Loading...

RBC Lysis Buffer (10X)

Pricing & Availability
Regulatory Status
RUO
Other Names
Red blood cell lysis buffer
Ave. Rating
Submit a Review
Product Citations
publications
Cat # Size Price Quantity Check Availability Save
420301 100 mL 52€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
420302 500 mL 165€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Red Blood Cell (RBC) Lysis Buffer has been designed, formulated, and tested to ensure optimal lysis of RBCs in single cell suspensions with minimal effects on leukocytes. RBC Lysis Buffer is supplied as a 10X solution containing ammonium chloride, potassium carbonate, and EDTA, and should be diluted in deionized water prior to use. Nucleated RBCs are not effectively lysed with ammonium chloride. Test

Product Details
Technical Data Sheet (pdf)

Product Details

Formulation
This red blood cell (RBC) lysis buffer is supplied as a 10X solution and should be diluted to 1X in deionized water. 100 ml of 10X concentrate will yield a quantity of 1X solution that is sufficient to lyse 500 samples.
Preparation
The pH of the 1X solution should fall within the range of pH 7.1-7.4. Adjust the pH if necessary. Warm the 1X solution to room temperature prior to use.
Storage & Handling
Store RBC lysis buffer between 2°C and 8°C.
Application Notes

A. Lysis of Mouse Spleen RBCs:
1. Harvest mouse spleen and prepare a single cell suspension.
2. Pellet the cells by centrifugation (350 x g); aspirate the supernatant.
3. Dilute the 10X Red Blood Cell Lysis Buffer to 1X working concentration with deionized water and resuspend the pellet in 5 ml of 1X Lysis Buffer.
4. Incubate on ice for 4-5 minutes with occasional shaking.
5. Stop the reaction by diluting the Lysis Buffer with 20-30 ml of 1X PBS.
6. Spin the cells (350 x g) and discard the supernatant.
7. Resuspend the pellet in the appropriate buffer (e.g., BioLegend Cell Staining Buffer Cat. No. 420201), wash 1X.
8. Count cells, adjust density, and proceed with cell staining procedures.
B. Lysis of Human Peripheral Blood RBCs:
1. Dilute the 10X RBC Lysis Buffer to 1X working concentration with deionized water. Warm the 1X solution to room temperature prior to use.
2. Add 2.0 ml of 1X RBC Lysis Buffer to each tube containing up to 100 µl of whole blood.
3. Gently vortex each tube immediately after adding the lysing solution. Incubate at room temperature, protected from light, for 10-15 minutes.
4. Centrifuge 350 x g for 5 minutes. Aspirate supernatant without disturbing pellet.
5. Resuspend the pellet in the appropriate buffer (e.g., BioLegend Cell Staining Buffer, Cat. No. 420201), wash 1X.
6. Resuspend and proceed with further procedures.

Application References
  1. Adams RA, et al. 2007. J. Exp. Med. 204:571.
  2. Feild-Corbett C, et al. 2009. Stem Cells. PubMed
  3. de Resende MM, et al. 2010. Physiol Genomics. 42:437. PubMed
  4. Roybal JD, et al. 2011. Mol Cancer Res. 9:25. PubMed
  5. Boldin MP, et al. 2011. J Exp Med. PubMed
  6. Kahlenberg JM, et al. 2011. J. Immunol. 187:6143. PubMed
  7. Deiuliis JA, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 302:399. PubMed
  8. Wu CY, et al. 2012. PLoS One. 7:e42365. PubMed
  9. Hsia BJ, et al. 2012. J Allergy Clin Immunol. 130:205. PubMed
  10. Rico C, et al. 2012. Carcinogenesis. 33:2283. PubMed
  11. Jin J, et al. 2012. Arterioscler Thromb Vasc Biol. 32:2901. PubMed
  12. Saraiva AM, 2013. Hum Immunol. 74:207. PubMed
  13. Koon HW, et al. 2013. J Vis exp. 68:4208. PubMed
  14. Ma Dy, et al. 2013. J Virol. 87:3361. PubMed
  15. Han W, 2013. J. Immunol. 190:4786. PubMed
