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Purified anti-Annexin A2

Pricing & Availability
Clone
W19153A (See other available formats)
Regulatory Status
RUO
Other Names
Annexin II, Annexin-2, ANXA2, Calpactin I heavy chain, Calpactin-1 heavy chain
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A. W19153A_PURE_Annexin-A2_WB_082125
Whole cell extracts (15 µg total protein per lane) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-Annexin A2 (clone W19153A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A. W19153A_PURE_Annexin-A2_WB_082125
    Whole cell extracts (15 µg total protein per lane) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-Annexin A2 (clone W19153A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • B. W19153A_PURE_Annexin-A2_IHC-P_082125
    IHC staining with Purified anti-Annexin A2 (clone W19153A), on formalin-fixed paraffin-embedded human esophagus tissue. Following antigen retrieval using 1X Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704), the tissue was incubated without (panel A) or with (panel B) Purified anti-Annexin A2 (clone W19153A), followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective and merged (panels C and D). Scale bar: 50 µm
  • C. W19153A_PURE_Annexin-A2_ICC_082125
    HEK293 cells (low-expressing cell line, panels A and C) and HaCaT cells (high-expressing cell line, panels B and D) were fixed and permeabilized with 100% ice-cold methanol for 10 minutes and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with Purified anti-Annexin A2 (clone W19153A), followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective and merged (panels C and D). Scale bar: 50 µm
  • D. W19153A_PURE_Annexin-A2_ICFC_082125
    K-562 cells (high-expressing cell line, filled histogram) and HL-60 cells (low-expressing cell line, open histogram) were fixed and permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401) and intracellularly stained with Purified anti-Annexin A2 (clone W19153A), or Purified Rat IgG2a, κ Isotype Control (dashed histogram, representative for both cell lines) (Cat. No. 400502) followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416).
Cat # Size Price Quantity Check Availability Save
648838 25 µg 170 CHF
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648839 100 µg 410 CHF
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Description

Annexin A2 (ANXA2), a member of the annexin family of calcium-dependent phospholipid-binding proteins. Annexin A2 contains a conserved core domain with four annexin repeats that facilitate calcium-dependent binding to negatively charged phospholipids. This protein plays roles in diverse cellular processes, including membrane trafficking, cytoskeletal rearrangement, fibrinolysis, inflammation, and cellular signal transduction. Annexin A2 contributes to the regulation of membrane dynamics and endocytosis by binding to negatively charged phospholipids in a calcium-dependent manner, facilitating vesicle aggregation, fusion, and cytoskeletal anchoring. It also supports actin filament organization, thereby influencing cellular shape, motility, and adhesion. Annexin A2 is involved in exocytosis and plays an essential role in the trafficking of proteins to and from the cell membrane. ANXA2 typically exists as a monomer or as a heterotetrameric complex with S100A10 (also known as p11), forming the Annexin A2/S100A10 heterotetramer (A2t). This complex is crucial for extracellular and membrane-associated functions. The A2t complex acts as a co-receptor for tissue plasminogen activator (tPA) and plasminogen on endothelial cell surfaces, promoting the localized generation of plasmin, a key enzyme involved in clot breakdown and extracellular matrix remodeling. 

Dysregulation of Annexin A2 expression or localization has been associated with various diseases. In cancer, Annexin A2 is frequently overexpressed and promotes tumorigenesis by enhancing cell proliferation, invasion, angiogenesis, and metastasis. It has been identified as a marker of poor prognosis in cancers such as breast, pancreatic, and lung cancer. Annexin A2 facilitates cancer cell migration through actin remodeling and matrix degradation, and its cell surface expression supports plasmin-mediated extracellular matrix breakdown, aiding invasion.

 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant full length human Annexin A2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, ICC, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry, a concentration range of 2.5 - 10.0 µg/mL is suggested. For immunohistochemistry, a concentration range of 1.25 - 10.0 µg/mL is suggested. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with either of the following:
-Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol
-100% ice-cold methanol only

For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either of the following:
-Citrate Buffer, 10X (Cat. No. 420902)
-Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704).

For use in intracellular flow cytometry (ICFC), it is recommended to fix/permeabilize with True-Phos™ Perm Buffer (Cat. No. 425401). 
It is not recommended to perform fixation/permeabilization with either of the following:
- Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803)
-True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401)

Antigen Details

Structure
Annexin A2 is a 339 amino acid length protein with a predicted molecular weight of 38.6 kD.
Distribution

Cytoplasm, cell surface, and extracellular space

Interaction
S100A10
Cell Type
Endothelial cells, Langerhans cells, Macrophages, Monocytes
Biology Area
Adaptive Immunity, Apoptosis/Tumor Suppressors/Cell Death, Cell Motility/Cytoskeleton/Structure, Cell Proliferation and Viability, Innate Immunity, Signal Transduction
Antigen References
  1. Pomay-Penabad Z, et al. 2009. Blood. 114(14):3074-83.
  2. Zhai H, et al. 2011. J. Neurosci. 31(40):14346-60.
  3. Lokman NA, et al. 2011. Cancer Microenviron. 4(2):199-208.
  4. Christensen MV, et al. 2018. Int. J. Oncol. 52(1):5-18.
Gene ID
302 View all products for this Gene ID
UniProt
View information about Annexin A2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08.21.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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