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- Other Names
- Thymic stromal lymphopoietin (TSLP)
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- Product Citations
- publications
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Baf3-hIL7a/hTSLPR cell proliferation induced by human TSLP.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 582414 | 50 µg | 1084€ | ||||
| 582416 | 500 µg | 3252€ | ||||
TSLP was initially identified in conditioned medium from a mouse thymic stromal cell line. Human and mouse TSLP are only 43% identical at the amino acid level, and TSLP, like IL-7, exhibits species-specific functions and may function differently in humans and mice. TSLP binds to a heterodimeric receptor constituted by IL-7Ra and TSLPR. TSLPR has low affinity for TSLP, but in combination with IL-7Ra generates a high affinity binding site. TSLP has been associated with the development of allergic disorders in humans, such as asthma, atopic dermatitis, and food allergies. Susceptibility to allergic diseases has been associated with basophil induction by TSLP, and Th2 cytokine production induced by TSLP in mast cells. In addition, TSLP has been associated with the development of Tregs, and blocking TSLPR leads to delayed functional maturation of thymic Tregs. TSLPR has been detected in Tregs from allergic asthmatic patients. TSLP directly activates STAT5 in Tregs and suppresses its suppressive activities; additionally, it inhibits IL-10 production. Human TSLP induces the release of T cell-attracting chemokines (CCL17 and CCL22) from monocytes and enhances the T cell stimulatory capacity of the CD11c+ subset of dendritic cells. TSLP induces proliferation of naïve T cells. In addition, it can drive Th2 differentiation via the induction of IL-4 production and drives Th2 cytokine-mediated inflammation by acting on bone marrow-resident progenitors, which promotes basophil hematopoiesis. TSLP is upregulated in epithelial cells by IL-1α, TNFα, and Th2 cytokines.
Product DetailsBioLegend Cell-Vive™ GMP Recombinant proteins are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12 in a dedicated GMP facility compliant with ISO 13485:2016. Specifications and processes include:
- Low endotoxin level (≤ 0.1 EU/µg)
- Purity (≥ 95% or higher)
- Bioburden testing
- Mycoplasma testing
- Batch-to-batch consistency
- Vendor qualification
- Raw material traceability and documentation
- Documented procedures and employee training
- Equipment maintenance and monitoring records
- Lot-specific certificates of analysis
- Quality audits per ISO 13485:2016
- QA review of released products
Product Details
- Source
- Human TSLP, amino acids Met-Tyr29-Gln159 (Accession# AF338732), was expressed in E. coli.
- Molecular Mass
- The 132 amino acid recombinant protein has a predicted molecular mass of approximately 15 kD. The DTT-reduced protein migrates at approximately 17 kD and non-reduced protein at approximately 18 kD by SDS-PAGE. The N-terminal amino acid is Met.
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N-terminal
Sequence Analysis - Met-Tyr-Asp-Phe-Thr-Asn-(Cys)-Asp-Phe-Glu
- Purity
- ≥ 95%, as determined by Coomassie stained SDS-PAGE
- Formulation
- Protein was lyophilized from 0.1 μm filtered solution containing 20 mM sodium phosphate, 150 mM NaCl, pH 7.2
- Endotoxin Level
- Less than or equal to 0.1 EU per μg of protein as determined by LAL method
- Residual Host Cell Protein Content
- ≤ 0.500 ng/μg by ELISA
- Concentration
- 50 µg and 500 µg sizes are lyophilized
- Storage & Handling
- Unopened lyophilized vials can be stored at ≤ -20°C for 5 years and between 2°C – 8°C for 2 weeks. Reconstituted aliquots can be stored between -20°C to -70°C for 3 months and between 2°C – 8°C for 2 weeks. Reconstitute lyophilized protein in sterile water. Before reconstitution, make sure sterile water and product are at room temperature. Quickly spin the vial or gently tap down on the vial to make sure the lyophilized product is at the bottom of the vial before opening. Use aseptic techniques to add the required volume of reconstitution buffer to the vial, to obtain the recommended stock concentration of 250 μg/mL. Close the vial and leave at ambient temperature for 15-20 minutes. Then gently invert the vial several times or until all the lyophilized product dissolves. Leave the vial at room temperature for another 15 minutes. If small particulates are still observed after 15 minutes, incubate at room temperature for an additional 30 minutes and leave the vial at 2°C - 8°C overnight. Next day, invert the vial several times or gently pipette the solution up and down before use. If needed, transfer the reconstituted stock solution to a sterile container for additional dilution to no less than 100 μg/mL. Small working aliquots in polypropylene tubes can be made after reconstitution and store the vials at -20°C or lower. Avoid freeze/thaw cycles. Carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution.
- Activity
- Cell-Vive™ GMP Recombinant Human TSLP induces proliferation of mouse BaF3-human IL-7a and human TSLPR cotransfectant cells in a dose dependent manner. The ED50 for this effect is 0.4 – 2.4 ng/mL.
- Application
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Bioassay
- Application Notes
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Our lyophilized proteins are validated in-house to maintain activity after shipping at ambient temperature and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech-BioLegend@revvity.com.
- Disclaimer
-
BioLegend Cell-Vive™ GMP Recombinant proteins are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.
Antigen Details
- Structure
- Cytokine
- Ligand/Receptor
- Heterodimeric receptor complex composed of the IL-7 receptor α chain (IL-7Rα) and the TSLP receptor (TSLPR or CRLF2)
- Bioactivity
- Induces proliferation of mouse BaF3-human IL-7a and human TSLPR cotransfectant cells in a dose dependent manner
- Cell Sources
- Epithelial cells, mast cells, basophils, epidermal keratinocytes in atopic dermatitis, synovial fibroblast in arthritis rheumatoid, human airway smooth muscle cells
- Cell Targets
- Monocyte, dendritic cells, activated human basophils, activated human T cells, Langerhans cells, B cells, mast cells, activated pulmonary Tregs, NKT cells
- Antigen References
-
- Levin SD, et al. 1999. J. Immunol. 162:677.
- Reche PA, et al. 2001. J. Immunol. 167:336.
- Omori M and Steven Ziegler. 2007. J. Immunol. 178:1396.
- Rochman I, et al. 2007. J. Immunol. 178:6720.
- Mazzuchelli R, et al. 2008. Blood. 112:3283.
- Siracusa MC, et al. 2011. Nature. 477:229.
- Nguyen KD, et al. 2010. Allergy Asthma Clin. Immunol. 6:4.
- Regulation
- Induces proliferation of naïve T cells, the release of T cell-attracting chemokines (CCL17 and CCL22) from monocytes, and the T cell stimulatory capacity of CD11c+ subset of dendritic cells; drives Th2 differentiation, drives Th2 cytokine-mediated inflammation to promote basophil hematopoiesis
- Gene ID
- 85480 View all products for this Gene ID
- UniProt
- View information about TSLP on UniProt.org
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