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Purified anti-human CA19-9 Antibody

Pricing & Availability
Clone
121SLE (See other available formats)
Regulatory Status
RUO
Other Names
Sialyl Lewis a, 3-Sialyl Lewis, SLea, Sialyl Lewis A, Sodium Salt
Isotype
Mouse IgM, κ
Ave. Rating
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Product Citations
publications
A. 121SLE_PURE_-CA19-9_FC_032425
COLO205 (human colorectal tumor cell line) was stained with purified anti-human CA19-9 (clone 121SLE) (filled histogram) or purified mouse IgM, κ isotype control (open histogram) followed by anti-mouse IgM PE.
  • A. 121SLE_PURE_-CA19-9_FC_032425
    COLO205 (human colorectal tumor cell line) was stained with purified anti-human CA19-9 (clone 121SLE) (filled histogram) or purified mouse IgM, κ isotype control (open histogram) followed by anti-mouse IgM PE.
  • B. 121SLE_PURE_-CA19-9_IHC-P_032425
    Following antigen retrieval using 1X Tris-EDTA Buffer (Cat. No. 422704), formalin-fixed, deparaffinized human pancreas tissue sections were incubated with 5 µg/mL of purified anti-human CD99 (Cat. No. 324502) and purified mouse IgM, κ isotype control (Cat. No. 401602) (panel A) or purified anti-human CA19-9 (clone 121SLE) (panel B) overnight at 4°C. The tissue was then stained with rat anti-mouse IgM Alexa Fluor® 647 (red) (Cat. No. 406526) and rat anti-mouse IgG2a, κ Alexa Fluor® 594 (green) (Cat. No. 407120). Nuclei were counterstained with DAPI (blue) (Cat. No. 422801). Images were captured using a 10X objective. Scale bar: 50 µm
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314209 100 µg 216€
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Description

Carbohydrate Antigen 19-9 (CA19-9), also known as sialyl-Lewis A, is a cell surface glycoprotein complex. CA 19-9 expression requires the Lewis gene product, 1,4-fucosyltransferase, found only in patients with Le (a-b+) or Le (a+b-) blood groups. CA19-9 antigen is highly expressed in a variety of gastrointestinal epithelial malignancies including colon cancer and pancreatic cancer, and in breast cancer and small cell lung cancer, but has a limited expression on normal tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Immunoprecipitate obtained after immunodiffusion of MAb 19-9 and mucins isolated from an ovarian cyst of a 0Le(a+b-) patient
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of  5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. 

Application References
  1. Ragupathi G, et al. 2009. Cancer Immunol Immunother. 58:1397.
  2. Herrero-Zabaleta ME, et al. 1987. Bull Cancer. 74:387.
RRID
AB_3698970 (BioLegend Cat. No. 314209)

Antigen Details

Structure
Branched tetrasaccharide with the sequence Neu5Acα2-3Galβ1-3[Fucα1-4]GlcNAcβ
Function
Elevated levels of CA19-9 can indicate the presence of pancreatic cancer, but it can also be raised in other cancers such as colorectal, stomach, and liver
Ligand/Receptor
sLea is known to be the ligand for endothelial cell selectins
Molecular Family
Adhesion Molecules, Blood Group Markers, Cytokines/Chemokines, Enzymes and Regulators
Gene ID
NA

Related FAQs

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Go To Top Version: 1    Revision Date: 03.24.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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