Loading...

RBC Lysis Buffer (10X)

Pricing & Availability
Regulatory Status
RUO
Other Names
Red blood cell lysis buffer
Ave. Rating
Submit a Review
Product Citations
publications
Cat # Size Price Quantity Check Availability Save
420301 100 mL 52€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
420302 500 mL 165€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Red Blood Cell (RBC) Lysis Buffer has been designed, formulated, and tested to ensure optimal lysis of RBCs in single cell suspensions with minimal effects on leukocytes. RBC Lysis Buffer is supplied as a 10X solution containing ammonium chloride, potassium carbonate, and EDTA, and should be diluted in deionized water prior to use. Nucleated RBCs are not effectively lysed with ammonium chloride. Test

Product Details
Technical Data Sheet (pdf)

Product Details

Formulation
This red blood cell (RBC) lysis buffer is supplied as a 10X solution and should be diluted to 1X in deionized water. 100 ml of 10X concentrate will yield a quantity of 1X solution that is sufficient to lyse 500 samples.
Preparation
The pH of the 1X solution should fall within the range of pH 7.1-7.4. Adjust the pH if necessary. Warm the 1X solution to room temperature prior to use.
Storage & Handling
Store RBC lysis buffer between 2°C and 8°C.
Application Notes

A. Lysis of Mouse Spleen RBCs:
1. Harvest mouse spleen and prepare a single cell suspension.
2. Pellet the cells by centrifugation (350 x g); aspirate the supernatant.
3. Dilute the 10X Red Blood Cell Lysis Buffer to 1X working concentration with deionized water and resuspend the pellet in 5 ml of 1X Lysis Buffer.
4. Incubate on ice for 4-5 minutes with occasional shaking.
5. Stop the reaction by diluting the Lysis Buffer with 20-30 ml of 1X PBS.
6. Spin the cells (350 x g) and discard the supernatant.
7. Resuspend the pellet in the appropriate buffer (e.g., BioLegend Cell Staining Buffer Cat. No. 420201), wash 1X.
8. Count cells, adjust density, and proceed with cell staining procedures.
B. Lysis of Human Peripheral Blood RBCs:
1. Dilute the 10X RBC Lysis Buffer to 1X working concentration with deionized water. Warm the 1X solution to room temperature prior to use.
2. Add 2.0 ml of 1X RBC Lysis Buffer to each tube containing up to 100 µl of whole blood.
3. Gently vortex each tube immediately after adding the lysing solution. Incubate at room temperature, protected from light, for 10-15 minutes.
4. Centrifuge 350 x g for 5 minutes. Aspirate supernatant without disturbing pellet.
5. Resuspend the pellet in the appropriate buffer (e.g., BioLegend Cell Staining Buffer, Cat. No. 420201), wash 1X.
6. Resuspend and proceed with further procedures.

Application References
  1. Adams RA, et al. 2007. J. Exp. Med. 204:571.
  2. Feild-Corbett C, et al. 2009. Stem Cells. PubMed
  3. de Resende MM, et al. 2010. Physiol Genomics. 42:437. PubMed
  4. Roybal JD, et al. 2011. Mol Cancer Res. 9:25. PubMed
  5. Boldin MP, et al. 2011. J Exp Med. PubMed
  6. Kahlenberg JM, et al. 2011. J. Immunol. 187:6143. PubMed
  7. Deiuliis JA, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 302:399. PubMed
  8. Wu CY, et al. 2012. PLoS One. 7:e42365. PubMed
  9. Hsia BJ, et al. 2012. J Allergy Clin Immunol. 130:205. PubMed
  10. Rico C, et al. 2012. Carcinogenesis. 33:2283. PubMed
  11. Jin J, et al. 2012. Arterioscler Thromb Vasc Biol. 32:2901. PubMed
  12. Saraiva AM, 2013. Hum Immunol. 74:207. PubMed
  13. Koon HW, et al. 2013. J Vis exp. 68:4208. PubMed
  14. Ma Dy, et al. 2013. J Virol. 87:3361. PubMed
  15. Han W, 2013. J. Immunol. 190:4786. PubMed
