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True-Nuclear™ Transcription Factor Buffer Set True-Nuclear™ Transcription Factor Buffer Set

Pricing & Availability
Regulatory Status
RUO
Other Names
Transcription Factor Fixation/Permeabilization Buffer Set
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Product Citations
publications
Transcription_Factor_F_P_Buffer_1_ICFC_012915
Human peripheral blood lymphocytes were surface stained with CD3 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with T-bet (clone 4B10) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • Transcription_Factor_F_P_Buffer_1_ICFC_012915
    Human peripheral blood lymphocytes were surface stained with CD3 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with T-bet (clone 4B10) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • Transcription_Factor_F_P_Buffer_2_ICFC_012915
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424401 120 tests DKK981
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Description

BioLegend's True-Nuclear™ Transcription Factor Buffer Set has been specially formulated for intracellular staining with minimum effect on the surface fluorochrome staining.

See the advantages of True-Nuclear™ Transcription Factor Buffer Set in this brief webinar: biolegend.com/true_nuclear_webinar.


 

Caution: The True-Nuclear™ Transcription Factor Buffer Set contains paraformaldehyde, which is toxic and mutagenic. Please handle with caution. Wear gloves, lab coats, and necessary protection to avoid direct contact. 

Product Details
Technical Data Sheet (pdf)

Product Details

Storage & Handling
Store between 2°C and 8°C. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Prepare fresh Transcription Factor Fix working solution by diluting the 4X Fix Concentrate (1 part) with the Fix Diluent (3 parts). 1 mL of the 1X Fix working solution is needed for each tube.

Prepare a 1X working solution of the Perm Buffer by diluting the 10X Perm Buffer with distilled water. 6.5 mL of 1X Perm Buffer is needed for each sample of tube.

NOTE: The 10X Perm Buffer may have crystallization or precipitation when it is stored at 2-8°C; however, this is normal and does not affect the buffer's performance. If there is a heavy precipitation observed after dilution to 1X working solution, it can be filtered to clarify the solution.

Application Notes

Buffer Set Contents:

  • True-Nuclear™ 4X Fix Concentrate (30 mL)
  • True-Nuclear™ Fix Diluent (100 mL)
  • True-Nuclear™ 10X Perm Buffer (100 mL)

 

Additional Product Notes

View more applications data using this product to stain Veri-Cells™ lyophilized control cells, to detect transcription factors, and to study TIGIT in our Scientific Poster Library.

