Loading...

RBC Lysis Buffer (10X)

Pricing & Availability
Regulatory Status
RUO
Other Names
Red blood cell lysis buffer
Ave. Rating
Submit a Review
Product Citations
publications
Cat # Size Price Quantity Check Availability Save
420301 100 mL $65
Check Availability


Need larger quantities of this item?
Request Bulk Quote
420302 500 mL $206
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Red Blood Cell (RBC) Lysis Buffer has been designed, formulated, and tested to ensure optimal lysis of RBCs in single cell suspensions with minimal effects on leukocytes. RBC Lysis Buffer is supplied as a 10X solution containing ammonium chloride, potassium carbonate, and EDTA, and should be diluted in deionized water prior to use. Nucleated RBCs are not effectively lysed with ammonium chloride. Test

Product Details
Technical data sheet

Product Details

Formulation
This red blood cell (RBC) lysis buffer is supplied as a 10X solution and should be diluted to 1X in deionized water. 100 ml of 10X concentrate will yield a quantity of 1X solution that is sufficient to lyse 500 samples.
Preparation
The pH of the 1X solution should fall within the range of pH 7.1-7.4. Adjust the pH if necessary. Warm the 1X solution to room temperature prior to use.
Storage & Handling
Store RBC lysis buffer between 2°C and 8°C.
Application Notes

A. Lysis of Mouse Spleen RBCs:
1. Harvest mouse spleen and prepare a single cell suspension.
2. Pellet the cells by centrifugation (350 x g); aspirate the supernatant.
3. Dilute the 10X Red Blood Cell Lysis Buffer to 1X working concentration with deionized water and resuspend the pellet in 5 ml of 1X Lysis Buffer.
4. Incubate on ice for 4-5 minutes with occasional shaking.
5. Stop the reaction by diluting the Lysis Buffer with 20-30 ml of 1X PBS.
6. Spin the cells (350 x g) and discard the supernatant.
7. Resuspend the pellet in the appropriate buffer (e.g., BioLegend Cell Staining Buffer Cat. No. 420201), wash 1X.
8. Count cells, adjust density, and proceed with cell staining procedures.
B. Lysis of Human Peripheral Blood RBCs:
1. Dilute the 10X RBC Lysis Buffer to 1X working concentration with deionized water. Warm the 1X solution to room temperature prior to use.
2. Add 2.0 ml of 1X RBC Lysis Buffer to each tube containing up to 100 µl of whole blood.
3. Gently vortex each tube immediately after adding the lysing solution. Incubate at room temperature, protected from light, for 10-15 minutes.
4. Centrifuge 350 x g for 5 minutes. Aspirate supernatant without disturbing pellet.
5. Resuspend the pellet in the appropriate buffer (e.g., BioLegend Cell Staining Buffer, Cat. No. 420201), wash 1X.
6. Resuspend and proceed with further procedures.

Application References

(PubMed link indicates BioLegend citation)
  1. Adams RA, et al. 2007. J. Exp. Med. 204:571.
  2. Feild-Corbett C, et al. 2009. Stem Cells. PubMed
  3. de Resende MM, et al. 2010. Physiol Genomics. 42:437. PubMed
  4. Roybal JD, et al. 2011. Mol Cancer Res. 9:25. PubMed
  5. Boldin MP, et al. 2011. J Exp Med. PubMed
  6. Kahlenberg JM, et al. 2011. J. Immunol. 187:6143. PubMed
  7. Deiuliis JA, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 302:399. PubMed
  8. Wu CY, et al. 2012. PLoS One. 7:e42365. PubMed
  9. Hsia BJ, et al. 2012. J Allergy Clin Immunol. 130:205. PubMed
  10. Rico C, et al. 2012. Carcinogenesis. 33:2283. PubMed
  11. Jin J, et al. 2012. Arterioscler Thromb Vasc Biol. 32:2901. PubMed
  12. Saraiva AM, 2013. Hum Immunol. 74:207. PubMed
  13. Koon HW, et al. 2013. J Vis exp. 68:4208. PubMed
  14. Ma Dy, et al. 2013. J Virol. 87:3361. PubMed
