StarBright UltraViolet 740 anti-mouse Ly-6C antibody, Ly-6C, HK1.4

Pricing & Availability

Clone: HK1.4 (See other available formats)
Regulatory Status: RUO
Other Names: Lymphocyte antigen 6 complex, locus C
Isotype: Rat IgG2c, κ
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Product Citations: publications

HK14_SBUV740_Ly-6C_FC_090225 — C57BL/6 mouse splenocytes were stained with anti-mouse CD8a (clone 53-6.7) APC and anti-mouse Ly-6C (clone HK1.4) StarBright UltraViolet 740 (left) or stained with anti-mouse CD8a (clone 53-6.7) only (right).

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128069

150 μg

245€

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This StarBright conjugate is available for purchase as part of a multicolor flow cytometry panel. To order, add compatible products to your shopping cart, or use our Panel Selector tool to build your ideal panel. For ordering assistance or to request a sample, contact your local field representative.

This product is also available for direct purchase within a StarBright Basic Panel, which combines a minimal set of pre-selected compatible reagents.

Description

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8⁺ T cells.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: L3 cloned CTL cells
Formulation: Phosphate-buffered saline, containing 0.09% sodium azide, 0.05% Tween 20, 0.1% PEG 3350, 0.1% Pluronic F68, and 1% BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with StarBright UltraViolet 740 under optimal conditions.
Concentration: 0.6 mg/mL (the concentration relates to the dye component of the conjugate)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* StarBright UltraViolet 740 has a maximum excitation of 343 nm and a maximum emission of 737 nm.

StarBright dyes are powered by Bio-Rad®. STARBRIGHT is a trademark of Bio-Rad Laboratories, Inc.
Excitation Laser: Ultraviolet Laser (355 nm)
Application Notes: Clone HK1.4 does not block the binding of clone RB6-8C5⁸.

Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells^1-3 and immunohistochemistry of frozen sections⁴.

Antigen Details

Structure: 14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution: Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8⁺ T cells.
Cell Type: Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.
Gene ID: 17067 View all products for this Gene ID
UniProt: View information about Ly-6C on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

Related Pages & Pathways

Related FAQs

What is a “StarBright panel”?: A StarBright panel is a multiplex panel that includes at least one StarBright conjugate.
Will my StarBright panel be provided as a cocktail?: StarBright products purchased as part of a panel or StarBright Basic Panel will be provided in individual vials. To order premade cocktails, please contact our Custom Solutions Team.
Are StarBright GMP/ASR products available?: StarBright ASR and GMP products are coming soon.
Are StarBright custom products available?: Yes, please contact our Custom Solutions Team.
Will StarBright isotype controls be offered?: We are not planning to offer StarBright isotype controls at this time. Fluorescence Minus One (FMO) controls should be used to define gating boundaries.
What amount of a StarBright conjugate should I use?: Antibody conjugates should be titrated for optimal performance in each application. To directly compare performance with a traditional or polymer-conjugated antibody, we recommend using the same volume of each product for analysis. Due to differences in the quantification of product mass, StarBright Dyes are prepared in equivalent volumes.
Why shouldn’t I compare the mass (μg) of a StarBright conjugate to a traditional or polymer conjugate?: For StarBright conjugates, the reported mass refers to the mass of the dye component. For traditional conjugates, the reported mass refers to the mass of the antibody component. This is because traditional conjugates have multiple dye molecules attached to one antibody, while StarBright conjugates have multiple antibodies attached to a single StarBright Dye.

Other Formats

View All Ly-6C Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-mouse Ly-6C (Maxpar® Ready)	HK1.4	FC,CyTOF®
Brilliant Violet 785™ anti-mouse Ly-6C	HK1.4	FC
PE/Dazzle™ 594 anti-mouse Ly-6C	HK1.4	FC
APC/Fire™ 750 anti-mouse Ly-6C	HK1.4	FC
TotalSeq™-A0013 anti-mouse Ly-6C	HK1.4	PG
Brilliant Violet 650™ anti-mouse Ly-6C	HK1.4	FC
TotalSeq™-C0013 anti-mouse Ly-6C	HK1.4	PG
TotalSeq™-B0013 anti-mouse Ly-6C	HK1.4	PG
APC anti-mouse Ly-6C	HK1.4	FC
Pacific Blue™ anti-mouse Ly-6C	HK1.4	FC
Purified anti-mouse Ly-6C	HK1.4	FC,IHC-F
Biotin anti-mouse Ly-6C	HK1.4	FC
FITC anti-mouse Ly-6C	HK1.4	FC
Alexa Fluor® 647 anti-mouse Ly-6C	HK1.4	FC
PE anti-mouse Ly-6C	HK1.4	FC
PerCP/Cyanine5.5 anti-mouse Ly-6C	HK1.4	FC
PE/Cyanine7 anti-mouse Ly-6C	HK1.4	FC
Alexa Fluor® 488 anti-mouse Ly-6C	HK1.4	FC
PerCP anti-mouse Ly-6C	HK1.4	FC
Alexa Fluor® 700 anti-mouse Ly-6C	HK1.4	FC
APC/Cyanine7 anti-mouse Ly-6C	HK1.4	FC
Brilliant Violet 570™ anti-mouse Ly-6C	HK1.4	FC
Brilliant Violet 421™ anti-mouse Ly-6C	HK1.4	FC
Brilliant Violet 510™ anti-mouse Ly-6C	HK1.4	FC
Brilliant Violet 605™ anti-mouse Ly-6C	HK1.4	FC
Brilliant Violet 711™ anti-mouse Ly-6C	HK1.4	FC
APC/Fire™ 810 anti-mouse Ly-6C Antibody	HK1.4	FC
Spark Red™ 718 anti-mouse Ly-6C	HK1.4	FC
PE/Fire™ 810 anti-mouse Ly-6C	HK1.4	FC
Spark UV™ 387 anti-mouse Ly-6C	HK1.4	FC
Spark Blue™ 550 anti-mouse Ly-6C (Flexi-Fluor™)	HK1.4	FC
Spark Blue™ 574 anti-mouse Ly-6C (Flexi-Fluor™)	HK1.4	FC
PE/Cyanine5 anti-mouse Ly-6C	HK1.4	FC
Spark PLUS B550™ anti-mouse Ly-6C	HK1.4	FC
PerCP/Fire™ 806 anti-mouse Ly-6c Antibody	HK1.4	FC
Spark YG™ 581 anti-mouse Ly-6C (Flexi-Fluor™)	HK1.4	FC
Spark YG™ 593 anti-mouse Ly-6C (Flexi-Fluor™) Antibody	HK1.4	FC
Spark NIR™ 685 anti-mouse Ly-6C (Flexi-Fluor™) Antibody	HK1.4	FC
StarBright UltraViolet 740 anti-mouse Ly-6C	HK1.4	FC
StarBright UltraViolet 795 anti-mouse Ly-6C	HK1.4	FC
Spark PLUS UV395™ anti-mouse Ly-6C	HK1.4	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 07/23/2025

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.