Product Citations
  1. Ogawa T, et al. 2020. J Immunol. 205:907. PubMed
  2. Ajith A, et al. 2021. Front Immunol. 12:687715. PubMed
  3. Douglass SM, et al. 2021. Cancer Res. 81:658. PubMed
  4. Capelle CM, et al. 2022. Immunology. 165:428. PubMed
  5. Ellis GI, et al. 2022. Cell Rep Med. 3:100614. PubMed
  6. Grant NL, et al. 2022. Cell Rep. 39:110826. PubMed
  7. Hu M, et al. 2022. Int J Mol Sci. 23: . PubMed
  8. Jou E, et al. 2022. Sci Immunol. 7:eabn0175. PubMed
  9. Yu H, et al. 2022. Hematol Oncol. 40:894. PubMed
  10. Ogishi M, et al. 2023. J Exp Med. 220: . PubMed
  11. Isildar B, et al. 2022. Inflamm Regen. 42:55. PubMed
  12. Noman MZ, et al. 2022. Front Immunol. 13:980704. PubMed
  13. He Y, et al. 2022. Front Immunol. 13:986202. PubMed
  14. Ishii T, et al. 2022. Cell Death Discov. 8:480. PubMed
  15. Chen X, et al. 2022. J Extracell Vesicles. 11:e12279. PubMed
  16. Weng S, et al. 2022. Front Immunol. 13:1025931. PubMed
  17. Landshammer A, et al. 2023. Elife. 12: . PubMed
  18. Vyas M, et al. 2023. Front Immunol. 13:1098445. PubMed
  19. Wang L, et al. 2023. Oxid Med Cell Longev. 2023:6480848. PubMed
  20. Rustagi Y, et al. 2022. Diabetes. 71:1149. PubMed
  21. Zander R, et al. 2022. Immunity. 55:475. PubMed
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  23. Shen M, et al. 2022. Adv Sci (Weinh). 9:e2203523. PubMed
  24. Cook ME, et al. 2022. Sci Immunol. 7:eabo0981. PubMed
  25. Whyte CE, et al. 2022. Curr Protoc. 2:e589. PubMed
  26. Lao L, et al. 2023. Cancer Immunol Res. 11:320. PubMed
  27. Li Y, et al. 2023. Adv Mater. 35:e2208923. PubMed
  28. Schwarz A, et al. 2023. Front Immunol. 14:1038689. PubMed
  29. Kozlovski S, et al. 2023. Front Immunol. 13:1041552. PubMed
  30. Yi X, et al. 2023. Signal Transduct Target Ther. 8:107. PubMed
  31. Martins Nascentes Melo L, et al. 2023. J Immunother Cancer. 11:. PubMed
  32. McKenzie C, et al. 2023. Mol Ther Nucleic Acids. 32:603. PubMed
  33. Wu C, et al. 2023. Cancer Metab. 11:7. PubMed
  34. Adu-Berchie K, et al. 2023. Nat Commun. 14:3546. PubMed
  35. Wang L, et al. 2019. Front Pharmacol. 1.342361111. PubMed
  36. Manso BA, et al. 2021. STAR Protoc. 2:100485. PubMed
  37. Wu HJ, et al. 2021. Nat Commun. 12:4897. PubMed
  38. Zeng N, et al. 2022. EMBO Rep. 23:e53302. PubMed
  39. Bujanover N, et al. 2021. Stem Cell Reports. 16:1884. PubMed
  40. Kim D, et al. 2021. Nanomicro Lett. 13:31. PubMed
  41. Zhang M, et al. 2020. Nat Commun. 4.707638889. PubMed
  42. Li X, et al. 2022. Nat Commun. 13:2794. PubMed
  43. Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
  44. Hu HJ, et al. 2020. Cell Death Dis. 1.168055556. PubMed
  45. Guo R, et al. 2020. Nat Microbiol. 0.938194444. PubMed
  46. van der Heiden M, et al. 2020. Scand J Immunol. e13005:. PubMed
  47. Baram T, et al. 2021. Cells. 10:. PubMed
  48. Yang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  49. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  50. Almeida AS, et al. 2021. Bio Protoc. 11:e4012. PubMed
  51. Fronik P, et al. 2021. J Med Chem. 64:12132. PubMed
  52. Webster MR, et al. 2020. Mol Cell. 77:633. PubMed
  53. Lin H, et al. 2020. Front Microbiol. 1.330555556. PubMed
  54. Woodruff MC, et al. 2020. Nat Immunol. 1.920833333. PubMed
  55. Helm M, et al. 2022. Life (Basel). 12:. PubMed
