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StarBright UltraViolet 795 anti-mouse CD45.1 Antibody StarBright UltraViolet 795 anti-mouse CD45.1 Antibody

Pricing & Availability
Clone
A20 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5.1, LCA
Isotype
Mouse (A.SW) IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
A20_SBUV795_CD451_FC_1_090225
SJL mouse splenocytes were stained with anti-mouse CD3ε (clone 145-2C11) APC and anti-mouse CD45.1 (clone A20) StarBright UltraViolet 795 (left) or stained with anti-mouse CD3ε (clone 145-2C11) APC only (right).
  • A20_SBUV795_CD451_FC_1_090225
    SJL mouse splenocytes were stained with anti-mouse CD3ε (clone 145-2C11) APC and anti-mouse CD45.1 (clone A20) StarBright UltraViolet 795 (left) or stained with anti-mouse CD3ε (clone 145-2C11) APC only (right).
Compare all formats
Cat # Size Price Quantity Check Availability Save
110763 150 μg DKK1788
Check Availability


Need larger quantities of this item?
Request Bulk Quote

This StarBright conjugate is available for purchase as part of a multicolor flow cytometry panel. To order, add compatible products to your shopping cart, or use our Panel Selector tool to build your ideal panel. For ordering assistance or to request a sample, contact your local field representative.

This product is also available for direct purchase within a StarBright Basic Panel, which combines a minimal set of pre-selected compatible reagents.

Description

CD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (e.g., RIII, SJL/J, STS/A, DA). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in mice that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell types. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SJL mouse thymocytes and splenocytes
Formulation
Phosphate-buffered saline, containing 0.09% sodium azide, 0.05% Tween 20, 0.1% PEG 3350, 0.1% Pluronic F68, and 1% BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with StarBright UltraViolet 795 under optimal conditions.
Concentration
0.6 mg/mL (the concentration relates to the dye component of the conjugate)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* StarBright UltraViolet 795 has a maximum excitation of 340 nm and a maximum emission of 792 nm.


StarBright dyes are powered by Bio-Rad®. STARBRIGHT is a trademark of Bio-Rad Laboratories, Inc.
Excitation Laser
Ultraviolet Laser (355 nm)
Application Notes

The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)).

Application References
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  4. Suzuki K, et al. 2000. Immunity 13:691. (IHC-F)
  5. Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC-F)
  6. Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC-F)
  7. Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC-F)
  8. Pascal V, et al. 2007. J. Immunol. 179:1751. (FC)
  9. Mende I, et al. 2006. Blood 107:1383. (IHC-F, FC)
  10. Phan TG, et al. 2007. Nature Immunol. 8:992. (FC)
  11. Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed
  12. Pascal V, et al.2007. J. Immunol. 179:1751. PubMed
  13. Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed
  14. Takada K, et al. 2009. J. Exp Med. 206:2253. PubMed
  15. Beamer CA, et al. 2007. Am. J. Respir. Cell. Mol. Biol. 37:729. (FC) PubMed
  16. Li LX, et al. 2010. J. Immunol. 184:1728. PubMed
  17. Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed
  18. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  19. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.1 on UniProt.org

Related FAQs

What is a “StarBright panel”?

A StarBright panel is a multiplex panel that includes at least one StarBright conjugate.

Will my StarBright panel be provided as a cocktail?

StarBright products purchased as part of a panel or StarBright Basic Panel will be provided in individual vials. To order premade cocktails, please contact our Custom Solutions Team.

Are StarBright GMP/ASR products available?

StarBright ASR and GMP products are coming soon.

Are StarBright custom products available?

Yes, please contact our Custom Solutions Team.

Will StarBright isotype controls be offered?

We are not planning to offer StarBright isotype controls at this time. Fluorescence Minus One (FMO) controls should be used to define gating boundaries.

What amount of a StarBright conjugate should I use?

Antibody conjugates should be titrated for optimal performance in each application. To directly compare performance with a traditional or polymer-conjugated antibody, we recommend using the same volume of each product for analysis. Due to differences in the quantification of product mass, StarBright Dyes are prepared in equivalent volumes.

Why shouldn’t I compare the mass (μg) of a StarBright conjugate to a traditional or polymer conjugate?

For StarBright conjugates, the reported mass refers to the mass of the dye component. For traditional conjugates, the reported mass refers to the mass of the antibody component. This is because traditional conjugates have multiple dye molecules attached to one antibody, while StarBright conjugates have multiple antibodies attached to a single StarBright Dye.

