):1610-1623.e5. PubMed
  256. Otvos B, et al. 2021. Clin Cancer Res. 27:2038. PubMed
  257. De Ponte Conti B, et al. 2021. Elife. 10:. PubMed
  258. Dai R, et al. 2021. Front Immunol. 12:727046. PubMed
  259. Lebel MÈ, et al. 2020. Nat Commun. 3.051388889. PubMed
  260. Busse DC, et al. 2020. J Virol. 94:00:00. PubMed
  261. Koelwyn GJ, et al. 2020. Nat Med. 1452:26. PubMed
  262. Cui Z, et al. 2021. Cell Death Dis. 12:775. PubMed
  263. Kitajima Y, et al. 2022. Cells. 11:. PubMed
  264. Godwin MS, et al. 2019. JCI Insight. 4:e126070. PubMed
  265. Luck H, et al. 2019. Nat Commun. 10:3650. PubMed
  266. Spangenberg E, et al. 2019. Nat Commun. 10:3758. PubMed
  267. Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
  268. Zhou J, et al. 2022. Int J Oncol. 61: . PubMed
  269. Wang W, et al. 2021. J Am Heart Assoc. 10:e019142. PubMed
  270. Nakornpakdee Y, et al. 2018. Asian Pac J Allergy Immunol. 36:265. PubMed
  271. Pardo E, et al. 2017. PLoS One. 12(6):e0177472. PubMed
  272. Draijer C, et al. 2018. Sci Rep. 8:5105. PubMed
  273. Isvoranu G, et al. 2019. Oncol Lett. 17:4197. PubMed
  274. Li C, et al. 2018. Cell. 174:285. PubMed
  275. Wang W, et al. 2021. STAR Protocols. 2(3):100756. PubMed
  276. Schönberger K, et al. 2022. STAR Protoc. 3:101408. PubMed
  277. Wang J, et al. 2022. Clin Transl Med. 12:e718. PubMed
  278. Kojima H, et al. 2021. Scand J Immunol. 93:e13020. PubMed
  279. Sanders K, et al. 2015. Cancer Immunol Res. 3: 891-901. PubMed
  280. Chen M, et al. 2019. Cell Stem Cell. 25:501. PubMed
  281. Li N, et al. 2022. Nat Commun. 13:7281. PubMed
  282. Godwin MS, et al. 2021. Am J Physiol Lung Cell Mol Physiol. 320:L393. PubMed
  283. Prüss H, et al. 2017. Nat Neurosci. 10.1038/nn.4643. PubMed
  284. Kimura S, et al. 2020. Am J Transplant. 20:977. PubMed
  285. Kelley WJ, et al. 2022. JCI Insight. 7: . PubMed
  286. Kim S, et al. 2022. Cancers (Basel). 14:. PubMed
  287. Ambrosino E, et al. 2006. Cancer Res . 66:7734. PubMed
  288. Nagamata S, et al. 2019. Sci Rep. 9:3911. PubMed
  289. Ukidve A, et al. 2020. Proc Natl Acad Sci U S A. 117:17727. PubMed
  290. Nagatake T, et al. 2021. Int Immunol. 33:171. PubMed
  291. RL M, et al. 2015. Proc Natl Acad Sci U S A. 112:6506-6514. PubMed
  292. Angela M, et al. 2016. Nat Commun. 7:13683. PubMed
  293. Kim YS, et al. 2018. Front Immunol. 9:1093. PubMed
  294. Le TM et al. 2018. Journal of neurochemistry. 145(2):139-153 . PubMed
  295. Sadtler K, et al. 2017. Tissue Eng Part A. 23:1044. PubMed
  296. Miao Y et al. 2019. Cell. 177(5):1172-1186 . PubMed
  297. Lerbs T, et al. 2020. JCI Insight. 5:00. PubMed
  298. Schäfer AL, et al. 2021. Front Immunol. 12:696810. PubMed
  299. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  300. Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
  301. Simula L, et al. 2022. Mol Oncol. 16:188. PubMed
  302. Qi S, et al. 2020. Theranostics. 10:1814. PubMed
  303. Groves HT, et al. 2020. MBio. 11:e03236-19. PubMed
  304. Yu Z, et al. 2021. Biomed Res Int. 2021:9958745. PubMed
  305. Amend A, et al. 2021. Int J Mol Sci. 22:. PubMed
  306. Song X, et al. 2022. Transl Oncol. 15:101306. PubMed
RRID
AB_312707 (BioLegend Cat. No. 100422)

Antigen Details

Structure
Ig superfamily, 55 kD
Distribution

Majority of thymocytes, T cell subset

Function
TCR co-receptor, T cell activation
Ligand/Receptor
MHC class II molecule
Cell Type
Dendritic cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.