  15. Han W, 2013. J. Immunol. 190:4786. PubMed
  16. Garcia-Acros I, et al. 2013. J Biol Chem. 288:14046. PubMed
  17. Zhao JL, et al. 2013. Elife. 21:537. PubMed
  18. Guo W, et al. 2013. J Gerontol A Biol Sci Med Sci. PubMed
  19. Yamasaki S, et al. 2013. Clin Vaccine immunol. 20:1508. PubMed
  20. Kraft P, et al. 2013. Stroke. 44:3202. PubMed
  21. Liu X, et al. 2013. Invest Ophthalmol Vis Sci. 54:7386. PubMed
  22. Fullerton AM, et al. 2013. Toxicol Sci. 136:72. PubMed
  23. Cekic C, et al. 2013. J Exp Med. 210:2693. PubMed
  24. Varga T, et al. 2013. J. Immunol. 191:5695. PubMed
  25. Ma Y, et al. 2014. Cancer Res. 74:436. PubMed
  26. Chang YJ, et al. 2014. Cytotherapy. 16:560. PubMed
  27. Kim MJ, et al. 2014. Mol Pharmacol. 86:284. PubMed
  28. Cekic C, et al. 2014. Cancer Res. 74:7250. PubMed
  29. Szulzewsky F, et al. 2015. PloS One. 10:116644. PubMed
Product Citations
  1. Sandovici I, et al. 2022. Dev Cell. 57:63. PubMed
  2. Zhou B, et al. 2020. Nat Immunol. 1.868055556. PubMed
  3. Tan J, et al. 2021. iScience. 24(8):102835. PubMed
  4. Asem M, et al. 2020. Cancer Res. 1156:80. PubMed
  5. Lin C, et al. 2020. Cancer Immunol Res. 632:8. PubMed
  6. Tsai AG, et al. 2020. Nat Med. 408:26. PubMed
  7. Shah D, et al. 2021. Oncoimmunology. 10:1939601. PubMed
  8. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  9. Dobie R, et al. 2020. Cell Reports. 29(7):1832-1847.e8.. PubMed
  10. Strickley JD, et al. 2019. Nature. 575:519. PubMed
  11. Deiuliis J, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 302:L399. PubMed
  12. Jin J, et al. 2012. Arterioscler Thromb Vasc Biol. 32:2901. . PubMed
  13. Barros-Martins J, et al. 2016. J Immunol. 196: 3642 - 3652. PubMed
  14. Rathakrishnan A, et al. 2020. Viruses. 12: . PubMed
  15. Wang Y, et al. 2021. Cancer Res. 81:384. PubMed
  16. Wang H, et al. 2022. Nat Biomed Eng. 6:882. PubMed
  17. Yeh PY, et al. 2022. Sens Actuators B Chem. 353: . PubMed
  18. Ammothumkandy A, et al. 2022. Nat Neurosci. 25:493. PubMed
  19. Melendez E, et al. 2022. Development. 149: . PubMed
  20. Argueta LB, et al. 2022. iScience. 25:104223. PubMed
  21. Wang S, et al. 2022. Adv Sci (Weinh). 9:e2201258. PubMed
  22. Meena AS, et al. 2022. Cell Rep. 39:110937. PubMed
  23. Zhang Y, et al. 2022. Vaccines (Basel). 10: . PubMed
  24. Sugimoto S, et al. 2022. Nat Metab. 4:775. PubMed
  25. Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed
  26. Isildar B, et al. 2022. Inflamm Regen. 42:55. PubMed
  27. Xu Q, et al. 2022. Front Med (Lausanne). 9:940554. PubMed
  28. Alexandrou AT, et al. 2022. iScience. 25:105546. PubMed
  29. Wang Y, et al. 2022. Front Endocrinol (Lausanne). 13:1036517. PubMed
  30. He Y, et al. 2022. Front Immunol. 13:986202. PubMed
  31. Tratwal J, et al. 2022. Front Endocrinol (Lausanne). 13:1001210. PubMed
  32. Xie Z, et al. 2022. Cell Discov. 8:133. PubMed
  33. Xiang X, et al. 2022. Medicine (Baltimore). 101:e32558. PubMed
  34. Yu Z, et al. 2023. Cancer Res. 83:700. PubMed
  35. Ding D, et al. 2023. J Med Chem. 66:1467. PubMed
  36. Oh S, et al. 2023. Nat Biotechnol. :. PubMed
  37. Marritt KL, et al. 2023. Front Immunol. 13:1087991. PubMed
  38. He T, et al. 2023. Immun Inflamm Dis. 11:e759. PubMed
  39. Vyas M, et al. 2023. Front Immunol. 13:1098445. PubMed
  40. Li XF, et al. 2023. J Exp Med. 220: . PubMed
  41. Wang J, et al. 2023. Inflamm Regen. 43:12. PubMed
  42. Ma Y, et al. 2022. Am J Physiol Heart Circ Physiol. 322:H622. PubMed
  43. Feng J, et al. 2022. Immunity. 55:405. PubMed
  44. Rustagi Y, et al. 2022. Diabetes. 71:1149. PubMed
  45. Guennoun R, et al. 2022. Cancers (Basel). 14:. PubMed
