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Brefeldin A Solution (1,000X)

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Regulatory Status
RUO
Other Names
BFA
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420601 1 mL 65 CHF
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Description

Brefeldin A (BFA) is a protein transport inhibitor commonly used to enhance intracellular cytokine staining signals by blocking transport processes during cell activation. Especially useful for the intracellular staining of cytokines, BFA leads to the accumulation of most cytokines at the Golgi Complex/Endoplasmic Reticulum (see Jung, et al., 1993). Optimal conditions for use are cell type and time-dependent. Typically, protein transport inhibitors are included during in vitro cell activation cultures for 4-24 hours prior to harvest (see references below for additional information). Brefeldin A Solution is supplied as a 1,000X solution, which should be diluted to 1X in cell culture medium.

Product Details
Technical Data Sheet (pdf)

Product Details

Formulation
Brefeldin A Solution is supplied as a 1000X in DMSO, which should be diluted to 1X in cell culture medium.
Concentration
5.0 mg/ml
Storage & Handling
Store the Brefeldin A Solution (1,000X) between 2°C and 8°C. Note: DMSO freezes at this temperature.
Application

ICFC - Quality tested
Recommended Usage

Dilute the 1000X solution to 1X in the tissue culture medium. It is recommended that cells are cultured with brefeldin A for ≤ 24 hours, as this can become toxic for cell viability.

Application Notes

Completely thaw the solution before use, DMSO freezes at 4°C.  We recommend aliquotting the reagent into smaller volumes to avoid repeated freeze-thawing.  