Gene ID
12504 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.

Other Formats

View All CD4 Reagents Request Custom Conjugation
Description Clone Applications
APC/Cyanine7 anti-mouse CD4 GK1.5 FC
Brilliant Violet 711™ anti-mouse CD4 GK1.5 FC
Brilliant Violet 510™ anti-mouse CD4 GK1.5 FC
Brilliant Violet 605™ anti-mouse CD4 GK1.5 FC
Brilliant Violet 785™ anti-mouse CD4 GK1.5 FC
PE/Dazzle™ 594 anti-mouse CD4 GK1.5 FC
APC/Fire™ 750 anti-mouse CD4 GK1.5 FC
GoInVivo™ Purified anti-mouse CD4 GK1.5 FC
Brilliant Violet 750™ anti-mouse CD4 GK1.5 FC
Brilliant Violet 650™ anti-mouse CD4 GK1.5 FC
Spark Blue™ 550 anti-mouse CD4 GK1.5 FC
Spark NIR™ 685 anti-mouse CD4 GK1.5 FC
KIRAVIA Blue 520™ anti-mouse CD4 GK1.5 FC
PE/Fire™ 640 anti-mouse CD4 GK1.5 FC
APC/Fire™ 810 anti-mouse CD4 GK1.5 FC
PE/Fire™ 700 anti-mouse CD4 GK1.5 FC
APC anti-mouse CD4 GK1.5 FC
Biotin anti-mouse CD4 GK1.5 FC,IHC-F,ICC
FITC anti-mouse CD4 GK1.5 FC
PE anti-mouse CD4 GK1.5 FC
PE/Cyanine5 anti-mouse CD4 GK1.5 FC
Purified anti-mouse CD4 GK1.5 FC,IHC-F,ICC,IP,Costim,Block,Depletion
Alexa Fluor® 647 anti-mouse CD4 GK1.5 FC,IHC-F
Alexa Fluor® 488 anti-mouse CD4 GK1.5 FC,IHC-F
Pacific Blue™ anti-mouse CD4 GK1.5 FC
Alexa Fluor® 700 anti-mouse CD4 GK1.5 FC
PerCP anti-mouse CD4 GK1.5 FC
PerCP/Cyanine5.5 anti-mouse CD4 GK1.5 FC
Brilliant Violet 421™ anti-mouse CD4 GK1.5 FC,ICC,IHC-F
Ultra-LEAF™ Purified anti-mouse CD4 GK1.5 FC,Block,Costim,Depletion,IHC,IP
Alexa Fluor® 594 anti-mouse CD4 GK1.5 IHC-F,FC,SB
Spark Violet™ 538 anti-mouse CD4 GK1.5 FC
Spark YG™ 593 anti-mouse CD4 GK1.5 FC
Spark Blue™ 574 anti-mouse CD4 Antibody GK1.5 FC
Spark UV™ 387 anti-mouse CD4 GK1.5 FC
Spark Blue™ 515 anti-mouse CD4 GK1.5 FC
Spark PLUS UV395™ anti-mouse CD4 GK1.5 FC
Spark Red™ 718 anti-mouse CD4 (Flexi-Fluor™) GK1.5 FC
PerCP/Fire™ 806 anti-mouse CD4 Antibody GK1.5 FC
PerCP/Fire™ 780 anti-mouse CD4 Antibody GK1.5 FC
Spark YG™ 581 anti-mouse CD4 (Flexi-Fluor™) GK1.5 FC
StarBright UltraViolet 795 anti-mouse CD4 GK1.5 FC
Spark PLUS V475™ anti-mouse CD4 GK1.5 FC
Spark PLUS B488™ anti-mouse CD4 GK1.5 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Pricing & Availability
Clone
GK1.5 (See other available formats)
Regulatory Status
RUO
Other Names
L3T4, T4
Isotype
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
GK1dot5_PECy7_030206
C57BL/6 mouse splenocytes were stained with CD4 (clone GK1.5) PE/Cyanine7 (filled histogram) or rat IgG2b, κ PE/Cyanine7 isotype control (open histogram).
  • GK1dot5_PECy7_030206
    C57BL/6 mouse splenocytes were stained with CD4 (clone GK1.5) PE/Cyanine7 (filled histogram) or rat IgG2b, κ PE/Cyanine7 isotype control (open histogram).
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
100422 100 µg DKK1278
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Description

CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosin kinase, lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CTL clone V4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: blocking of CD4+ T cell activation1,4,11, thymocyte costimulation3, in vitro and in vivo depletion2,5-8, blocking of egg-sperm cell adhesion1,4, immunohistochemical staining of acetone-fixed frozen sections9,10, immunoprecipitation1,2, and spatial biology (IBEX)12,13. The GK1.5 antibody is able to block CD4 mediated cell adhesion and T cell activation. Binding of GK1.5 antibody to CD4 T cells can be blocked by RM4-5 antibody, but not RM4-4 antibody. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100442) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Application References
  1. Dialynas DP, et al. 1983. J. Immunol. 131:2445. (Block, IP)
  2. Dialynas DP, et al. 1983. Immunol. Rev. 74:29. (IP, Deplete)
  3. Wu L, et al. 1991. J. Exp. Med. 174:1617. (Costim)
  4. Godfrey DI, et al. 1994. J. Immunol. 152:4783. (Block)
  5. Gavett SH, et al. 1994. Am. J. Respir. Cell. Mol. Biol. 10:587. (Deplete)
  6. Schuyler M, et al. 1994. Am. J. Respir. Crit. Care Med. 149:1286. (Deplete)
  7. Ghobrial RR, et al. 1989. Clin. Immunol. Immunopathol. 52:486. (Deplete)
  8. Israelski DM, et al. 1989. J. Immunol. 142:954. (Deplete)
  9. Zheng B, et al. 1996. J. Exp. Med. 184:1083. (IHC)
  10. Frei K, et al. 1997. J. Exp. Med. 185:2177. (IHC)
  11. Felix NJ, et al. 2007. Nat. Immunol. 8:388. (Block)
  12. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  13. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
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  6. Xiong T, et al. 2022. Cancer Sci. 113:1955. PubMed
  7. Chakraborty P, et al. 2022. Cancer Res. 82:1969. PubMed
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  13. Zhu J, et al. 2022. Nat Commun. 13:7466. PubMed
  14. Roy S, et al. 2022. Am J Cancer Res. 12:4977. PubMed
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  140. Kim YS, et al. 2018. Front Immunol. 9:1093. PubMed
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  142. Sadtler K, et al. 2017. Tissue Eng Part A. 23:1044. PubMed
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  144. Lerbs T, et al. 2020. JCI Insight. 5:00. PubMed
  145. Schäfer AL, et al. 2021. Front Immunol. 12:696810. PubMed
  146. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  147. Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
  148. Simula L, et al. 2022. Mol Oncol. 16:188. PubMed
  149. Qi S, et al. 2020. Theranostics. 10:1814. PubMed
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  151. Yu Z, et al. 2021. Biomed Res Int. 2021:9958745. PubMed
  152. Amend A, et al. 2021. Int J Mol Sci. 22:. PubMed
  153. Song X, et al. 2022. Transl Oncol. 15:101306. PubMed
  154. Wang X, et al. 2019. Cell Res. 29:787. PubMed
  155. Wu J et al. 2017. Immunity. 47(6):1114-1128 . PubMed
  156. Perruzza L, et al. 2019. Sci Rep. 9:9315. PubMed
  157. Collin R, et al. 2020. J Immunol. 205:133. PubMed
  158. Alam A, et al. 2022. Cancer Cell. 40:153. PubMed
  159. Xiong T, et al. 2022. Cancer Sci. 113:1955. PubMed
  160. Chakraborty P, et al. 2022. Cancer Res. 82:1969. PubMed
  161. Zhao Z, et al. 2022. Adv Sci (Weinh). 9:e2201293. PubMed
  162. del Rio ML, et al. 2022. Front Immunol. 13:887348. PubMed
  163. Lin G, et al. 2022. Int J Immunopathol Pharmacol. 36:3946320221117933. PubMed
  164. Kang IH, et al. 2022. iScience. 25:104818. PubMed
  165. Mansouri S, et al. 2022. J Immunol. 209:2114. PubMed
  166. Zhu J, et al. 2022. Nat Commun. 13:7466. PubMed
  167. Roy S, et al. 2022. Am J Cancer Res. 12:4977. PubMed
  168. Kraemer AN, et al. 2022. Front Immunol. 13:933191. PubMed
  169. Kulkarni R, et al. 2023. Life Sci Alliance. 6: . PubMed
  170. Kasuya T, et al. 2023. Sci Rep. 13:1653. PubMed
  171. Zhao K, et al. 2023. Front Immunol. 14:1101769. PubMed
  172. Novaj A, et al. 2023. Int J Mol Sci. 24: . PubMed
  173. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  174. Kodali S, et al. 2022. J Immunol. 208:1085. PubMed
  175. Meacham CE, et al. 2022. Nat Cell Biol. 24:697. PubMed
  176. Sanmarco LM, et al. 2022. Nature. 611:801. PubMed
  177. Wang X, et al. 2023. J Immunother Cancer. 11:. PubMed
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RRID
AB_312707 (BioLegend Cat. No. 100422)