  46. Zhuo Y, et al. 2022. J Immunother Cancer. 10:. PubMed
  47. Knelson EH, et al. 2022. Cancer Immunol Res. 10:947. PubMed
  48. Wakiyama H, et al. 2022. Cancer Immunol Res. 10:1386. PubMed
  49. Roweth HG, et al. 2022. Sci Adv. 8:eabo5224. PubMed
  50. Zhong J, et al. 2022. J Adv Res. 41:145. PubMed
  51. Yun HJ, et al. 2022. Int J Mol Sci. 23:. PubMed
  52. Daum P, et al. 2023. Front Immunol. 13:991347. PubMed
  53. Rao D, et al. 2023. Eur J Immunol. 53:e2250258. PubMed
  54. Le DT, et al. 2023. iScience. 26:106059. PubMed
  55. Romo-Rodríguez R, et al. 2023. Arch Med Res. 54:197. PubMed
  56. Mabire M, et al. 2023. Nat Commun. 14:1830. PubMed
  57. MacPherson KP, et al. 2023. Front Cell Neurosci. 17:895017. PubMed
  58. Chen Z, et al. 2023. Nat Commun. 14:1839. PubMed
  59. Martins Nascentes Melo L, et al. 2023. J Immunother Cancer. 11:. PubMed
  60. Ramalingam P, et al. 2023. Nat Commun. 14:2018. PubMed
  61. Choi YJ, et al. 2023. Nat Commun. 14:2045. PubMed
  62. He Q, et al. 2023. Parasit Vectors. 16:139. PubMed
  63. Pattnaik A, et al. 2023. Vaccines (Basel). 11:. PubMed
  64. Mulcrone PL, et al. 2023. Sci Rep. 13:8436. PubMed
  65. Villalba N, et al. 2023. J Neuroinflammation. 20:127. PubMed
  66. Song S, et al. 2023. Bioact Mater. 28:358. PubMed
  67. Matsudaira T, et al. 2023. Commun Biol. 6:665. PubMed
  68. Pracht K, et al. 2021. Eur J Immunol. 51:1089. PubMed
  69. Radwanska M, et al. 2020. Methods Mol Biol. 2116:721. PubMed
  70. Rendon CJ, et al. 2022. Front Endocrinol (Lausanne). 13:995499. PubMed
  71. Effern M, et al. 2022. STAR Protoc. 3:101038. PubMed
  72. Della Verde G, et al. 2022. Life Sci Alliance. 5:. PubMed
  73. Su C, et al. 2021. Front Oncol. 11:719564. PubMed
  74. Herisson F, et al. 2018. Nat Neurosci. 21:1209. PubMed
  75. Jacoby E, et al. 2021. NPJ Regen Med. 6:58. PubMed
  76. Chen Z, et al. 2020. Glia. 68:2148. PubMed
  77. Seow JJW, et al. 2021. Front Med (Lausanne). 8:603374. PubMed
  78. Shin J, et al. 2020. Hum Gene Ther. . PubMed
  79. Bouchareychas L, et al. 2021. iScience. 24(8):102847. PubMed
  80. Spengler J, et al. 2016. J Infect Dis. 214: S308 - S318. PubMed
  81. Zheng M, et al. 2016. J Immunol. 196: 4122 - 4131. PubMed
  82. St-Pierre S, et al. 2016. PLoS One. 11: 0150230. PubMed
  83. Ensan S, et al. 2015. Nat Immunol. 17: 159-168. PubMed
  84. Jaiswal M, et al. 2015. Sci Rep. 5: 15221. PubMed
  85. Hou J, et al. 2015. J Mol Cell Biol. 7: 338-350. PubMed
  86. Fujii H, et al. 2015. PLoS One. 10: 0133216. PubMed
  87. Ma H, et al. 2021. Front Immunol. 12:760095. PubMed
  88. McGill CJ, et al. 2021. STAR Protoc. 2:100948. PubMed
  89. Lutze J, et al. 2022. Methods Mol Biol. 2394:299. PubMed
  90. Hosseini A, et al. 2022. Evid Based Complement Alternat Med. 2022:5639226. PubMed
  91. Liu W, et al. 2017. Int J Environ Res Public Health. 10.3390/ijerph14060592. PubMed
  92. Pietrocola F, et al. 2017. Autophagy. 0.935416667. PubMed
  93. Garcia-Fabiani MB, et al. 2020. STAR Protoc. :1. PubMed
  94. Zhang J, et al. 2021. Aging (Albany NY). 13:17880. PubMed
  95. Press AT, et al. 2021. EMBO Mol Med. 13:e14436. PubMed
  96. Sima LE, et al. 2021. J Immunother Cancer. 9:. PubMed
  97. Chou YJ, et al. 2020. Sci Rep. 10:8422. PubMed
  98. Wang J, et al. 2020. Sci Rep. 10:12785. PubMed
  99. Ma Y, et al. 2014. Cancer Res. 74:436. PubMed
  100. Magalhães L, et al. 2015. PLoS Negl Trop Dis. 10: 0003816. PubMed
  101. Moraes-Vieira P, et al. 2016. Diabetes. 65: 1317 - 1327. PubMed
  102. Bikorimana JP, et al. 2022. Cell Rep Med. 3:100534. PubMed
  103. Zhang L, et al. 2022. JCI Insight. 7:. PubMed
  104. Baldominos P, et al. 2022. STAR Protoc. 3:101795. PubMed
