Loading...

Fixation Buffer

Pricing & Availability
Regulatory Status
RUO
Other Names
Fixative, Paraformaldehyde
Ave. Rating
Submit a Review
Product Citations
publications
Cat # Size Price Quantity Check Availability Save
420801 100 mL 48€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Fixation Buffer is useful for intracellular staining procedures, e.g., in preparation of cells for staining intracellular cytokines or other proteins. Fixation Buffer is used to fix cells prior to permeabilization using Permeabilization Wash Buffer (Cat. No. 421002). BioLegend's Fixation Buffer has been formulated with prescreened paraformaldehyde with low background, thus producing the greatest signal to noise ratio.

Product Details
Technical Data Sheet (pdf)

Product Details

Storage & Handling
This buffer solution should be stored between 2°C and 8°C.
Application

ICFC - Quality tested
ICC
Recommended Usage

For cell fixation, use 250 µL fixation buffer per tube and leave it in the dark for 20 minutes at room temperature. It is recommended that the reagent be titrated for optimal performance for each application. For the fixation procedure, please refer to the "Intracellular Cytokine Staining Protocol" under "Support" on BioLegend's website.
Caution: This buffer contains paraformaldehyde, which is toxigenic and mutagenic. Please handle with caution and wear gloves, lab coat and necessary protection to avoid direct body contacts.

Application Notes

This 1X PBS solution contains 4% paraformaldehyde, which is toxic and is a suspected carcinogen. Contact with eyes, skin and mucous membranes should be avoided.

Additional Product Notes

View more applications data using this product to stain Veri-Cells™ lyophilized control cells and to perform phospho-flow in our Scientific Poster Library.