Application References

(PubMed link indicates BioLegend citation)
  1. O'Sullivan BJ, et al. 2006. J. Immunol. 176:7278.
  2. Fuse S, et al. 2007. J. Immunol. 178:5227.
  3. Kang YJ, et al. 2007. Nature Immunol. 8:601.
  4. Redfern CH,et al.2006.J. Clin Oncol.19:3107. PubMed
  5. Smithey MJ, et al. 2008. J. Immunol. 180:3406. PubMed
  6. Elzey BD, et al. 2008. Blood 111:38684. PubMed
  7. Nguyen KD, et al. 2008. J. Immunol. 181:5386. PubMed
  8. Miner MD, et al. 2008. Microb Pathog. 45:273. PubMed
  9. Goodridge HS, et al. 2009. J. Immunol. 182:1146. PubMed
  10. Markey KA, et al. 2009. Blood 113:5644. PubMed
  11. Wilcox RA, et al. 2009. Blood PubMed
  12. Zenaro E, et al. 2009. J. Leukoc Bio. PubMed
  13. Rozkova D, et al. 2009. Clin Immunol. 131:1. PubMed
  14. Butcher JP, et al. 2010. Clin Cancer Res. 16:2065.
  15. Boisvert M, et al. 2010. J. Virol. 84:7782. PubMed
  16. Liu WC, et al. 2010. J. Virol. 84:12011.15. Tawara I, et al. 2011. Clin Cancer Res. 17:77. PubMed
  17. Iwata Y, et al. 2011. Blood. 117:530. PubMed
  18. Schmidt NW, et al. 2011. J. Immunol. 186:3836. PubMed
  19. Langkjaer A, et al. 2012. Clin Immunol. 145:1. PubMed
  20. Molinero LL, et al. 2012. PNAS. 109:18529. PubMed
  21. Li HW, et al. 2012. J. Immunol. 189:5572. PubMed
  22. Wang JM, et al. 2013. J. Virol. 87:4372. PubMed
  23. Fukuoka A, et al. 2013. Int Immunol. 25:373. PubMed
  24. Wang Jm, et al. 2013. J Virol. 87:11626. PubMed
  25. Lee TH, et al. 2013. J Biol Chem. 288:32873. PubMed
  26. Shi L, et al. 2014. J. Immunol. 192:649. PubMed
  27. Bulati M, et al. 2014. Exp Gerontol. PubMed
  28. Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed
  29. Tuna H, et al. 2014. J Leukoc Biol. 95:471. PubMed
  30. Miller ML, et al. 2014. PNAS. 111:7397. PubMed
  31. Wang Y, et al. 2014. Sci Rep. 4:5346. PubMed
  32. Zhou GL, et al. 2014. J Cell Sci. 127:3916. PubMed
  33. Rist MJ, et al. 2015. J Virol. 89:703. PubMed
Product Citations
  1. Wang Y, et al. 2014. Sci Rep. 4:5346. PubMed
  2. Morse M, et al. 2016. Lupus. 25: 81 - 87. PubMed
  3. Li B, Schmidt N 2016. PLoS One. 11: 0162427. PubMed
  4. Rappazzo CG, et al. 2020. bioRxiv. . PubMed
  5. Yakkala C, et al. 2021. Front Immunol. 12:695150. PubMed
  6. Zheng B, et al. 2021. Int J Mol Sci. 22: . PubMed
  7. Alexander KL, et al. 2021. Gastroenterology. 161:522. PubMed
  8. Trinks N, et al. 2021. Commun Biol. 4:1151. PubMed
  9. Wang R, et al. 2018. Oncoimmunology. 7:e1461303. PubMed
  10. Persaud AT, et al. 2022. J Leukoc Biol. 111:573. PubMed
  11. Yu L, et al. 2022. FEBS J. 289:3241. PubMed
  12. Ritzel RM, et al. 2022. Geroscience. 44:1407. PubMed
  13. D'Angeli V, et al. 2022. Eur J Immunol. 52:1058. PubMed
  14. Lu R, et al. 2022. Scand J Immunol. 96:e13177. PubMed
  15. Kanbar JN, et al. 2022. J Exp Med. 219: . PubMed
  16. Minichino D, et al. 2022. J Leukoc Biol. 112:1089. PubMed
  17. Gao KM, et al. 2022. Proc Natl Acad Sci U S A. 119:e2202327119. PubMed
  18. Liu H, et al. 2022. Cell Rep Med. 3:100660. PubMed
  19. Jondle CN, et al. 2022. J Virol. 96:e0063922. PubMed
  20. Blanas A, et al. 2022. iScience. 25:104719. PubMed
  21. Hsieh T, et al. 2022. Front Immunol. 13:901030. PubMed
  22. Li FL, et al. 2022. EMBO J. 41:e110698. PubMed
  23. Bates TA, et al. 2022. Cell Rep. 41:111544. PubMed