  16. Garcia-Acros I, et al. 2013. J Biol Chem. 288:14046. PubMed
  17. Zhao JL, et al. 2013. Elife. 21:537. PubMed
  18. Guo W, et al. 2013. J Gerontol A Biol Sci Med Sci. PubMed
  19. Yamasaki S, et al. 2013. Clin Vaccine immunol. 20:1508. PubMed
  20. Kraft P, et al. 2013. Stroke. 44:3202. PubMed
  21. Liu X, et al. 2013. Invest Ophthalmol Vis Sci. 54:7386. PubMed
  22. Fullerton AM, et al. 2013. Toxicol Sci. 136:72. PubMed
  23. Cekic C, et al. 2013. J Exp Med. 210:2693. PubMed
  24. Varga T, et al. 2013. J. Immunol. 191:5695. PubMed
  25. Ma Y, et al. 2014. Cancer Res. 74:436. PubMed
  26. Chang YJ, et al. 2014. Cytotherapy. 16:560. PubMed
  27. Kim MJ, et al. 2014. Mol Pharmacol. 86:284. PubMed
  28. Cekic C, et al. 2014. Cancer Res. 74:7250. PubMed
  29. Szulzewsky F, et al. 2015. PloS One. 10:116644. PubMed
Product Citations
  1. Rathakrishnan A, et al. 2020. Viruses. 12: . PubMed
  2. Wang Y, et al. 2021. Cancer Res. 81:384. PubMed
  3. Wang H, et al. 2022. Nat Biomed Eng. 6:882. PubMed
  4. Yeh PY, et al. 2022. Sens Actuators B Chem. 353: . PubMed
  5. Ammothumkandy A, et al. 2022. Nat Neurosci. 25:493. PubMed
  6. Melendez E, et al. 2022. Development. 149: . PubMed
  7. Argueta LB, et al. 2022. iScience. 25:104223. PubMed
  8. Wang S, et al. 2022. Adv Sci (Weinh). 9:e2201258. PubMed
  9. Meena AS, et al. 2022. Cell Rep. 39:110937. PubMed
  10. Zhang Y, et al. 2022. Vaccines (Basel). 10: . PubMed
  11. Sugimoto S, et al. 2022. Nat Metab. 4:775. PubMed
  12. Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed
  13. Isildar B, et al. 2022. Inflamm Regen. 42:55. PubMed
  14. Xu Q, et al. 2022. Front Med (Lausanne). 9:940554. PubMed
  15. Alexandrou AT, et al. 2022. iScience. 25:105546. PubMed
  16. Wang Y, et al. 2022. Front Endocrinol (Lausanne). 13:1036517. PubMed
  17. He Y, et al. 2022. Front Immunol. 13:986202. PubMed
  18. Tratwal J, et al. 2022. Front Endocrinol (Lausanne). 13:1001210. PubMed
  19. Xie Z, et al. 2022. Cell Discov. 8:133. PubMed
  20. Xiang X, et al. 2022. Medicine (Baltimore). 101:e32558. PubMed
  21. Yu Z, et al. 2023. Cancer Res. 83:700. PubMed
  22. Ding D, et al. 2023. J Med Chem. 66:1467. PubMed
  23. Oh S, et al. 2023. Nat Biotechnol. :. PubMed
  24. Marritt KL, et al. 2023. Front Immunol. 13:1087991. PubMed
  25. He T, et al. 2023. Immun Inflamm Dis. 11:e759. PubMed
  26. Vyas M, et al. 2023. Front Immunol. 13:1098445. PubMed
  27. Li XF, et al. 2023. J Exp Med. 220: . PubMed
  28. Wang J, et al. 2023. Inflamm Regen. 43:12. PubMed
  29. Ma Y, et al. 2022. Am J Physiol Heart Circ Physiol. 322:H622. PubMed
  30. Feng J, et al. 2022. Immunity. 55:405. PubMed
  31. Rustagi Y, et al. 2022. Diabetes. 71:1149. PubMed
  32. Guennoun R, et al. 2022. Cancers (Basel). 14:. PubMed
  33. Zhuo Y, et al. 2022. J Immunother Cancer. 10:. PubMed
  34. Knelson EH, et al. 2022. Cancer Immunol Res. 10:947. PubMed
  35. Wakiyama H, et al. 2022. Cancer Immunol Res. 10:1386. PubMed
  36. Roweth HG, et al. 2022. Sci Adv. 8:eabo5224. PubMed
  37. Zhong J, et al. 2022. J Adv Res. 41:145. PubMed
  38. Yun HJ, et al. 2022. Int J Mol Sci. 23:. PubMed
  39. Daum P, et al. 2023. Front Immunol. 13:991347. PubMed
  40. Rao D, et al. 2023. Eur J Immunol. 53:e2250258. PubMed
  41. Le DT, et al. 2023. iScience. 26:106059. PubMed
  42. Romo-Rodríguez R, et al. 2023. Arch Med Res. 54:197. PubMed
  43. Mabire M, et al. 2023. Nat Commun. 14:1830. PubMed
  44. MacPherson KP, et al. 2023. Front Cell Neurosci. 17:895017. PubMed
  45. Chen Z, et al. 2023. Nat Commun. 14:1839. PubMed
  46. Martins Nascentes Melo L, et al. 2023. J Immunother Cancer. 11:. PubMed
