DAB Substrate Buffer with Stabilizer (Previously Covance catalog# SIG-31049)

Protocol:
1. Add the DAB chromogen to the DAB substrate buffer as described in the table below

Buffer     DAB Chromogen*
5 mL   +   0.2 mL
25 mL   +   1.0 mL
50 mL   +   2.0 mL
*Cat Nos: 926501 & 926503.

2. Apply mixture to tissue section 
3. Incubate the slides for 5-10 min. at room temperature
4. Rinse tissue sections with deionized water
5. Counterstain and mount
6. DAB produces a brown precipitate with almost no background

BioLegend’s DAB Substrate Buffer with Stabilizer consists of stable buffer. The DAB Substrate Buffer with Stabilizer in combination with DAB chromogen reacts with peroxidase to produce brown staining.