  24. Dutt TS, et al. 2022. Cell Rep. 41:111783. PubMed
  25. Phares TW, et al. 2022. Front Pharmacol. 13:1029636. PubMed
  26. Shamriz O, et al. 2023. Front Immunol. 13:1044933. PubMed
  27. Chan L, et al. 2022. Cells. 12: . PubMed
  28. Chan L, et al. 2023. Int J Mol Sci. 24: . PubMed
  29. Nanishi E, et al. 2023. NPJ Vaccines. 8:18. PubMed
  30. Hajjar S, et al. 2022. Cell Death Differ. 29:585. PubMed
  31. Li Y, et al. 2022. Brain Behav Immun. 101:1. PubMed
  32. Zander R, et al. 2022. Immunity. 55:475. PubMed
  33. Pymm P, et al. 2022. Cell Rep. 38:110449. PubMed
  34. Giles JR, et al. 2022. Immunity. 55:557. PubMed
  35. Ye L, et al. 2022. Cell Metab. 34:595. PubMed
  36. Guo H, et al. 2022. Biomaterials. 283:121441. PubMed
  37. Zhao Y, et al. 2022. ACS Appl Mater Interfaces. 14:21872. PubMed
  38. Bronge M, et al. 2022. Sci Adv. 8:eabn1823. PubMed
  39. Sun Y, et al. 2022. STAR Protoc. 3:101479. PubMed
  40. Redelman-Sidi G, et al. 2022. Cancer Immunol Res. 10:1241. PubMed
  41. Frost JN, et al. 2022. Sci Adv. 8:eabq5384. PubMed
  42. Lao L, et al. 2023. Cancer Immunol Res. 11:320. PubMed
  43. Zhao Q, et al. 2023. Signal Transduct Target Ther. 8:40. PubMed
  44. Yang WL, et al. 2023. Nat Commun. 14:863. PubMed
  45. Wu Z, et al. 2023. EMBO Rep. 24:e56524. PubMed
  46. Li G, et al. 2023. Cell Death Dis. 14:185. PubMed
  47. Kozlovski S, et al. 2023. Front Immunol. 13:1041552. PubMed
  48. Mohammadpour H, et al. 2023. Cell Rep. 42:112250. PubMed
  49. Jung HY, et al. 2023. Oncoimmunology. 12:2190671. PubMed
  50. Li Y, et al. 2023. Vaccines (Basel). 11:. PubMed
  51. Onyshchenko K, et al. 2023. Nat Commun. 14:2087. PubMed
  52. Zhou X, et al. 2023. Sci Rep. 13:6701. PubMed
  53. Ko ES, et al. 2023. Cell Commun Signal. 21:107. PubMed
  54. Wang Y, et al. 2023. iScience. 26:106630. PubMed
  55. Thomas OG, et al. 2023. Sci Adv. 9:eadg3032. PubMed
  56. Changsheng H, et al. 2023. Sci Adv. 9:eade4186. PubMed
  57. Adamo S, et al. 2023. Cell Host Microbe. 31:928. PubMed
  58. LaPorte KM, et al. 2023. J Immunother Cancer. 11:. PubMed
  59. Patskovsky Y, et al. 2023. Nat Commun. 14:3763. PubMed
  60. Chen J, et al. 2022. Adv Healthc Mater. 11:e2101714. PubMed
  61. Pablo-Bernal R, et al. 2016. J Gerontol A Biol Sci Med Sci. 71: 610 - 618. PubMed
  62. Tuna H, et al. 2014. J Leukoc Biol. 95:471. PubMed
  63. Aguilar-Pineda JA, et al. 2021. iScience. 24:102993. PubMed
  64. Moudra A, et al. 2021. J Immunol. 206:2109. PubMed
  65. Qian Y, et al. 2021. Cell Reports. 36(8):109602. PubMed
  66. Zhu S, et al. 2017. Exp Cell Res.. 10.1016/j.yexcr.2017.10.013. PubMed
  67. Li L,et al. 2017. Sci Rep.. 10.1038/s41598-017-14000-z. PubMed
  68. Xin G, et al. 2021. Cancer Immunol Res. 9:454. PubMed
  69. Rodrigues M, et al. 2022. Biochem J. 479:1891. PubMed
  70. Nesterenko PA, et al. 2021. Cell Rep. 37:110167. PubMed
  71. Lefebvre MN, et al. 2021. Cell Rep. 37:109956. PubMed
  72. Muliaditan T, et al. 2021. Cell Rep Med. 2:100457. PubMed
  73. Fote GM, et al. 2022. J Cell Sci. 135:. PubMed
  74. Shi L, et al. 2014. J Immunol. 192:649. PubMed
  75. Weng RR, et al. 2021. Nat Commun. 12:2163. PubMed
  76. Yamamoto A, et al. 2019. J Clin Invest. 129:3640. PubMed