  47. Ramalingam P, et al. 2023. Nat Commun. 14:2018. PubMed
  48. Choi YJ, et al. 2023. Nat Commun. 14:2045. PubMed
  49. He Q, et al. 2023. Parasit Vectors. 16:139. PubMed
  50. Pattnaik A, et al. 2023. Vaccines (Basel). 11:. PubMed
  51. Mulcrone PL, et al. 2023. Sci Rep. 13:8436. PubMed
  52. Villalba N, et al. 2023. J Neuroinflammation. 20:127. PubMed
  53. Song S, et al. 2023. Bioact Mater. 28:358. PubMed
  54. Matsudaira T, et al. 2023. Commun Biol. 6:665. PubMed
  55. Dobie R, et al. 2020. Cell Reports. 29(7):1832-1847.e8.. PubMed
  56. Strickley JD, et al. 2019. Nature. 575:519. PubMed
  57. Deiuliis J, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 302:L399. PubMed
  58. Jin J, et al. 2012. Arterioscler Thromb Vasc Biol. 32:2901. . PubMed
  59. Sandovici I, et al. 2022. Dev Cell. 57:63. PubMed
  60. Zhou B, et al. 2020. Nat Immunol. 1.868055556. PubMed
  61. Tan J, et al. 2021. iScience. 24(8):102835. PubMed
  62. Asem M, et al. 2020. Cancer Res. 1156:80. PubMed
  63. Lin C, et al. 2020. Cancer Immunol Res. 632:8. PubMed
  64. Tsai AG, et al. 2020. Nat Med. 408:26. PubMed
  65. Shah D, et al. 2021. Oncoimmunology. 10:1939601. PubMed
  66. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  67. Barros-Martins J, et al. 2016. J Immunol. 196: 3642 - 3652. PubMed
  68. Pracht K, et al. 2021. Eur J Immunol. 51:1089. PubMed
  69. Radwanska M, et al. 2020. Methods Mol Biol. 2116:721. PubMed
  70. Rendon CJ, et al. 2022. Front Endocrinol (Lausanne). 13:995499. PubMed
  71. Effern M, et al. 2022. STAR Protoc. 3:101038. PubMed
  72. Della Verde G, et al. 2022. Life Sci Alliance. 5:. PubMed
  73. Su C, et al. 2021. Front Oncol. 11:719564. PubMed
  74. Herisson F, et al. 2018. Nat Neurosci. 21:1209. PubMed
  75. Jacoby E, et al. 2021. NPJ Regen Med. 6:58. PubMed
  76. Chen Z, et al. 2020. Glia. 68:2148. PubMed
  77. Seow JJW, et al. 2021. Front Med (Lausanne). 8:603374. PubMed
  78. Shin J, et al. 2020. Hum Gene Ther. . PubMed
  79. Bouchareychas L, et al. 2021. iScience. 24(8):102847. PubMed
  80. Spengler J, et al. 2016. J Infect Dis. 214: S308 - S318. PubMed
  81. Zheng M, et al. 2016. J Immunol. 196: 4122 - 4131. PubMed
  82. St-Pierre S, et al. 2016. PLoS One. 11: 0150230. PubMed
  83. Ensan S, et al. 2015. Nat Immunol. 17: 159-168. PubMed
  84. Jaiswal M, et al. 2015. Sci Rep. 5: 15221. PubMed
  85. Hou J, et al. 2015. J Mol Cell Biol. 7: 338-350. PubMed
  86. Fujii H, et al. 2015. PLoS One. 10: 0133216. PubMed
  87. Bikorimana JP, et al. 2022. Cell Rep Med. 3:100534. PubMed
  88. Zhang L, et al. 2022. JCI Insight. 7:. PubMed
  89. Baldominos P, et al. 2022. STAR Protoc. 3:101795. PubMed
  90. Chou YJ, et al. 2020. Sci Rep. 10:8422. PubMed
  91. Wang J, et al. 2020. Sci Rep. 10:12785. PubMed
  92. Ma H, et al. 2021. Front Immunol. 12:760095. PubMed
  93. McGill CJ, et al. 2021. STAR Protoc. 2:100948. PubMed
  94. Lutze J, et al. 2022. Methods Mol Biol. 2394:299. PubMed
  95. Hosseini A, et al. 2022. Evid Based Complement Alternat Med. 2022:5639226. PubMed
  96. Liu W, et al. 2017. Int J Environ Res Public Health. 10.3390/ijerph14060592. PubMed
  97. Pietrocola F, et al. 2017. Autophagy. 0.935416667. PubMed
  98. Garcia-Fabiani MB, et al. 2020. STAR Protoc. :1. PubMed
  99. Zhang J, et al. 2021. Aging (Albany NY). 13:17880. PubMed
  100. Press AT, et al. 2021. EMBO Mol Med. 13:e14436. PubMed
  101. Sima LE, et al. 2021. J Immunother Cancer. 9:. PubMed
  102. Ma Y, et al. 2014. Cancer Res. 74:436. PubMed
  103. Magalhães L, et al. 2015. PLoS Negl Trop Dis. 10: 0003816. PubMed
  104. Moraes-Vieira P, et al. 2016. Diabetes. 65: 1317 - 1327. PubMed
