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Purified anti-IRAK3 (IRAK-M) Antibody

Pricing & Availability
Clone
W23096A (See other available formats)
Regulatory Status
RUO
Other Names
IRAK-3, Interleukin-1 receptor-associated kinase 3, IL-1 receptor-associated kinase M, IRAKM, Inactive IL-1 receptor-associated kinase 3, ASRT5
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
A. W23096A_PURE_IRAK3_WB_050525
Whole cell extracts (15 µg total protein per lane) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-IRAK3 (IRAK-M) (clone W23096A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A. W23096A_PURE_IRAK3_WB_050525
    Whole cell extracts (15 µg total protein per lane) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-IRAK3 (IRAK-M) (clone W23096A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • B. W23096A_PURE_IRAK3_ICFC_050525
    THP-1 cells (positive cell line, filled histogram) and K-562 cells (negative cell line, open histogram) were fixed with Fixation Buffer (Cat. No. 420801), permeabilized with Intracellular Staining Permeabilization Wash Buffer (Cat. No. 421002) and intracellularly stained with Purified anti-IRAK3 (IRAK-M) (clone W23096A), or Purified Rat IgG2b, κ Isotype Control (open histogram, dashed line, representative of both the positive and negative cell lines) (Cat. No. 400602) followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416).
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646251 25 µg 170 CHF
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646252 100 µg 465 CHF
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Description

The interleukin-1 receptor-associated kinase (IRAK) family of proteins are made up of serine/threonine-specific kinases which play a critical role in the response to pathogens through the induction of acute inflammation and subsequent adaptive immune responses. The mammalian IRAK molecular family consists of four members (IRAK1, IRAK2, IRAK3/IRAK-M, and IRAK4). Two are active kinases, IRAK1 and IRAK4, and two are inactive kinases, IRAK2 and IRAK3. However, all are involved in the regulation of the nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) pathways. The binding of IL-1 to IL-1 receptor type I (IL-1RI) initiates the formation of a complex that includes IL-1R1, IL-1RAcP, MyD88, and IRAKs. Unlike its family members IRAK1 and IRAK4, which promote inflammatory responses, IRAK3 (IRAK-M) functions as a negative regulator, helping to maintain immune homeostasis and prevent excessive inflammation. It is predominantly expressed in monocytes, macrophages, and dendritic cells, where it plays an essential role in dampening TLR-mediated immune activation. 
IRAK3 prevents the dissociation of IRAK1 and IRAK4 from MyD88, thereby disrupting downstream activation of TRAF6. It also inhibits the phosphorylation and degradation of IRAK1, which is a crucial step in propagating TLR signaling. Moreover, IRAK3 suppresses NF-κB activation, reducing the transcription of inflammatory cytokines. This regulatory role is essential for preventing excessive inflammation and avoiding conditions such as sepsis and chronic inflammatory diseases. Loss of IRAK3 function has been associated with hyperinflammatory states, leading to conditions such as sepsis, acute lung injury, and inflammatory bowel disease. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human IRAK3 (IRAK-M)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICFC - Verifed
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

For use in intracellular flow cytometry (ICFC), it is recommended to fix with Fixation Buffer (Cat. No. 420801) and permeabilize with either of the following:
-Intracellular Staining Permeabilization Wash Buffer (Cat. No. 421002)
-True-Phos™ Perm Buffer (Cat. No. 425401)
-True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401)

RRID
AB_3699073 (BioLegend Cat. No. 646251)
AB_3699073 (BioLegend Cat. No. 646252)

Antigen Details

Structure
IRAK3 is a 596 amino acid length protein with a predicted molecular weight of 67.8 kD and has a 60.6 kD isoform.
Distribution

Cytoplasm

Cell Type
Dendritic cells, Eosinophils, Macrophages, Monocytes
Biology Area
Immuno-Oncology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
Protein Kinases/Phosphatase
Antigen References
  1. Kobayashi K, et al. 2002. Cell. 110:191-202.
  2. Miyata M, et al. 2015. Nat Commun. 2015. 14:6:6062.
  3. Zhang C-J, et al. 2018. J Clin. Invest. 2018. 128:5399-5412.
  4. Liu W, et al. 2022. Nat Commun. 2022. 13:2748.
Gene ID
11213 View all products for this Gene ID
UniProt
View information about IRAK3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05.05.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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