  105. Wu Y, et al. 2021. Nat Biomed Eng. 5:1336. PubMed
  106. Meng S, et al. 2022. Exp Anim. . PubMed
  107. Kokkinopoulos I, et al. 2021. Sci Rep. 11:15759. PubMed
  108. Clark EL, et al. 2017. PLoS Genet. 13:e1006997. PubMed
  109. Cantor JR, et al. 2017. Cell. 169:258. PubMed
  110. Ji WJ, et al. 2017. Cytometry B Clin Cytom. 92:371. PubMed
  111. Johnson CL, et al. 2021. Am J Physiol Heart Circ Physiol. 321:H756. PubMed
  112. Stringhini M, et al. 2021. MAbs. 13:1868066. PubMed
  113. Devalaraja S, et al. 2020. Cell. 1098:180. PubMed
  114. Elisabeth Schrumpf, Xiaojun Jiang 2017. Physiol Rep. 10.14814/phy2.13117. PubMed
  115. Boldin M, et al. 2011. J Exp Med. 208:1189. PubMed
  116. Sun CC, et al. 2020. Genome Med. 0.553472222. PubMed
  117. Bravo–San Pedro JM, et al. 2019. Cell Metab. 30:754. PubMed
  118. AC Belkina, JE Snyder-Cappione 2017. Cytometry A. 91:175-179. PubMed
  119. Brunmeir R, et al. 2016. PLoS Genet. 12:e1006474. PubMed
  120. Pridans C, et al. 2016. J Immunol. 197: 2297 - 2305. PubMed
  121. Mircheff A, et al. 2016. Ocul Surf. 14:460-483. PubMed
  122. Pennington L, et al. 2016. Nat Commun. 7:11610. PubMed
  123. Koon H, et al. 2012. J Vis Exp. 68: 4208. PubMed
  124. Chen J, et al. 2022. Nat Commun. 13:6759. PubMed
  125. Wang D, et al. 2021. Signal Transduct Target Ther. 6:376. PubMed
  126. Machado H, et al. 2021. PLoS Pathog. 17:e1009933. PubMed
  127. Yan X, et al. 2022. Int J Biol Sci. 18:585. PubMed
  128. Singh SS, et al. 2021. Eur J Med Chem. 216:113312. PubMed
  129. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  130. Zhai Z, et al. 2021. Medicine (Baltimore). 100:e26214. PubMed
  131. Hu D, et al. 2021. J Cell Mol Med. 25:2900. PubMed
  132. Jones N, et al. 2021. Nat Commun. 12:1209. PubMed
  133. Sakamoto S, et al. 2020. OncoImmunology. 8(12):e1671760.. PubMed
  134. Rico C, et al. 2012. Carcinogenesis. 33:2283. PubMed
  135. Hamour S, et al. 2015. PLoS One. 10: 0136238. PubMed
  136. Bazett M, Haston M 2016. Sci Rep. 6:19189. PubMed
  137. Luo B, et al. 2016. J Neurosci. 36: 9590 - 9603. PubMed
  138. Patera A, et al. 2016. J Leukoc Biol. 100(6):1239-1254. PubMed
  139. Pokhrel RH, et al. 2022. Int J Mol Sci. 23:. PubMed
  140. Huang L, et al. 2021. J Cell Physiol. 236:7308. PubMed
  141. Whytock KL, et al. 2022. iScience. 25:104772. PubMed
  142. Neufeld L, et al. 2021. Sci Adv. 7:. PubMed
  143. Friedman G, et al. 2020. Nat Cancer. 1:692. PubMed
  144. Kumar A, et al. 2021. Part Fibre Toxicol. 18:43. PubMed
  145. Tanaka Y, et al. 2021. Int J Mol Sci. 22:. PubMed
  146. Scherer SD, et al. 2021. Breast Cancer Res. 23:100. PubMed
  147. Jiang J, et al. 2018. Cancer Med. 3.504861111. PubMed
  148. Chen Z, et al. 2017. Cancer Res. 77:2266. PubMed
  149. Kim M, et al. 2017. Int J Mol Sci. 10.3390/ijms18061275. PubMed
  150. Jeong J, et al. 2021. Immune Netw. 21:e31. PubMed
  151. Sitaraman S, et al. 2021. JCI Insight. 6:. PubMed
  152. Orr AN, et al. 2021. Int J Mol Sci. 22:. PubMed
  153. Saraiva A, et al. 2013. Hum Immunol. 74:207. PubMed
  154. Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed
  155. Facchini M, et al. 2014. J Vis Exp. 85: 51019. PubMed
  156. Ilievski V, et al. 2015. PLoS One. 10: 0136502. PubMed
  157. Mo L, et al. 2022. Front Oncol. 12:871489. PubMed
  158. Dang Y, et al. 2022. Cancer Cell Int. 22:88. PubMed
  159. Deng J, et al. 2021. Breast Cancer Res Treat. 185:85. PubMed
  160. Vivanco Gonzalez N, et al. 2022. STAR Protoc. 3:101280. PubMed
  161. Wang K, et al. 2017. Molecular Immunology. 10.1016/j.molimm.2017.04.016. PubMed