  77. Maschmeyer P, et al. 2017. J Autoimmun.. 10.1016/j.jaut.2017.11.005. PubMed
  78. Kiritsy MC, et al. 2021. Elife. 10:. PubMed
  79. Just S, et al. 2016. Sci Rep. 6:39796. PubMed
  80. Gay MD, et al. 2020. Cancer Prev Res (Phila). . PubMed
  81. Piper CJM, et al. 2020. Cell Reports. 29(7):1878-1892.e7.. PubMed
  82. Liang Q, et al. 2022. iScience. 25:104043. PubMed
  83. Cook CP, et al. 2022. Cell Rep Med. 3:100715. PubMed
  84. Snyder LM, et al. 2021. PLoS Pathog. 17:e1009970. PubMed
  85. Krone A, et al. 2022. Sci Rep. 218:. PubMed
  86. Michaud D, et al. 2022. Front Immunol. 12:745873. PubMed
  87. Nagatake T, et al. 2022. Mucosal Immunol. 15:289. PubMed
  88. Starnbach X 2015. J Immunol. 195: 1665-1675. PubMed
  89. Garg B, et al. 2016. Mutagenesis. 10.1093/mutage/gew023. PubMed
  90. Ruhland M, et al. 2016. Nat Commun. 7:11762. PubMed
  91. Shi Z, et al. 2021. Ann Clin Transl Neurol. 8:43. PubMed
  92. Tang B, et al. 2020. Clin Cancer Res. 26:2216. PubMed
  93. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  94. Almshayakhchi R, et al. 2021. Front Oncol. 11:636977. PubMed
  95. Nguyen K, et al. 2008. J Immunol. 181:5386. PubMed
  96. Wilcox R, et al. 2009. Blood. 114:2936. PubMed
  97. Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed
  98. Griffiths K, et al. 2016. Nat Commun. 7:13894. PubMed
  99. Ciarlo E, et al. 2016. Sci Rep. 6:37944. PubMed
  100. Al-Attar A, et al. 2016. Data Brief. 8: 536-44. PubMed
  101. Gonzalez-Perez G, et al. 2016. J Immunol. 196: 3768 - 3779. PubMed
  102. Pablo-Bernal R, et al. 2015. J Infect Dis. 10.1093/infdis/jiv520. PubMed
  103. Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed
  104. Jeong J, et al. 2021. Immune Netw. 21:e31. PubMed
  105. Jondle CN, et al. 2021. MBio. 12:. PubMed
  106. Martens A, et al. 2020. Nat Immunol. 21:381. PubMed
  107. Wang Z, et al. 2020. Autophagy. 1:. PubMed
  108. Jiang S, et al. 2020. Scand J Immunol. e12867:91. PubMed
  109. Hradilova N, et al. 2017. PLoS One. 12(2):e0171539. PubMed
  110. Smithey M, et al. 2008. J Immunol. 180:3406. PubMed
  111. Rana A, et al. 2020. Front Immunol. 11:536. PubMed
  112. Dayoub O, et al. 2017. Sci Rep. 10.1038/s41598-017-09933-4. PubMed
  113. Kang X, et al. 2022. Vaccines (Basel). 10:. PubMed
  114. Doll K, et al. 2016. J Immunol. 196: 4253 - 4262. PubMed
  115. Liu W, et al. 2016. J Virol. 90: 6085 - 6096. PubMed
  116. Fucikova J, et al. 2016. Blood. 128(26):3113-3124. PubMed
  117. Douthwaite J, et al. 2017. J Immunol. 198(1):528-537. PubMed
  118. Yu H, et al. 2011. J Leukoc Biol. 90:205. PubMed
  119. Zhao F, et al. 2022. Front Immunol. 13:873720. PubMed
  120. Snyder LM, et al. 2022. Immunohorizons. 6:660. PubMed
  121. Rialdi A, et al. 2016. Science. 352: aad7993. PubMed
  122. Li H, et al. 2012. J Immunol. 189:5572. PubMed
  123. Liu W, et al. 2010. J Virol. 84:12011. PubMed
  124. Elzey B, et al. 2008. Blood. 111:3684. PubMed
  125. Ries M, et al. 2017. PLoS Pathog. 10.1371/journal.ppat.1006506. PubMed
  126. Satoh M, et al. 2016. Sci Rep. 6:28473. PubMed
  127. Codo AC, et al. 2020. Cell Metab. 32:437. PubMed
  128. Littwitz-Salomon E, et al. 2021. Nat Commun. 12:5376. PubMed
  129. Carissimo G, et al. 2019. Life Sci Alliance. 2:e201900298. PubMed
  130. Wang Y, et al. 2020. Sci Adv. 6:eaba5412. PubMed
