Loading...

FluoroSpot MAX™ Deluxe Set Mouse TNF-α

Pricing & Availability
Regulatory Status
RUO
Other Names
Tumor Necrosis Factor alpha, Cachectin, Tumor necrosis factor ligand superfamily member 2
Ave. Rating
Submit a Review
Product Citations
publications
Fluorospot_Mouse-TNF-a_100725
Mouse Splenocytes (75,000 cells / well) were incubated for 20 hours in the absence or presence of 100 ng/mL LPS. The number of cells secreting Mouse TNF-αwas analyzed by FluoroSpot and quantified using an image cytometer equipped with the required fluorescence filter.
  • Fluorospot_Mouse-TNF-a_100725
    Mouse Splenocytes (75,000 cells / well) were incubated for 20 hours in the absence or presence of 100 ng/mL LPS. The number of cells secreting Mouse TNF-αwas analyzed by FluoroSpot and quantified using an image cytometer equipped with the required fluorescence filter.
Cat # Size Price Quantity Check Availability Save
430926 5 plates 770 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

TNF-α (Tumor Necrosis Factor α), also known as Cachectin, is a cytokine belonging to the Tumor Necrosis Factor superfamily. It is produced by various cell types, including macrophages, monocytes, neutrophils, T cells, NK cells, and epithelial cells. TNF-α can bind to two receptors: TNFR1 and TNFR2. TNF-α signaling plays a central role in the inflammatory response, lymphoid cell development, and lipid metabolism. Additionally, TNF-α is a pathological component in numerous autoimmune diseases, including but not limited to Crohn’s disease, asthma, and rheumatoid arthritis. It was also reported to be involved in some cancers and obesity.

BioLegend’s FluoroSpot MAX™ Deluxe Set contains pre-optimized essential reagents, buffers, and solutions for the detection of Mouse TNF- α secreting cells. In this assay, anti-mouse TNF- α capture antibodies are coated on a clear-bottom microplate. Cells (stimulated or unstimulated) are added to the wells and incubated, allowing secreted TNF- α to bind to nearby capture antibodies. After incubation, cells are removed, and biotinylated detection antibodies are added followed by Streptavidin-PE. The resulting red spots represent the “footprint” of an individual TNF- α-secreting cell, allowing for precise quantification of cytokine-producing cells in the sample.

Product Details
Technical Data Sheet (pdf)

Kit Details

Kit Contents
  • Mouse TNF- α Capture Antibody (200X) 
  • Mouse TNF- α Detection Antibody (200X) 
  • Mouse TNF- α Standard (used as positive control) 
  • FluoroSpot SA-PE (1000X) 
  • Coating Buffer A (5X) 
  • Assay Diluent B (5X)
Materials Not Included
  • Corning® Clear Bottom Black Polystyrene Microplates, 96 Well (Catalog No. 3601) is recommended, or equivalent black walled, clear bottom microplate is suggested.
  • Black Microplate Lid: Corning® Universal Black Microplate Lid with Corner Notch (Catalog No. 3935) is recommended, or equivalent
  • Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20 
  • Plate Sealers: BioLegend Cat. No. 423601 is recommended
  • 0.22 μm filters
  • Sterile PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.22 μm filtered
  • Sterile cell culture media
  • 37oC CO2 incubator
  • Deionized (DI) water
  • Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL
  • Wash bottle, manifold dispenser, or automated microplate washer
  • Tubes for dilution
  • Timer
  • Alcohol Wipes
  • Image cytometer or other imaging platform capable of whole-well imaging. The device should be equipped with the appropriate filters for the fluorophores utilized in each assay.

Product Details

Verified Reactivity
Mouse
Application

FluoroSpot

Antigen Details

Cell Type
Antigen-presenting cells, B cells, Granulocytes, Langerhans cells, Leukocytes, Lymphocytes, Macrophages, Mast cells, Monocytes, Neutrophils, NK cells, NKT cells, Plasma cells, T cells, Tfh, Th1, Th17, Th2, Thymocytes, Tregs
Biology Area
Adaptive Immunity, Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Death, Immuno-Oncology, Immunology, Inhibitory Molecules, Innate Immunity, Mitochondrial Function, Signal Transduction, Ubiquitin/Protein Degradation
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, Cytokines/Chemokines, GTPases, Phospho-Proteins, Proteases, Protein Kinases/Phosphatase, Soluble Receptors, Toll Like Receptors, Tumor Suppressors
Gene ID
21926 View all products for this Gene ID
UniProt
View information about TNF-alpha on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10.07.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account