  162. Appelbe O, et al. 2017. J Control Release.. 10.1016/j.jconrel.2017.09.043. PubMed
  163. Tang J, et al. 2017. Proc Natl Acad Sci U S A. 114:E7441. PubMed
  164. Liccardi G, et al. 2019. Mol Cell. 73:413. PubMed
  165. Kildisiute G, et al. 2021. Sci Adv. 7: . PubMed
  166. Schiferle EB, et al. 2021. Sci Adv. 7: . PubMed
  167. Devalaraja S, et al. 2020. STAR Protoc. 1:100188. PubMed
  168. Bigenwald C, et al. 2021. Nat Med. 27:851. PubMed
  169. Gingrich AA, et al. 2021. Front Immunol. 12:670309. PubMed
  170. Patil R, et al. 2019. J Biomed Opt. 24:1. PubMed
  171. Ongaro T, et al. 2020. Oncotarget. 3.026388889. PubMed
  172. Kahlenberg J, et al. 2011. J Immunol. 187:6143. PubMed
  173. Han W, et al. 2013. J Immunol. 190:4786. PubMed
  174. Yamasaki S, et al. 2013. Clin Vaccine Immunol. 20:1508. PubMed
  175. Fukui R, et al. 2016. J Immunol. 196: 416 - 427. PubMed
  176. Rodriguez-Garcia M, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.72. PubMed
  177. Qin L, et al. 2022. Nat Commun. 13:6051. PubMed
  178. Escudero-Lara A, et al. 2022. Front Behav Neurosci. 16:946975. PubMed
  179. Zhang L, et al. 2021. Methods Mol Biol. 2388:175. PubMed
  180. Evans AK, et al. 2020. Neurobiol Dis. 146:105089. PubMed
  181. Wang B, et al. 2017. Stem Cell Res Ther.. 10.1186/s13287-017-0744-6. PubMed
  182. Anstine L,et al. 2017. J Am Heart Assoc.. 10.1161/JAHA.117.007097. PubMed
  183. Judge SJ, et al. 2020. J Clin Invest. 130:3051. PubMed
  184. Choi JW, et al. 2020. PLoS One. 15:e0235518. PubMed
  185. Alban TJ, et al. 2020. Front Immunol. 1.285416667. PubMed
  186. Jiang S, et al. 2017. Toxicology Lett. 10.1016/j.toxlet.2017.03.015. PubMed
  187. Martínez-Muriana A, et al. 2020. Stem Cell Res Ther. 11:53. PubMed
  188. Pennig J, et al. 2019. Sci Rep. 9:17937. PubMed
  189. Agrawal V, et al. 2016. J Leukoc Biol. 100: 261 - 274. PubMed
  190. Beaudoin-Bussières G, et al. 2022. Cell Rep. 38:110368. PubMed
  191. Hu H, et al. 2021. Cancer Immunol Res. 9:1298. PubMed
  192. Zhong W, et al. 2022. Nat Commun. 13:4390. PubMed
  193. So EY, et al. 2021. Am J Physiol Cell Physiol. 321:C569. PubMed
  194. Bunting MD, et al. 2022. Sci Adv. 8:eabk3327. PubMed
  195. Elphinstone R, et al. 2017. Infect Immun. 10.1128/IAI.00371-17. PubMed
  196. Kernfeld EM, et al. 2018. Immunity. 48:1258. PubMed
  197. Cheng Q, et al. 2020. Nat Nanotechnol. 0.842361111. PubMed
  198. Xie Z, et al. 2020. Light Sci Appl. 0.486805556. PubMed
  199. Alghamri MS, et al. 2020. Methods Enzymol. 632:215. PubMed
  200. Yu Z, et al. 2021. Front Oncol. 11:659251. PubMed
  201. Deng J, et al. 2021. Nat Cancer. 2:503. PubMed
  202. Nwani NG, et al. 2019. Cancers (Basel). 0.806944444. PubMed
  203. Ramalingam P, et al. 2020. Nat Commun. 11:666. PubMed
  204. Garcia-Arcos I, et al. 2013. J Biol Chem. 288:14046. PubMed
  205. Sci T 2013. Toxicol Sci. 136:72. PubMed
  206. Metz H, et al. 2016. Proc Natl Acad Sci U S A. 113: 8795 - 8800. PubMed
  207. Tavakol DN, et al. 2021. Curr Protoc. 1:e275. PubMed
  208. Ullah I, et al. 2021. Immunity. 54:2143. PubMed
  209. He Y, et al. 2021. Front Immunol. 12:641206. PubMed
  210. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  211. Ercoli G, et al. 2022. Clin Transl Immunology. 11:e1366. PubMed
  212. Meng YM, et al. 2021. Nat Commun. 12:6011. PubMed
  213. Zhu QY, et al. 2021. Nat Commun. 12:6624. PubMed
  214. Patel M, et al. 2021. Cancer Res. 81:3985. PubMed
  215. Shi X, et al. 2021. Nat Commun. 12:2547. PubMed
  216. Ramaswamy G, et al. 2017. Sci Rep. 10.1038/srep45140. PubMed
  217. Mirando AC, et al. 2020. Oncoimmunology. 9:1760685. PubMed
  218. Rosenbluth JM, et al. 2020. Nat Commun. 11:1711. PubMed