  16. Garcia-Acros I, et al. 2013. J Biol Chem. 288:14046. PubMed
  17. Zhao JL, et al. 2013. Elife. 21:537. PubMed
  18. Guo W, et al. 2013. J Gerontol A Biol Sci Med Sci. PubMed
  19. Yamasaki S, et al. 2013. Clin Vaccine immunol. 20:1508. PubMed
  20. Kraft P, et al. 2013. Stroke. 44:3202. PubMed
  21. Liu X, et al. 2013. Invest Ophthalmol Vis Sci. 54:7386. PubMed
  22. Fullerton AM, et al. 2013. Toxicol Sci. 136:72. PubMed
  23. Cekic C, et al. 2013. J Exp Med. 210:2693. PubMed
  24. Varga T, et al. 2013. J. Immunol. 191:5695. PubMed
  25. Ma Y, et al. 2014. Cancer Res. 74:436. PubMed
  26. Chang YJ, et al. 2014. Cytotherapy. 16:560. PubMed
  27. Kim MJ, et al. 2014. Mol Pharmacol. 86:284. PubMed
  28. Cekic C, et al. 2014. Cancer Res. 74:7250. PubMed
  29. Szulzewsky F, et al. 2015. PloS One. 10:116644. PubMed
Product Citations
  1. Zhou B, et al. 2020. Nat Immunol. 1.868055556. PubMed
  2. Tan J, et al. 2021. iScience. 24(8):102835. PubMed
  3. Asem M, et al. 2020. Cancer Res. 1156:80. PubMed
  4. Lin C, et al. 2020. Cancer Immunol Res. 632:8. PubMed
  5. Tsai AG, et al. 2020. Nat Med. 408:26. PubMed
  6. Shah D, et al. 2021. Oncoimmunology. 10:1939601. PubMed
  7. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  8. Rathakrishnan A, et al. 2020. Viruses. 12: . PubMed
  9. Wang Y, et al. 2021. Cancer Res. 81:384. PubMed
  10. Wang H, et al. 2022. Nat Biomed Eng. 6:882. PubMed
  11. Yeh PY, et al. 2022. Sens Actuators B Chem. 353: . PubMed
  12. Ammothumkandy A, et al. 2022. Nat Neurosci. 25:493. PubMed
  13. Melendez E, et al. 2022. Development. 149: . PubMed
  14. Argueta LB, et al. 2022. iScience. 25:104223. PubMed
  15. Wang S, et al. 2022. Adv Sci (Weinh). 9:e2201258. PubMed
  16. Meena AS, et al. 2022. Cell Rep. 39:110937. PubMed
  17. Zhang Y, et al. 2022. Vaccines (Basel). 10: . PubMed
  18. Sugimoto S, et al. 2022. Nat Metab. 4:775. PubMed
  19. Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed
  20. Isildar B, et al. 2022. Inflamm Regen. 42:55. PubMed
  21. Xu Q, et al. 2022. Front Med (Lausanne). 9:940554. PubMed
  22. Alexandrou AT, et al. 2022. iScience. 25:105546. PubMed
  23. Wang Y, et al. 2022. Front Endocrinol (Lausanne). 13:1036517. PubMed
  24. He Y, et al. 2022. Front Immunol. 13:986202. PubMed
  25. Tratwal J, et al. 2022. Front Endocrinol (Lausanne). 13:1001210. PubMed
  26. Xie Z, et al. 2022. Cell Discov. 8:133. PubMed
  27. Xiang X, et al. 2022. Medicine (Baltimore). 101:e32558. PubMed
  28. Yu Z, et al. 2023. Cancer Res. 83:700. PubMed
  29. Ding D, et al. 2023. J Med Chem. 66:1467. PubMed
  30. Oh S, et al. 2023. Nat Biotechnol. :. PubMed
  31. Marritt KL, et al. 2023. Front Immunol. 13:1087991. PubMed
  32. He T, et al. 2023. Immun Inflamm Dis. 11:e759. PubMed
  33. Vyas M, et al. 2023. Front Immunol. 13:1098445. PubMed
  34. Li XF, et al. 2023. J Exp Med. 220: . PubMed
  35. Wang J, et al. 2023. Inflamm Regen. 43:12. PubMed
  36. Ma Y, et al. 2022. Am J Physiol Heart Circ Physiol. 322:H622. PubMed
  37. Feng J, et al. 2022. Immunity. 55:405. PubMed
  38. Rustagi Y, et al. 2022. Diabetes. 71:1149. PubMed
  39. Guennoun R, et al. 2022. Cancers (Basel). 14:. PubMed
  40. Zhuo Y, et al. 2022. J Immunother Cancer. 10:. PubMed
  41. Knelson EH, et al. 2022. Cancer Immunol Res. 10:947. PubMed
  42. Wakiyama H, et al. 2022. Cancer Immunol Res. 10:1386. PubMed
  43. Roweth HG, et al. 2022. Sci Adv. 8:eabo5224. PubMed
  44. Zhong J, et al. 2022. J Adv Res. 41:145. PubMed
  45. Yun HJ, et al. 2022. Int J Mol Sci. 23:. PubMed
  46. Daum P, et al. 2023. Front Immunol. 13:991347. PubMed
  47. Rao D, et al. 2023. Eur J Immunol. 53:e2250258. PubMed
