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Cell-Vive™ GMP Ultra-LEAF™ Biotin anti-HU CD25 RecAb SF

Pricing & Availability
Clone
S20019D.Rec (See other available formats)
Other Names
Low affinity IL-2R, IL-2R α chain, Tac, p55
Isotype
Mouse IgG1, κ
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Product Citations
publications
S20019DRec_CV_GMP_ULEAF_BIOTIN_CD25_RecAb_SF_082925
Human peripheral lymphocytes were stimulated for 3 days with Cell Activation Cocktail (without Brefeldin A) (cat#: 423301). Cells were washed and stained with Cell-ViveTM GMP Ultra-LEAFTM Biotin anti-human CD25 RecAb SF at 0.25 µg per test (Clone S20019D.Rec, cat#: 385227, filled histogram) or an isotype control (Clone MOPC-21, cat#: 400103, open histogram). Both primary stains were followed by PE Streptavidin (cat#: 405203).
  • S20019DRec_CV_GMP_ULEAF_BIOTIN_CD25_RecAb_SF_082925
    Human peripheral lymphocytes were stimulated for 3 days with Cell Activation Cocktail (without Brefeldin A) (cat#: 423301). Cells were washed and stained with Cell-ViveTM GMP Ultra-LEAFTM Biotin anti-human CD25 RecAb SF at 0.25 µg per test (Clone S20019D.Rec, cat#: 385227, filled histogram) or an isotype control (Clone MOPC-21, cat#: 400103, open histogram). Both primary stains were followed by PE Streptavidin (cat#: 405203).
Cat # Size Price Quantity Check Availability Save
385227 100 µg $492
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385228 1 mg $2525
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Description

CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.

Product Details
Quality Statement

BioLegend Cell-Vive™ GMP Ultra-LEAF™ antibodies are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12 in a dedicated GMP facility compliant with ISO 13485:2016. Specifications and processes include

  • Ultra-Low endotoxin level (< 0.01 EU/µg)
  • Manufactured under serum-free conditions
  • Bulks tested negative for mycoplasma and bacterial/fungal growth
  • Serum-free hybridomas are negative for the presence of murine virus
  • Batch-to-batch consistency
  • Vendor qualification
  • Raw material traceability and documentation
  • Documented procedures and employee training
  • Equipment maintenance and monitoring records
  • Lot-specific certificates of analysis
  • QA review of released products
  • Quality audits per ISO 13485:2016
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Formulation
0.1 µm filtered in phosphate-buffered solution, pH 7.2, with a final concentration of HPCD at 5%. Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Endotoxin Level
Less than 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
Preparation
GMP Ultra-LEAF™ (Low Endotoxin, Azide-Free) Recombinant Serum Free antibody purified by affinity chromatography
Concentration
1 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Disclaimer

BioLegend Cell-Vive™ GMP Ultra-LEAF™ antibodies are for research use only. Suitable for ex vivo cell processing use. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

Antigen Details

Structure
Type I transmembrane glycoprotein, 55 kD
Distribution

Activated T cells and B cells, monocytes/macrophages, Treg

Function
Associates with IL-2 receptor β (CD122) and γ chains (CD132) to form high affinity IL-2R complex
Ligand/Receptor
IL-2
Antigen References
  1. Taniguchi T, et al. 1993. Cell. 73:5.
  2. Waldmann T. 1991. J. Biol. Chem. 266:2681.
Gene ID
3559 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 2    Revision Date: 10/15/2025

For Research Use Only. Suitable for ex vivo cell processing use. Not for injection or diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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