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Purified anti-ATP5A1 Antibody

Pricing & Availability
Clone
W22077A (See other available formats)
Regulatory Status
RUO
Other Names
ATP5F1A, alpha subunit ATP synthase isoform precursor (EC 3.6.1.34), ATP synthase alpha chain, mitochondrial, ATP synthase alpha subunit, ATP synthase alpha subunit precursor, ATP synthase F1 subunit alpha, ATP synthase subunit alpha
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
A. W22077A_PURE_ATP5A1_WB_61925
Whole cell extracts (15 µg total protein per lane) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with Purified anti-ATP5A1 (clone W22077A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:5000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A. W22077A_PURE_ATP5A1_WB_61925
    Whole cell extracts (15 µg total protein per lane) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with Purified anti-ATP5A1 (clone W22077A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:5000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • B. W22077A_PURE_ATP5A1_ICC-1_61925
    HeLa cells were fixed with ice-cold methanol and then blocked with 5% FBS for 1 hour at room temperature. Cells were then intracellularly stained with Purified Rat IgG2b, κ Isotype Ctrl Antibody (Cat. No. 400602) (panel A) or stained with Purified rat anti-ATP5A1 (clone W22077A) (panel C). Cells were also co-stained with Purified mouse anti-COX IV (Cat. No. 937802) (panel B). Secondary staining was done by incubation with Alexa Fluor® 488 Goat anti-mouse IgG (Cat. No. 405319) and Alexa Fluor® 594 Goat anti-rat IgG (Cat. No. 405422) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801) (blue, panels A and D). The image was captured with a 40X objective. Scale bar: 50 µm
  • C. W22077A_PURE_ATP5A1_ICC-2_61925
    HeLa cells with mock knockdown (panels A-C) or shRNA transient knockdown (panels D-F) were fixed with fixation buffer (Cat. No. 420801), permeabilized with ice-cold methanol, then blocked with 5% FBS for 1 hour at room temperature. Cells were then intracellularly stained with isotype control (Cat. No. 400602) (panels A and D) or stained with anti-ATP5A1 (clone W22077A), followed by incubation with Alexa Fluor 594 Goat anti-rat IgG (Cat. No. 405422) for 1 hour at room temperature. Then cells were co-stained with Alexa Fluor® 488 anti-GFP Antibody (Cat. No. 338008) (green) for 1 hour at room temperature to detect the knockdown reporter in transfected cells (panel F). Nuclei were counterstained with DAPI (Cat. No. 422801) (blue, merged in panels C and F). The image was captured with a 40X objective. Scale bar: 50 µm
  • D. W22077A_PURE_ATP5A1_IHC-P-1_61925
    IHC staining with Purified anti-ATP5A1 (clone W22077A) was performed on FFPE human liver tissue following antigen retrieval using 1X Citrate buffer (Cat. No. 420902). The tissue sections were incubated with 5 µg/mL of Purified anti-ATP5A1 (clone W22077A) (panels B and C) followed by Alexa Fluor® 594 Goat anti-rat (Cat. No. 405422) (red) or secondary antibody only (panel A). Nuclei were counterstained with DAPI (Cat. No. 422801) (blue) (panel A and merged in panel C). The image was captured wit a 40X objective. Scale Bar: 50 µm
  • E. product_images/W22077A_PURE_ATP5A1_IHC-P-2_61925
    IHC staining with Purified anti-ATP5A1 (clone W22077A) was performed on FFPE human heart tissue following antigen retrieval using 1X Citrate buffer (pH 6.0) (Cat. No. 420902). The tissue sections were incubated without (panel A) or with Purified anti-ATP5A1 (clone W22077A) (panels B and C) followed by Alexa Fluor® 594 Goat anti-rat (red, Cat. No. 405422). Nuclei were counterstained with DAPI (Cat. No. 422801) (blue, panels A and merged in panel C). The image was captured with a 40X objective. Scale Bar: 50 µm
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648951 25 µg 170 CHF
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648952 100 µg 430 CHF
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Description

ATP synthase F1 subunit alpha, also known as ATP5A1 or ATP5F1A, is a subunit of the mitochondrial complex V or F1F0 ATP synthase, which produces ATP from ADP. The enzyme ATP synthase has two complexes: F1, the soluble catalytic core, and Fo, which is composed of the proton channel. ATP5A1 is one of five different subunits that make up the main F1 core. Recent studies indicate ATP5A1 mutations lead to mitochondrial complex V deficiency which is a mitochondrial disorder with various symptoms such as dysmorphic features, psychomotor delays, hypotonia, growth impairment, cardiomyopathy, hepatomegaly, and hypoplastic renal structures. Furthermore, ATP5A1 is also associated with neurodevelopmental diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human ATP5A1 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.03125 - 0.25 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

This antibody has been tested in knockout/knockdown systems for ICC.

The antibody (clone W22077A) can cross-react with mouse in western blot (WB).

For immunocytochemistry (ICC), we recommend fixation with 4% PFA followed by permeabilization with ice cold-methanol, or ice-cold methanol fixation.

For immunohistochemistry on FFPE tissues (IHC-P), antigen retrieval with Citrate Buffer (Cat. No. 420901) or Tris-EDTA pH 9.0 (Cat. No. 422704) are recommended.

Antigen Details

Structure
ATP5A1 is a 553 amino acid protein with a predicted molecular weight of 60 kD.
Distribution

Mitochondrion inner membrane

Interaction
Interacts with ATPAF2, HRG, PLG, BLOC1S1, BCL2L1 isoform BCL-X(L), CLN5, PPT1, S100A1, and ABCB7
Cell Type
Neurons
Biology Area
Cell Biology, Mitochondrial Function, Neurodegeneration, Neuroscience
Molecular Family
Enzymes and Regulators, Mitochondrial Markers
Antigen References
  1. Kataoka H, et al. 1991. Biochim Biophys Acta. 1089:393.
  2. Jonckheere A.I, et al. 2013. Brain. 136:1544.
  3. M.A L, et al. 2021. European Journal of Human Genetics.29:1719.
  4. Zech M, et al. 2022. Ann Neurol. 91:225.
Gene ID
498 View all products for this Gene ID
UniProt
View information about ATP5A1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06.19.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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