Antigen Details

Structure
Ig superfamily, 55 kD
Distribution

Majority of thymocytes, T cell subset

Function
TCR co-receptor, T cell activation
Ligand/Receptor
MHC class II molecule
Cell Type
Dendritic cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.

Gene ID
12504 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.

Other Formats

View All CD4 Reagents Request Custom Conjugation
Description Clone Applications
APC/Cyanine7 anti-mouse CD4 GK1.5 FC
Brilliant Violet 711™ anti-mouse CD4 GK1.5 FC
Brilliant Violet 510™ anti-mouse CD4 GK1.5 FC
Brilliant Violet 605™ anti-mouse CD4 GK1.5 FC
Brilliant Violet 785™ anti-mouse CD4 GK1.5 FC
PE/Dazzle™ 594 anti-mouse CD4 GK1.5 FC
APC/Fire™ 750 anti-mouse CD4 GK1.5 FC
GoInVivo™ Purified anti-mouse CD4 GK1.5 FC
Brilliant Violet 750™ anti-mouse CD4 GK1.5 FC
Brilliant Violet 650™ anti-mouse CD4 GK1.5 FC
Spark Blue™ 550 anti-mouse CD4 GK1.5 FC
Spark NIR™ 685 anti-mouse CD4 GK1.5 FC
KIRAVIA Blue 520™ anti-mouse CD4 GK1.5 FC
PE/Fire™ 640 anti-mouse CD4 GK1.5 FC
APC/Fire™ 810 anti-mouse CD4 GK1.5 FC
PE/Fire™ 700 anti-mouse CD4 GK1.5 FC
APC anti-mouse CD4 GK1.5 FC
Biotin anti-mouse CD4 GK1.5 FC,IHC-F,ICC
FITC anti-mouse CD4 GK1.5 FC
PE anti-mouse CD4 GK1.5 FC
PE/Cyanine5 anti-mouse CD4 GK1.5 FC
Purified anti-mouse CD4 GK1.5 FC,IHC-F,ICC,IP,Costim,Block,Depletion
Alexa Fluor® 647 anti-mouse CD4 GK1.5 FC,IHC-F
Alexa Fluor® 488 anti-mouse CD4 GK1.5 FC,IHC-F
Pacific Blue™ anti-mouse CD4 GK1.5 FC
Alexa Fluor® 700 anti-mouse CD4 GK1.5 FC
PerCP anti-mouse CD4 GK1.5 FC
PerCP/Cyanine5.5 anti-mouse CD4 GK1.5 FC
Brilliant Violet 421™ anti-mouse CD4 GK1.5 FC,ICC,IHC-F
Ultra-LEAF™ Purified anti-mouse CD4 GK1.5 FC,Block,Costim,Depletion,IHC,IP
Alexa Fluor® 594 anti-mouse CD4 GK1.5 IHC-F,FC,SB
Spark Violet™ 538 anti-mouse CD4 GK1.5 FC
Spark YG™ 593 anti-mouse CD4 GK1.5 FC
Spark Blue™ 574 anti-mouse CD4 Antibody GK1.5 FC
Spark UV™ 387 anti-mouse CD4 GK1.5 FC
Spark Blue™ 515 anti-mouse CD4 GK1.5 FC
Spark PLUS UV395™ anti-mouse CD4 GK1.5 FC
Spark Red™ 718 anti-mouse CD4 (Flexi-Fluor™) GK1.5 FC
PerCP/Fire™ 806 anti-mouse CD4 Antibody GK1.5 FC
PerCP/Fire™ 780 anti-mouse CD4 Antibody GK1.5 FC
Spark YG™ 581 anti-mouse CD4 (Flexi-Fluor™) GK1.5 FC
StarBright UltraViolet 795 anti-mouse CD4 GK1.5 FC
Spark PLUS V475™ anti-mouse CD4 GK1.5 FC
Spark PLUS B488™ anti-mouse CD4 GK1.5 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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