  56. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  57. Cortez JT, et al. 2020. Nature. 582:416. PubMed
  58. Noman MZ, et al. 2020. Sci Adv. 6:eaax7881. PubMed
  59. Prodinger J, et al. 2016. J Leukoc Biol. 99: 583-594. PubMed
  60. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
  61. Ng W, et al. 2021. Pharm Biol. 59:47:00. PubMed
  62. Tian X, et al. 2021. Annals of Translational Medicine. 9(9):781. PubMed
  63. Wei SC, et al. 2020. Cancer Discov. . PubMed
  64. Qi X, et al. 2019. Nat Med. 25:1225. PubMed
  65. Almeida AS, et al. 2021. Bio Protoc. 11:e4012. PubMed
  66. Guo W, et al. 2022. J Immunother Cancer. 10:. PubMed
  67. Shevyrev D, et al. 2021. Exp Ther Med. 209:21. PubMed
  68. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
  69. Freitas JT, et al. 2021. Pigment Cell Melanoma Res. 34:1084. PubMed
  70. Adamik J, et al. 2022. Nat Commun. 13:5184. PubMed
  71. He X, et al. 2021. Adv Sci (Weinh). 8:e2103023. PubMed
  72. Shiao SL, et al. 2021. Cancer Cell. 39:1202. PubMed
  73. Jarjour NN, et al. 2019. Nat Immunol. 1.310416667. PubMed
  74. de Andrade LF, et al. 2019. JCI Insight. 4:e133103. PubMed
  75. Pucella JN, et al. 2019. J Immunol. 202:1383. PubMed
  76. Synn CB, et al. 2022. Clin Transl Immunology. 11:e1364. PubMed
  77. Bunting MD, et al. 2022. Sci Adv. 8:eabk3327. PubMed
  78. Swartz AM, et al. 2022. Curr Protoc. 2:e410. PubMed
  79. Woodruff M, et al. 2020. medRxiv. . PubMed
  80. Goldfarb Y, et al. 2021. J Exp Med. 218:. PubMed
  81. Ellis GI, et al. 2022. STAR Protoc. 3:101784. PubMed
  82. Wiarda JE, et al. 2022. Life Sci Alliance. 5:. PubMed
  83. Hu HJ, et al. 2021. Cancer Immunol Immunother. 70:3541. PubMed
  84. Ye Z, et al. 2021. Nat Commun. 12:907. PubMed
  85. Qiao J, et al. 2019. Cancer Cell. 35:901. PubMed
  86. Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
  87. Lü X, et al. 2021. Exp Ther Med. 22:1005. PubMed
  88. Li Y, et al. 2021. Cell Death Dis. 12:1001. PubMed
  89. Lundtoft C, et al. 2017. PLoS Pathogens. 13(6):e1006425. PubMed
  90. Kleiman E, et al. 2021. Sci Rep. 11:9561. PubMed
  91. Rasmussen TA, et al. 2022. Cell Rep Med. 3:100766. PubMed
  92. Saito E, et al. 2019. Biomaterials. 222:119432. PubMed
  93. Xu Q, et al. 2021. Theranostics. 11:1937. PubMed
  94. Mudd PA, et al. 2022. Cell. 185:603. PubMed
  95. Zhang R, et al. 2022. Front Pharmacol. 13:870848. PubMed
  96. Bin L, et al. 2017. APMIS. 10.1111/apm.12748. PubMed
  97. Ye Z, et al. 2021. NPJ Aging Mech Dis. 7:4. PubMed
  98. Xu L, et al. 2020. Nat Cell Biol. 1162:22. PubMed
  99. Wang G, et al. 2020. Nat Commun. 0.611111111. PubMed
  100. Mirlekar B, et al. 2022. Cell Rep Med. :100744. PubMed
  101. de Picciotto S, et al. 2022. Nat Commun. 13:3866. PubMed
  102. Fang F, et al. 2021. Cell Rep. 37:109981. PubMed
  103. Datlinger P, et al. 2017. Nat Methods. 10.1038/nmeth.4177. PubMed
  104. Jarjour NN, et al. 2020. J Immunol. 204:923. PubMed
  105. Oherle K, et al. 2020. Immunity. 275:52. PubMed
  106. Minns D, et al. 2021. Nat Commun. 12:1285. PubMed
  107. Dhar P, et al. 2021. Commun Biol. 4:905. PubMed
  108. Yao C, et al. 2018. Autophagy. 1.854861111. PubMed
  109. Zhao X, et al. 2022. iScience. 25:104690. PubMed
  110. Okuma A, et al. 2017. Nat Commun.. 10.1038/s41467-017-02281-x. PubMed
