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Purified anti-LDHA Antibody

Pricing & Availability
Clone
A22115F (See other available formats)
Regulatory Status
RUO
Other Names
L-lactate dehydrogenase A chain (LDHA), cell proliferation inducing gene 19, LDH muscle subunit (LDH-M), Renal carcinoma antigen NY-REN-59, PIG19
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A. A22115F_PURE_LDHA_WB_052925
Whole cell extracts (15 µg total protein per lane) from HAP1 cells with or without LDHA CRISPR knockout were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-LDHA (clone A22115F) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG Antibody (Cat. No. 405306). Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:10000 dilution. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. The knockout cell line was acquired from Horizon Discover (Human HAP1 Knockout Cell Line – LDHA, Horizon Cat. No. HZGHC004917c008). Lane M: Molecular weight marker
  • A. A22115F_PURE_LDHA_WB_052925
    Whole cell extracts (15 µg total protein per lane) from HAP1 cells with or without LDHA CRISPR knockout were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-LDHA (clone A22115F) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG Antibody (Cat. No. 405306). Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:10000 dilution. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. The knockout cell line was acquired from Horizon Discover (Human HAP1 Knockout Cell Line – LDHA, Horizon Cat. No. HZGHC004917c008). Lane M: Molecular weight marker
  • B. A22115F_PURE_LDHA_WB-2_052925
    Whole cell extracts (15 µg total protein per lane) from indicated cell lines or recombinant proteins were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-LDHA (clone A22115F) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG Antibody (Cat. No. 405306). Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:10000 dilution. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker
  • C. A22115F_PURE_LDHA_IHC-P_052925
    IHC staining with Purified anti-LDHA (clone A22115F) on formalin-fixed paraffin-embedded human liver tissue. Following antigen retrieval using 1X Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704), the tissue was incubated without (panel A) and with (panel B) Purified anti-LDHA (clone A22115F) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective and merged.
  • d. A22115F_PURE_LDHA_ICC_052925
    HAP1 cells (panel A) and HAP1 LDHA knockout cells (Horizon Cat. No. HZGHC004917c008, panel B) were fixed with 4% PFA Fixation Buffer (Cat. No. 420801) for 10 minutes and permeabilized with 100% ice-cold methanol for 10 minutes and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with Purified anti-LDHA (clone A22115F), followed by incubation with Alexa Fluor® 594 Goat anti-mouse IgG (red, Cat. No. 405326) for 1 hour at room temperature. Nuclei were counterstained with DAPI (blue, Cat. No. 422801). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective and merged. Scale bar: 50 µm
  • e. A22115F_PURE_LDHA_ICFC_052925
    HAP1 cells (positive cell line, filled histogram) and HAP1 LDHA knockout cells (negative cell line, open histogram) (Horizon Cat. No. HZGHC004917c008) were fixed and permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401) and intracellularly stained with Purified anti-LDHA (clone A22115F), or Purified Mouse IgG2b, κ Isotype Control (clone MG2b-57, Cat. No. 401201) (open histogram, dashed line, representative of both the positive and negative cell lines) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322).
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648151 25 µg 165 CHF
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648152 100 µg 430 CHF
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Description

L-lactate dehydrogenase A chain, also known as LDHA, is an enzyme that plays a key role in cellular energy metabolism, particularly under anaerobic conditions. LDHA catalyzes the conversion of pyruvate to lactate, which coupled with the oxidation of NADH to NAD+ is essential for maintaining glycolysis when oxygen is limited. LDHA is widely expressed, with the highest levels in tissues with high energy demands such as muscle. In cancer cells, LDHA supports the Warburg effect by promoting lactate production, thus LDHA is a potential target for cancer therapy due to its role in tumor progression and metabolic reprogramming.

Product Details
Quality Statement

This product was carefully developed for performance and specificity using the following product from Horizon Discovery, a Revvity company: Human HAP1 Knockout Cell Line – LDHA (Horizon Cat. No. HZGHC004917c008)

Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human LDHA
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, ICC, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704).

For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with any of the following:
- Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol
- 100% ice-cold methanol only
Note: Fixation/ permeabilization with Fixation buffer (Cat. No. 420801) followed by 0.5 % Triton X-100 is not recommended due to dim signal.

For use in intracellular flow cytometry (ICFC), it is recommended to fix/permeabilize with Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) or True-Phos™ Perm Buffer (Cat. No. 425401). Signal is brighter using True-Phos™ Perm Buffer. 
Note: Fixation/permeabilization with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) is not recommended due to dim signal.

RRID
AB_3699075 (BioLegend Cat. No. 648151)
AB_3699075 (BioLegend Cat. No. 648152)

Antigen Details

Structure
LDHA is a 332 amino acid protein with a predicted molecular weight of 36.7 kD.
Distribution

Cytoplasm

Interaction
HSP90: Helps stabilize and fold LDHA, particularly under stress conditions
GAPDH: LDHA forms complexes with other glycolytic enzymes to facilitate metabolism
AKT: AKT phosphorylates LDHA, influencing its activity
Biology Area
Cancer Biomarkers, Cell Biology
Molecular Family
Enzymes and Regulators
Antigen References
  1. Feng Y, et al. 2018. Cancer Med. 7:6124-6136.
  2. Sharma, et al. 2022. Semin Cancer Biol. 87:184-195. 
  3. Urbanska K and Orzechowski A. 2019. Int J Mol Sci 20:2085. 
  4. Kraus AP and Neely CJ. 1964. Science. 145:595-7.
Gene ID
3939 View all products for this Gene ID
UniProt
View information about LDHA on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All LDHA Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-LDHA A22115F WB,IHC-P,ICC,ICFC
Go To Top Version: 1    Revision Date: 05.29.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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