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APC anti-human BLNK Phospho (Tyr96) Antibody

Pricing & Availability
Clone
A19008A (See other available formats)
Regulatory Status
RUO
Other Names
B Cell Linker, SLP-65, BLNK-S, BASH
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A19008A_APC_AhR_Anibody_1_081825
Human peripheral blood lymphocytes were treated with (left) or without (right) 12 µg/mL of anti-human IgG, IgM antibody (Cat. No 397302) for 5 minutes. Cells were then fixed with Fixation Buffer (Cat. No. 420801), permeabilized with True-Phos™ Perm Buffer (Cat. No. 425401) and stained intracellularly with anti-human CD20 (clone 1412) FITC and anti-human BLNK Phospho (Tyr96) (clone A19008A) APC.
  • A19008A_APC_AhR_Anibody_1_081825
    Human peripheral blood lymphocytes were treated with (left) or without (right) 12 µg/mL of anti-human IgG, IgM antibody (Cat. No 397302) for 5 minutes. Cells were then fixed with Fixation Buffer (Cat. No. 420801), permeabilized with True-Phos™ Perm Buffer (Cat. No. 425401) and stained intracellularly with anti-human CD20 (clone 1412) FITC and anti-human BLNK Phospho (Tyr96) (clone A19008A) APC.
See APC spectral data
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382205 25 tests 206 CHF
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382206 100 tests 429 CHF
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Description

B cell linker protein (BLNK), a member of the SLP-76 family, is an adapter molecule that plays important roles in B cell antigen receptor (BCR) engagement and B cell activation. Cross-linking of the BCR stimulates various downstream signaling pathways involved in cell proliferation and apoptosis. Intracellular protein tyrosine kinases such as Syk and Lyn have been implicated in BCR signal transduction and are thought to play an important role in B cell development. Phosphorylation of human BLNK at tyrosines 84, 178, and 189 (Y84, Y178, and Y189) creates docking sites for effector proteins GRB2, Vav, Nck and PLCγ2, which in turn leads to the activation of downstream signaling pathways. Phosphorylation of tyrosine 96 (Tyr96) facilitates BTK binding and plays a critical role in B cell maturation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide of human BLNK (phosphorylated at Tyr 96)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool).
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)

Antigen Details

Structure
Human BLNK is 456 amino acids (aa) in length and it contains a Pro­rich region (aa 98­260) and one SH2 domain (aa 346­453).
Distribution

Cytosol, plasma-membrane associated, B cells

Function
Links B cell receptor engagement with downstream signaling events
Interaction
PLCG1, PLCG2, VAV1, NCK1, VAV3, GRB2, FYN, LYN, SYK
Molecular Family
Phospho-Proteins
Antigen References
  1. Fu C, et al. 1998. Immunity 9:93-103.
  2. Ishiai M, et al. 1999. Immunity 10:117-25.
  3. Baba Y, et al. 2001. Proc. Natl. Acad. Sci. USA 98:2582-6.
  4. Tsuji S, et al. 2001. J. Exp. Med. 194:529-39.
  5. Chiu CW, et al. 2002. EMBO. J. 21:6461-72.
  6. Taguchi T, et al. 2004. Immunology. 122:575-82.
Gene ID
29760 View all products for this Gene ID
UniProt
View information about BLNK Phospho Tyr96 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 07.31.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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