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Cell-Vive™ GMP NKp46/CD2 Human NK Cell Activation Beads

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1_CV_BEADS_NKp46-CD2_Human_NK_Cell_Activation_042225
Human peripheral blood mononuclear cells were stimulated with Cell-Vive™ GMP NKp46/CD2 Human NK Cell Activation Beads (magenta for all figures) or a competitor’s equivalent product (teal for all figures) at a bead-to-cell ratio of 1:2 for 14 days. Cells were cultured in IMDM containing 5% human AB serum, 100 IU/mL of recombinant IL-2 (cat# 791902) and 10 ng/mL of recombinant IL-15 (cat# 570314). Cells were split on D6 and D9 of culture and re-seeded at a density of 1.0 x 106 cells per mL. Cells were harvested at D14 for total cell expansion and phenotype analysis. (A) Fold expansion of CD56+ cells was calculated by comparing the final percentage (determined by flow cytometry) of CD56+ cells and total cell counts on D14 with the starting percentage of CD56+ cells in the D0 PBMCs. (B) Total cell viability at D14. (C) Further phenotypic analysis was performed on the CD56+ cells at D14. Cells generated using Cell-Vive™ GMP NKp46/CD2 Human NK Cell Beads are plotted in magenta. Cells generated with a competitor’s equivalent product are plotted in teal.
  • 1_CV_BEADS_NKp46-CD2_Human_NK_Cell_Activation_042225
    Human peripheral blood mononuclear cells were stimulated with Cell-Vive™ GMP NKp46/CD2 Human NK Cell Activation Beads (magenta for all figures) or a competitor’s equivalent product (teal for all figures) at a bead-to-cell ratio of 1:2 for 14 days. Cells were cultured in IMDM containing 5% human AB serum, 100 IU/mL of recombinant IL-2 (cat# 791902) and 10 ng/mL of recombinant IL-15 (cat# 570314). Cells were split on D6 and D9 of culture and re-seeded at a density of 1.0 x 106 cells per mL. Cells were harvested at D14 for total cell expansion and phenotype analysis. (A) Fold expansion of CD56+ cells was calculated by comparing the final percentage (determined by flow cytometry) of CD56+ cells and total cell counts on D14 with the starting percentage of CD56+ cells in the D0 PBMCs. (B) Total cell viability at D14. (C) Further phenotypic analysis was performed on the CD56+ cells at D14. Cells generated using Cell-Vive™ GMP NKp46/CD2 Human NK Cell Beads are plotted in magenta. Cells generated with a competitor’s equivalent product are plotted in teal.
  • 2_CV_BEADS_NKp46-CD2_Human_NK_Cell_Activation_042225
  • 3_CV_BEADS_NKp46-CD2_Human_NK_Cell_Activation_061225
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422611 0.5 mL 475 CHF
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422612 2.5 mL 1575 CHF
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Description

Cell-Vive™ GMP NKp46/CD2 Human NK Cell Activation Beads allow for convenient in vitro or ex vivo activation of human NK cells without the need for antigen-presenting cells or feeder cells. Human NK cells are stimulated with anti-NKp46 and anti-CD2 antibodies coated on superparamagnetic microbeads. Simply add the beads directly to isolated NK cells or to PBMCs at a 1:2 bead-to-cell ratio for robust NK cell activation. Excess antibody-coated beads can be removed from the cell suspension using a magnetic separator (some residual beads may be retained in the cell suspension). Some of the downstream applications include functional assays, gene expression analysis, and phenotypic characterization.

Product Details
Quality Statement

BioLegend Cell-Vive™ GMP Activation and Expansion Tools are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue Engineered Products and Ph. Eur. Chapter 5.2.12 in a dedicated GMP facility compliant with ISO 13485:2016. Specifications and processes include

  • Low endotoxin level (< 1 EU/mL)
  • Manufactured under serum-free conditions
  • Bioburden/sterility and mycoplasma testing
  • Batch-to-batch consistency
  • Vendor qualification
  • Raw material traceability and documentation
  • Documented procedures and employee training
  • Equipment maintenance and monitoring records
  • Lot-specific certificates of analysis
  • Quality audits per ISO 13485:2016
  • QA review of released products
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Formulation
Beads are resuspended in phosphate buffer solution containing 0.1% recombinant HSA.
Endotoxin Level
< 1 EU/mL
Concentration
4.0 x 107 ± 10 % beads/mL
Storage & Handling
4°C (2°C-8°C)
Application

Cell Culture - Quality tested

Activation - Verified in house
Application Notes

This product is designed for the in vitro or ex vivo expansion of human NK cells.

 

Cell-Vive™ GMP NKp46/CD2 Human NK Cell Activation Beads are a ready to use solution. There is no need to dilute the product prior to use in cell culture.

 

Suggested culture protocol:

  1. Prepare NK Cell Complete Media (NKCCM) using sterile, filtered IMDM with 5% human AB serum or desired serum subsititute, 100 IU/mL of human IL-2, and 10 ng/mL of human IL-15.
  2. Prepare cells at a concentration of 1 x 106 per mL of NKCCM.
  3. Wash 12.5 µL of Cell-Vive™ GMP NKp46/CD2 Human NK Cell Activation Beads to a 5 mL polypropylene tube with 1 mL NKCCM.
  4. Apply tube containing beads to a magnet (MojoSort magnet, cat#: 480019) for 2 minutes. Decant supernatant and remove tube from magnet.
  5. Resuspend beads in 1 mL of cell suspension and plate in a 12 well cell culture plate.
  6. Allow cells to expand at 37°C and 5% CO2. At D6, D9, and D14, count cells and re-seed at 1.0 x 106 cells per mL.
  7. At D14, harvest cells for further analysis or desired applications.

This product was optimized on isolated CD56+ NK cells and PBMCs. Alternate sources of NK cells may require protocol optimization.

Disclaimer

BioLegend Cell-Vive™ GMP Activation and Expansion tools are for research use only. Suitable for ex vivo cell processing. Not for use in diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

Antigen Details

Gene ID
NA

Related FAQs

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Go To Top Version: 2    Revision Date: 10.29.2025

For Research Use Only. Suitable for ex vivo cell processing use. Not for injection or diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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