  111. Miyauchi K, et al. 2016. Nat Immunol. 17:1447-1458. PubMed
  112. Chen M, et al. 2019. Cell Stem Cell. 25:501. PubMed
  113. Chen C, et al. 2020. Front Cell Neurosci. 14:8. PubMed
  114. Feng Y, et al. 2019. Stem Cell Res Ther. 0.479166667. PubMed
  115. Chen Y, et al. 2022. Nat Commun. 13:4468. PubMed
  116. Capelle CM, et al. 2021. Immunohorizons. 5:711. PubMed
  117. Lone W, et al. 2021. Clin Cancer Res. 27:6039. PubMed
  118. Chen Y, et al. 2021. Nat Immunol. 22:996. PubMed
  119. Bajaña S, et al. 2022. iScience. 25:103732. PubMed
  120. Alhudaithi SS, et al. 2020. Mol Pharm. 17:4691. PubMed
  121. Zhou H, et al. 2021. Cell Death Discov. 7:332. PubMed
  122. Yang X, et al. 2021. Bioact Mater. 3150:6. PubMed
  123. Wang YJ, et al. 2021. Sci Rep. 11:8054. PubMed
  124. Wang R, et al. 2022. J Immunother Cancer. 10:. PubMed
  125. Zhong C, et al. 2021. J Virol. 95:e0092521. PubMed
  126. Smith KJ, et al. 2022. PLoS Biol. 20:e3001554. PubMed
  127. Andricovich J, et al. 2016. J Clin Invest. 126:905-20. PubMed
  128. Prosser A, et al. 2021. STAR Protoc. 2:100810. PubMed
  129. Liu X, et al. 2021. Adv Sci (Weinh). 8:e2100233. PubMed
  130. Zhu P, et al. 2022. Cell Commun Signal. 20:121. PubMed
  131. Zhao J, et al. 2021. Front Oncol. 10:621092. PubMed
  132. Gonzalez MM, et al. 2021. J Clin Invest. 131:. PubMed
  133. Vora AA, et al. 2021. STAR Protocols. 2(2):100565. PubMed
  134. Moritsch S, et al. 2022. Oncoimmunology. 11:2127271. PubMed
  135. Zeng Y, et al. 2019. FASEB J. 33:6596. PubMed
  136. Wang H, et al. 2022. J Cancer. 13:2126. PubMed
  137. Zhang L, et al. 2021. Mol Ther. 29:744. PubMed
  138. Ogawa T, et al. 2020. J Immunol. 205:907. PubMed
  139. Ajith A, et al. 2021. Front Immunol. 12:687715. PubMed
  140. Douglass SM, et al. 2021. Cancer Res. 81:658. PubMed
  141. Capelle CM, et al. 2022. Immunology. 165:428. PubMed
  142. Ellis GI, et al. 2022. Cell Rep Med. 3:100614. PubMed
  143. Grant NL, et al. 2022. Cell Rep. 39:110826. PubMed
  144. Hu M, et al. 2022. Int J Mol Sci. 23: . PubMed
  145. Jou E, et al. 2022. Sci Immunol. 7:eabn0175. PubMed
  146. Yu H, et al. 2022. Hematol Oncol. 40:894. PubMed
  147. Ogishi M, et al. 2023. J Exp Med. 220: . PubMed
  148. Isildar B, et al. 2022. Inflamm Regen. 42:55. PubMed
  149. Noman MZ, et al. 2022. Front Immunol. 13:980704. PubMed
  150. He Y, et al. 2022. Front Immunol. 13:986202. PubMed
  151. Ishii T, et al. 2022. Cell Death Discov. 8:480. PubMed
  152. Chen X, et al. 2022. J Extracell Vesicles. 11:e12279. PubMed
  153. Weng S, et al. 2022. Front Immunol. 13:1025931. PubMed
  154. Landshammer A, et al. 2023. Elife. 12: . PubMed
  155. Vyas M, et al. 2023. Front Immunol. 13:1098445. PubMed
  156. Wang L, et al. 2023. Oxid Med Cell Longev. 2023:6480848. PubMed
  157. Rustagi Y, et al. 2022. Diabetes. 71:1149. PubMed
  158. Zander R, et al. 2022. Immunity. 55:475. PubMed
  159. de Lemos L, et al. 2022. Development. 149:. PubMed
  160. Shen M, et al. 2022. Adv Sci (Weinh). 9:e2203523. PubMed
  161. Cook ME, et al. 2022. Sci Immunol. 7:eabo0981. PubMed
  162. Whyte CE, et al. 2022. Curr Protoc. 2:e589. PubMed
  163. Lao L, et al. 2023. Cancer Immunol Res. 11:320. PubMed
  164. Li Y, et al. 2023. Adv Mater. 35:e2208923. PubMed
  165. Schwarz A, et al. 2023. Front Immunol. 14:1038689. PubMed