  48. Le DT, et al. 2023. iScience. 26:106059. PubMed
  49. Romo-Rodríguez R, et al. 2023. Arch Med Res. 54:197. PubMed
  50. Mabire M, et al. 2023. Nat Commun. 14:1830. PubMed
  51. MacPherson KP, et al. 2023. Front Cell Neurosci. 17:895017. PubMed
  52. Chen Z, et al. 2023. Nat Commun. 14:1839. PubMed
  53. Martins Nascentes Melo L, et al. 2023. J Immunother Cancer. 11:. PubMed
  54. Ramalingam P, et al. 2023. Nat Commun. 14:2018. PubMed
  55. Choi YJ, et al. 2023. Nat Commun. 14:2045. PubMed
  56. He Q, et al. 2023. Parasit Vectors. 16:139. PubMed
  57. Pattnaik A, et al. 2023. Vaccines (Basel). 11:. PubMed
  58. Mulcrone PL, et al. 2023. Sci Rep. 13:8436. PubMed
  59. Villalba N, et al. 2023. J Neuroinflammation. 20:127. PubMed
  60. Song S, et al. 2023. Bioact Mater. 28:358. PubMed
  61. Matsudaira T, et al. 2023. Commun Biol. 6:665. PubMed
  62. Dobie R, et al. 2020. Cell Reports. 29(7):1832-1847.e8.. PubMed
  63. Strickley JD, et al. 2019. Nature. 575:519. PubMed
  64. Deiuliis J, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 302:L399. PubMed
  65. Jin J, et al. 2012. Arterioscler Thromb Vasc Biol. 32:2901. . PubMed
  66. Sandovici I, et al. 2022. Dev Cell. 57:63. PubMed
  67. Barros-Martins J, et al. 2016. J Immunol. 196: 3642 - 3652. PubMed
  68. Jacoby E, et al. 2021. NPJ Regen Med. 6:58. PubMed
  69. Chen Z, et al. 2020. Glia. 68:2148. PubMed
  70. Seow JJW, et al. 2021. Front Med (Lausanne). 8:603374. PubMed
  71. Shin J, et al. 2020. Hum Gene Ther. . PubMed
  72. Bouchareychas L, et al. 2021. iScience. 24(8):102847. PubMed
  73. Pracht K, et al. 2021. Eur J Immunol. 51:1089. PubMed
  74. Radwanska M, et al. 2020. Methods Mol Biol. 2116:721. PubMed
  75. Rendon CJ, et al. 2022. Front Endocrinol (Lausanne). 13:995499. PubMed
  76. Effern M, et al. 2022. STAR Protoc. 3:101038. PubMed
  77. Della Verde G, et al. 2022. Life Sci Alliance. 5:. PubMed
  78. Su C, et al. 2021. Front Oncol. 11:719564. PubMed
  79. Herisson F, et al. 2018. Nat Neurosci. 21:1209. PubMed
  80. Spengler J, et al. 2016. J Infect Dis. 214: S308 - S318. PubMed
  81. Zheng M, et al. 2016. J Immunol. 196: 4122 - 4131. PubMed
  82. St-Pierre S, et al. 2016. PLoS One. 11: 0150230. PubMed
  83. Ensan S, et al. 2015. Nat Immunol. 17: 159-168. PubMed
  84. Jaiswal M, et al. 2015. Sci Rep. 5: 15221. PubMed
  85. Hou J, et al. 2015. J Mol Cell Biol. 7: 338-350. PubMed
  86. Fujii H, et al. 2015. PLoS One. 10: 0133216. PubMed
  87. Garcia-Fabiani MB, et al. 2020. STAR Protoc. :1. PubMed
  88. Zhang J, et al. 2021. Aging (Albany NY). 13:17880. PubMed
  89. Press AT, et al. 2021. EMBO Mol Med. 13:e14436. PubMed
  90. Sima LE, et al. 2021. J Immunother Cancer. 9:. PubMed
  91. Bikorimana JP, et al. 2022. Cell Rep Med. 3:100534. PubMed
  92. Zhang L, et al. 2022. JCI Insight. 7:. PubMed
  93. Baldominos P, et al. 2022. STAR Protoc. 3:101795. PubMed
  94. Chou YJ, et al. 2020. Sci Rep. 10:8422. PubMed
  95. Wang J, et al. 2020. Sci Rep. 10:12785. PubMed
  96. Ma H, et al. 2021. Front Immunol. 12:760095. PubMed
  97. McGill CJ, et al. 2021. STAR Protoc. 2:100948. PubMed
  98. Lutze J, et al. 2022. Methods Mol Biol. 2394:299. PubMed
  99. Hosseini A, et al. 2022. Evid Based Complement Alternat Med. 2022:5639226. PubMed
  100. Liu W, et al. 2017. Int J Environ Res Public Health. 10.3390/ijerph14060592. PubMed
  101. Pietrocola F, et al. 2017. Autophagy. 0.935416667. PubMed
  102. Ma Y, et al. 2014. Cancer Res. 74:436. PubMed
  103. Magalhães L, et al. 2015. PLoS Negl Trop Dis. 10: 0003816. PubMed
  104. Moraes-Vieira P, et al. 2016. Diabetes. 65: 1317 - 1327. PubMed
