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Lipid Droplet Probe Green

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Regulatory Status
RUO
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A_Lipid_Droplet_Probe_Green_1_013124
Live-cell fluorescence imaging of HepG2 cells treated with Oleic acid and stained with Lipid Droplet Probe Green. Nuclei were stained with Hoechst. Images were captured using a 60X objective. Scale bar: 20 µm
  • A_Lipid_Droplet_Probe_Green_1_013124
    Live-cell fluorescence imaging of HepG2 cells treated with Oleic acid and stained with Lipid Droplet Probe Green. Nuclei were stained with Hoechst. Images were captured using a 60X objective. Scale bar: 20 µm
  • B_Lipid_Droplet_Probe_Green_FC_013124
    THP-1 cells treated with Oleic acid stained with Lipid Droplet Probe Green (filled histogram) or left unstained (open histogram).
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421920 125 µL 440€
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Description

Intracellular lipid droplets are cytoplasmic organelles involved in the storage and regulation of triglycerides and cholesterol esters. Lipid Droplet Probe Green is a fluorogenic neutral lipid stain that rapidly accumulates in lipid droplets, where they become brightly fluorescent. The dyes can be used to stain lipid droplets in both live and fixed cells, with no wash step required. Cells can also be fixed and permeabilized after staining. Lipid Droplet Probe Green has an excitation at around 430 nm, and can be excited equally well at 405 nm or 488 nm. In cells, it stains lipid droplets with bright green fluorescence detectable in the FITC channel.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Mouse, Rat
Formulation
1 vial of 125 µL Lipid Droplet Probe Green in DMSO
Storage & Handling
2-8°C
Application

Live cell imaging - Quality tested
FC - Verified
Application Notes

Lipid Droplet Probe Green is a fluorescent dye that specifically stains lipid droplets in live or fixed cells. It does not require a wash step and shows minimal background on cellular membranes or organelles. This probe has an excitation at around 430 nm, and can be excited equally well at 405 nm or 488 nm. In cells, it stains lipid droplets with bright green fluorescence detectable in the FITC channel.

Components:
125 μL of Lipid Droplet Probe Green 1000X Stock Solution in DMSO (Store at 2-8°C, protected from light)

Required Materials Not Included:
Stain Buffer: Phenol-free media, HBSS, or Cell Staining Buffer (Cat. No. 420201)

Imaging Guidelines:
Ex/Em = 427/585 nm
Fluorescence microscope filter set: FITC or AmCyan
Flow cytometry channel: FITC or Pacific Orange

Assay Protocol:

  1. Prepare 1x Lipid Droplet staining solution: Dilute the 1000X Lipid Droplet Probe Stock Solution in culture media (ideally phenol free) at 1:1000 to make 1x staining solution.
    Note: Final volume to be determined by user. We recommend 100 μL per well in a 96-well plate. Staining conditions and probe concentration may be optimized if needed.
  2. Remove media from cells and add the desired volume of 1x staining solution. If cells are in suspension, spin down and resuspend in 1x staining solution.
  3. Optional: Include live-cell nuclear counterstain such as DRAQ5™ (Cat. No. 424101).
  4. Incubate cells at 37°C for 30 minutes (or longer).
  5. Optional: Wash cells twice with buffer, add desired volume of clear buffer or media.
  6. Proceed directly to imaging or flow cytometry analysis or fix cells with 4% PFA or Fixation Buffer (Cat. No. 420801).

Antigen Details

Distribution

Cytoplasm, Lipid Droplets

Biology Area
Cell Biology
Molecular Family
Organelle Markers
Gene ID
NA

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 01.31.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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