Application References
  1. Kang YJ, et al. 2007. Nature Immunol. 8:601.
  2. Kenna TJ, et al. 2010. J. Immunol. 184:598. PubMed
  3. Sullivan BP, et al. 2010. Am J Pathol. 177:2837. PubMed
  4. del Rio ML, et al. 2011.Transplantation. 92:1085. PubMed
  5. del Rio ML, et al. 2012. J. Immunol. 188:4885. PubMed
  6. Marongiu L, et al. 2013. PLoS One. 8:75684. PubMed
  7. Haberthur K, et al. 2013. J Virol. 87:11751. PubMed
  8. Busskamp V, et al. 2014. Mol Syst Biol. 10:760. PubMed
Product Citations
  1. Darzianiazizi M, et al. 2020. Int J Mol Sci. 21:00. PubMed
  2. Mirlekar B, et al. 2020. Cancer Immunol Res. 0.536111111. PubMed
  3. Yokozeki Y, et al. 2021. Biomed Res Int. 2021:7988320. PubMed
  4. Tocheva AS, et al. 2020. Curr Protoc Immunol. 130:e103. PubMed
  5. Salybekov AA, et al. 2018. PLoS One. 13:e0203244. PubMed
  6. Shen Z, et al. 2020. Bioact Mater. 5:1113. PubMed
  7. Kim J, et al. 2021. Cytometry A. 99:807. PubMed
  8. Stoycheva D, et al. 2021. Eur J Immunol. 51:1423. PubMed
  9. Bruno MEC, et al. 2022. J Cell Physiol. 237:706. PubMed
  10. Mills EL, et al. 2022. Cell Metab. 34:140. PubMed
  11. Bettke JA, et al. 2022. Infect Immun. 90:e0007022. PubMed
  12. Madi A, et al. 2022. Int J Cancer. 151:797. PubMed
  13. Yue Y, et al. 2022. Nat Biomed Eng. 6:898. PubMed
  14. Sulaj A, et al. 2022. J Clin Endocrinol Metab. 107:2167. PubMed
  15. Brog RA, et al. 2022. Cancer Immunol Res. 10:962. PubMed
  16. Chen ML, et al. 2022. Mol Cancer Ther. 21:1337. PubMed
  17. Yin XY, et al. 2023. Neural Regen Res. 18:213. PubMed
  18. Gutierrez WR, et al. 2022. JCI Insight. 7: . PubMed
  19. Wang Y, et al. 2022. Front Endocrinol (Lausanne). 13:1036517. PubMed
  20. Koutník J, et al. 2022. Front Immunol. 13:1049033. PubMed
  21. Minott JA, et al. 2022. Front Immunol. 13:1038340. PubMed
  22. Tan TCJ, et al. 2022. Nat Commun. 13:7796. PubMed
  23. Noh J, et al. 2022. Cancers (Basel). 14: . PubMed
  24. Phares TW, et al. 2022. Front Pharmacol. 13:1029636. PubMed
  25. Zhao X, et al. 2022. STAR Protoc. 3:101859. PubMed
  26. Chan L, et al. 2022. Cells. 12: . PubMed
  27. Qin S, et al. 2023. Acta Pharm Sin B. :. PubMed
  28. Gong HH, et al. 2023. Front Immunol. 13:1089064. PubMed
  29. Teplensky MH, et al. 2023. Nat Biomed Eng. :. PubMed
  30. Sun L, et al. 2023. Mol Med Rep. 27: . PubMed
  31. Chan L, et al. 2023. Int J Mol Sci. 24: . PubMed
  32. Wang L, et al. 2023. Oxid Med Cell Longev. 2023:6480848. PubMed
  33. Xiao JF, et al. 2022. Mol Cancer Res. 20:637. PubMed
  34. Zander R, et al. 2022. Immunity. 55:475. PubMed
  35. Zhang H, et al. 2022. J Exp Med. 219:. PubMed
  36. Bronge M, et al. 2022. Sci Adv. 8:eabn1823. PubMed
  37. Zhuo Y, et al. 2022. J Immunother Cancer. 10:. PubMed
  38. Tipanee J, et al. 2022. Mol Ther. 30:3155. PubMed
  39. Tang J, et al. 2022. Sci Immunol. 7:eadd4853. PubMed
  40. de Groot AE, et al. 2022. Neoplasia. 32:100830. PubMed
  41. Hajnik RL, et al. 2022. Sci Transl Med. 14:eabq1945. PubMed
  42. Frost JN, et al. 2022. Sci Adv. 8:eabq5384. PubMed
  43. Li L, et al. 2022. Cancer Immunol Res. 10:1475. PubMed
  44. Chernozem RV, et al. 2023. Adv Healthc Mater. 12:e2201726. PubMed
  45. Gao L, et al. 2023. J Nanobiotechnology. 21:56. PubMed
  46. Kodama N, et al. 2023. Front Nutr. 10:1081263. PubMed
  47. Humphries DC, et al. 2023. Front Immunol. 14:1100161. PubMed
  48. Wang H, et al. 2023. Nature. 615:339. PubMed
  49. Gayet RV, et al. 2023. Nat Commun. 14:1339. PubMed
  50. Varun K, et al. 2023. EBioMedicine. 90:104516. PubMed
  51. Wang YH, et al. 2023. Int J Mol Sci. 24:. PubMed