  105. Chen J, et al. 2022. Nat Commun. 13:6759. PubMed
  106. Boldin M, et al. 2011. J Exp Med. 208:1189. PubMed
  107. Sun CC, et al. 2020. Genome Med. 0.553472222. PubMed
  108. Bravo–San Pedro JM, et al. 2019. Cell Metab. 30:754. PubMed
  109. Wu Y, et al. 2021. Nat Biomed Eng. 5:1336. PubMed
  110. Meng S, et al. 2022. Exp Anim. . PubMed
  111. Kokkinopoulos I, et al. 2021. Sci Rep. 11:15759. PubMed
  112. Clark EL, et al. 2017. PLoS Genet. 13:e1006997. PubMed
  113. Cantor JR, et al. 2017. Cell. 169:258. PubMed
  114. Ji WJ, et al. 2017. Cytometry B Clin Cytom. 92:371. PubMed
  115. Johnson CL, et al. 2021. Am J Physiol Heart Circ Physiol. 321:H756. PubMed
  116. Stringhini M, et al. 2021. MAbs. 13:1868066. PubMed
  117. Devalaraja S, et al. 2020. Cell. 1098:180. PubMed
  118. Elisabeth Schrumpf, Xiaojun Jiang 2017. Physiol Rep. 10.14814/phy2.13117. PubMed
  119. AC Belkina, JE Snyder-Cappione 2017. Cytometry A. 91:175-179. PubMed
  120. Brunmeir R, et al. 2016. PLoS Genet. 12:e1006474. PubMed
  121. Pridans C, et al. 2016. J Immunol. 197: 2297 - 2305. PubMed
  122. Mircheff A, et al. 2016. Ocul Surf. 14:460-483. PubMed
  123. Pennington L, et al. 2016. Nat Commun. 7:11610. PubMed
  124. Koon H, et al. 2012. J Vis Exp. 68: 4208. PubMed
  125. Pokhrel RH, et al. 2022. Int J Mol Sci. 23:. PubMed
  126. Sakamoto S, et al. 2020. OncoImmunology. 8(12):e1671760.. PubMed
  127. Rico C, et al. 2012. Carcinogenesis. 33:2283. PubMed
  128. Wang D, et al. 2021. Signal Transduct Target Ther. 6:376. PubMed
  129. Machado H, et al. 2021. PLoS Pathog. 17:e1009933. PubMed
  130. Yan X, et al. 2022. Int J Biol Sci. 18:585. PubMed
  131. Singh SS, et al. 2021. Eur J Med Chem. 216:113312. PubMed
  132. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  133. Zhai Z, et al. 2021. Medicine (Baltimore). 100:e26214. PubMed
  134. Hu D, et al. 2021. J Cell Mol Med. 25:2900. PubMed
  135. Jones N, et al. 2021. Nat Commun. 12:1209. PubMed
  136. Hamour S, et al. 2015. PLoS One. 10: 0136238. PubMed
  137. Bazett M, Haston M 2016. Sci Rep. 6:19189. PubMed
  138. Luo B, et al. 2016. J Neurosci. 36: 9590 - 9603. PubMed
  139. Patera A, et al. 2016. J Leukoc Biol. 100(6):1239-1254. PubMed
  140. Mo L, et al. 2022. Front Oncol. 12:871489. PubMed
  141. Saraiva A, et al. 2013. Hum Immunol. 74:207. PubMed
  142. Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed
  143. Huang L, et al. 2021. J Cell Physiol. 236:7308. PubMed
  144. Whytock KL, et al. 2022. iScience. 25:104772. PubMed
  145. Neufeld L, et al. 2021. Sci Adv. 7:. PubMed
  146. Friedman G, et al. 2020. Nat Cancer. 1:692. PubMed
  147. Kumar A, et al. 2021. Part Fibre Toxicol. 18:43. PubMed
  148. Tanaka Y, et al. 2021. Int J Mol Sci. 22:. PubMed
  149. Scherer SD, et al. 2021. Breast Cancer Res. 23:100. PubMed
  150. Jiang J, et al. 2018. Cancer Med. 3.504861111. PubMed
  151. Chen Z, et al. 2017. Cancer Res. 77:2266. PubMed
  152. Kim M, et al. 2017. Int J Mol Sci. 10.3390/ijms18061275. PubMed
  153. Jeong J, et al. 2021. Immune Netw. 21:e31. PubMed
  154. Sitaraman S, et al. 2021. JCI Insight. 6:. PubMed
  155. Orr AN, et al. 2021. Int J Mol Sci. 22:. PubMed
  156. Facchini M, et al. 2014. J Vis Exp. 85: 51019. PubMed
  157. Ilievski V, et al. 2015. PLoS One. 10: 0136502. PubMed
  158. Qin L, et al. 2022. Nat Commun. 13:6051. PubMed
  159. Escudero-Lara A, et al. 2022. Front Behav Neurosci. 16:946975. PubMed
  160. Patil R, et al. 2019. J Biomed Opt. 24:1. PubMed
  161. Ongaro T, et al. 2020. Oncotarget. 3.026388889. PubMed
  162. Kahlenberg J, et al. 2011. J Immunol. 187:6143. PubMed