  219. Lizotte P, et al. 2016. Sci Rep. 6:31745. PubMed
  220. Hills LB, et al. 2021. J Immunol. 206:89. PubMed
  221. Page N, et al. 2021. Nat Commun. 12:1009. PubMed
  222. Pace J, et al. 2022. J Biomed Opt. 27:. PubMed
  223. Pedicona F, et al. 2022. Sci Signal. 15:eabl7989. PubMed
  224. Wen J, et al. 2017. J Hematol Oncol. 10.1186/s13045-017-0489-9. PubMed
  225. Ahmad S,et al. 2017. Prog Neuropsychopharmacol Biol Psychiatry. . 10.1016/j.pnpbp.2017.10.001. PubMed
  226. Sharma A, et al. 2020. Cell. 183(2):377-394.e21. PubMed
  227. Hang J, et al. 2019. Cancer Med. 1.254166667. PubMed
  228. Tan X, et al. 2019. Sci Rep. 2.7125. PubMed
  229. Lienemann PS, et al. 2020. Adv Sci (Weinh). 7:1903395. PubMed
  230. Zhao J, et al. 2013. Elife. 21:537. PubMed
  231. Weber G, et al. 2015. Surgery. 158: 248-254. PubMed
  232. Jiang H, et al. 2016. Nat Med. 10.1038/nm.4123. PubMed
  233. Elgueta R, et al. 2016. J Immunol. 197: 3970 - 3981. PubMed
  234. Fidalgo MF, et al. 2022. Life Sci Alliance. 5:. PubMed
  235. Stirling ER, et al. 2022. J Immunother Cancer. 10:. PubMed
  236. Zhai K, et al. 2021. Nat Cancer. 2:1136. PubMed
  237. Yang A, et al. 2018. Cancer Discov. 0.525. PubMed
  238. Rentsendorj A, et al. 2017. Brain Behav Immun. 10.1016/j.bbi.2017.08.019. PubMed
  239. Zhao D, et al. 2020. Cancer Discov. 10:1374. PubMed
  240. Castoldi F, et al. 2020. Cell Death Discov. 0.339583333. PubMed
  241. Wang L, et al. 2020. EMBO J. 39:e104514. PubMed
  242. Morris C, et al. 2017. MBio. 10.1128/mBio.00036-17. PubMed
  243. Schweppe RE, et al. 2019. Mol Cancer Res. 17:1036. PubMed
  244. Yamada KJ, et al. 2020. PLoS Pathog. 16:e1008354. PubMed
  245. Theurl I, et al. 2016. Nat Med. 10.1038/nm.4146. PubMed
  246. Yan Q, et al. 2022. Clin Transl Med. 12:e1084. PubMed
  247. Hollerbach A, et al. 2021. J Thromb Haemost. . PubMed
  248. Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed
  249. Yang Z, et al. 2021. Nat Commun. 12:4852. PubMed
  250. Zhang Y, et al. 2022. Vaccines (Basel). 10: . PubMed
  251. Majumdar D, et al. 2021. Curr Protoc. 1:e216. PubMed
  252. Dyck S, et al. 2018. J Neuroinflammation. 0.6875. PubMed
  253. Nowyhed H, et al. 2017. Sci Rep. 7:40273. PubMed
  254. De Domenico E, et al. 2020. STAR Protoc. 1:100233. PubMed
  255. Wang Z, et al. 2020. Cell Rep. 33:108472. PubMed
  256. Sadik A, et al. 2020. Cell. 1252:182. PubMed
  257. Hiza H, et al. 2021. Sci Rep. 11:13190. PubMed
  258. Ito T, et al. 2021. Nat Commun. 12:2232. PubMed
  259. Yang YS, et al. 2020. Mol Ther Methods Clin Dev. 17:922. PubMed
  260. Gangwar RS, et al. 2020. Sci Rep. 10:14348. PubMed
  261. Varga T, et al. 2013. J Immunol. 191:5695. PubMed
  262. Samuelson D, et al. 2016. J Immunol. 196: 2655 - 2665. PubMed
  263. Tsao P, et al. 2016. Proc Natl Acad Sci U S A. 113: 8242 - 8247. PubMed
  264. Soncini M, et al. 2016. Proc Natl Acad Sci U S A. 113: E6219 - E6227. PubMed
  265. Yang C, et al. 2016. Sci Rep. 6:34643. PubMed
  266. Michelotti TC, et al. 2022. Front Cell Dev Biol. 10:1025240. PubMed
  267. Ren X, et al. 2022. STAR Protoc. 3:101818. PubMed
  268. Saber M, et al. 2021. J Neurosci Res. 99:1136. PubMed
  269. Xiang Y, et al. 2022. Skelet Muscle. 12:19. PubMed
  270. Yamazaki T, et al. 2021. Oncoimmunology. 10:1962592. PubMed
  271. Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed
  272. Bowers J, et al. 2017. Front Immunol. 10.3389/fimmu.2017.01221. PubMed
  273. Zhang J, et al. 2020. Pain. 161:1381. PubMed
  274. Heim CE, et al. 2020. Nature Microbiology. 5(10):1271-1284. PubMed
  275. Gladow N, et al. 2020. PLoS One. 15:e0227734. PubMed