  52. Deng G, et al. 2023. Cancers (Basel). 15:. PubMed
  53. Wang Y, et al. 2023. iScience. 26:106630. PubMed
  54. Xie J, et al. 2023. Mol Ther Oncolytics. 29:61. PubMed
  55. Amirova KM, et al. 2023. Int J Mol Sci. 24:. PubMed
  56. Ruan H, et al. 2023. Acta Pharm Sin B. 13:2202. PubMed
  57. Zhang TR, et al. 2023. Pharmaceuticals (Basel). 16:. PubMed
  58. Shou J, et al. 2023. Sci Adv. 9:eade9118. PubMed
  59. Michalaki C, et al. 2022. Curr Protoc. 2:e505. PubMed
  60. Haberthur K, et al. 2013. J Virol. 87:11751. PubMed
  61. Li B, Schmidt N 2016. PLoS One. 11: 0162427. PubMed
  62. Lentucci C, et al. 2017. J Biol Chem. 292:2754-2772. PubMed
  63. Spitzer SO, et al. 2019. Neuron. 101:459. PubMed
  64. Li Q, et al. 2022. Cell Rep. 40:111308. PubMed
  65. Albaghdadi AJH, et al. 2022. Int J Mol Sci. 23:. PubMed
  66. van Vloten JP, et al. 2022. J Immunother Cancer. 10:. PubMed
  67. Zhang L, et al. 2021. J Immunol. 207:590. PubMed
  68. Li X, et al. 2022. Nat Commun. 13:2794. PubMed
  69. Li L,et al. 2017. Sci Rep.. 10.1038/s41598-017-14000-z. PubMed
  70. Ede J, et al. 2015. Toxicol Sci. 148: 108 - 120. PubMed
  71. Jimenez-Duran G, et al. 2020. EBioMedicine. 61:103039. PubMed
  72. Zhang Y, et al. 2020. Oncol Lett. 1.053472222. PubMed
  73. Lownik JC, et al. 2020. Biochem Biophys Res Commun. 522:442. PubMed
  74. Angiari S, et al. 2020. Cell Metab. 31:391. PubMed
  75. Garton J, et al. 2021. Immunohorizons. 5:802. PubMed
  76. Duan Q, et al. 2021. Front Cell Dev Biol. 9:761193. PubMed
  77. Stegelmeier AA, et al. 2022. Biomedicines. 10:. PubMed
  78. Thaker YR, et al. 2022. Front Oncol. 12:884196. PubMed
  79. de Groot AE, et al. 2021. Prostate. 81:629. PubMed
  80. Kuo T, et al. 2017. Oncogene. 10.1038/onc.2017.156. PubMed
  81. Zhang S, et al. 2022. Cell Death Dis. 13:844. PubMed
  82. Busskamp V, et al. 2014. Mol Syst Biol. 10:760. PubMed
  83. Kakiyama G, et al. 2020. J Lipid Res. 61:1629. PubMed
  84. Brooks JF, et al. 2020. J Immunol. 205:1239. PubMed
  85. Jenull S, et al. 2021. Cell Reports. 36(3):109406. PubMed
  86. Kakiyama G, et al. 2020. J Lipid Res. 61:1629. PubMed
  87. Martinez-Usatorre A, et al. 2020. Front Immunol. 11:340. PubMed
  88. Martinez–Usatorre A, et al. 2019. Cancer Immunol Res. 0.995138889. PubMed
  89. Tsai CY, et al. 2022. J Biomed Sci. 29:47. PubMed
  90. van der Heide V, et al. 2022. Cell Rep. :110508. PubMed
  91. Bawazir M, et al. 2022. Front Immunol. 13:1033794. PubMed
  92. Wright A, et al. 2015. Immunobiology. 220: 859-864. PubMed
  93. Tang B, et al. 2020. Clin Cancer Res. 26:2216. PubMed
  94. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  95. Herda S, et al. 2021. Int J Cancer. 148:3097. PubMed
  96. Haryadi L, et al. 2019. Vet World. 12:1529. PubMed
  97. Bayer AL, et al. 2022. Cancers (Basel). 14:. PubMed
  98. Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed
  99. Eslani M,et al. 2017. Invest Ophthalmol Vis Sci.. 58(12):5507-5517. PubMed
  100. Guye P, et al. 2016. Nat Commun. 7:10243. PubMed
  101. Shamsi F, et al. 2021. Nat Metab. 3:485. PubMed
  102. Zhang X, et al. 2020. J Immunol. 205:1743. PubMed
  103. Su W, et al. 2022. Front Immunol. 13:952338. PubMed
  104. Datta A, et al. 2022. J Fungi (Basel). 8:. PubMed
  105. Wu Q, et al. 2022. Cell Mol Life Sci. 79:137. PubMed
  106. Li Y, et al. 2022. Clin Transl Immunology. 11:e1362. PubMed
  107. Subham S, et al. 2022. Breast Cancer Res Treat. Online ahead of print. PubMed
  108. Cheng K, et al. 2021. Nat Commun. 12:2041. PubMed
  109. Klepsch V, et al. 2020. Cell Commun Signal. 18:08. PubMed