  105. Johnson CL, et al. 2021. Am J Physiol Heart Circ Physiol. 321:H756. PubMed
  106. Stringhini M, et al. 2021. MAbs. 13:1868066. PubMed
  107. Devalaraja S, et al. 2020. Cell. 1098:180. PubMed
  108. Elisabeth Schrumpf, Xiaojun Jiang 2017. Physiol Rep. 10.14814/phy2.13117. PubMed
  109. Chen J, et al. 2022. Nat Commun. 13:6759. PubMed
  110. Boldin M, et al. 2011. J Exp Med. 208:1189. PubMed
  111. Sun CC, et al. 2020. Genome Med. 0.553472222. PubMed
  112. Bravo–San Pedro JM, et al. 2019. Cell Metab. 30:754. PubMed
  113. Wu Y, et al. 2021. Nat Biomed Eng. 5:1336. PubMed
  114. Meng S, et al. 2022. Exp Anim. . PubMed
  115. Kokkinopoulos I, et al. 2021. Sci Rep. 11:15759. PubMed
  116. Clark EL, et al. 2017. PLoS Genet. 13:e1006997. PubMed
  117. Cantor JR, et al. 2017. Cell. 169:258. PubMed
  118. Ji WJ, et al. 2017. Cytometry B Clin Cytom. 92:371. PubMed
  119. AC Belkina, JE Snyder-Cappione 2017. Cytometry A. 91:175-179. PubMed
  120. Brunmeir R, et al. 2016. PLoS Genet. 12:e1006474. PubMed
  121. Pridans C, et al. 2016. J Immunol. 197: 2297 - 2305. PubMed
  122. Mircheff A, et al. 2016. Ocul Surf. 14:460-483. PubMed
  123. Pennington L, et al. 2016. Nat Commun. 7:11610. PubMed
  124. Koon H, et al. 2012. J Vis Exp. 68: 4208. PubMed
  125. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  126. Zhai Z, et al. 2021. Medicine (Baltimore). 100:e26214. PubMed
  127. Hu D, et al. 2021. J Cell Mol Med. 25:2900. PubMed
  128. Jones N, et al. 2021. Nat Commun. 12:1209. PubMed
  129. Pokhrel RH, et al. 2022. Int J Mol Sci. 23:. PubMed
  130. Sakamoto S, et al. 2020. OncoImmunology. 8(12):e1671760.. PubMed
  131. Rico C, et al. 2012. Carcinogenesis. 33:2283. PubMed
  132. Wang D, et al. 2021. Signal Transduct Target Ther. 6:376. PubMed
  133. Machado H, et al. 2021. PLoS Pathog. 17:e1009933. PubMed
  134. Yan X, et al. 2022. Int J Biol Sci. 18:585. PubMed
  135. Singh SS, et al. 2021. Eur J Med Chem. 216:113312. PubMed
  136. Hamour S, et al. 2015. PLoS One. 10: 0136238. PubMed
  137. Bazett M, Haston M 2016. Sci Rep. 6:19189. PubMed
  138. Luo B, et al. 2016. J Neurosci. 36: 9590 - 9603. PubMed
  139. Patera A, et al. 2016. J Leukoc Biol. 100(6):1239-1254. PubMed
  140. Jeong J, et al. 2021. Immune Netw. 21:e31. PubMed
  141. Sitaraman S, et al. 2021. JCI Insight. 6:. PubMed
  142. Orr AN, et al. 2021. Int J Mol Sci. 22:. PubMed
  143. Mo L, et al. 2022. Front Oncol. 12:871489. PubMed
  144. Saraiva A, et al. 2013. Hum Immunol. 74:207. PubMed
  145. Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed
  146. Huang L, et al. 2021. J Cell Physiol. 236:7308. PubMed
  147. Whytock KL, et al. 2022. iScience. 25:104772. PubMed
  148. Neufeld L, et al. 2021. Sci Adv. 7:. PubMed
  149. Friedman G, et al. 2020. Nat Cancer. 1:692. PubMed
  150. Kumar A, et al. 2021. Part Fibre Toxicol. 18:43. PubMed
  151. Tanaka Y, et al. 2021. Int J Mol Sci. 22:. PubMed
  152. Scherer SD, et al. 2021. Breast Cancer Res. 23:100. PubMed
  153. Jiang J, et al. 2018. Cancer Med. 3.504861111. PubMed
  154. Chen Z, et al. 2017. Cancer Res. 77:2266. PubMed
  155. Kim M, et al. 2017. Int J Mol Sci. 10.3390/ijms18061275. PubMed
  156. Facchini M, et al. 2014. J Vis Exp. 85: 51019. PubMed
  157. Ilievski V, et al. 2015. PLoS One. 10: 0136502. PubMed
  158. Kildisiute G, et al. 2021. Sci Adv. 7: . PubMed
  159. Schiferle EB, et al. 2021. Sci Adv. 7: . PubMed
  160. Devalaraja S, et al. 2020. STAR Protoc. 1:100188. PubMed
  161. Bigenwald C, et al. 2021. Nat Med. 27:851. PubMed
  162. Gingrich AA, et al. 2021. Front Immunol. 12:670309. PubMed