  276. Ramachandran P, et al. 2019. Nature. 575:512. PubMed
  277. Canali S, et al. 2017. Blood. 129(4):405-414. PubMed
  278. Shen X, et al. 2016. Sci Rep. 6:38778. PubMed
  279. Sauter K, et al. 2016. Am J Physiol Gastrointest Liver Physiol. 311: G533 - G547. PubMed
  280. Song C, et al. 2015. Blood. 126: 1813 - 1822. PubMed
  281. Sasaki Y, et al. 2015. PLoS One. 10: 0131176. PubMed
  282. Feng D, et al. 2022. Nat Commun. 13:5554. PubMed
  283. Xue X, et al. 2021. Cent Eur J Immunol. 46:191. PubMed
  284. Unsinger J, et al. 2021. J Leukoc Biol. 109:697. PubMed
  285. Ansari M, et al. 2017. Cell Signal. 10.1016/j.cellsig.2017.04.014. PubMed
  286. Ahmad S, et al. 2017. Prog Neuropsychopharmacol Biol Psychiatry. 10.1016/j.pnpbp.2017.06.034. PubMed
  287. Bichsel C, et al. 2017. Sci Rep. 10.1038/s41598-017-09928-1. PubMed
  288. James M, et al. 2017. J Nucl Med. . 10.2967/jnumed.117.189597. PubMed
  289. Annibaldi A, et al. 2018. Mol Cell. 69:566. PubMed
  290. Hill SJ, et al. 2018. Cancer Discov. 1.308333333. PubMed
  291. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  292. Tulasi DY, et al. 2021. Cell Mol Gastroenterol Hepatol. 11:1437. PubMed
  293. Hoffmann J, et al. 2021. Nat Commun. 12:3964. PubMed
  294. Ma D, et al. 2013. J Virol. 87:3361. PubMed
  295. Kraft P, et al. 2013. Stroke. 44:3202. PubMed
  296. Kim M, et al. 2014. Mol Pharmacol. 86:284. PubMed
  297. Cekic C, et al. 2014. Cancer Res. 74:7250. PubMed
  298. Pankova D, et al. 2016. Mol Cancer Res. 14: 287 - 295. PubMed
  299. Yang YS, et al. 2022. Nat Commun. 13:6175. PubMed
  300. Soileau AM, et al. 2022. Vet Sci. 9:. PubMed
  301. McClellan BL, et al. 2022. STAR Protoc. 3:101357. PubMed
  302. Governa V, et al. 2022. Proc Natl Acad Sci U S A. 119. PubMed
  303. Deng Y, et al. 2021. Nat Commun. 12:7041. PubMed
  304. Zhang Z, et al. 2017. Journal of Translational Medicine. 10.1186/s12967-017-1196-6. PubMed
  305. Mai CL, et al. 2021. Theranostics. 11:1059. PubMed
  306. Chen P, et al. 2021. Clin Transl Med. 11:e462. PubMed
  307. Peng X, et al. 2021. Stem Cell Res Ther. 198:12. PubMed
  308. Linder A, et al. 2020. EMBO J. 39:e105071. PubMed
  309. Hsia B, et al. 2012. J Allergy Clin Immunol. 130:205. PubMed
  310. Resende M, et al. 2010. Physiol Genomics. 42:437. PubMed
  311. McLaughlin S, et al. 2019. Nat Commun. 3.795833333. PubMed
  312. Koury J, et al. 2019. PLoS One. 14:e0218670. PubMed
  313. Muñoz-Ruiz M, et al. 2016. Nat Immunol. 10.1038/ni.3424. PubMed
  314. Chang Y, et al. 2014. Cytotherapy. 16:560. PubMed
  315. Li N, et al. 2022. Nat Commun. 13:7281. PubMed
  316. Cordo' V, et al. 2022. Nat Commun. 13:1048. PubMed
  317. Hallisey M, et al. 2022. STAR Protoc. 3:101163. PubMed
  318. Denoth-Lippuner A, et al. 2021. Cell Stem Cell. 28:2020. PubMed
  319. Maas RR, et al. 2021. Nat Protoc. 16:4692. PubMed
  320. Nagree MS, et al. 2022. EMBO Mol Med. 14:e14297. PubMed
  321. Mochizuki M,et al. 2017. J Am Heart Assoc. . 10.1161/JAHA.117.005920. PubMed
  322. Roh–Johnson M, et al. 2017. Dev Cell. 43:549. PubMed
  323. Müller C, et al. 2018. Elife. 7:e34985. PubMed
  324. Ubellacker J, et al. 2017. Breast Cancer Res. 10.1186/s13058-017-0815-8. PubMed
  325. DU T, et al. 2020. Front Immunol. 1.571527778. PubMed
  326. Trial J, et al. 2020. Geroscience. . PubMed
  327. Guo W, et al. 2013. J Gerontol A Biol Sci Med Sci. . PubMed
  328. Liu Y, et al. 2015. Sci Rep. 5: 15134. PubMed
  329. Hodson D, et al. 2016. Proc Natl Acad Sci U S A. 113: 2039-2046. PubMed
  330. Munson D, et al. 2016. Proc Natl Acad Sci U S A. 113: 8272 - 8277. PubMed
  331. Sobecki M, et al. 2022. Cell Stem Cell. 29:1459. PubMed