  110. Sullivan B, et al. 2010. Am J Pathol. 177:2837. PubMed
  111. Zhang B, et al. 2022. J Exp Med. 219:. PubMed
  112. Callahan SM, et al. 2021. Curr Protoc. 1:e294. PubMed
  113. Huang M, et al. 2022. Adv Sci (Weinh). 9:e2101267. PubMed
  114. Li FJ, et al. 2022. PLoS One. 17:e0277019. PubMed
  115. Chen L, et al. 2021. Cell Death Differ. 28:1880. PubMed
  116. Park HB, et al. 2020. Oncoimmunology. 9:1772663. PubMed
  117. Zhang L, et al. 2021. Methods Mol Biol. 2388:175. PubMed
  118. Rada M, et al. 2021. Commun Biol. 4:950. PubMed
  119. Chakraborty S, et al. 2022. Sci Transl Med. . PubMed
  120. Leonard NA, et al. 2021. Cancers (Basel). 13:. PubMed
  121. Takahashi K, et al. 2022. Cell Rep Methods. 2:100155. PubMed
  122. Cho S, et al. 2017. Sci Rep. 7:35. PubMed
  123. Callahan S, et al. 2020. Cell Microbiol. 22:e13210. PubMed
  124. Jimenez RV, et al. 2019. Front Immunol. 1.932638889. PubMed
  125. Elliott PR, et al. 2021. Cell Rep. 37:109777. PubMed
  126. Balan I, et al. 2022. Front Immunol. 13:940095. PubMed
  127. Bunting MD, et al. 2022. Sci Adv. 8:eabk3327. PubMed
  128. Li J, et al. 2022. J Tissue Eng. 13:20417314221127908. PubMed
  129. van Duijn A, et al. 2021. Cancer Immunol Immunother. Online ahead of print. PubMed
  130. Jakic B, et al. 2021. Cell Death Dis. 12:187. PubMed
  131. Yang J, et al. 2022. Microbiome. 10:149. PubMed
  132. Alvandi Z, et al. 2022. J Am Heart Assoc. 11:e023695. PubMed
  133. Wei W, et al. 2022. mSystems. 7:e0046922. PubMed
  134. Zhao X, et al. 2022. Nat Protoc. 17:2240. PubMed
  135. Lamb C, et al. 2016. J Crohns Colitis. 10.1093/ecco-jcc/jjw189. PubMed
  136. Xu Z, et al. 2016. Nat Commun. 7:10728. PubMed
  137. Williamson LM, et al. 2021. NPJ Precis Oncol. 5:103. PubMed
  138. Gunasena M, et al. 2022. Sci Rep. 12:12377. PubMed
  139. Hornigold K, et al. 2022. Front Immunol. 13:888415. PubMed
  140. Yang B, et al. 2022. Bioengineered. 13:2685. PubMed
  141. Li Q, et al. 2017. Front Immunol. 0.797916667. PubMed
  142. Bekeschus S, et al. 2016. J Leukoc Biol. 100: 791 - 799. PubMed
  143. Wang L, et al. 2020. EMBO J. 39:e104514. PubMed
  144. Rio M, et al. 2012. J Immunol. 188:4885. PubMed
  145. Kundu K, et al. 2019. Cancer Immunol Res. 1.069444444. PubMed
  146. Willis RA, et al. 2021. Curr Protoc. 1:e36. PubMed
  147. Chen DL, et al. 2021. Mol Cancer. 20:166. PubMed
  148. Bajpai G, et al. 2018. Nat Med. 24:1234. PubMed
  149. Wilmes A, et al. 2017. Toxicol In Vitro. 10.1016/j.tiv.2017.07.023. PubMed
  150. Lundtoft C, et al. 2017. PLoS Pathogens. 13(6):e1006425. PubMed
  151. Liu X, et al. 2016. Sci Rep. 6:36722. PubMed
  152. Dominguez CX, et al. 2020. Cancer Discov. 0.577777778. PubMed
  153. Wei Z, et al. 2021. Nat Commun. 0.805555556. PubMed
  154. Hiza H, et al. 2021. Sci Rep. 11:13190. PubMed
  155. Chen YF, et al. 2019. J Biomed Sci. 26:85. PubMed
  156. Rio M, et al. 2011. Transplantation. 92:1085. PubMed
  157. Xu Q, et al. 2021. Theranostics. 11:1937. PubMed
  158. Wen T, et al. 2022. Cancer Immunol Res. 10:162. PubMed
  159. Ma K, et al. 2022. iScience. 25:104347. PubMed
  160. Constant DA, et al. 2022. Immunohorizons. 6:416. PubMed
  161. Koyama T, et al. 2022. Curr Issues Mol Biol. 44:3146. PubMed
  162. Kakiyama G, et al. 2020. J Lipid Res. 61:1629. PubMed
  163. Wang Y, et al. 2020. Virol Sin. 36:122. PubMed
  164. Gutierrez WR, et al. 2021. Sci Rep. 1.220833333. PubMed
  165. Kenna T, et al. 2010. J Immunol. 184:598. PubMed
  166. Mirlekar B, et al. 2022. Cell Rep Med. :100744. PubMed
  167. Rive CM, et al. 2022. Mol Ther Methods Clin Dev. 26:4. PubMed