Other Formats

View All CD45.1 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse CD45.1 A20 FC,IP,Block,IHC-F
PE anti-mouse CD45.1 A20 FC
FITC anti-mouse CD45.1 A20 FC
Biotin anti-mouse CD45.1 A20 FC
Purified anti-mouse CD45.1 (Maxpar® Ready) A20 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD45.1 A20 FC
Alexa Fluor® 594 anti-mouse CD45.1 A20 IHC-F
APC/Fire™ 750 anti-mouse CD45.1 A20 FC
TotalSeq™-A0178 anti-mouse CD45.1 A20 PG
TotalSeq™-B0178 anti-mouse CD45.1 A20 PG
TotalSeq™-C0178 anti-mouse CD45.1 A20 PG
APC/Cyanine7 anti-mouse CD45.1 A20 FC
APC anti-mouse CD45.1 A20 FC
Alexa Fluor® 488 anti-mouse CD45.1 A20 FC
Alexa Fluor® 647 anti-mouse CD45.1 A20 FC
Pacific Blue™ anti-mouse CD45.1 A20 FC
Alexa Fluor® 700 anti-mouse CD45.1 A20 FC
Brilliant Violet 650™ anti-mouse CD45.1 A20 FC
PerCP anti-mouse CD45.1 A20 FC
PerCP/Cyanine5.5 anti-mouse CD45.1 A20 FC
PE/Cyanine7 anti-mouse CD45.1 A20 FC
Brilliant Violet 421™ anti-mouse CD45.1 A20 FC
Brilliant Violet 570™ anti-mouse CD45.1 A20 FC
Brilliant Violet 605™ anti-mouse CD45.1 A20 FC
Brilliant Violet 711™ anti-mouse CD45.1 A20 FC
Brilliant Violet 510™ anti-mouse CD45.1 A20 FC
Brilliant Violet 785™ anti-mouse CD45.1 A20 FC
Spark UV™ 387 anti-mouse CD45.1 A20 FC
Spark Blue™ 550 anti-mouse CD45.1 (Flexi-Fluor™) A20 FC
Spark Red™ 718 anti-mouse CD45.1 (Flexi-Fluor™) A20 FC
Spark Blue™ 574 anti-mouse CD45.1 (Flexi-Fluor™) A20 FC
Spark YG™ 581 anti-mouse CD45.1 (Flexi-Fluor™) A20 FC
Spark YG™ 593 anti-mouse CD45.1 (Flexi-Fluor™) Antibody A20 FC
Spark NIR™ 685 anti-mouse CD45.1 (Flexi-Fluor™) Antibody A20 FC
StarBright UltraViolet 795 anti-mouse CD45.1 A20 FC
Go To Top Version: 1    Revision Date: 07/23/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Pricing & Availability
Clone
A20 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5.1, LCA
Isotype
Mouse (A.SW) IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
A20_SBUV795_CD451_FC_1_090225
SJL mouse splenocytes were stained with anti-mouse CD3ε (clone 145-2C11) APC and anti-mouse CD45.1 (clone A20) StarBright UltraViolet 795 (left) or stained with anti-mouse CD3ε (clone 145-2C11) APC only (right).
  • A20_SBUV795_CD451_FC_1_090225
    SJL mouse splenocytes were stained with anti-mouse CD3ε (clone 145-2C11) APC and anti-mouse CD45.1 (clone A20) StarBright UltraViolet 795 (left) or stained with anti-mouse CD3ε (clone 145-2C11) APC only (right).
Compare all formats
Cat # Size Price Quantity Check Availability Save
110763 150 μg DKK1788
Check Availability


Need larger quantities of this item?
Request Bulk Quote

This StarBright conjugate is available for purchase as part of a multicolor flow cytometry panel. To order, add compatible products to your shopping cart, or use our Panel Selector tool to build your ideal panel. For ordering assistance or to request a sample, contact your local field representative.

This product is also available for direct purchase within a StarBright Basic Panel, which combines a minimal set of pre-selected compatible reagents.

Description

CD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (e.g., RIII, SJL/J, STS/A, DA). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in mice that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell types. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SJL mouse thymocytes and splenocytes
Formulation
Phosphate-buffered saline, containing 0.09% sodium azide, 0.05% Tween 20, 0.1% PEG 3350, 0.1% Pluronic F68, and 1% BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with StarBright UltraViolet 795 under optimal conditions.
Concentration
0.6 mg/mL (the concentration relates to the dye component of the conjugate)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* StarBright UltraViolet 795 has a maximum excitation of 340 nm and a maximum emission of 792 nm.


StarBright dyes are powered by Bio-Rad®. STARBRIGHT is a trademark of Bio-Rad Laboratories, Inc.
Excitation Laser
Ultraviolet Laser (355 nm)
Application Notes

The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)).