  332. Li Q, et al. 2022. Signal Transduct Target Ther. 7:222. PubMed
  333. Fielding C, et al. 2022. Nat Commun. 13:543. PubMed
  334. Weaver J, et al. 2017. Part Fibre Toxicol. 10.1186/s12989-017-0206-4. PubMed
  335. Samuelson D, et al. 2017. PLoS Pathogens. 13(6):e1006426. PubMed
  336. Wong C, et al. 2017. J Immunol. 10.4049/jimmunol.1700397. PubMed
  337. Vechetti IJ, et al. 2021. FASEB J. 35:e21644. PubMed
  338. Saber M, et al. 2020. Eur J Neurosci. 52:2791. PubMed
  339. Wu R, et al. 2020. J Immunother Cancer. 8:00. PubMed
  340. Wu C, et al. 2012. PLoS One. 7:e42363. PubMed
  341. Denning W, et al. 2011. PLoS One. 6:e16897. PubMed
  342. Yu H, et al. 2016. Sci Rep. 6:35190. PubMed
  343. Now H, Yoo J 2016. Cell Mol Immunol. 10.1038/cmi.2016.32. PubMed
  344. Bosma M, et al. 2016. Nat Commun. 7: 11314. PubMed
  345. YC S, SJ L 2015. Sci Rep. 5: 12578. PubMed
  346. Szulzewsky F, et al. 2015. PLoS One. 10:116644. PubMed
  347. Visweshwaran SP, et al. 2022. Front Cell Dev Biol. 10:1008898. PubMed
  348. Yeini E, et al. 2021. Nat Commun. 12:1912. PubMed
  349. Wang X, et al. 2021. Sci Transl Med. 13:. PubMed
  350. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  351. Kucykowicz S, et al. 2022. STAR Protoc. 3:101356. PubMed
  352. Kim TW, et al. 2022. Mol Ther Oncolytics. 26:1. PubMed
  353. Kathania M, et al. 2022. Cell Rep. 40:111345. PubMed
  354. Barcia Durán JG, et al. 2021. Commun Biol. 4:406. PubMed
  355. Park HJ, et al. 2021. Int J Mol Sci. 22:. PubMed
  356. Cebrian-Silla A, et al. 2021. Elife. 10:. PubMed
  357. Gupta R, et al. 2020. Cell Stem Cell. 26:527. PubMed
  358. Roybal J, et al. 2011. Mol Cancer Res. 9:25. PubMed
  359. Cekic C, et al. 2013. J Exp Med. 210:2693. PubMed
  360. Shaashua L, et al. 2022. Nat Commun. 13:6513. PubMed
  361. Roy IM, et al. 2021. Bio Protoc. 11:e4130. PubMed
  362. Alam A, et al. 2022. STAR Protoc. 3:101563. PubMed
  363. Xiang Y, et al. 2022. Exp Mol Med. 54:1011. PubMed
  364. Guo Z, et al. 2022. Neurobiol Pain. 12:100096. PubMed
  365. Guo P, et al. 2022. Nat Cell Biol. 24:99. PubMed
  366. Wahane S, et al. 2021. Sci Adv. 7:. PubMed
  367. Alizadeh A, et al. 2018. J Neuroinflammation. 15:53. PubMed
  368. Guo P, et al. 2017. J Clin Invest. 127:4242. PubMed
  369. Tsai SC, et al. 2017. Biomicrofluidics. 11:034122. PubMed
  370. Xie Z, et al. 2020. Light Sci Appl. 9:161. PubMed
  371. Huang N, et al. 2021. Nat Med. 27:892. PubMed
  372. Song X, et al. 2021. FEBS Open Bio. 11:2453. PubMed
  373. Chen S, et al. 2020. Cancer Immunol Res. 1064:8. PubMed
  374. Erady C, et al. 2021. NPJ Genom Med. 6:04. PubMed
  375. de Silva S, et al. 2020. Cancer Immunol Res. 0.493055556. PubMed
  376. Wan C, et al. 2021. Cancer Res. 81:158. PubMed
  377. Field-Corbett C, et al. 2009. Stem Cells. 27:662. PubMed
  378. Zeng Y, et al. 2019. FASEB J. 33:6596. PubMed
  379. Zheng W, et al. 2019. Cell Rep. 29:1747. PubMed
  380. Tomas C, et al. 2019. Sci Rep. 9:11464. PubMed
  381. Kim JK, et al. 2019. Bioengineering (Basel). 6:24. PubMed
  382. Wang X, et al. 2016. J Virol. 90: 10247 - 10258. PubMed
  383. Mohammed R, et al. 2016. Cell Rep. 14:760-771. PubMed
  384. Durward-Diioia M, et al. 2015. Infect Immun . 83: 4759 - 4771. PubMed
  385. Birnie K, et al. 2015. Mol Cancer Res. 13: 1106 - 1118. PubMed
  386. Liu X, et al. 2013. Invest Ophthalmol Vis Sci . 54:7386. PubMed

Antigen Details

Cell Type
Tregs
Gene ID
NA

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 06/10/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

ProductsHere
Login/Register
Remember me
Forgot your password? Reset Password
Request an Account