  168. Bruno MEC, et al. 2022. Geroscience. 44:1761. PubMed
  169. Teh MR, et al. 2021. Front Immunol. 12:714613. PubMed
  170. Dong M, et al. 2022. J Immunother Cancer. 10:. PubMed
  171. Yokota-Nakatsuma A, et al. 2016. Sci Rep. 6:37914. PubMed
  172. Park SR, et al. 2020. Cell Rep. 32:108077. PubMed
  173. Woo MS, et al. 2021. J Exp Med. :218. PubMed
  174. Chen RH, et al. 2021. Front Immunol. 11:594775. PubMed
  175. Gerson-Gurwitz A, et al. 2021. Front Oncol. 11:766298. PubMed
  176. Koutník J, et al. 2022. Cell Commun Signal. 20:54. PubMed
  177. Zhao X, et al. 2022. iScience. 25:104690. PubMed
  178. Cérbulo-Vázquez A, et al. 2022. PLoS One. 17:e0264566. PubMed
  179. Ansari M, et al. 2017. Cell Signal. 10.1016/j.cellsig.2017.04.014. PubMed
  180. Ahmad S, et al. 2017. Prog Neuropsychopharmacol Biol Psychiatry. 10.1016/j.pnpbp.2017.06.034. PubMed
  181. Glaser K, et al. 2016. PLoS One. 11: 0146898. PubMed
  182. DiMuzio JM, et al. 2021. Vaccine X. 8:100098. PubMed
  183. Lei L, et al. 2021. Front Cell Dev Biol. 659744:9. PubMed
  184. Frafjord A, et al. 2020. Scand J Immunol. e12889:92. PubMed
  185. Lownik JC, et al. 2020. Biochem Biophys Rep. 24:100803. PubMed
  186. Labi V, et al. 2019. Genes Dev. 33:1673. PubMed
  187. Rhoades NS, et al. 2022. Cell Rep. 39:110725. PubMed
  188. Tatangelo V, et al. 2022. Front Oncol. 12:877495. PubMed
  189. Huang T, et al. 2022. Nat Commun. 13:3489. PubMed
  190. Kissel T, et al. 2022. Sci Adv. 8:eabm1759. PubMed
  191. Deng Y, et al. 2021. Nat Commun. 12:7041. PubMed
  192. Haunerdinger V, et al. 2021. Front Immunol. 12:740047. PubMed
  193. Gensterblum E,et al. 2017. J Autoimmun.. 10.1016/j.jaut.2017.09.011. PubMed
  194. Zischke J, et al. 2017. PLoS Pathogens. 13(6):e1006454. PubMed
  195. Wang YJ, et al. 2021. Sci Rep. 11:8054. PubMed
  196. Wawrzyniak M, et al. 2021. Pharmacol Res Perspect. 9:e00837. PubMed
  197. Sun F, et al. 2022. Cell Death Dis. 13:181. PubMed
  198. Witt G, et al. 2020. Cell Biol Toxicol. . PubMed
  199. Sun Y, et al. 2022. iScience. 25:104846. PubMed
  200. Wang X, et al. 2022. Elife. 11:. PubMed
  201. Swarnalekha N, et al. 2021. Sci Immunol. 6:. PubMed
  202. Nouraei N,et al. 2017. Exp Neurol.. 10.1016/j.expneurol.2017.10.017. PubMed
  203. Marongiu L, et al. 2013. PLoS One. 8:75684. PubMed
  204. Zhang Z, et al. 2021. Front Immunol. 12:699478. PubMed
  205. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  206. Chen Y, et al. 2021. Braz J Med Biol Res. 54:e9570. PubMed
  207. Xiao C, et al. 2021. STAR Protoc. 2:100789. PubMed
  208. Melcher M, et al. 2017. Stem Cell Res Ther . 10.1186/s13287-017-0601-7. PubMed
  209. Clancy-Thompson E, et al. 2015. Cancer Immunol Res. 3: 956-967. PubMed
  210. Wang L, et al. 2016. Oncogene. 10.1038/onc.2016.380. PubMed
  211. Guo Z, et al. 2022. Neurobiol Pain. 12:100096. PubMed
  212. Yoshikawa AM, et al. 2021. Nat Commun. 12:7106. PubMed
  213. Nightingale K, et al. 2018. Cell Host Microbe. 24:447. PubMed
  214. Ikebuchi R, et al. 2016. Sci Rep. 6:35002. PubMed
  215. Liu Q, et al. 2016. Cell Death Dis. 1.93125. PubMed

Antigen Details

Antigen References

1. Current Protocols in Immunology (John Wiley & Sons New York) Unit 6.24 Detection of Intracellular Cytokines by Flow Cytometry (Barbara Foster and Calman Prussin NIAID NIH Bethesda MD).
2. Sander B, et al. 1991. Immunol. Rev. 119:65.
3. Sander B, et al. 1993. J. Immunol. Meth. 166:201.
4. Prussin C, et al. 1995. J. Immunol. Meth. 188:117.

Gene ID
NA

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 07/25/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account