  163. Han W, et al. 2013. J Immunol. 190:4786. PubMed
  164. Dang Y, et al. 2022. Cancer Cell Int. 22:88. PubMed
  165. Deng J, et al. 2021. Breast Cancer Res Treat. 185:85. PubMed
  166. Vivanco Gonzalez N, et al. 2022. STAR Protoc. 3:101280. PubMed
  167. Wang K, et al. 2017. Molecular Immunology. 10.1016/j.molimm.2017.04.016. PubMed
  168. Appelbe O, et al. 2017. J Control Release.. 10.1016/j.jconrel.2017.09.043. PubMed
  169. Tang J, et al. 2017. Proc Natl Acad Sci U S A. 114:E7441. PubMed
  170. Liccardi G, et al. 2019. Mol Cell. 73:413. PubMed
  171. Kildisiute G, et al. 2021. Sci Adv. 7: . PubMed
  172. Schiferle EB, et al. 2021. Sci Adv. 7: . PubMed
  173. Devalaraja S, et al. 2020. STAR Protoc. 1:100188. PubMed
  174. Bigenwald C, et al. 2021. Nat Med. 27:851. PubMed
  175. Gingrich AA, et al. 2021. Front Immunol. 12:670309. PubMed
  176. Yamasaki S, et al. 2013. Clin Vaccine Immunol. 20:1508. PubMed
  177. Fukui R, et al. 2016. J Immunol. 196: 416 - 427. PubMed
  178. Rodriguez-Garcia M, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.72. PubMed
  179. Martínez-Muriana A, et al. 2020. Stem Cell Res Ther. 11:53. PubMed
  180. Pennig J, et al. 2019. Sci Rep. 9:17937. PubMed
  181. Zhang L, et al. 2021. Methods Mol Biol. 2388:175. PubMed
  182. Evans AK, et al. 2020. Neurobiol Dis. 146:105089. PubMed
  183. Wang B, et al. 2017. Stem Cell Res Ther.. 10.1186/s13287-017-0744-6. PubMed
  184. Anstine L,et al. 2017. J Am Heart Assoc.. 10.1161/JAHA.117.007097. PubMed
  185. Judge SJ, et al. 2020. J Clin Invest. 130:3051. PubMed
  186. Choi JW, et al. 2020. PLoS One. 15:e0235518. PubMed
  187. Alban TJ, et al. 2020. Front Immunol. 1.285416667. PubMed
  188. Jiang S, et al. 2017. Toxicology Lett. 10.1016/j.toxlet.2017.03.015. PubMed
  189. Agrawal V, et al. 2016. J Leukoc Biol. 100: 261 - 274. PubMed
  190. Nwani NG, et al. 2019. Cancers (Basel). 0.806944444. PubMed
  191. Ramalingam P, et al. 2020. Nat Commun. 11:666. PubMed
  192. Garcia-Arcos I, et al. 2013. J Biol Chem. 288:14046. PubMed
  193. Beaudoin-Bussières G, et al. 2022. Cell Rep. 38:110368. PubMed
  194. Hu H, et al. 2021. Cancer Immunol Res. 9:1298. PubMed
  195. Zhong W, et al. 2022. Nat Commun. 13:4390. PubMed
  196. So EY, et al. 2021. Am J Physiol Cell Physiol. 321:C569. PubMed
  197. Bunting MD, et al. 2022. Sci Adv. 8:eabk3327. PubMed
  198. Elphinstone R, et al. 2017. Infect Immun. 10.1128/IAI.00371-17. PubMed
  199. Kernfeld EM, et al. 2018. Immunity. 48:1258. PubMed
  200. Cheng Q, et al. 2020. Nat Nanotechnol. 0.842361111. PubMed
  201. Xie Z, et al. 2020. Light Sci Appl. 0.486805556. PubMed
  202. Alghamri MS, et al. 2020. Methods Enzymol. 632:215. PubMed
  203. Yu Z, et al. 2021. Front Oncol. 11:659251. PubMed
  204. Deng J, et al. 2021. Nat Cancer. 2:503. PubMed
  205. Sci T 2013. Toxicol Sci. 136:72. PubMed
  206. Metz H, et al. 2016. Proc Natl Acad Sci U S A. 113: 8795 - 8800. PubMed
  207. Mirando AC, et al. 2020. Oncoimmunology. 9:1760685. PubMed
  208. Rosenbluth JM, et al. 2020. Nat Commun. 11:1711. PubMed
  209. Tavakol DN, et al. 2021. Curr Protoc. 1:e275. PubMed
  210. Ullah I, et al. 2021. Immunity. 54:2143. PubMed
  211. He Y, et al. 2021. Front Immunol. 12:641206. PubMed
  212. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  213. Ercoli G, et al. 2022. Clin Transl Immunology. 11:e1366. PubMed
  214. Meng YM, et al. 2021. Nat Commun. 12:6011. PubMed
  215. Zhu QY, et al. 2021. Nat Commun. 12:6624. PubMed
  216. Patel M, et al. 2021. Cancer Res. 81:3985. PubMed
  217. Shi X, et al. 2021. Nat Commun. 12:2547. PubMed