  163. Qin L, et al. 2022. Nat Commun. 13:6051. PubMed
  164. Escudero-Lara A, et al. 2022. Front Behav Neurosci. 16:946975. PubMed
  165. Patil R, et al. 2019. J Biomed Opt. 24:1. PubMed
  166. Ongaro T, et al. 2020. Oncotarget. 3.026388889. PubMed
  167. Kahlenberg J, et al. 2011. J Immunol. 187:6143. PubMed
  168. Han W, et al. 2013. J Immunol. 190:4786. PubMed
  169. Dang Y, et al. 2022. Cancer Cell Int. 22:88. PubMed
  170. Deng J, et al. 2021. Breast Cancer Res Treat. 185:85. PubMed
  171. Vivanco Gonzalez N, et al. 2022. STAR Protoc. 3:101280. PubMed
  172. Wang K, et al. 2017. Molecular Immunology. 10.1016/j.molimm.2017.04.016. PubMed
  173. Appelbe O, et al. 2017. J Control Release.. 10.1016/j.jconrel.2017.09.043. PubMed
  174. Tang J, et al. 2017. Proc Natl Acad Sci U S A. 114:E7441. PubMed
  175. Liccardi G, et al. 2019. Mol Cell. 73:413. PubMed
  176. Yamasaki S, et al. 2013. Clin Vaccine Immunol. 20:1508. PubMed
  177. Fukui R, et al. 2016. J Immunol. 196: 416 - 427. PubMed
  178. Rodriguez-Garcia M, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.72. PubMed
  179. Judge SJ, et al. 2020. J Clin Invest. 130:3051. PubMed
  180. Choi JW, et al. 2020. PLoS One. 15:e0235518. PubMed
  181. Alban TJ, et al. 2020. Front Immunol. 1.285416667. PubMed
  182. Jiang S, et al. 2017. Toxicology Lett. 10.1016/j.toxlet.2017.03.015. PubMed
  183. Martínez-Muriana A, et al. 2020. Stem Cell Res Ther. 11:53. PubMed
  184. Pennig J, et al. 2019. Sci Rep. 9:17937. PubMed
  185. Zhang L, et al. 2021. Methods Mol Biol. 2388:175. PubMed
  186. Evans AK, et al. 2020. Neurobiol Dis. 146:105089. PubMed
  187. Wang B, et al. 2017. Stem Cell Res Ther.. 10.1186/s13287-017-0744-6. PubMed
  188. Anstine L,et al. 2017. J Am Heart Assoc.. 10.1161/JAHA.117.007097. PubMed
  189. Agrawal V, et al. 2016. J Leukoc Biol. 100: 261 - 274. PubMed
  190. Cheng Q, et al. 2020. Nat Nanotechnol. 0.842361111. PubMed
  191. Xie Z, et al. 2020. Light Sci Appl. 0.486805556. PubMed
  192. Alghamri MS, et al. 2020. Methods Enzymol. 632:215. PubMed
  193. Yu Z, et al. 2021. Front Oncol. 11:659251. PubMed
  194. Deng J, et al. 2021. Nat Cancer. 2:503. PubMed
  195. Nwani NG, et al. 2019. Cancers (Basel). 0.806944444. PubMed
  196. Ramalingam P, et al. 2020. Nat Commun. 11:666. PubMed
  197. Garcia-Arcos I, et al. 2013. J Biol Chem. 288:14046. PubMed
  198. Beaudoin-Bussières G, et al. 2022. Cell Rep. 38:110368. PubMed
  199. Hu H, et al. 2021. Cancer Immunol Res. 9:1298. PubMed
  200. Zhong W, et al. 2022. Nat Commun. 13:4390. PubMed
  201. So EY, et al. 2021. Am J Physiol Cell Physiol. 321:C569. PubMed
  202. Bunting MD, et al. 2022. Sci Adv. 8:eabk3327. PubMed
  203. Elphinstone R, et al. 2017. Infect Immun. 10.1128/IAI.00371-17. PubMed
  204. Kernfeld EM, et al. 2018. Immunity. 48:1258. PubMed
  205. Sci T 2013. Toxicol Sci. 136:72. PubMed
  206. Metz H, et al. 2016. Proc Natl Acad Sci U S A. 113: 8795 - 8800. PubMed
  207. Patel M, et al. 2021. Cancer Res. 81:3985. PubMed
  208. Shi X, et al. 2021. Nat Commun. 12:2547. PubMed
  209. Ramaswamy G, et al. 2017. Sci Rep. 10.1038/srep45140. PubMed
  210. Mirando AC, et al. 2020. Oncoimmunology. 9:1760685. PubMed
  211. Rosenbluth JM, et al. 2020. Nat Commun. 11:1711. PubMed
  212. Tavakol DN, et al. 2021. Curr Protoc. 1:e275. PubMed
  213. Ullah I, et al. 2021. Immunity. 54:2143. PubMed
  214. He Y, et al. 2021. Front Immunol. 12:641206. PubMed
  215. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  216. Ercoli G, et al. 2022. Clin Transl Immunology. 11:e1366. PubMed
  217. Meng YM, et al. 2021. Nat Commun. 12:6011. PubMed