  131. Uddin MN, et al. 2022. Brain Behav Immun Health. 26:100563. PubMed
  132. Shenoy AT, et al. 2021. Nat Commun. 12:5834. PubMed
  133. Ulrich-Lewis JT, et al. 2022. Front Immunol. 13:861710. PubMed
  134. Elliott PR, et al. 2021. Cell Rep. 37:109777. PubMed
  135. Dumaine JE, et al. 2021. Elife. 10:. PubMed
  136. Cheng B, et al. 2022. Cancer Commun (Lond). 42:17. PubMed
  137. Jondle CN, et al. 2021. J Virol. 95:e0072621. PubMed
  138. Bunting MD, et al. 2022. Sci Adv. 8:eabk3327. PubMed
  139. Gross C, et al. 2016. Neurol Neuroimmunol Neuroinflamm. 3: e289. PubMed
  140. Krämer B, et al. 2016. PLoS One. 11: 0162068. PubMed
  141. Tawara I, et al. 2011. Clin Cancer Res. 0.761805556. PubMed
  142. Orlov M, et al. 2017. PLoS One.. 10.1371/journal.pone.018296. PubMed
  143. Martins M, et al. 2017. PLoS Pathog. 10.1371/journal.ppat.1006529. PubMed
  144. Crooks SD, et al. 2022. Immunohorizons. 6:705. PubMed
  145. Fang E, et al. 2022. MedComm (2020). 3:e117. PubMed
  146. Lee T, et al. 2013. J Biol Chem. 288:32873. PubMed
  147. Vigano S, et al. 2015. J Virol. 89: 7829-7840. PubMed
  148. Vacaflores A, et al. 2016. PLoS One. 11: 0157175. PubMed
  149. Ma H, Wan S, Xia C 2016. J Leukoc Biol. 99: 1121 - 1129. PubMed
  150. Kim J, et al. 2016. Nat Immunol. 17:1159-1166. PubMed
  151. Johnson AM, et al. 2020. J Immunol. 204:2295. PubMed
  152. Buqué A, et al. 2020. Nat Commun. 3.110416667. PubMed
  153. Hassan AO, et al. 2020. Cell. 183:169. PubMed
  154. Harty N 2011. J Immunol. 186:3836. PubMed
  155. Iwata Y, et al. 2011. Blood. 117:530. PubMed
  156. Cao Y, et al. 2018. Nat Commun. 2.940972222. PubMed
  157. Emmer BT, et al. 2018. Elife. 7:e38839. PubMed
  158. Matisz C, et al. 2017. Sci Rep. 7:40631. PubMed
  159. Castro I, et al. 2016. J Virol. 90: 5280 - 5291. PubMed
  160. Zhang W, et al. 2015. Infect Immun . 83: 3213-3223. PubMed
  161. Yan H, et al. 2020. ACS Appl Mater Interfaces. 12:19324. PubMed
  162. Ji L, et al. 2012. PLoS One. 7:e50909. PubMed
  163. Redfern C, et al. 2006. J Clin Oncol. 24:3107. PubMed
  164. Huang RS, et al. 2021. Curr Protoc. 1:e246. PubMed
  165. Li G, et al. 2021. Curr Protoc. 1:e50. PubMed
  166. Gao Y, et al. 2022. Immunity. 55:1732. PubMed
  167. Han K, et al. 2021. Nat Metab. 3:318. PubMed
  168. Peng L, et al. 2022. Cell Rep Med. 3:100634. PubMed
  169. Bachy S, et al. 2022. iScience. 25:103758. PubMed
  170. Ohtsuka J, et al. 2021. iScience. Online ahead of print. PubMed
  171. Nice T, et al. 2016. PLoS One. 12: 1005684. PubMed
  172. Pfeifle R, et al. 2016. Nat Immunol. 18:104-113. PubMed
  173. Krishnan A, et al. 2020. Am J Pathol. 190:1284. PubMed
  174. Hiza H, et al. 2021. Sci Rep. 11:13190. PubMed
  175. Grace PS, et al. 2021. Front Immunol. 12:679973. PubMed
  176. Ngwenyama N, et al. 2019. JCI Insight. 4:e125527. PubMed
  177. Good Z, et al. 2019. Nat Biotechnol. 37:259. PubMed
  178. Butchar J, et al. 2010. Clin Cancer Res. 2.100694444. PubMed
  179. Wang J, et al. 2013. J Virol. 87:4372. PubMed
  180. Li Y, et al. 2021. Vaccines (Basel). 9:. PubMed
  181. Liu W, et al. 2022. Bio Protoc. 12:e4285. PubMed
  182. Ma K, et al. 2022. iScience. 25:104347. PubMed
  183. Zhang Y, et al. 2022. Vaccines (Basel). 10: . PubMed