  218. Ramaswamy G, et al. 2017. Sci Rep. 10.1038/srep45140. PubMed
  219. Lizotte P, et al. 2016. Sci Rep. 6:31745. PubMed
  220. Fidalgo MF, et al. 2022. Life Sci Alliance. 5:. PubMed
  221. Stirling ER, et al. 2022. J Immunother Cancer. 10:. PubMed
  222. Hang J, et al. 2019. Cancer Med. 1.254166667. PubMed
  223. Tan X, et al. 2019. Sci Rep. 2.7125. PubMed
  224. Lienemann PS, et al. 2020. Adv Sci (Weinh). 7:1903395. PubMed
  225. Zhao J, et al. 2013. Elife. 21:537. PubMed
  226. Hills LB, et al. 2021. J Immunol. 206:89. PubMed
  227. Page N, et al. 2021. Nat Commun. 12:1009. PubMed
  228. Pace J, et al. 2022. J Biomed Opt. 27:. PubMed
  229. Pedicona F, et al. 2022. Sci Signal. 15:eabl7989. PubMed
  230. Wen J, et al. 2017. J Hematol Oncol. 10.1186/s13045-017-0489-9. PubMed
  231. Ahmad S,et al. 2017. Prog Neuropsychopharmacol Biol Psychiatry. . 10.1016/j.pnpbp.2017.10.001. PubMed
  232. Sharma A, et al. 2020. Cell. 183(2):377-394.e21. PubMed
  233. Weber G, et al. 2015. Surgery. 158: 248-254. PubMed
  234. Jiang H, et al. 2016. Nat Med. 10.1038/nm.4123. PubMed
  235. Elgueta R, et al. 2016. J Immunol. 197: 3970 - 3981. PubMed
  236. Yan Q, et al. 2022. Clin Transl Med. 12:e1084. PubMed
  237. Schweppe RE, et al. 2019. Mol Cancer Res. 17:1036. PubMed
  238. Yamada KJ, et al. 2020. PLoS Pathog. 16:e1008354. PubMed
  239. Zhai K, et al. 2021. Nat Cancer. 2:1136. PubMed
  240. Yang A, et al. 2018. Cancer Discov. 0.525. PubMed
  241. Rentsendorj A, et al. 2017. Brain Behav Immun. 10.1016/j.bbi.2017.08.019. PubMed
  242. Zhao D, et al. 2020. Cancer Discov. 10:1374. PubMed
  243. Castoldi F, et al. 2020. Cell Death Discov. 0.339583333. PubMed
  244. Wang L, et al. 2020. EMBO J. 39:e104514. PubMed
  245. Morris C, et al. 2017. MBio. 10.1128/mBio.00036-17. PubMed
  246. Theurl I, et al. 2016. Nat Med. 10.1038/nm.4146. PubMed
  247. Michelotti TC, et al. 2022. Front Cell Dev Biol. 10:1025240. PubMed
  248. Ren X, et al. 2022. STAR Protoc. 3:101818. PubMed
  249. Yang YS, et al. 2020. Mol Ther Methods Clin Dev. 17:922. PubMed
  250. Gangwar RS, et al. 2020. Sci Rep. 10:14348. PubMed
  251. Hollerbach A, et al. 2021. J Thromb Haemost. . PubMed
  252. Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed
  253. Yang Z, et al. 2021. Nat Commun. 12:4852. PubMed
  254. Zhang Y, et al. 2022. Vaccines (Basel). 10: . PubMed
  255. Majumdar D, et al. 2021. Curr Protoc. 1:e216. PubMed
  256. Dyck S, et al. 2018. J Neuroinflammation. 0.6875. PubMed
  257. Nowyhed H, et al. 2017. Sci Rep. 7:40273. PubMed
  258. De Domenico E, et al. 2020. STAR Protoc. 1:100233. PubMed
  259. Wang Z, et al. 2020. Cell Rep. 33:108472. PubMed
  260. Sadik A, et al. 2020. Cell. 1252:182. PubMed
  261. Hiza H, et al. 2021. Sci Rep. 11:13190. PubMed
  262. Ito T, et al. 2021. Nat Commun. 12:2232. PubMed
  263. Varga T, et al. 2013. J Immunol. 191:5695. PubMed
  264. Samuelson D, et al. 2016. J Immunol. 196: 2655 - 2665. PubMed
  265. Tsao P, et al. 2016. Proc Natl Acad Sci U S A. 113: 8242 - 8247. PubMed
  266. Soncini M, et al. 2016. Proc Natl Acad Sci U S A. 113: E6219 - E6227. PubMed
  267. Yang C, et al. 2016. Sci Rep. 6:34643. PubMed
  268. Feng D, et al. 2022. Nat Commun. 13:5554. PubMed
  269. Gladow N, et al. 2020. PLoS One. 15:e0227734. PubMed
  270. Ramachandran P, et al. 2019. Nature. 575:512. PubMed
  271. Saber M, et al. 2021. J Neurosci Res. 99:1136. PubMed
  272. Xiang Y, et al. 2022. Skelet Muscle. 12:19. PubMed
  273. Yamazaki T, et al. 2021. Oncoimmunology. 10:1962592. PubMed
  274. Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed
  275. Bowers J, et al. 2017. Front Immunol. 10.3389/fimmu.2017.01221. PubMed