  218. Zhu QY, et al. 2021. Nat Commun. 12:6624. PubMed
  219. Lizotte P, et al. 2016. Sci Rep. 6:31745. PubMed
  220. Sharma A, et al. 2020. Cell. 183(2):377-394.e21. PubMed
  221. Fidalgo MF, et al. 2022. Life Sci Alliance. 5:. PubMed
  222. Stirling ER, et al. 2022. J Immunother Cancer. 10:. PubMed
  223. Hang J, et al. 2019. Cancer Med. 1.254166667. PubMed
  224. Tan X, et al. 2019. Sci Rep. 2.7125. PubMed
  225. Lienemann PS, et al. 2020. Adv Sci (Weinh). 7:1903395. PubMed
  226. Zhao J, et al. 2013. Elife. 21:537. PubMed
  227. Hills LB, et al. 2021. J Immunol. 206:89. PubMed
  228. Page N, et al. 2021. Nat Commun. 12:1009. PubMed
  229. Pace J, et al. 2022. J Biomed Opt. 27:. PubMed
  230. Pedicona F, et al. 2022. Sci Signal. 15:eabl7989. PubMed
  231. Wen J, et al. 2017. J Hematol Oncol. 10.1186/s13045-017-0489-9. PubMed
  232. Ahmad S,et al. 2017. Prog Neuropsychopharmacol Biol Psychiatry. . 10.1016/j.pnpbp.2017.10.001. PubMed
  233. Weber G, et al. 2015. Surgery. 158: 248-254. PubMed
  234. Jiang H, et al. 2016. Nat Med. 10.1038/nm.4123. PubMed
  235. Elgueta R, et al. 2016. J Immunol. 197: 3970 - 3981. PubMed
  236. Zhao D, et al. 2020. Cancer Discov. 10:1374. PubMed
  237. Castoldi F, et al. 2020. Cell Death Discov. 0.339583333. PubMed
  238. Wang L, et al. 2020. EMBO J. 39:e104514. PubMed
  239. Morris C, et al. 2017. MBio. 10.1128/mBio.00036-17. PubMed
  240. Yan Q, et al. 2022. Clin Transl Med. 12:e1084. PubMed
  241. Schweppe RE, et al. 2019. Mol Cancer Res. 17:1036. PubMed
  242. Yamada KJ, et al. 2020. PLoS Pathog. 16:e1008354. PubMed
  243. Zhai K, et al. 2021. Nat Cancer. 2:1136. PubMed
  244. Yang A, et al. 2018. Cancer Discov. 0.525. PubMed
  245. Rentsendorj A, et al. 2017. Brain Behav Immun. 10.1016/j.bbi.2017.08.019. PubMed
  246. Theurl I, et al. 2016. Nat Med. 10.1038/nm.4146. PubMed
  247. Nowyhed H, et al. 2017. Sci Rep. 7:40273. PubMed
  248. De Domenico E, et al. 2020. STAR Protoc. 1:100233. PubMed
  249. Wang Z, et al. 2020. Cell Rep. 33:108472. PubMed
  250. Sadik A, et al. 2020. Cell. 1252:182. PubMed
  251. Hiza H, et al. 2021. Sci Rep. 11:13190. PubMed
  252. Ito T, et al. 2021. Nat Commun. 12:2232. PubMed
  253. Michelotti TC, et al. 2022. Front Cell Dev Biol. 10:1025240. PubMed
  254. Ren X, et al. 2022. STAR Protoc. 3:101818. PubMed
  255. Yang YS, et al. 2020. Mol Ther Methods Clin Dev. 17:922. PubMed
  256. Gangwar RS, et al. 2020. Sci Rep. 10:14348. PubMed
  257. Hollerbach A, et al. 2021. J Thromb Haemost. . PubMed
  258. Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed
  259. Yang Z, et al. 2021. Nat Commun. 12:4852. PubMed
  260. Zhang Y, et al. 2022. Vaccines (Basel). 10: . PubMed
  261. Majumdar D, et al. 2021. Curr Protoc. 1:e216. PubMed
  262. Dyck S, et al. 2018. J Neuroinflammation. 0.6875. PubMed
  263. Varga T, et al. 2013. J Immunol. 191:5695. PubMed
  264. Samuelson D, et al. 2016. J Immunol. 196: 2655 - 2665. PubMed
  265. Tsao P, et al. 2016. Proc Natl Acad Sci U S A. 113: 8242 - 8247. PubMed
  266. Soncini M, et al. 2016. Proc Natl Acad Sci U S A. 113: E6219 - E6227. PubMed
  267. Yang C, et al. 2016. Sci Rep. 6:34643. PubMed
  268. Zhang J, et al. 2020. Pain. 161:1381. PubMed
  269. Heim CE, et al. 2020. Nature Microbiology. 5(10):1271-1284. PubMed
  270. Feng D, et al. 2022. Nat Commun. 13:5554. PubMed
  271. Gladow N, et al. 2020. PLoS One. 15:e0227734. PubMed
  272. Ramachandran P, et al. 2019. Nature. 575:512. PubMed
  273. Saber M, et al. 2021. J Neurosci Res. 99:1136. PubMed
  274. Xiang Y, et al. 2022. Skelet Muscle. 12:19. PubMed
  275. Yamazaki T, et al. 2021. Oncoimmunology. 10:1962592. PubMed