Application References
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  4. Suzuki K, et al. 2000. Immunity 13:691. (IHC-F)
  5. Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC-F)
  6. Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC-F)
  7. Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC-F)
  8. Pascal V, et al. 2007. J. Immunol. 179:1751. (FC)
  9. Mende I, et al. 2006. Blood 107:1383. (IHC-F, FC)
  10. Phan TG, et al. 2007. Nature Immunol. 8:992. (FC)
  11. Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed
  12. Pascal V, et al.2007. J. Immunol. 179:1751. PubMed
  13. Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed
  14. Takada K, et al. 2009. J. Exp Med. 206:2253. PubMed
  15. Beamer CA, et al. 2007. Am. J. Respir. Cell. Mol. Biol. 37:729. (FC) PubMed
  16. Li LX, et al. 2010. J. Immunol. 184:1728. PubMed
  17. Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed
  18. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  19. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.1 on UniProt.org

Related FAQs

What is a “StarBright panel”?

A StarBright panel is a multiplex panel that includes at least one StarBright conjugate.

Will my StarBright panel be provided as a cocktail?

StarBright products purchased as part of a panel or StarBright Basic Panel will be provided in individual vials. To order premade cocktails, please contact our Custom Solutions Team.

Are StarBright GMP/ASR products available?

StarBright ASR and GMP products are coming soon.

Are StarBright custom products available?

Yes, please contact our Custom Solutions Team.

Will StarBright isotype controls be offered?

We are not planning to offer StarBright isotype controls at this time. Fluorescence Minus One (FMO) controls should be used to define gating boundaries.

What amount of a StarBright conjugate should I use?

Antibody conjugates should be titrated for optimal performance in each application. To directly compare performance with a traditional or polymer-conjugated antibody, we recommend using the same volume of each product for analysis. Due to differences in the quantification of product mass, StarBright Dyes are prepared in equivalent volumes.

Why shouldn’t I compare the mass (μg) of a StarBright conjugate to a traditional or polymer conjugate?

For StarBright conjugates, the reported mass refers to the mass of the dye component. For traditional conjugates, the reported mass refers to the mass of the antibody component. This is because traditional conjugates have multiple dye molecules attached to one antibody, while StarBright conjugates have multiple antibodies attached to a single StarBright Dye.

Other Formats

View All CD45.1 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse CD45.1 A20 FC,IP,Block,IHC-F
PE anti-mouse CD45.1 A20 FC
FITC anti-mouse CD45.1 A20 FC
Biotin anti-mouse CD45.1 A20 FC
Purified anti-mouse CD45.1 (Maxpar® Ready) A20 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD45.1 A20 FC
Alexa Fluor® 594 anti-mouse CD45.1 A20 IHC-F
APC/Fire™ 750 anti-mouse CD45.1 A20 FC
TotalSeq™-A0178 anti-mouse CD45.1 A20 PG
TotalSeq™-B0178 anti-mouse CD45.1 A20 PG
TotalSeq™-C0178 anti-mouse CD45.1 A20 PG
APC/Cyanine7 anti-mouse CD45.1 A20 FC
APC anti-mouse CD45.1 A20 FC
Alexa Fluor® 488 anti-mouse CD45.1 A20 FC
Alexa Fluor® 647 anti-mouse CD45.1 A20 FC
Pacific Blue™ anti-mouse CD45.1 A20 FC
Alexa Fluor® 700 anti-mouse CD45.1 A20 FC
Brilliant Violet 650™ anti-mouse CD45.1 A20 FC
PerCP anti-mouse CD45.1 A20 FC
PerCP/Cyanine5.5 anti-mouse CD45.1 A20 FC
PE/Cyanine7 anti-mouse CD45.1 A20 FC
Brilliant Violet 421™ anti-mouse CD45.1 A20 FC
Brilliant Violet 570™ anti-mouse CD45.1 A20 FC
Brilliant Violet 605™ anti-mouse CD45.1 A20 FC
Brilliant Violet 711™ anti-mouse CD45.1 A20 FC
Brilliant Violet 510™ anti-mouse CD45.1 A20 FC
Brilliant Violet 785™ anti-mouse CD45.1 A20 FC
Spark UV™ 387 anti-mouse CD45.1 A20 FC
Spark Blue™ 550 anti-mouse CD45.1 (Flexi-Fluor™) A20 FC
Spark Red™ 718 anti-mouse CD45.1 (Flexi-Fluor™) A20 FC
Spark Blue™ 574 anti-mouse CD45.1 (Flexi-Fluor™) A20 FC
Spark YG™ 581 anti-mouse CD45.1 (Flexi-Fluor™) A20 FC
Spark YG™ 593 anti-mouse CD45.1 (Flexi-Fluor™) Antibody A20 FC
Spark NIR™ 685 anti-mouse CD45.1 (Flexi-Fluor™) Antibody A20 FC
StarBright UltraViolet 795 anti-mouse CD45.1 A20 FC
Go To Top Version: 1    Revision Date: 07/23/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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