  276. Zhang J, et al. 2020. Pain. 161:1381. PubMed
  277. Heim CE, et al. 2020. Nature Microbiology. 5(10):1271-1284. PubMed
  278. Canali S, et al. 2017. Blood. 129(4):405-414. PubMed
  279. Shen X, et al. 2016. Sci Rep. 6:38778. PubMed
  280. Sauter K, et al. 2016. Am J Physiol Gastrointest Liver Physiol. 311: G533 - G547. PubMed
  281. Song C, et al. 2015. Blood. 126: 1813 - 1822. PubMed
  282. Sasaki Y, et al. 2015. PLoS One. 10: 0131176. PubMed
  283. Yang YS, et al. 2022. Nat Commun. 13:6175. PubMed
  284. Ma D, et al. 2013. J Virol. 87:3361. PubMed
  285. Xue X, et al. 2021. Cent Eur J Immunol. 46:191. PubMed
  286. Unsinger J, et al. 2021. J Leukoc Biol. 109:697. PubMed
  287. Ansari M, et al. 2017. Cell Signal. 10.1016/j.cellsig.2017.04.014. PubMed
  288. Ahmad S, et al. 2017. Prog Neuropsychopharmacol Biol Psychiatry. 10.1016/j.pnpbp.2017.06.034. PubMed
  289. Bichsel C, et al. 2017. Sci Rep. 10.1038/s41598-017-09928-1. PubMed
  290. James M, et al. 2017. J Nucl Med. . 10.2967/jnumed.117.189597. PubMed
  291. Annibaldi A, et al. 2018. Mol Cell. 69:566. PubMed
  292. Hill SJ, et al. 2018. Cancer Discov. 1.308333333. PubMed
  293. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  294. Tulasi DY, et al. 2021. Cell Mol Gastroenterol Hepatol. 11:1437. PubMed
  295. Hoffmann J, et al. 2021. Nat Commun. 12:3964. PubMed
  296. Kraft P, et al. 2013. Stroke. 44:3202. PubMed
  297. Kim M, et al. 2014. Mol Pharmacol. 86:284. PubMed
  298. Cekic C, et al. 2014. Cancer Res. 74:7250. PubMed
  299. Pankova D, et al. 2016. Mol Cancer Res. 14: 287 - 295. PubMed
  300. Li N, et al. 2022. Nat Commun. 13:7281. PubMed
  301. Hsia B, et al. 2012. J Allergy Clin Immunol. 130:205. PubMed
  302. Resende M, et al. 2010. Physiol Genomics. 42:437. PubMed
  303. McLaughlin S, et al. 2019. Nat Commun. 3.795833333. PubMed
  304. Koury J, et al. 2019. PLoS One. 14:e0218670. PubMed
  305. Soileau AM, et al. 2022. Vet Sci. 9:. PubMed
  306. McClellan BL, et al. 2022. STAR Protoc. 3:101357. PubMed
  307. Governa V, et al. 2022. Proc Natl Acad Sci U S A. 119. PubMed
  308. Deng Y, et al. 2021. Nat Commun. 12:7041. PubMed
  309. Zhang Z, et al. 2017. Journal of Translational Medicine. 10.1186/s12967-017-1196-6. PubMed
  310. Mai CL, et al. 2021. Theranostics. 11:1059. PubMed
  311. Chen P, et al. 2021. Clin Transl Med. 11:e462. PubMed
  312. Peng X, et al. 2021. Stem Cell Res Ther. 198:12. PubMed
  313. Linder A, et al. 2020. EMBO J. 39:e105071. PubMed
  314. Muñoz-Ruiz M, et al. 2016. Nat Immunol. 10.1038/ni.3424. PubMed
  315. Chang Y, et al. 2014. Cytotherapy. 16:560. PubMed
  316. Sobecki M, et al. 2022. Cell Stem Cell. 29:1459. PubMed
  317. Cordo' V, et al. 2022. Nat Commun. 13:1048. PubMed
  318. Hallisey M, et al. 2022. STAR Protoc. 3:101163. PubMed
  319. Denoth-Lippuner A, et al. 2021. Cell Stem Cell. 28:2020. PubMed
  320. Maas RR, et al. 2021. Nat Protoc. 16:4692. PubMed
  321. Nagree MS, et al. 2022. EMBO Mol Med. 14:e14297. PubMed
  322. Mochizuki M,et al. 2017. J Am Heart Assoc. . 10.1161/JAHA.117.005920. PubMed
  323. Roh–Johnson M, et al. 2017. Dev Cell. 43:549. PubMed
  324. Müller C, et al. 2018. Elife. 7:e34985. PubMed
  325. Ubellacker J, et al. 2017. Breast Cancer Res. 10.1186/s13058-017-0815-8. PubMed
  326. DU T, et al. 2020. Front Immunol. 1.571527778. PubMed
  327. Trial J, et al. 2020. Geroscience. . PubMed
  328. Guo W, et al. 2013. J Gerontol A Biol Sci Med Sci. . PubMed
  329. Liu Y, et al. 2015. Sci Rep. 5: 15134. PubMed
  330. Hodson D, et al. 2016. Proc Natl Acad Sci U S A. 113: 2039-2046. PubMed