  276. Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed
  277. Bowers J, et al. 2017. Front Immunol. 10.3389/fimmu.2017.01221. PubMed
  278. Canali S, et al. 2017. Blood. 129(4):405-414. PubMed
  279. Shen X, et al. 2016. Sci Rep. 6:38778. PubMed
  280. Sauter K, et al. 2016. Am J Physiol Gastrointest Liver Physiol. 311: G533 - G547. PubMed
  281. Song C, et al. 2015. Blood. 126: 1813 - 1822. PubMed
  282. Sasaki Y, et al. 2015. PLoS One. 10: 0131176. PubMed
  283. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  284. Tulasi DY, et al. 2021. Cell Mol Gastroenterol Hepatol. 11:1437. PubMed
  285. Hoffmann J, et al. 2021. Nat Commun. 12:3964. PubMed
  286. Yang YS, et al. 2022. Nat Commun. 13:6175. PubMed
  287. Ma D, et al. 2013. J Virol. 87:3361. PubMed
  288. Xue X, et al. 2021. Cent Eur J Immunol. 46:191. PubMed
  289. Unsinger J, et al. 2021. J Leukoc Biol. 109:697. PubMed
  290. Ansari M, et al. 2017. Cell Signal. 10.1016/j.cellsig.2017.04.014. PubMed
  291. Ahmad S, et al. 2017. Prog Neuropsychopharmacol Biol Psychiatry. 10.1016/j.pnpbp.2017.06.034. PubMed
  292. Bichsel C, et al. 2017. Sci Rep. 10.1038/s41598-017-09928-1. PubMed
  293. James M, et al. 2017. J Nucl Med. . 10.2967/jnumed.117.189597. PubMed
  294. Annibaldi A, et al. 2018. Mol Cell. 69:566. PubMed
  295. Hill SJ, et al. 2018. Cancer Discov. 1.308333333. PubMed
  296. Kraft P, et al. 2013. Stroke. 44:3202. PubMed
  297. Kim M, et al. 2014. Mol Pharmacol. 86:284. PubMed
  298. Cekic C, et al. 2014. Cancer Res. 74:7250. PubMed
  299. Pankova D, et al. 2016. Mol Cancer Res. 14: 287 - 295. PubMed
  300. Mai CL, et al. 2021. Theranostics. 11:1059. PubMed
  301. Chen P, et al. 2021. Clin Transl Med. 11:e462. PubMed
  302. Peng X, et al. 2021. Stem Cell Res Ther. 198:12. PubMed
  303. Linder A, et al. 2020. EMBO J. 39:e105071. PubMed
  304. Li N, et al. 2022. Nat Commun. 13:7281. PubMed
  305. Hsia B, et al. 2012. J Allergy Clin Immunol. 130:205. PubMed
  306. Resende M, et al. 2010. Physiol Genomics. 42:437. PubMed
  307. McLaughlin S, et al. 2019. Nat Commun. 3.795833333. PubMed
  308. Koury J, et al. 2019. PLoS One. 14:e0218670. PubMed
  309. Soileau AM, et al. 2022. Vet Sci. 9:. PubMed
  310. McClellan BL, et al. 2022. STAR Protoc. 3:101357. PubMed
  311. Governa V, et al. 2022. Proc Natl Acad Sci U S A. 119. PubMed
  312. Deng Y, et al. 2021. Nat Commun. 12:7041. PubMed
  313. Zhang Z, et al. 2017. Journal of Translational Medicine. 10.1186/s12967-017-1196-6. PubMed
  314. Muñoz-Ruiz M, et al. 2016. Nat Immunol. 10.1038/ni.3424. PubMed
  315. Chang Y, et al. 2014. Cytotherapy. 16:560. PubMed
  316. Ubellacker J, et al. 2017. Breast Cancer Res. 10.1186/s13058-017-0815-8. PubMed
  317. DU T, et al. 2020. Front Immunol. 1.571527778. PubMed
  318. Trial J, et al. 2020. Geroscience. . PubMed
  319. Sobecki M, et al. 2022. Cell Stem Cell. 29:1459. PubMed
  320. Cordo' V, et al. 2022. Nat Commun. 13:1048. PubMed
  321. Hallisey M, et al. 2022. STAR Protoc. 3:101163. PubMed
  322. Denoth-Lippuner A, et al. 2021. Cell Stem Cell. 28:2020. PubMed
  323. Maas RR, et al. 2021. Nat Protoc. 16:4692. PubMed
  324. Nagree MS, et al. 2022. EMBO Mol Med. 14:e14297. PubMed
  325. Mochizuki M,et al. 2017. J Am Heart Assoc. . 10.1161/JAHA.117.005920. PubMed
  326. Roh–Johnson M, et al. 2017. Dev Cell. 43:549. PubMed
  327. Müller C, et al. 2018. Elife. 7:e34985. PubMed
  328. Guo W, et al. 2013. J Gerontol A Biol Sci Med Sci. . PubMed
  329. Liu Y, et al. 2015. Sci Rep. 5: 15134. PubMed
  330. Hodson D, et al. 2016. Proc Natl Acad Sci U S A. 113: 2039-2046. PubMed