  166. Kozlovski S, et al. 2023. Front Immunol. 13:1041552. PubMed
  167. Yi X, et al. 2023. Signal Transduct Target Ther. 8:107. PubMed
  168. Martins Nascentes Melo L, et al. 2023. J Immunother Cancer. 11:. PubMed
  169. McKenzie C, et al. 2023. Mol Ther Nucleic Acids. 32:603. PubMed
  170. Wu C, et al. 2023. Cancer Metab. 11:7. PubMed
  171. Adu-Berchie K, et al. 2023. Nat Commun. 14:3546. PubMed
  172. Wang L, et al. 2019. Front Pharmacol. 1.342361111. PubMed
  173. Manso BA, et al. 2021. STAR Protoc. 2:100485. PubMed
  174. Wu HJ, et al. 2021. Nat Commun. 12:4897. PubMed
  175. Zeng N, et al. 2022. EMBO Rep. 23:e53302. PubMed
  176. Bujanover N, et al. 2021. Stem Cell Reports. 16:1884. PubMed
  177. Kim D, et al. 2021. Nanomicro Lett. 13:31. PubMed
  178. Zhang M, et al. 2020. Nat Commun. 4.707638889. PubMed
  179. Li X, et al. 2022. Nat Commun. 13:2794. PubMed
  180. Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
  181. Hu HJ, et al. 2020. Cell Death Dis. 1.168055556. PubMed
  182. Guo R, et al. 2020. Nat Microbiol. 0.938194444. PubMed
  183. van der Heiden M, et al. 2020. Scand J Immunol. e13005:. PubMed
  184. Baram T, et al. 2021. Cells. 10:. PubMed
  185. Yang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  186. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  187. Almeida AS, et al. 2021. Bio Protoc. 11:e4012. PubMed
  188. Fronik P, et al. 2021. J Med Chem. 64:12132. PubMed
  189. Webster MR, et al. 2020. Mol Cell. 77:633. PubMed
  190. Lin H, et al. 2020. Front Microbiol. 1.330555556. PubMed
  191. Woodruff MC, et al. 2020. Nat Immunol. 1.920833333. PubMed
  192. Helm M, et al. 2022. Life (Basel). 12:. PubMed
  193. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  194. Cortez JT, et al. 2020. Nature. 582:416. PubMed
  195. Noman MZ, et al. 2020. Sci Adv. 6:eaax7881. PubMed
  196. Prodinger J, et al. 2016. J Leukoc Biol. 99: 583-594. PubMed
  197. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
  198. Ng W, et al. 2021. Pharm Biol. 59:47:00. PubMed
  199. Tian X, et al. 2021. Annals of Translational Medicine. 9(9):781. PubMed
  200. Wei SC, et al. 2020. Cancer Discov. . PubMed
  201. Qi X, et al. 2019. Nat Med. 25:1225. PubMed
  202. Almeida AS, et al. 2021. Bio Protoc. 11:e4012. PubMed
  203. Guo W, et al. 2022. J Immunother Cancer. 10:. PubMed
  204. Shevyrev D, et al. 2021. Exp Ther Med. 209:21. PubMed
  205. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
  206. Freitas JT, et al. 2021. Pigment Cell Melanoma Res. 34:1084. PubMed
  207. Adamik J, et al. 2022. Nat Commun. 13:5184. PubMed
  208. He X, et al. 2021. Adv Sci (Weinh). 8:e2103023. PubMed
  209. Shiao SL, et al. 2021. Cancer Cell. 39:1202. PubMed
  210. Jarjour NN, et al. 2019. Nat Immunol. 1.310416667. PubMed
  211. de Andrade LF, et al. 2019. JCI Insight. 4:e133103. PubMed
  212. Pucella JN, et al. 2019. J Immunol. 202:1383. PubMed
  213. Synn CB, et al. 2022. Clin Transl Immunology. 11:e1364. PubMed
  214. Bunting MD, et al. 2022. Sci Adv. 8:eabk3327. PubMed
  215. Swartz AM, et al. 2022. Curr Protoc. 2:e410. PubMed
  216. Woodruff M, et al. 2020. medRxiv. . PubMed
  217. Goldfarb Y, et al. 2021. J Exp Med. 218:. PubMed
  218. Ellis GI, et al. 2022. STAR Protoc. 3:101784. PubMed
  219. Wiarda JE, et al. 2022. Life Sci Alliance. 5:. PubMed
