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True-Stain Monocyte Blocker™ True-Stain Monocyte Blocker™

Pricing & Availability
Regulatory Status
RUO
Other Names
Cyanine dye blocking solution
Ave. Rating
Submit a Review
Product Citations
publications
True-Stain_Monocyte_Blocker_3-plot_030519
Human peripheral blood mononuclear cells were either untreated (black) or treated with True-Stain Monocyte Blocker™ (red) and stained with CD14 (clone HCD14) Brilliant Violet™ 421 and CD19 (clone HIB19) PE/Dazzle™ 594 (left), CD3 (clone UCHT1) APC/Cyanine7 (middle), or CD56 (clone 5.1H11) PE/Cyanine5.
  • True-Stain_Monocyte_Blocker_3-plot_030519
    Human peripheral blood mononuclear cells were either untreated (black) or treated with True-Stain Monocyte Blocker™ (red) and stained with CD14 (clone HCD14) Brilliant Violet™ 421 and CD19 (clone HIB19) PE/Dazzle™ 594 (left), CD3 (clone UCHT1) APC/Cyanine7 (middle), or CD56 (clone 5.1H11) PE/Cyanine5.
Cat # Size Price Quantity Check Availability Save
426101 25 tests DKK280
Check Availability


Need larger quantities of this item?
Request Bulk Quote
426102 100 tests DKK838
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Need larger quantities of this item?
Request Bulk Quote
426103 500 tests DKK3345
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Need larger quantities of this item?
Request Bulk Quote
Description

True-Stain Monocyte Blocker™ has been formulated to block non-specific binding of PE/Dazzle™ 594, PE/Cyanine5, PE/Cyanine7, APC/Cyanine7, APC/Fire™ 750, and PE/Fire™ 744 commonly seen on monocytes and macrophages. The blocker is most effective when doing surface staining on live cells prior to the fixation step in a staining protocol.    Learn more.

Product Details
Technical Data Sheet (pdf)

Product Details

Storage & Handling
Upon receipt, store between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Application Notes

Add 5 µl of the True-Stain Monocyte Blocker™ to a suspension of cells immediately prior to the addition of antibodies, or mixed into the antibody cocktail, so that the final staining volume will equal 100 µl. This step can be performed at room temperature or on ice (2-8°C).

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

Product Citations
  1. Coronel MM, et al. 2020. Sci Adv. 6:eaba5573. PubMed
  2. Herland A, et al. 2020. Nat Biomed Eng. 421:4. PubMed
  3. Santopaolo M, et al. 2021. Front Immunol. 609406:12. PubMed
  4. Coronel MM, et al. 2020. Sci Adv. 6:eaba5573. PubMed
  5. Herland A, et al. 2020. Nat Biomed Eng. 421:4. PubMed
  6. Santopaolo M, et al. 2021. Front Immunol. 609406:12. PubMed

Antigen Details

Gene ID
NA
Go To Top Version: 1    Revision Date: 05/30/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

Pricing & Availability
Regulatory Status
RUO
Other Names
Cyanine dye blocking solution
Ave. Rating
Submit a Review
Product Citations
publications
True-Stain_Monocyte_Blocker_3-plot_030519
Human peripheral blood mononuclear cells were either untreated (black) or treated with True-Stain Monocyte Blocker™ (red) and stained with CD14 (clone HCD14) Brilliant Violet™ 421 and CD19 (clone HIB19) PE/Dazzle™ 594 (left), CD3 (clone UCHT1) APC/Cyanine7 (middle), or CD56 (clone 5.1H11) PE/Cyanine5.
  • True-Stain_Monocyte_Blocker_3-plot_030519
    Human peripheral blood mononuclear cells were either untreated (black) or treated with True-Stain Monocyte Blocker™ (red) and stained with CD14 (clone HCD14) Brilliant Violet™ 421 and CD19 (clone HIB19) PE/Dazzle™ 594 (left), CD3 (clone UCHT1) APC/Cyanine7 (middle), or CD56 (clone 5.1H11) PE/Cyanine5.
Cat # Size Price Quantity Check Availability Save
426101 25 tests DKK280
Check Availability


Need larger quantities of this item?
Request Bulk Quote
426102 100 tests DKK838
Check Availability


Need larger quantities of this item?
Request Bulk Quote
426103 500 tests DKK3345
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

True-Stain Monocyte Blocker™ has been formulated to block non-specific binding of PE/Dazzle™ 594, PE/Cyanine5, PE/Cyanine7, APC/Cyanine7, APC/Fire™ 750, and PE/Fire™ 744 commonly seen on monocytes and macrophages. The blocker is most effective when doing surface staining on live cells prior to the fixation step in a staining protocol.    Learn more.

Product Details
Technical Data Sheet (pdf)

Product Details

Storage & Handling
Upon receipt, store between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Application Notes

Add 5 µl of the True-Stain Monocyte Blocker™ to a suspension of cells immediately prior to the addition of antibodies, or mixed into the antibody cocktail, so that the final staining volume will equal 100 µl. This step can be performed at room temperature or on ice (2-8°C).

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

Product Citations
  1. Coronel MM, et al. 2020. Sci Adv. 6:eaba5573. PubMed
  2. Herland A, et al. 2020. Nat Biomed Eng. 421:4. PubMed
  3. Santopaolo M, et al. 2021. Front Immunol. 609406:12. PubMed
  4. Coronel MM, et al. 2020. Sci Adv. 6:eaba5573. PubMed
  5. Herland A, et al. 2020. Nat Biomed Eng. 421:4. PubMed
  6. Santopaolo M, et al. 2021. Front Immunol. 609406:12. PubMed

Antigen Details

Gene ID
NA
Go To Top Version: 1    Revision Date: 05/30/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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