  331. Munson D, et al. 2016. Proc Natl Acad Sci U S A. 113: 8272 - 8277. PubMed
  332. Vechetti IJ, et al. 2021. FASEB J. 35:e21644. PubMed
  333. Saber M, et al. 2020. Eur J Neurosci. 52:2791. PubMed
  334. Wu R, et al. 2020. J Immunother Cancer. 8:00. PubMed
  335. Visweshwaran SP, et al. 2022. Front Cell Dev Biol. 10:1008898. PubMed
  336. Wu C, et al. 2012. PLoS One. 7:e42363. PubMed
  337. Denning W, et al. 2011. PLoS One. 6:e16897. PubMed
  338. Li Q, et al. 2022. Signal Transduct Target Ther. 7:222. PubMed
  339. Fielding C, et al. 2022. Nat Commun. 13:543. PubMed
  340. Weaver J, et al. 2017. Part Fibre Toxicol. 10.1186/s12989-017-0206-4. PubMed
  341. Samuelson D, et al. 2017. PLoS Pathogens. 13(6):e1006426. PubMed
  342. Wong C, et al. 2017. J Immunol. 10.4049/jimmunol.1700397. PubMed
  343. Yu H, et al. 2016. Sci Rep. 6:35190. PubMed
  344. Now H, Yoo J 2016. Cell Mol Immunol. 10.1038/cmi.2016.32. PubMed
  345. Bosma M, et al. 2016. Nat Commun. 7: 11314. PubMed
  346. YC S, SJ L 2015. Sci Rep. 5: 12578. PubMed
  347. Szulzewsky F, et al. 2015. PLoS One. 10:116644. PubMed
  348. Barcia Durán JG, et al. 2021. Commun Biol. 4:406. PubMed
  349. Park HJ, et al. 2021. Int J Mol Sci. 22:. PubMed
  350. Cebrian-Silla A, et al. 2021. Elife. 10:. PubMed
  351. Shaashua L, et al. 2022. Nat Commun. 13:6513. PubMed
  352. Gupta R, et al. 2020. Cell Stem Cell. 26:527. PubMed
  353. Roybal J, et al. 2011. Mol Cancer Res. 9:25. PubMed
  354. Yeini E, et al. 2021. Nat Commun. 12:1912. PubMed
  355. Wang X, et al. 2021. Sci Transl Med. 13:. PubMed
  356. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  357. Kucykowicz S, et al. 2022. STAR Protoc. 3:101356. PubMed
  358. Kim TW, et al. 2022. Mol Ther Oncolytics. 26:1. PubMed
  359. Kathania M, et al. 2022. Cell Rep. 40:111345. PubMed
  360. Cekic C, et al. 2013. J Exp Med. 210:2693. PubMed
  361. Xie Z, et al. 2020. Light Sci Appl. 9:161. PubMed
  362. Huang N, et al. 2021. Nat Med. 27:892. PubMed
  363. Song X, et al. 2021. FEBS Open Bio. 11:2453. PubMed
  364. Chen S, et al. 2020. Cancer Immunol Res. 1064:8. PubMed
  365. Erady C, et al. 2021. NPJ Genom Med. 6:04. PubMed
  366. de Silva S, et al. 2020. Cancer Immunol Res. 0.493055556. PubMed
  367. Wan C, et al. 2021. Cancer Res. 81:158. PubMed
  368. Field-Corbett C, et al. 2009. Stem Cells. 27:662. PubMed
  369. Zeng Y, et al. 2019. FASEB J. 33:6596. PubMed
  370. Zheng W, et al. 2019. Cell Rep. 29:1747. PubMed
  371. Tomas C, et al. 2019. Sci Rep. 9:11464. PubMed
  372. Kim JK, et al. 2019. Bioengineering (Basel). 6:24. PubMed
  373. Roy IM, et al. 2021. Bio Protoc. 11:e4130. PubMed
  374. Alam A, et al. 2022. STAR Protoc. 3:101563. PubMed
  375. Xiang Y, et al. 2022. Exp Mol Med. 54:1011. PubMed
  376. Guo Z, et al. 2022. Neurobiol Pain. 12:100096. PubMed
  377. Guo P, et al. 2022. Nat Cell Biol. 24:99. PubMed
  378. Wahane S, et al. 2021. Sci Adv. 7:. PubMed
  379. Alizadeh A, et al. 2018. J Neuroinflammation. 15:53. PubMed
  380. Guo P, et al. 2017. J Clin Invest. 127:4242. PubMed
  381. Tsai SC, et al. 2017. Biomicrofluidics. 11:034122. PubMed
  382. Wang X, et al. 2016. J Virol. 90: 10247 - 10258. PubMed
  383. Mohammed R, et al. 2016. Cell Rep. 14:760-771. PubMed
  384. Durward-Diioia M, et al. 2015. Infect Immun . 83: 4759 - 4771. PubMed
  385. Birnie K, et al. 2015. Mol Cancer Res. 13: 1106 - 1118. PubMed
  386. Liu X, et al. 2013. Invest Ophthalmol Vis Sci . 54:7386. PubMed

Antigen Details

Cell Type
Tregs
Gene ID
NA
Go To Top Version: 3    Revision Date: 06.10.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account