  220. Hu HJ, et al. 2021. Cancer Immunol Immunother. 70:3541. PubMed
  221. Ye Z, et al. 2021. Nat Commun. 12:907. PubMed
  222. Qiao J, et al. 2019. Cancer Cell. 35:901. PubMed
  223. Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
  224. Lü X, et al. 2021. Exp Ther Med. 22:1005. PubMed
  225. Li Y, et al. 2021. Cell Death Dis. 12:1001. PubMed
  226. Lundtoft C, et al. 2017. PLoS Pathogens. 13(6):e1006425. PubMed
  227. Kleiman E, et al. 2021. Sci Rep. 11:9561. PubMed
  228. Rasmussen TA, et al. 2022. Cell Rep Med. 3:100766. PubMed
  229. Saito E, et al. 2019. Biomaterials. 222:119432. PubMed
  230. Xu Q, et al. 2021. Theranostics. 11:1937. PubMed
  231. Mudd PA, et al. 2022. Cell. 185:603. PubMed
  232. Zhang R, et al. 2022. Front Pharmacol. 13:870848. PubMed
  233. Bin L, et al. 2017. APMIS. 10.1111/apm.12748. PubMed
  234. Ye Z, et al. 2021. NPJ Aging Mech Dis. 7:4. PubMed
  235. Xu L, et al. 2020. Nat Cell Biol. 1162:22. PubMed
  236. Wang G, et al. 2020. Nat Commun. 0.611111111. PubMed
  237. Mirlekar B, et al. 2022. Cell Rep Med. :100744. PubMed
  238. de Picciotto S, et al. 2022. Nat Commun. 13:3866. PubMed
  239. Fang F, et al. 2021. Cell Rep. 37:109981. PubMed
  240. Datlinger P, et al. 2017. Nat Methods. 10.1038/nmeth.4177. PubMed
  241. Jarjour NN, et al. 2020. J Immunol. 204:923. PubMed
  242. Oherle K, et al. 2020. Immunity. 275:52. PubMed
  243. Minns D, et al. 2021. Nat Commun. 12:1285. PubMed
  244. Dhar P, et al. 2021. Commun Biol. 4:905. PubMed
  245. Yao C, et al. 2018. Autophagy. 1.854861111. PubMed
  246. Zhao X, et al. 2022. iScience. 25:104690. PubMed
  247. Okuma A, et al. 2017. Nat Commun.. 10.1038/s41467-017-02281-x. PubMed
  248. Miyauchi K, et al. 2016. Nat Immunol. 17:1447-1458. PubMed
  249. Chen M, et al. 2019. Cell Stem Cell. 25:501. PubMed
  250. Chen C, et al. 2020. Front Cell Neurosci. 14:8. PubMed
  251. Feng Y, et al. 2019. Stem Cell Res Ther. 0.479166667. PubMed
  252. Chen Y, et al. 2022. Nat Commun. 13:4468. PubMed
  253. Capelle CM, et al. 2021. Immunohorizons. 5:711. PubMed
  254. Lone W, et al. 2021. Clin Cancer Res. 27:6039. PubMed
  255. Chen Y, et al. 2021. Nat Immunol. 22:996. PubMed
  256. Bajaña S, et al. 2022. iScience. 25:103732. PubMed
  257. Alhudaithi SS, et al. 2020. Mol Pharm. 17:4691. PubMed
  258. Zhou H, et al. 2021. Cell Death Discov. 7:332. PubMed
  259. Yang X, et al. 2021. Bioact Mater. 3150:6. PubMed
  260. Wang YJ, et al. 2021. Sci Rep. 11:8054. PubMed
  261. Wang R, et al. 2022. J Immunother Cancer. 10:. PubMed
  262. Zhong C, et al. 2021. J Virol. 95:e0092521. PubMed
  263. Smith KJ, et al. 2022. PLoS Biol. 20:e3001554. PubMed
  264. Andricovich J, et al. 2016. J Clin Invest. 126:905-20. PubMed
  265. Prosser A, et al. 2021. STAR Protoc. 2:100810. PubMed
  266. Liu X, et al. 2021. Adv Sci (Weinh). 8:e2100233. PubMed
  267. Zhu P, et al. 2022. Cell Commun Signal. 20:121. PubMed
  268. Zhao J, et al. 2021. Front Oncol. 10:621092. PubMed
  269. Gonzalez MM, et al. 2021. J Clin Invest. 131:. PubMed
  270. Vora AA, et al. 2021. STAR Protocols. 2(2):100565. PubMed
  271. Moritsch S, et al. 2022. Oncoimmunology. 11:2127271. PubMed
  272. Zeng Y, et al. 2019. FASEB J. 33:6596. PubMed
  273. Wang H, et al. 2022. J Cancer. 13:2126. PubMed
  274. Zhang L, et al. 2021. Mol Ther. 29:744. PubMed