  184. Matsushita T, et al. 2015. Rheumatology. 101093/rumatology/kv331. PubMed
  185. Stepanova E, et al. 2021. Microorganisms. 9:. PubMed
  186. Wang Y, et al. 2020. Virol Sin. 36:122. PubMed
  187. Loeuillard E, et al. 2020. J Clin Invest. 130:5380. PubMed
  188. Molinero L, et al. 2012. Proc Natl Acad Sci U S A. 109:18529. PubMed
  189. Kalafati L, et al. 2020. Cell. 183:771. PubMed
  190. Sekine T, et al. 2020. Cell. 183:158. PubMed
  191. Gogokhia L, et al. 2019. Cell Host Microbe. 25:285. PubMed
  192. Guo C, et al. 2018. Immunity. 49:842. PubMed
  193. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  194. Rossato C, et al. 2017. Biologicals. 10.1016/j.biologicals.2017.06.007. PubMed
  195. Tajima M, et al. 2022. Curr Protoc. 2:e540. PubMed
  196. de Picciotto S, et al. 2022. Nat Commun. 13:3866. PubMed
  197. Michelozzi IM, et al. 2022. STAR Protoc. 3:101174. PubMed
  198. Teh MR, et al. 2021. Front Immunol. 12:714613. PubMed
  199. Rozková D, et al. 2009. Clin Immunol. 131:1. PubMed
  200. Miner M, et al. 2008. Microb Pathog. 45:273. PubMed
  201. Miller M, et al. 2014. Proc Natl Acad Sci U S A. 111:7397. PubMed
  202. Gartlan K, et al. 2015. Blood. 126: 1609-1620. PubMed
  203. Ulland T, et al. 2016. Nat Commun. 7:13180. PubMed
  204. Melacarne A, et al. 2021. Cell Reports. 36(1):109312. PubMed
  205. Raju S, et al. 2020. Cell Reports. 29(9):2556-2564.e3.. PubMed
  206. Rosser EC, et al. 2020. Cell Metabolism. 31(4):837-851. PubMed
  207. Zenaro E, et al. 2009. J Leukoc Biol. 86:1393. PubMed
  208. Goodridge H, et al. 2009. J Immunol. 182:1146. PubMed
  209. Gnerlich J, et al. 2010. J Immunol. 185:4063. PubMed
  210. Boisvert M, et al. 2010. J Virol. 84:7782. PubMed
  211. Langkjær A, et al. 2012. Clin Immunol. 145:1. PubMed
  212. Tse SW, et al. 2021. Mol Ther. 29:2227. PubMed
  213. Cérbulo-Vázquez A, et al. 2022. PLoS One. 17:e0264566. PubMed
  214. Beyaz S, et al. 2016. Nat Immunol. 18:184-195. PubMed
  215. Kadivar M, et al. 2016. J Immunol. 197(12):4584-4592. PubMed
  216. Wang J, et al. 2013. J Virol. 87:11262. PubMed
  217. Virtakoivu R, et al. 2021. Clin Cancer Res. 27:4205. PubMed
  218. Koschel J, et al. 2021. Cell Death Differ. 28:2257. PubMed
  219. FitzPatrick MEB, et al. 2021. Cell Rep. 34:108661. PubMed
  220. Bass VL, et al. 2021. Mol Syst Biol. 17:e10127. PubMed
  221. Theisen U, et al. 2020. J Cell Biol. :219. PubMed
  222. Dáňová K, et al. 2017. J Immunol. 198(2):729-740. PubMed
  223. Markey K, et al. 2009. Blood. 113:5644. PubMed
  224. Salazar V, et al. 2019. Cell Rep. 26:1585. PubMed
  225. Zischke J, et al. 2017. PLoS Pathogens. 13(6):e1006454. PubMed
  226. Ma Q, et al. 2022. Vaccines (Basel). 10:. PubMed
  227. Rhoades NS, et al. 2022. Cell Rep. 39:110725. PubMed
  228. Reuschl AK, et al. 2022. Cell Rep. 39:110650. PubMed
  229. Deng Y, et al. 2021. Nat Commun. 12:7041. PubMed
  230. Li Z, et al. 2021. JCI Insight. 6:. PubMed
  231. Bulati M, et al. 2014. Exp Gerontol. 54:123. PubMed
  232. Zhou G, et al. 2014. J Cell Sci. 127:3916. PubMed
  233. Rist M, et al. 2015. J Virol. 89:703. PubMed
  234. Hoechst B, et al. 2016. J Immunol. 195:1517-1523. PubMed
  235. Festag J, et al. 2020. Mol Ther Nucleic Acids. 1.330555556. PubMed
  236. Mohammadpour H, et al. 2020. JCI Insight. 5:00. PubMed
