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- Clone
- S16016B.Rec (See other available formats)
- Other Names
- Prominin-1, PROM1, AC133
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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NCCIT cells were stained with Cell-Vive™ GMP Ultra-LEAF™ Biotin anti-human CD133 RecAb SF at 0.125 µg per test (Clone S16016B.Rec, cat#: 394021, filled histogram) or an isotype control (Clone MOPC-173, cat#: 400203, open histogram). Both primary stains were followed by PE Streptavidin (cat#: 405203).
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 394022 | 1 mg | 2525 CHF | ||||
| 394021 | 100 µg | 492 CHF | ||||
CD133, also known as Prominin-1 and AC133 antigen, is a 120 kD pentaspan glycoprotein with 5 transmembrane domains. CD133 was initially described as a surface antigen specific for human hematopoietic stem cells and as a marker for murine neuroepithelial cells and some embryonic epithelia. Later on, CD133 was found on other stem cells, including endothelial progenitor cells, glioblastomas, neuronal, and glial stem cells. In addition to stem cells for normal tissue, CD133 was found on cancer cells, such as some leukemia cells and brain tumor cells. Although the biological function of CD133 is not completely understood, CD133 has been extensively used as a stem cell marker for normal and cancerous tissues.
Product DetailsBioLegend Cell-Vive™ GMP Ultra-LEAF™ antibodies are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12 in a dedicated GMP facility compliant with ISO 13485:2016. Specifications and processes include
- Ultra-Low endotoxin level (< 0.01 EU/µg)
- Manufactured under serum-free conditions
- Bulks tested negative for mycoplasma and bacterial/fungal growth
- Serum-free hybridomas are negative for the presence of murine virus
- Batch-to-batch consistency
- Vendor qualification
- Raw material traceability and documentation
- Documented procedures and employee training
- Equipment maintenance and monitoring records
- Lot-specific certificates of analysis
- QA review of released products
- Quality audits per ISO 13485:2016
Product Details
- Reactivity
- Human
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Formulation
- 0.1 µm filtered in phosphate-buffered solution, pH 7.2, with a final concentration of HPCD at 5%. Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Endotoxin Level
- Less than 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
- Preparation
- GMP Ultra-LEAF™ (Low Endotoxin, Azide-Free) Recombinant Serum Free antibody purified by affinity chromatography
- Concentration
- 1 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Disclaimer
-
BioLegend Cell-Vive™ GMP Ultra-LEAF™ antibodies are for research use only. Suitable for ex vivo cell processing use. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.
Antigen Details
- Structure
- 120 kD pentaspan transmembrance glycoprotein with 5 transmembrane domains.
- Distribution
-
Hematopoietic stem cells and progenitor cells, fetal liver cells, tissue specific stem cells or progenitor cells such as renal and prostate, and a variety of tumor cells.
- Function
- May play a role in cell differentiation, proliferation, and apoptosis.
- Interaction
- NAT8; NAT8B; CDHR1
- Antigen References
-
- Yin AH, et al. 1997. Blood. 90:5002.
- Miraglia S, et al. 1997. Blood. 90:5013.
- Bühring HJ, et al. 1999. Ann. NY Acad. Sci. 872:25.
- Gene ID
- 8842 View all products for this Gene ID
- UniProt
- View information about CD133 on UniProt.org
Related FAQs
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Other Formats
View All CD133 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Cell-Vive™ GMP Ultra-LEAF™ Biotin anti-HU CD133 RecAb SF | S16016B.Rec | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Cell-Vive™ GMP Ultra-LEAF™ Biotin anti-HU CD133 RecAb SF
NCCIT cells were stained with Cell-Vive™ GMP Ultra-LEAF™ Bio...
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