Antigen Details

Cell Type
Tregs
Biology Area
Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Gene ID
NA

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Go To Top Version: 5    Revision Date: 05/20/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

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Pricing & Availability
Regulatory Status
RUO
Other Names
Transcription Factor Fixation/Permeabilization Buffer Set
Ave. Rating
Submit a Review
Product Citations
publications
Transcription_Factor_F_P_Buffer_1_ICFC_012915
Human peripheral blood lymphocytes were surface stained with CD3 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with T-bet (clone 4B10) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • Transcription_Factor_F_P_Buffer_1_ICFC_012915
    Human peripheral blood lymphocytes were surface stained with CD3 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with T-bet (clone 4B10) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • Transcription_Factor_F_P_Buffer_2_ICFC_012915
Cat # Size Price Quantity Check Availability Save
424401 120 tests DKK981
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Description

BioLegend's True-Nuclear™ Transcription Factor Buffer Set has been specially formulated for intracellular staining with minimum effect on the surface fluorochrome staining.

See the advantages of True-Nuclear™ Transcription Factor Buffer Set in this brief webinar: biolegend.com/true_nuclear_webinar.


 

Caution: The True-Nuclear™ Transcription Factor Buffer Set contains paraformaldehyde, which is toxic and mutagenic. Please handle with caution. Wear gloves, lab coats, and necessary protection to avoid direct contact. 