  237. Fukuoka A, et al. 2013. Int Immunol. 25:373. PubMed
  238. Landi A, et al. 2017. Vaccine.. 10.1016/j.vaccine.2017.10.072. PubMed
  239. Gussarow D, et al. 2021. Front Med (Lausanne). 8:770381. PubMed
  240. Mackroth M, et al. 2016. PLoS Pathog. 12:e1005909. PubMed
  241. Rau M, et al. 2016. J Immunol. 196: 97 - 105. PubMed
  242. Genardi S, et al. 2020. Front Immunol. 11:610010. PubMed
  243. Wang F, et al. 2021. Neoplasia. 23:281. PubMed
  244. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
  245. Bonavita E, et al. 2020. Immunity. 1215:53. PubMed
  246. Gebremeskel S, et al. 2017. Cancer Immunol Res. 0.9625. PubMed
  247. Olafsdottir TA, et al. 2022. Commun Biol. 5:914. PubMed
  248. Lei Y, et al. 2021. Sci Adv. 7:. PubMed
  249. van Loon K, et al. 2022. Cancers (Basel). 14:. PubMed
  250. Krishna B, et al. 2016. Sci Rep. 6: 24674. PubMed
  251. Schridde A, et al. 2017. Mucosal Immunol. 10.1038/mi.2016.142. PubMed
  252. Wang W, et al. 2020. STAR Protoc. 1:100218. PubMed
  253. Ruland C, et al. 2017. Brain Behav Immun. 10.1016/j.bbi.2017.06.010. PubMed
  254. Ahmadi H,et al. 2017. Inflammopharmacology. . 10.1007/s10787-017-0410-8. PubMed
  255. Li J, et al. 2018. Redox Biol. 0.976388889. PubMed
  256. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  257. Abshire C, et al. 2016. PLoS Pathog. 12:e1006088. PubMed
  258. Ikebuchi R, et al. 2016. Sci Rep. 6:35002. PubMed
  259. Matei DE, et al. 2021. Med (N Y). 2:864. PubMed
  260. Laczkó D, et al. 2020. Immunity. 53:724. PubMed
  261. Lu G, et al. 2017. PLoS One. 12:e0178352. PubMed
  262. Orozco SL, et al. 2019. Cell Rep. 28:2275. PubMed
  263. Ying B, et al. 2022. Sci Transl Med. 14:eabm3302. PubMed
  264. Guo Z, et al. 2022. Neurobiol Pain. 12:100096. PubMed
  265. Carrion B, et al. 2021. Neurol Neuroimmunol Neuroinflamm. 8:. PubMed
  266. Petrova T, et al. 2021. FEBS J. 288:5909. PubMed
  267. Nesterenko PA, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed

Antigen Details

Antigen References

1. Current Protocols in Immunology (John Wiley & Sons New York) Unit 6.24 Detection of Intracellular Cytokines by Flow Cytometry (Barbara Foster and Calman Prussin NIAID NIH Bethesda MD).
2. Sander B, et al. 1991. Immunol. Rev. 119:65.
3. Sander B, et al. 1993. J. Immunol. Meth. 166:201.
4. Prussin C, et al. 1995. J. Immunol. Meth. 188:117.
5. Jung T, et al. 1993. J. Immunol. Meth. 159:197.

Gene ID
NA

Related FAQs

The Brefeldin A solution freezes at 4°C . Is the solution still OK?

We recommend to thaw in warm water after removing from the fridge.  The Brefeldin A is dissolved in DMSO, which freezes at 18°C, so this is normal.  No one has reported any problems with the freeze/thaw cycles and this is how we recommend it is stored.   If customers are concerned, they can aliquot the Brefeldin A to avoid the freeze thaw.

How do I choose monensin or Brefeldin A solution?

Generally, Brefeldin A is more toxic for longer term incubations, so for shorter stimulations (6 hrs or less) use Brefeldin A.  For longer stimulations use monensin.  We recommend optimization for each cell type and protocol.

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For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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