  331. Munson D, et al. 2016. Proc Natl Acad Sci U S A. 113: 8272 - 8277. PubMed
  332. Visweshwaran SP, et al. 2022. Front Cell Dev Biol. 10:1008898. PubMed
  333. Wu C, et al. 2012. PLoS One. 7:e42363. PubMed
  334. Denning W, et al. 2011. PLoS One. 6:e16897. PubMed
  335. Li Q, et al. 2022. Signal Transduct Target Ther. 7:222. PubMed
  336. Fielding C, et al. 2022. Nat Commun. 13:543. PubMed
  337. Weaver J, et al. 2017. Part Fibre Toxicol. 10.1186/s12989-017-0206-4. PubMed
  338. Samuelson D, et al. 2017. PLoS Pathogens. 13(6):e1006426. PubMed
  339. Wong C, et al. 2017. J Immunol. 10.4049/jimmunol.1700397. PubMed
  340. Vechetti IJ, et al. 2021. FASEB J. 35:e21644. PubMed
  341. Saber M, et al. 2020. Eur J Neurosci. 52:2791. PubMed
  342. Wu R, et al. 2020. J Immunother Cancer. 8:00. PubMed
  343. Yu H, et al. 2016. Sci Rep. 6:35190. PubMed
  344. Now H, Yoo J 2016. Cell Mol Immunol. 10.1038/cmi.2016.32. PubMed
  345. Bosma M, et al. 2016. Nat Commun. 7: 11314. PubMed
  346. YC S, SJ L 2015. Sci Rep. 5: 12578. PubMed
  347. Szulzewsky F, et al. 2015. PLoS One. 10:116644. PubMed
  348. Shaashua L, et al. 2022. Nat Commun. 13:6513. PubMed
  349. Gupta R, et al. 2020. Cell Stem Cell. 26:527. PubMed
  350. Roybal J, et al. 2011. Mol Cancer Res. 9:25. PubMed
  351. Yeini E, et al. 2021. Nat Commun. 12:1912. PubMed
  352. Wang X, et al. 2021. Sci Transl Med. 13:. PubMed
  353. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  354. Kucykowicz S, et al. 2022. STAR Protoc. 3:101356. PubMed
  355. Kim TW, et al. 2022. Mol Ther Oncolytics. 26:1. PubMed
  356. Kathania M, et al. 2022. Cell Rep. 40:111345. PubMed
  357. Barcia Durán JG, et al. 2021. Commun Biol. 4:406. PubMed
  358. Park HJ, et al. 2021. Int J Mol Sci. 22:. PubMed
  359. Cebrian-Silla A, et al. 2021. Elife. 10:. PubMed
  360. Cekic C, et al. 2013. J Exp Med. 210:2693. PubMed
  361. Field-Corbett C, et al. 2009. Stem Cells. 27:662. PubMed
  362. Zeng Y, et al. 2019. FASEB J. 33:6596. PubMed
  363. Zheng W, et al. 2019. Cell Rep. 29:1747. PubMed
  364. Tomas C, et al. 2019. Sci Rep. 9:11464. PubMed
  365. Kim JK, et al. 2019. Bioengineering (Basel). 6:24. PubMed
  366. Roy IM, et al. 2021. Bio Protoc. 11:e4130. PubMed
  367. Alam A, et al. 2022. STAR Protoc. 3:101563. PubMed
  368. Xiang Y, et al. 2022. Exp Mol Med. 54:1011. PubMed
  369. Guo Z, et al. 2022. Neurobiol Pain. 12:100096. PubMed
  370. Guo P, et al. 2022. Nat Cell Biol. 24:99. PubMed
  371. Wahane S, et al. 2021. Sci Adv. 7:. PubMed
  372. Alizadeh A, et al. 2018. J Neuroinflammation. 15:53. PubMed
  373. Guo P, et al. 2017. J Clin Invest. 127:4242. PubMed
  374. Tsai SC, et al. 2017. Biomicrofluidics. 11:034122. PubMed
  375. Xie Z, et al. 2020. Light Sci Appl. 9:161. PubMed
  376. Huang N, et al. 2021. Nat Med. 27:892. PubMed
  377. Song X, et al. 2021. FEBS Open Bio. 11:2453. PubMed
  378. Chen S, et al. 2020. Cancer Immunol Res. 1064:8. PubMed
  379. Erady C, et al. 2021. NPJ Genom Med. 6:04. PubMed
  380. de Silva S, et al. 2020. Cancer Immunol Res. 0.493055556. PubMed
  381. Wan C, et al. 2021. Cancer Res. 81:158. PubMed
  382. Wang X, et al. 2016. J Virol. 90: 10247 - 10258. PubMed
  383. Mohammed R, et al. 2016. Cell Rep. 14:760-771. PubMed
  384. Durward-Diioia M, et al. 2015. Infect Immun . 83: 4759 - 4771. PubMed
  385. Birnie K, et al. 2015. Mol Cancer Res. 13: 1106 - 1118. PubMed
  386. Liu X, et al. 2013. Invest Ophthalmol Vis Sci . 54:7386. PubMed

Antigen Details

Cell Type
Tregs
Gene ID
NA

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 06/10/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account