Product Details
Technical Data Sheet (pdf)

Product Details

Storage & Handling
Store between 2°C and 8°C. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Prepare fresh Transcription Factor Fix working solution by diluting the 4X Fix Concentrate (1 part) with the Fix Diluent (3 parts). 1 mL of the 1X Fix working solution is needed for each tube.

Prepare a 1X working solution of the Perm Buffer by diluting the 10X Perm Buffer with distilled water. 6.5 mL of 1X Perm Buffer is needed for each sample of tube.

NOTE: The 10X Perm Buffer may have crystallization or precipitation when it is stored at 2-8°C; however, this is normal and does not affect the buffer's performance. If there is a heavy precipitation observed after dilution to 1X working solution, it can be filtered to clarify the solution.

Application Notes

Buffer Set Contents:

  • True-Nuclear™ 4X Fix Concentrate (30 mL)
  • True-Nuclear™ Fix Diluent (100 mL)
  • True-Nuclear™ 10X Perm Buffer (100 mL)

 

Additional Product Notes

View more applications data using this product to stain Veri-Cells™ lyophilized control cells, to detect transcription factors, and to study TIGIT in our Scientific Poster Library.

Product Citations
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  151. Ishii T, et al. 2022. Cell Death Discov. 8:480. PubMed
  152. Chen X, et al. 2022. J Extracell Vesicles. 11:e12279. PubMed
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  172. Wang L, et al. 2019. Front Pharmacol. 1.342361111. PubMed
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  186. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  187. Almeida AS, et al. 2021. Bio Protoc. 11:e4012. PubMed
  188. Fronik P, et al. 2021. J Med Chem. 64:12132. PubMed
  189. Webster MR, et al. 2020. Mol Cell. 77:633. PubMed
  190. Lin H, et al. 2020. Front Microbiol. 1.330555556. PubMed
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  192. Helm M, et al. 2022. Life (Basel). 12:. PubMed
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  194. Cortez JT, et al. 2020. Nature. 582:416. PubMed
  195. Noman MZ, et al. 2020. Sci Adv. 6:eaax7881. PubMed
  196. Prodinger J, et al. 2016. J Leukoc Biol. 99: 583-594. PubMed
  197. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
  198. Ng W, et al. 2021. Pharm Biol. 59:47:00. PubMed
  199. Tian X, et al. 2021. Annals of Translational Medicine. 9(9):781. PubMed
  200. Wei SC, et al. 2020. Cancer Discov. . PubMed
  201. Qi X, et al. 2019. Nat Med. 25:1225. PubMed
  202. Almeida AS, et al. 2021. Bio Protoc. 11:e4012. PubMed
  203. Guo W, et al. 2022. J Immunother Cancer. 10:. PubMed
  204. Shevyrev D, et al. 2021. Exp Ther Med. 209:21. PubMed
  205. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
  206. Freitas JT, et al. 2021. Pigment Cell Melanoma Res. 34:1084. PubMed
  207. Adamik J, et al. 2022. Nat Commun. 13:5184. PubMed
  208. He X, et al. 2021. Adv Sci (Weinh). 8:e2103023. PubMed
  209. Shiao SL, et al. 2021. Cancer Cell. 39:1202. PubMed
  210. Jarjour NN, et al. 2019. Nat Immunol. 1.310416667. PubMed
  211. de Andrade LF, et al. 2019. JCI Insight. 4:e133103. PubMed
  212. Pucella JN, et al. 2019. J Immunol. 202:1383. PubMed
  213. Synn CB, et al. 2022. Clin Transl Immunology. 11:e1364. PubMed
  214. Bunting MD, et al. 2022. Sci Adv. 8:eabk3327. PubMed
  215. Swartz AM, et al. 2022. Curr Protoc. 2:e410. PubMed
  216. Woodruff M, et al. 2020. medRxiv. . PubMed
  217. Goldfarb Y, et al. 2021. J Exp Med. 218:. PubMed
  218. Ellis GI, et al. 2022. STAR Protoc. 3:101784. PubMed
  219. Wiarda JE, et al. 2022. Life Sci Alliance. 5:. PubMed
  220. Hu HJ, et al. 2021. Cancer Immunol Immunother. 70:3541. PubMed
  221. Ye Z, et al. 2021. Nat Commun. 12:907. PubMed
  222. Qiao J, et al. 2019. Cancer Cell. 35:901. PubMed
  223. Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
  224. Lü X, et al. 2021. Exp Ther Med. 22:1005. PubMed
  225. Li Y, et al. 2021. Cell Death Dis. 12:1001. PubMed
  226. Lundtoft C, et al. 2017. PLoS Pathogens. 13(6):e1006425. PubMed
  227. Kleiman E, et al. 2021. Sci Rep. 11:9561. PubMed
  228. Rasmussen TA, et al. 2022. Cell Rep Med. 3:100766. PubMed
  229. Saito E, et al. 2019. Biomaterials. 222:119432. PubMed
  230. Xu Q, et al. 2021. Theranostics. 11:1937. PubMed
  231. Mudd PA, et al. 2022. Cell. 185:603. PubMed
  232. Zhang R, et al. 2022. Front Pharmacol. 13:870848. PubMed
  233. Bin L, et al. 2017. APMIS. 10.1111/apm.12748. PubMed
  234. Ye Z, et al. 2021. NPJ Aging Mech Dis. 7:4. PubMed
  235. Xu L, et al. 2020. Nat Cell Biol. 1162:22. PubMed
  236. Wang G, et al. 2020. Nat Commun. 0.611111111. PubMed
  237. Mirlekar B, et al. 2022. Cell Rep Med. :100744. PubMed
  238. de Picciotto S, et al. 2022. Nat Commun. 13:3866. PubMed
  239. Fang F, et al. 2021. Cell Rep. 37:109981. PubMed
  240. Datlinger P, et al. 2017. Nat Methods. 10.1038/nmeth.4177. PubMed
  241. Jarjour NN, et al. 2020. J Immunol. 204:923. PubMed
  242. Oherle K, et al. 2020. Immunity. 275:52. PubMed
  243. Minns D, et al. 2021. Nat Commun. 12:1285. PubMed
  244. Dhar P, et al. 2021. Commun Biol. 4:905. PubMed
  245. Yao C, et al. 2018. Autophagy. 1.854861111. PubMed
  246. Zhao X, et al. 2022. iScience. 25:104690. PubMed
  247. Okuma A, et al. 2017. Nat Commun.. 10.1038/s41467-017-02281-x. PubMed
  248. Miyauchi K, et al. 2016. Nat Immunol. 17:1447-1458. PubMed
  249. Chen M, et al. 2019. Cell Stem Cell. 25:501. PubMed
  250. Chen C, et al. 2020. Front Cell Neurosci. 14:8. PubMed
  251. Feng Y, et al. 2019. Stem Cell Res Ther. 0.479166667. PubMed
  252. Chen Y, et al. 2022. Nat Commun. 13:4468. PubMed
  253. Capelle CM, et al. 2021. Immunohorizons. 5:711. PubMed
  254. Lone W, et al. 2021. Clin Cancer Res. 27:6039. PubMed
  255. Chen Y, et al. 2021. Nat Immunol. 22:996. PubMed
  256. Bajaña S, et al. 2022. iScience. 25:103732. PubMed
  257. Alhudaithi SS, et al. 2020. Mol Pharm. 17:4691. PubMed
  258. Zhou H, et al. 2021. Cell Death Discov. 7:332. PubMed
  259. Yang X, et al. 2021. Bioact Mater. 3150:6. PubMed
  260. Wang YJ, et al. 2021. Sci Rep. 11:8054. PubMed
  261. Wang R, et al. 2022. J Immunother Cancer. 10:. PubMed
  262. Zhong C, et al. 2021. J Virol. 95:e0092521. PubMed
  263. Smith KJ, et al. 2022. PLoS Biol. 20:e3001554. PubMed
  264. Andricovich J, et al. 2016. J Clin Invest. 126:905-20. PubMed
  265. Prosser A, et al. 2021. STAR Protoc. 2:100810. PubMed
  266. Liu X, et al. 2021. Adv Sci (Weinh). 8:e2100233. PubMed
  267. Zhu P, et al. 2022. Cell Commun Signal. 20:121. PubMed
  268. Zhao J, et al. 2021. Front Oncol. 10:621092. PubMed
  269. Gonzalez MM, et al. 2021. J Clin Invest. 131:. PubMed
  270. Vora AA, et al. 2021. STAR Protocols. 2(2):100565. PubMed
  271. Moritsch S, et al. 2022. Oncoimmunology. 11:2127271. PubMed
  272. Zeng Y, et al. 2019. FASEB J. 33:6596. PubMed
  273. Wang H, et al. 2022. J Cancer. 13:2126. PubMed
  274. Zhang L, et al. 2021. Mol Ther. 29:744. PubMed

Antigen Details

Cell Type
Tregs
Biology Area
Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Gene ID
NA
Go To Top Version: 5    Revision Date: 05/20/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

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